We have located links that may give you full text access.
Optimized conditions for the long-term growth of primary cell cultures derived from the Asian citrus psyllid, Diaphorina citri (Liviidae: Hemiptera).
In Vitro Cellular & Developmental Biology. Animal 2023 April 22
The Asian citrus psyllid (ACP), Diaphorina citri Kuwayama (Hemiptera: Liviidae), is a pest of significant importance to global citrus production, particularly as the vector of a phloem-limited bacterium Candidatus Liberibacter asiaticus (CLas) that causes the fatal citrus disease Huanglongbing or citrus greening. CLas is acquired as the psyllid feeds, replicates in ACP tissues, and persists throughout the life of the insect. The study of CLas has been hampered by the lack of a tractable in vitro culture system. As CLas replicates within psyllid tissues, we hypothesize that this bacterium also replicates in cultured ACP cells. In the current study, we evaluated a range of insect cell culture media, media combinations, and supplements for their ability to support the in vitro growth of ACP embryo-derived cells. Ninety-six primary cell cultures were initiated using approximately 12,000 dissected ACP eggs over a 12-month period. Of 19 media tested, 17 supported cell attachment, but only two media supported the long-term survival and growth of ACP embryonic cells over a period of more than 11 months. Delineation of the optimal protocols and conditions for the maintenance of ACP primary cultures as described here provides a foundation for both establishment of continuous cell lines and testing for the replication of ACP-associated pathogens including CLas.
Full text links
Related Resources
Trending Papers
A Systematic Review of Subclinical Hyperthyroidism Guidelines: a Remarkable Range of Recommendations.European Thyroid Journal 2024 May 2
Nutrition in the intensive care unit: from the acute phase to beyond.Intensive Care Medicine 2024 May 22
The Therapy and Management of Heart Failure with Preserved Ejection Fraction: New Insights on Treatment.Cardiac Failure Review 2024
Sodium-glucose co-transporter protein 2 (SGLT2) inhibitors for people with chronic kidney disease and diabetes.Cochrane Database of Systematic Reviews 2024 May 22
Bronchiectasis management in adults: state of the art and future directions.European Respiratory Journal 2024 May 24
Pathophysiological Link and Treatment Implication of Heart Failure and Preserved Ejection Fraction in Patients with Chronic Kidney Disease.Biomedicines 2024 April 31
2024 update in heart failure.ESC Heart Failure 2024 May 29
Get seemless 1-tap access through your institution/university
For the best experience, use the Read mobile app
All material on this website is protected by copyright, Copyright © 1994-2024 by WebMD LLC.
This website also contains material copyrighted by 3rd parties.
By using this service, you agree to our terms of use and privacy policy.
Your Privacy Choices
You can now claim free CME credits for this literature searchClaim now
Get seemless 1-tap access through your institution/university
For the best experience, use the Read mobile app