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Journal of Agricultural and Food Chemistry

Xi-Xiang Tang, Xia Yan, Wen-Hao Fu, Lu-Qi Yi, Bo-Wen Tang, Li-Bo Yu, Meijuan Fang, Zhen Wu, Ying-Kun Qiu
Fusarium solani H915 (MCCC3A00957), a fungus originating from mangrove sediment, showed potent inhibitory activity against tea pathogenic fungus Pestalotiopsis theae. Successive chromatographic separation on an ethyl acetate (EtOAc) extract of F. solani H915 resulted in the isolation of five new alkenoic diacid derivatives, fusarilactones A-C (1-3), fusaridioic acids B (4) and C (5), in addition to seven known compounds (6-12). The chemical structures of these metabolites were elucidated on the basis of UV, IR, HR-ESI-MS and NMR spectroscopic data...
February 20, 2019: Journal of Agricultural and Food Chemistry
Laifeng Lu, Lifeng Ji, Qingqing Ma, Mingguan Yang, Shuhua Li, Qiong Tang, Liping Qiao, Fengjuan Li, Qingbin Guo, Changlu Wang
The acquisition of susceptibility to necrotrophy over the course of ripening is one of the critical factors limiting shelf life. In this study, phytopathology and molecular biology were employed to explore the roles of pectinase in fruit susceptibility and ripening. Solanum lycopersicum fruit softened dramatically from entirely green to 50% red, which was accompanied by a continuously higher expressed SlPG2 gene. The necrotrophic fungus Botrytis cinerea further activated the expression of SlPGs and SlPMEs to accelerate cell wall disassembly, while most of the polygalacturonase inhibitor proteins encoding genes expression were postponed in ripe fruit following the pathogen attack...
February 20, 2019: Journal of Agricultural and Food Chemistry
Zhimei Tian, Xianyong Ma, Dun Deng, Yiyan Cui, Weidong Chen
To investigate effects of dietary nitrogen level on nutrient absorption and utilization in small intestinal enterocyte of piglets , weaned piglets were fed for 10 d with diets containing 20%, 17%, or 14% crude protein (CP) with supplementation to meet requirements for essential amino acids in vivo, and IPEC-1 cells were cultured with different nitrogen levels (NL) in culture medium (70%, 85%, 100%) in vitro by mono-cultured and co-cultured of IPEC-1 and GES-1 cells. The results showed that: (1) In animal trail, decreased dietary CP reduced transcript abundance of nutrient transporters like CAT1, PepT1, GLUT2 and SGLT-1 in jejunal mucosa (P < 0...
February 20, 2019: Journal of Agricultural and Food Chemistry
Xiaohui Zhao, Jihong Tao, Ting Zhang, Sirong Jiang, Wei Wei, Hongping Han, Yun Shao, Guoying Zhou, Huilan Yue
The regulation of postprandial blood glucose (PBG) levels is an effective therapeutic method to treat diabetes and prevent diabetes-related complications. Resveratroloside is a mono-glucosylated form of stilbene that is present in red wine, grapes and several traditional medicinal plants. In our study, the effect of resveratroloside on reducing PBG was studied in vitro and in vivo. Compared with the starch treatment alone, the oral administration of resveratroloside-starch complexes significantly inhibited the PBG increase in a dose-dependent pattern in normal and diabetic mice...
February 20, 2019: Journal of Agricultural and Food Chemistry
Kefeng Zhai, Hong Duan, Cai-Yue Cui, Yu-Yao Cao, Jia-Li Si, Hui-Jiao Yang, Yong-Chao Wang, Wen-Gen Cao, Gui-Zhen Gao, Zhao-Jun Wei
Among the various treatments, induction of synoviocytes apoptosis by natural products during rheumatoid arthritis (RA) pathological condition can be considered of vast potential. However, it is unclear that liquiritin, a kind of natural flavonoid extracted from the roots of Glycyrrhiza uralensis, induced the apoptosis of synovial membrane and its molecular mechanism. In this study, interleukin-1β (IL-1β)-RA-FLS cells were incubated with different concentration of liquiritin. MTT assay, Hoechst 33342 staining, JC-1 staining and western blot were used to check the viability, cell apoptosis, mitochondrial membrane potential changes and the expression of related proteins, respectively...
February 20, 2019: Journal of Agricultural and Food Chemistry
Diego Luna-Vital, Laura Chatham, John Juvik, Vijay Singh, Pavel Somavat, Elvira Gonzalez de Mejia
The aim was to characterize a phenolic-rich water extract from the pericarp of an improved genotype of Apache red maize (RPE), and evaluate its ability to activate the type-2 diabetes markers free fatty acid receptor 1 (GPR40) and glucokinase (GK) in vitro. The extract contained mainly phenolic acids, anthocyanins, and other flavonoids. RPE inhibited α-amylase (IC50 = 88.3 µg/mL), α-glucosidase (IC50 = 169.3 µg/mL), and reduced glucose transport in a Caco-2 cell monolayer (up to 25%). Furthermore, RPE activated GPR40 (EC50 = 77...
February 20, 2019: Journal of Agricultural and Food Chemistry
Delong Zeng, Jianfu Zhao, Kar-Him Luk, Siu-To Cheung, Ka Hing Wong, Tianfeng Chen
Selenium nanoparticles (SeNPs) are recently emerging as promising anticancer agents because of their high bioavailability, low toxicity and remarkable anticancer activities. However, the effects of surface physicochemical properties on the biological actions remain elusive. Herein we decorated selenium nanoparticles (SeNPs) with various water-soluble polysaccharides extracted from various mushrooms, to compare physical characteristics and anticancer profile of these SeNPs. The results showed that the prepared spherical SeNPs displayed particle sizes at 91-102 nm, and kept stable in aqueous solution for up to 13 weeks...
February 20, 2019: Journal of Agricultural and Food Chemistry
Benjamin W Redan, Joseph E Jablonski, Catherine Halverson, James Jaganathan, Md Abdul Mabud, Lauren S Jackson
Filtration methods for alcoholic fermented beverages often use filter aids such as diatomaceous earth (DE), which may contain elevated amounts of the heavy metals arsenic (As), lead (Pb), and cadmium (Cd). Here, we evaluated factors affecting transfer of these heavy metals from DE to beer and wine. A laboratory-scale filtration system was used to process unfiltered ale, lager, red wine, and white wine with three types of food-grade DE. Filtrate and DE were analyzed for heavy metals using ICP-MS, in addition to LC-ICP-MS for As-speciation analysis...
February 20, 2019: Journal of Agricultural and Food Chemistry
Dana M Poloni, Olivier Dangles, Joe A Vinson
Cardiovascular disease (CVD) is the leading cause of death in industrialized nations. The initiating event in atherosclerosis or hardening of the arteries, is oxidation of low density lipoprotein (LDL). Binding with serum albumin and LDL of 41 polyphenols (major antioxidants in plant foods) constituting four classes of flavonoids, three types of phenolic acids and seven polyphenol conjugate metabolites was investigated indirectly by fluorescence quenching and directly by affinity separation/HPLC (four of the polyphenols)...
February 20, 2019: Journal of Agricultural and Food Chemistry
Tomoko Asai, Akira Takahashi, Kumie Ito, Tatsuo Uetake, Yasuki Matsumura, Kaori Ikeda, Nobuya Inagaki, Masahiro Nakata, Yoshiharu Imanishi, Kenji Sato
Objectives of the present study were to evaluate amounts of collagen in Japanese daily dishes and contents of food-derived collagen peptides in human blood. The meat in one serving of most Japanese daily dishes contains 0.2-2.5 g of collagen, except for beef tendon, eel with skin, and skinned shark tail (7.6-13.3 g). After ingestion of cooked shark meat, nine collagen di- and tri-peptides were detected in plasma and the area under the curve of most peptides, except for Hyp-Gly and Pro-Hyp-Gly, was ~30% of that after ingestion of collagen hydrolysate containing an equivalent amount of collagen...
February 20, 2019: Journal of Agricultural and Food Chemistry
Kiira Susanna Vuoristo, Lasse Fredriksen, Maren Oftebro, Magnus Øverlie Arntzen, Olav Andreas Aarstad, Runar Stokke, Ida H Steen, Line Degn Hansen, Reidar Barfod Schüller, Finn Lillelund Aachmann, Svein J Horn, Vincent G H Eijsink
Enzymatic depolymerization of seaweed polysaccharides is gaining interest for the production of functional oligosaccharides and fermentable sugars. We describe a thermostable alginate lyase belonging to Polysaccharide Lyase family 7 (PL7), which can be used to degrade brown seaweed, Saccharina latissima, at conditions also suitable for a commercial cellulase cocktail (Cellic CTec2). This enzyme, AMOR_PL7A, is a β-D-mannuronate specific (EC endo-acting alginate lyase, which degrades alginate and poly-mannuronate within a broad range of pH, temperature and salinity...
February 20, 2019: Journal of Agricultural and Food Chemistry
Shaohua Li, Dawei Chen, Bing Lv, Jingguang Li, Yunfeng Zhao, Yongning Wu
Extensive residues of neonicotinoids (neonics) have been demonstrated in food and environment by routine monitoring measurement, but little is known about the residue levels in "ready to eat" dietary samples. To obtain a more accurate picture of dietary exposure to total neonics, an ultra-sensitive and effective cleanup analytical method for the quantification of neonics in dietary samples was established based on cold-induced phase separation and pre-column dilution injection (PDI) liquid chromatography-high resolution mass spectrometry (LC-HRMS)...
February 19, 2019: Journal of Agricultural and Food Chemistry
Anke R Boeswetter, Katharina Anne Scherf, Peter Schieberle, Peter Koehler
Rice flour is commonly used as raw material in the preparation of gluten-free bread. However, compared to wheat bread, its aroma is different and often not accepted by consumers. Aroma profile analyses of the crumb and crust of freshly baked rice bread indicated a strong rice-like flavor, but lower intensities of the typical roasty wheat bread odor qualities. By application of aroma extract dilution analyses (AEDA), the most odor active compounds in both, rice and wheat bread samples were characterized. In addition, two batches of rice flour were used...
February 19, 2019: Journal of Agricultural and Food Chemistry
Shen Zhuang, Ruyang Yu, Jia Zhong, Ping Liu, Zhongjie Liu
Rheum rhabarbarum has been widely used as a herbal medicine and food in China. The objective of this study was to investigate the cytoprotective action and underlying mechanisms of rhein, one active ingredient isolated from Rheum rhabarbarum, on H2O2-challenged rat small intestine epithelial cells (IEC-6 cells). H2O2-challenged IEC-6 cells were incubated in the pretreatment with or without rhein or LY294002, a PI3K/Akt inhibitor. The cell viability, apoptosis, intracellular ROS and antioxidants were measured...
February 19, 2019: Journal of Agricultural and Food Chemistry
Fuguo Liu, Hua Liu, Runhua Liu, Chunxia Xiao, Xiang Duan, David Julian McClements, Xuebo Liu
Anticancer nanoparticles were fabricated by linking the nanoparticles of two known anticancer agents, sesamol and selenium, using polyethylene glycol (PEG). The successful fabrication of the sesamol-PEG-selenium nanoparticles (PEG-SeNPs), which had a sesamol loading efficiency of 10.0 ± 0.5 wt%, was demonstrated using different spectroscopic techniques. The impact of the nanoparticles on model cancer cells (HepG2) was established using cell activity test, morphological observation and fluorescent staining, which all showed the nanoparticles effectively inhibited the HepG2 cells...
February 19, 2019: Journal of Agricultural and Food Chemistry
Fannie Thibaud, Svitlana Shinkaruk, Philippe Darriet
Cognac Wine Distillate (WD), especially during their aging, are marked by complex and elegant aroma. This work aimed at expanding the knowledge on the Cognac WD aroma by a sensory-guided approach, involving fractional distillation technique and gas chromatography coupled to olfactometry and mass spectrometry (GC-O-MS). In doing so, a fruity odorous zone was highlighted in WD extracts which was attributed to the diethyl acetal family. Ten additional diethyl acetals were detected by GC-MS. Next, an assay method was developed and validated for seven of these diethyl acetals...
February 19, 2019: Journal of Agricultural and Food Chemistry
Chandran Sathesh-Prabu, Sung Kuk Lee
Monomeric 4-hydroxyvalerate is a versatile chemical used to produce various commodities and fine chemicals. In the present study, the lvaAB gene was deleted from the lva operon in Pseudomonas putida KT2440 and the tesB, obtained from Escherichia coli, was overexpressed under the control of the lva operon system that is inducible by substrate levulinic acid and product 4-hydroxyvalerate to produce 4-hydroxyvalerate from levulinic acid. The lvaAB deleted strain showed almost complete conversion of levulinic acid to 4-hydroxyvalerate, compared with 24% conversion in the wild type strain...
February 18, 2019: Journal of Agricultural and Food Chemistry
Huertas M Diaz-Mula, Francisco A Tomas-Barberan, Rocio Garcia-Villalba
Proanthocyanidins (PAs) were characterized in husk, membranes, arils and seeds of 'Mollar de Elche' pomegranate cultivar using LC-ESI-IT MS/MS after acid-catalysis in the presence of phloroglucinol (phloroglucinolysis). PAs were also evaluated in commercial juice and in the juice made in the laboratory by pressing fresh arils. Pomegranate PAs were composed of a very rich mixture of flavan-3-ols: catechin, epicatechin, gallocatechin, epigallocatechin, epigallocatechin gallate and epicatechin gallate. Catechins and gallocatechins were the most abundant units while galloylated flavanols were detected at lower concentrations...
February 15, 2019: Journal of Agricultural and Food Chemistry
Wenqiang Tan, Fang Dong, Jingjing Zhang, Xiang Zhao, Qing Li, Zhanyong Guo
Chitosan ascorbates with different substitution degrees were synthesized based on salification of chitosan and ascorbic acid at various molar ratios in water and were successfully used to prepare antioxidative films by casting for the first time. FTIR and 1H NMR spectra recorded the structural characteristics of all chitosan ascorbates, meanwhile physicochemical property and antioxidant activity of the produced chitosan ascorbate films were characterized and chitosan acetate film served as control were also measured these properties for comparison...
February 15, 2019: Journal of Agricultural and Food Chemistry
Eun-Hye Choi, Chan-Su Rha, Sri Renukadevi Balusamy, Dae-Ok Kim, Soon-Mi Shim
Two bioconversions were applied to green tea extracts (GTE) and flavonol glycoside rich fraction (FVNg) derived from insoluble green tea extract by tannase and cellulase treatment in order to obtain gallated catechins (EnzGTE) and flavonol aglycone rich fraction (FVNa), respectively. The bioaccessibility of epicatechins from GTE increased with the addition of FVNg, FVNa, and flavonol aglycone rich fraction of commercial production (FVNap). Epigallocatechin-gallate (EGCG) and epicatechin-gallate (ECG) were highly recovered 4- and 125-fold, respectively, by adding FVNap...
February 15, 2019: Journal of Agricultural and Food Chemistry
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