Kodavala Sireesha, Echambadi Loganathan Samundeshwari, Kattaru Surekha, Chodimella Chandrasekhar, Potukuchi Venkata Gurunadha Krishna Sarma
The epidermis is largely composed of keratinocytes (KCs), and the proliferation and differentiation of KCs from the stratum basale to the stratum corneum is the cellular hierarchy present in the epidermis. In this study, we explore the differentiation abilities of human hematopoietic stem cells (HSCs) into KCs. Cultured HSCs positive for CD34, CD45, and CD133 with prominent telomerase activity were induced with keratinocyte differentiation medium (KDM), which is composed of bovine pituitary extract (BPE), epidermal growth factor (EGF), insulin, hydrocortisone, epinephrine, transferrin, calcium chloride (CaCl2 ), bone morphogenetic protein 4 (BMP4), and retinoic acid (RA)...
March 19, 2024: In Vitro Cellular & Developmental Biology. Animal