JOURNAL ARTICLE

Functional annotation of a full-length Arabidopsis cDNA collection

Motoaki Seki, Mari Narusaka, Asako Kamiya, Junko Ishida, Masakazu Satou, Tetsuya Sakurai, Maiko Nakajima, Akiko Enju, Kenji Akiyama, Youko Oono, Masami Muramatsu, Yoshihide Hayashizaki, Jun Kawai, Piero Carninci, Masayoshi Itoh, Yoshiyuki Ishii, Takahiro Arakawa, Kazuhiro Shibata, Akira Shinagawa, Kazuo Shinozaki
Science 2002 April 5, 296 (5565): 141-5
11910074
Full-length complementary DNAs (cDNAs) are essential for the correct annotation of genomic sequences and for the functional analysis of genes and their products. We isolated 155,144 RIKEN Arabidopsis full-length (RAFL) cDNA clones. The 3'-end expressed sequence tags (ESTs) of 155,144 RAFL cDNAs were clustered into 14,668 nonredundant cDNA groups, about 60% of predicted genes. We also obtained 5' ESTs from 14,034 nonredundant cDNA groups and constructed a promoter database. The sequence database of the RAFL cDNAs is useful for promoter analysis and correct annotation of predicted transcription units and gene products. Furthermore, the full-length cDNAs are useful resources for analyses of the expression profiles, functions, and structures of plant proteins.

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