Determining Ultra-Low Organic Molecular Odor Thresholds in Air Helps Identify the Most Potent Fungal Aroma Compound.

The determination of odor threshold values can be performed in various matrices, including air, and serves as a parameter to compare the potencies of odorous compounds. Typically, the odor thresholds in air are determined by gas chromatography-olfactory (GC-O) and referenced to an internal standard, most often ( E )-dec-2-enal. Herein, a direct gas chromatography-flame ionization detector-olfactory analysis method for the determination of odor thresholds in air is reported. As model substrates for this novel approach, naturally occurring substances ( R )-1- p -menthene-8-thiol as well as (3 S ,3a S ,6 R ,7a S )-3,6-dimethyl-3a,4,5,6,7,7a-hexahydro-3 H -1-benzofuran-2-one were used. The latter compound was synthesized from (-)-isopulegol and exhibited an extremely low odor recognition threshold of 1.9 × 10-6 ng L-1 air, the lowest value reported for a fungal aroma compound thus far.