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In Vitro Cellular & Developmental Biology. Animal

Hui Qi, Dan-Ping Liu, Da-Wei Xiao, Da-Chuan Tian, Yong-Wei Su, Shao-Feng Jin
Osteoarthritis (OA) is the most common chronic joint disease worldwide. Chondrocyte, as the only resident cell type in cartilage, its apoptosis is of pathogenetic significance in OA. Mesenchymal stem cell (MSC)-based-therapy has been proved effective in OA in animals and clinical studies. Nowadays, the regenerative potential of MSC-based therapy is mostly attributed to its paracrine secretion, in which exosomes may play an important role. In the present study, we aimed to find out the significance of MSC-derived exosomes (MSC-Exos) on the viability of chondrocytes under normal and inflammatory conditions...
February 19, 2019: In Vitro Cellular & Developmental Biology. Animal
Miyuki Yokoi, Koh-Ichi Kuremoto, Shinsuke Okada, Miwa Sasaki, Kazuhiro Tsuga
Attenuation of fibroblast growth factor receptor (FGFR) 2b signaling suppresses the differentiation of oral epithelial stem cells to ameloblasts, their survival and viability remaining unaffected; however, its effect on dentin formation is unknown. This study aimed to clarify the effect of attenuation of FGFR2b signaling on odontoblast differentiation and dentin formation. Initially, we used a murine rtTA transactivator/tetracycline promoter system for inducible and reversible attenuation of FGFR2b signaling in adult mice...
February 12, 2019: In Vitro Cellular & Developmental Biology. Animal
Stephanie Munroe, Kenneth Sandoval, Dirk E Martens, Detmer Sipkema, Shirley A Pomponi
Sponges are rich sources of novel natural products. Production in cell cultures may be an option for supply of these compounds but there are currently no sponge cell lines. Because there is a lack of understanding about the precise conditions and nutritional requirements that are necessary to sustain sponge cells in vitro, there has yet to be a defined, sponge-specific nutrient medium. This study utilized a genetic algorithm approach to optimize the amino acid composition of a commercially available basal cell culture medium in order to increase the metabolic activity of cells of the marine sponge Dysidea etheria...
February 11, 2019: In Vitro Cellular & Developmental Biology. Animal
Xiangdong Liu, Wenzhong Zhu, Lei Wang, Jin Wu, Feng Ding, Yingliang Song
Adipose-derived stem cells (ADSCs) provide a novel method for bone tissue regeneration, but their adipogenic tendency limits their therapeutic efficacy. MicroRNAs (miRNAs) have been reported to regulate stem cell differentiation and bone tissue regeneration, but the detailed mechanism is poorly investigated. Our study indicated that inhibition of miR-145-5p enhanced the osteogenic potential of ADSCs and reduced the adipogenic differentiation. Osteogenesis- and adipogenesis-associated genes were detected by qRT-PCR indicating a corresponding result...
February 11, 2019: In Vitro Cellular & Developmental Biology. Animal
Diksha Dua, D Nagoorvali, M S Chauhan, P Palta, P Mathur, M K Singh
Parthenogenetically developed embryos are efficient sources of in vitro embryo production, having less ethical issue and being useful for investigating culture conditions/treatments, early developmental, genomic studies, and homonymous source of stem cells. Keeping its advantages in mind, we aimed to study the effects of different activating agents on embryo production and its quality and gene expression. In the present study, 1348 immature oocytes recovered were parthenogenetically developed to embryos. Usable-quality immature oocytes were collected by puncturing the surface follicles and matured in in vitro maturation (IVM) medium for 27 h in a humidified 5% CO2 incubator at 38...
February 8, 2019: In Vitro Cellular & Developmental Biology. Animal
Xiaochuan Tang, Jun Shi, Xiaolian Qin, Ning Xiao, Rongyang Li, Hao Hu, Fengshuo Yang, Deshun Shi, Xiaoli Wang
Chicken blastodermal cells (BCs) are pluripotent stem cells derived from early embryos and may be easily obtained and manipulated. However, in vitro cultured BCs have extremely low germline capacity, which may limit their applications. Research on the germ cell differentiation of mammalian pluripotent cells using chemical-inducing agents has gained popularity, and tremendous achievements have been made. Whether chemical-inducing agents allow acquirement of germline competence in BCs is, however, questionable...
February 8, 2019: In Vitro Cellular & Developmental Biology. Animal
Min Seong Kim, Min Hee Park, Ji Eun Park, Jung Im Yun, Jung Hoon Choi, Eunsong Lee, Seung Tae Lee
Spermatogonial stem cells (SSCs) are a useful tool for the generation of genetically modified transgenic sperm. As a result, the transfer of specific genes into the cytoplasm of SSCs is crucial for the successful generation of transgenic sperm. Here, we report electroporation conditions optimized for SSCs derived from the porcine testis. The highest transfection efficiency and cell viability were observed in porcine SSCs transfected with 1 μg transgenic vector with a single electric pulse from an electroporator at a voltage of 100 V and a capacitor setting of 250 μF...
February 6, 2019: In Vitro Cellular & Developmental Biology. Animal
Ann M Fallon
Factors that influence establishment of Wolbachia, an obligate intracellular bacterium, in novel insect hosts or uninfected insect cell lines are poorly understood. Infectivity of Wolbachia strain wStr was correlated with flow cytometric profiles to define optimal conditions for harvesting an infectious inoculum. Wolbachia recovered from the cell culture supernatant after gentle pipetting of infected cells represented about 1% of the total bacterial population and were more infectious than Wolbachia that remained associated with intact cells and/or membranes after low-speed centrifugation...
January 23, 2019: In Vitro Cellular & Developmental Biology. Animal
Aimi Naim Abdullah, Satoshi Miyauchi, Azusa Onishi, Kotaro Tanimoto, Koichi Kato
Recent studies have successfully generated tooth-like structure by mimicking the reciprocal interaction between dental epithelial and mesenchymal cells in tooth organogenesis. However, clinical applications of these methods are limited primarily due to the lack of appropriate sources for dental epithelial cells. Induced pluripotent stem cells (iPSCs) are attractive as a source for dental epithelial cells due to their unique characteristics. In this study, we examined the effect of neurotrophin-4 (NT-4) on the differentiation of mouse iPSCs (miPSCs) into dental epithelial cells...
January 18, 2019: In Vitro Cellular & Developmental Biology. Animal
Ekta Minocha, Chandra Prakash Chaturvedi, Soniya Nityanand
The aim of the present study was to investigate the renogenic characteristics of amniotic fluid stem cells (AFSCs) and to evaluate their in vitro differentiation potential into renal proximal tubular-like cells and juxtaglomerular-like cells. We culture expanded AFSCs derived from rat amniotic fluid. The AFSCs grew as adherent spindle-shaped cells and expressed mesenchymal markers CD73, CD90, and CD105 as well as renal progenitor markers WT1, PAX2, SIX2, SALL1, and CITED1. AFSCs exhibited an in vitro differentiation potential into renal proximal tubular epithelial-like cells, as shown by the upregulation of expression of proximal tubular cell-specific genes like AQP1, CD13, PEPT1, GLUT5, OAT1, and OCT1...
January 15, 2019: In Vitro Cellular & Developmental Biology. Animal
Ji Hun Lee, Seung Tae Lee, Yoon Kwon Nam, Seung Pyo Gong
The optimal transfection conditions for efficient transgene delivery into a specific cell type should be empirically determined, particularly in cases involving unusual cell types. We compared the conditions for effective introduction of transgenes into Siberian sturgeon (Acipenser baerii) cell lines by evaluating the cytotoxicity and transfection efficiency of three commercially available transfection reagents: Lipofectamine 2000, X-tremeGENE HP DNA Transfection Reagent, and GeneJuice Transfection Reagent...
January 14, 2019: In Vitro Cellular & Developmental Biology. Animal
Khudishta Aktar, Abdul Kafi, Ravinder Dahiya
This research demonstrates fluctuation of glutathione peroxidase1 (Gpx1) throughout cell cycle progression with significant decreased expression at mitosis of HeLa cell. This was achieved with western blot (WB) analysis of target proteins from each phase of synchronized cells. The synchronizations were performed with double thymidine (T/T) for G1/S arrest and thymidine followed by nocodazole (T/N) for G2/M arrest. The G1/S arrested cells were released in fresh medium for 3, 6, 9, 10, and 15h to obtain cell at each phase such as gap1 (G1), synthesis (S), gap2 (G2), mitosis (M), and gap1 (G1) phase, respectively, for investigating Gpx1 expression throughout a complete cycle...
January 10, 2019: In Vitro Cellular & Developmental Biology. Animal
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No abstract text is available yet for this article.
January 7, 2019: In Vitro Cellular & Developmental Biology. Animal
Song Chen, Chuanming Dong, Jinyun Zhang, Baohua Tang, Zhengrong Xi, Fei Cai, Yachi Gong, Jianru Xu, Longju Qi, Qinghua Wang, Jian Chen
Human menstrual blood-derived mesenchymal stem cells (MenSCs) hold great promise for regenerative medicine. Here, H2 O2 -associated damage in H9c2 cells was employed as an in vitro ischemia-reperfusion model, and the transwell system was used to explore the beneficial effects of MenSCs on the H2 O2 -induced damage of myocardial H9c2 cells. H2 O2 treatment resulted in decreased viability and migration rate, with increased apoptosis levels in cells. By contrast, upon co-culture with MenSCs, H9c2 cell viability and migration were increased, whereas the apoptotic rate decreased...
January 7, 2019: In Vitro Cellular & Developmental Biology. Animal
Pinar Buket Atalay, Gamze Kuku, Bilge Guvenc Tuna
There has been a controversy in the oncology field about the use of antioxidants along with chemotherapeutics in cancer treatment. This study aimed to investigate the effects of a potent antioxidant (astaxanthin) co-treatment with a promising anti-cancer drug (carbendazim), which is in phase I clinical trials, on MCF-7 breast cancer cell proliferation. MCF-7 cells were treated with carbendazim, astaxanthin, or their combinations and incubated for 24 h. After the incubation, each treatment group was evaluated for proliferation, cell cycle progression, and production of reactive oxygen species (ROS) using WST-1, flow cytometry, and CM-H2DCFDA, respectively...
December 13, 2018: In Vitro Cellular & Developmental Biology. Animal
Obaid Ullah, Zhongshu Li, Ihsan Ali, Lijie Xu, Haixing Liu, Hua-Zhi Jin, Yan-Ya Fang, Qing-Guo Jin, Nanzhu Fang
Pterostilbene (PTS) mainly enriched in small fruits such as berries and grapes exerts an antioxidant effect. However, the protective effects of PTS against endoplasmic reticulum stress (ERS) have not yet been elucidated in mouse preimplantation embryo. ERS plays an important role in regulating the pathological and physiological processes, including embryonic development. We explored the protective effect of PTS on the tunicamycin (TM)-induced ERS in mouse preimplantation embryos. In vitro, culture medium was supplemented with different concentrations of TM and PTS...
December 13, 2018: In Vitro Cellular & Developmental Biology. Animal
Yuki Ohmuro-Matsuyama, Tomohisa Katsuda, Hideki Yamaji
Monoclonal antibodies and antibody fragments are widely used in therapeutics and diagnoses. While mammalian cells serve as the host cells for antibody production, insect cells can produce large quantities of secretory antibodies in serum-free suspension cultures. The effects of lithium on the processes of autophagy and apoptosis in mammalian cells are well chronicled. In the present study, stably transformed insect cells, which produce an engineered antibody molecule, were cultured with lithium chloride in a serum-free medium...
January 2019: In Vitro Cellular & Developmental Biology. Animal
Rongqun Li, Aiwen Zhuang, Jiawei Ma, Lina Ji, Xiaoli Hou, Hongbo Chen, Xiaoping Pan, Wenhong Liu
How genomic DNA methylation and methyl CpG-binding protein 2 (MeCP2) gene expression affect the pathogenesis of systemic lupus erythematosus (SLE) remains poorly understood. Traditional Chinese medicine has a unique effect in the treatment of SLE patients. This study aimed to investigate the effect of Jieduquyuziyin prescription (JP)-treated rat serum on the gene expression of MeCP2 in Jurkat T cells and its role in the pathogenesis of SLE. Jurkat T cells were harvested, and drug-containing serum was prepared...
December 2018: In Vitro Cellular & Developmental Biology. Animal
Hongwei Zhang, Yaofa Li, Tamra Reall, Yiyun Xu, Cynthia Goodman, Stephen Saathoff, Joseph Ringbauer, David Stanley
Spodoptera eridania (southern armyworm) is a polyphagous pest of many plants, including field crops, vegetables, fruits, and ornamentals. Larvae are leaf feeders, defoliating many crops in the tropics and subtropics of the western hemisphere. In this study, cell lines from S. eridania were established to support research focused on the development of advanced pest management technologies. We generated seven cell lines from larval tissues: three from nervous tissues, two from testes, and two from fat bodies...
December 2018: In Vitro Cellular & Developmental Biology. Animal
Xiaolong Yuan, Xi Deng, Xiaofeng Zhou, Ailing Zhang, Yan Xing, Zhe Zhang, Hao Zhang, Jiaqi Li
In mammalian ovaries, many studies demonstrated that the proliferation and apoptosis of granulosa cells are involved in folliculogenesis. Previous evidence suggests that miR-126-3p might get involved in the proliferation and apoptosis of granulosa cells, and tuberous sclerosis complex 1 (TSC1) gene was predicted as one target of miR-126-3p, and moreover, granulosa cell-specific TSC1 knockout stimulated folliculogenesis in mice. However, the molecular regulation of miR-126-3p on TSC1 and its effects on cell proliferation and apoptosis remain virtually unexplored in granulosa cells...
December 2018: In Vitro Cellular & Developmental Biology. Animal
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