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Journals International Journal of Molec...

International Journal of Molecular Sciences

https://read.qxmd.com/read/38542536/immunotherapy-and-cancer-the-multi-omics-perspective
#1
REVIEW
Clelia Donisi, Andrea Pretta, Valeria Pusceddu, Pina Ziranu, Eleonora Lai, Marco Puzzoni, Stefano Mariani, Elena Massa, Clelia Madeddu, Mario Scartozzi
Immunotherapies have revolutionized cancer treatment approaches. Because not all patients respond positively to immune therapeutic agents, it represents a challenge for scientists who strive to understand the mechanisms behind such resistance. In-depth exploration of tumor biology, using novel technologies such as omics science, can help decode the role of the tumor immune microenvironment (TIME) in producing a response to the immune blockade strategies. It can also help to identify biomarkers for patient stratification and personalized treatment...
March 21, 2024: International Journal of Molecular Sciences
https://read.qxmd.com/read/38542535/therapeutic-applications-of-stem-cell-derived-exosomes
#2
REVIEW
Omar Abdulhakeem Ahmed Yusuf Abdulmalek, Khaled Hameed Husain, Haya Khaled Ali Abdulla AlKhalifa, Mariam Masood Abdulkarim Bahrooz Alturani, Alexandra E Butler, Abu Saleh Md Moin
Exosomes are extracellular vesicles of endosomal origin, ranging from 30 to 150 nm in diameter, that mediate intercellular transfer of various biomolecules, such as proteins, lipids, nucleic acids, and metabolites. They modulate the functions of recipient cells and participate in diverse physiological and pathological processes, such as immune responses, cell-cell communication, carcinogenesis, and viral infection. Stem cells (SCs) are pluripotent or multipotent cells that can differentiate into various cell types...
March 21, 2024: International Journal of Molecular Sciences
https://read.qxmd.com/read/38542534/tocotrienols-prevent-the-decline-of-learning-ability-in-high-fat-high-sucrose-diet-fed-c57bl-6-mice
#3
JOURNAL ARTICLE
Yugo Kato, Junhyoku Ben, Atsuto Noto, Shuntaro Kashiwaya, Yoshinori Aoki, Nobuo Watanabe, Hiroki Tsumoto, Yuri Miura, Koji Fukui
Obesity has been increasing worldwide and is well-known as a risk factor for cognitive decline. It has been reported that oxidative stress in the brain is deeply involved in cognitive dysfunction in rodent models. While there are many studies on oxidation in the liver and adipose tissue of obese mice, the relationship between obesity-induced cognitive dysfunction and brain oxidation has not been elucidated. Here, we show that obesity induced by a high-fat, high-sucrose diet (HFSD) alters cognitive function in C57BL/6 male mice, and it may involve the acceleration of brain oxidation...
March 21, 2024: International Journal of Molecular Sciences
https://read.qxmd.com/read/38542533/extracellular-vesicle-protein-expression-in-doped-bioactive-glasses-further-insights-applying-anomaly-detection
#4
JOURNAL ARTICLE
Mauro Nascimben, Hugo Abreu, Marcello Manfredi, Giuseppe Cappellano, Annalisa Chiocchetti, Lia Rimondini
Proteomic analysis of extracellular vesicles presents several challenges due to the unique nature of these small membrane-bound structures. Alternative analyses could reveal outcomes hidden from standard statistics to explore and develop potential new biological hypotheses that may have been overlooked during the initial evaluation of the data. An analysis sequence focusing on deviating protein expressions from donors' primary cells was performed, leveraging machine-learning techniques to analyze small datasets, and it has been applied to evaluate extracellular vesicles' protein content gathered from mesenchymal stem cells cultured on bioactive glass discs doped or not with metal ions...
March 21, 2024: International Journal of Molecular Sciences
https://read.qxmd.com/read/38542532/expression-of-placental-lipid-transporters-in-pregnancies-complicated-by-gestational-and-type-1-diabetes-mellitus
#5
JOURNAL ARTICLE
Paweł Jan Stanirowski, Mateusz Wątroba, Michał Pyzlak, Jarosław Wejman, Dariusz Szukiewicz
The objective of the study was to assess the expression of proteins responsible for placental lipid transport in term pregnancies complicated by well-controlled gestational (GDM) and type 1 diabetes mellitus (PGDM). A total of 80 placental samples were obtained from patients diagnosed with PGDM ( n = 20), GDM treated with diet (GDMG1, n = 20), GDM treated with diet and insulin (GDMG2, n = 20), and a non-diabetic control group ( n = 20). Umbilical and uterine artery blood flows were assessed by means of ultrasound in the period prior to delivery and computer-assisted quantitative morphometry of immunostained placental sections was performed to determine the expression of selected proteins...
March 21, 2024: International Journal of Molecular Sciences
https://read.qxmd.com/read/38542531/in-vitro-antibacterial-activity-of-ozonated-olive-oil-against-bacteria-of-various-antimicrobial-resistance-profiles-isolated-from-wounds-of-companion-animals
#6
JOURNAL ARTICLE
Anna Lenart-Boroń, Klaudia Stankiewicz, Klaudia Bulanda, Natalia Czernecka, Miłosz Heliasz, Walter Hunter, Anna Ratajewicz, Karen Khachatryan, Gohar Khachatryan
Frequent colonization and bacterial infection of skin wounds in small animals prevent or impair their healing. However, the broadly applied antimicrobial therapy of wounds is not always necessary and promotes the spread of bacterial resistance. Thus, alternatives to antimicrobial therapy, including preventive measures in the form of wound dressings with antibiotic properties, should be searched for. The aim of this study was to develop a new, efficient, cost-effective and non-toxic formulation with antimicrobial properties to serve as an alternative to antibiotic administration in wound-healing stimulation in companion animals...
March 21, 2024: International Journal of Molecular Sciences
https://read.qxmd.com/read/38542530/design-synthesis-anti-inflammatory-activity-dft-modeling-and-docking-study-of-new-ibuprofen-derivatives
#7
JOURNAL ARTICLE
Abbas M Abbas, Hossam H Nasrallah, Ahmed Aboelmagd, Safaa M Kishk, W Christopher Boyd, Haitham Kalil, Adel S Orabi
A new ibuprofen derivative, ( E )-2-(4-isobutylphenyl)-N'-(4-oxopentan-2-ylidene) propane hydrazide (IA), was synthesized, along with its metal complexes with Co, Cu, Ni, Gd, and Sm, to investigate their anti-inflammatory efficacy and COX-2 inhibition potential. Comprehensive characterization, including 1 H NMR, MS, FTIR, UV-vis spectroscopy, and DFT analysis, were employed to determine the structural configurations, revealing unique motifs for Gd/Sm (capped square antiprismatic/tricapped trigonal prismatic) and Cu/Ni/Co (octahedral) complexes...
March 21, 2024: International Journal of Molecular Sciences
https://read.qxmd.com/read/38542529/high-or-low-yielding-f-2-progeny-of-wheat-is-result-of-specific-tackx-gene-coexpression-patterns-in-association-with-grain-yield-in-paternal-parent
#8
JOURNAL ARTICLE
Karolina Szala, Marta Dmochowska-Boguta, Joanna Bocian, Wacław Orczyk, Anna Nadolska-Orczyk
Members of the TaCKX gene family (GFM) encode oxidase/dehydrogenase cytokinin degrading enzymes (CKX), which play an important role in the homeostasis of phytohormones, affecting wheat development and productivity. Therefore, the objective of this investigation was to test how the expression patterns of the yield-related TaCKX genes and TaNAC2-5A ( NAC2 ) measured in 7 days after pollination (DAP) spikes and the seedling roots of parents are inherited to apply this knowledge in the breeding process. The expression patterns of these genes were compared between parents and their F 2 progeny in crosses of one mother with different paterns of awnless cultivars and reciprocal crosses of awned and awnless lines...
March 21, 2024: International Journal of Molecular Sciences
https://read.qxmd.com/read/38542528/quantitative-shotgun-proteomic-analysis-of-bacteria-after-overexpression-of-recombinant-spider-miniature-spidroin-masp1
#9
JOURNAL ARTICLE
Kathryn Randene, J Alexander Hoang Mendoza, Michael Ysit, Craig Vierra
Spider silk has extraordinary mechanical properties, displaying high tensile strength, elasticity, and toughness. Given the high performance of natural fibers, one of the long-term goals of the silk community is to manufacture large-scale synthetic spider silk. This process requires vast quantities of recombinant proteins for wet-spinning applications. Attempts to synthesize large amounts of native size recombinant spidroins in diverse cell types have been unsuccessful. In these studies, we design and express recombinant miniature black widow MaSp1 spidroins in bacteria that incorporate the N-terminal and C-terminal domain (NTD and CTD), along with varying numbers of codon-optimized internal block repeats...
March 21, 2024: International Journal of Molecular Sciences
https://read.qxmd.com/read/38542527/in-silico-description-of-the-direct-inhibition-mechanism-of-endothelial-lipase-by-angptl3
#10
JOURNAL ARTICLE
Linda Montavoci, Omar Ben Mariem, Simona Saporiti, Tommaso Laurenzi, Luca Palazzolo, Alice Federica Ossoli, Uliano Guerrini, Laura Calabresi, Ivano Eberini
Angiopoietin-like protein 3 (ANGPTL3) is a plasmatic protein that plays a crucial role in lipoprotein metabolism by inhibiting the lipoprotein lipase (LPL) and the endothelial lipase (EL) responsible for the hydrolysis of phospholipids on high-density lipoprotein (HDL). Interest in developing new pharmacological therapies aimed at inhibiting ANGPTL3 has been growing due to the hypolipidemic and antiatherogenic profile observed in its absence. The goal of this study was the in silico characterization of the interaction between ANGPTL3 and EL...
March 21, 2024: International Journal of Molecular Sciences
https://read.qxmd.com/read/38542526/plasma-mir-203a-3p-as-a-novel-predictor-of-dementia-in-patients-with-parkinson-s-disease
#11
JOURNAL ARTICLE
Ya-Fang Hsu, Shau-Ping Lin, Yung-Tsai Chu, Yi-Tzang Tsai, Jing-Wen Huang, Frederick Kin Hing Phoa, Ruey-Meei Wu
The early detection of cognitive decline in Parkinson's disease is important for providing drug therapy and non-pharmacological management. The circulating microRNAs present in plasma are promising biomarkers of PD with dementia (PDD) due to their critical roles in synaptic plasticity and the regulation of neurodegeneration-associated proteins. In this study, we aimed to identify plasma microRNAs that may differentiate PD with or without cognitive impairment. Global microRNA expression was obtained from a discovery set of 123 participants who were divided into four groups, namely normal controls (HC), PD with no dementia (PDND), PD with mild cognitive impairment (PD-MCI), and PDD, using next-generation sequencing...
March 21, 2024: International Journal of Molecular Sciences
https://read.qxmd.com/read/38542525/modeling-lysosomal-storage-disorders-in-an-innovative-way-establishment-and-characterization-of-stem-cell-lines-from-human-exfoliated-deciduous-teeth-of-mucopolysaccharidosis-type-ii-patients
#12
JOURNAL ARTICLE
Sofia Carvalho, Juliana Inês Santos, Luciana Moreira, Ana Joana Duarte, Paulo Gaspar, Hugo Rocha, Marisa Encarnação, Diogo Ribeiro, Matilde Barbosa Almeida, Mariana Gonçalves, Hugo David, Liliana Matos, Olga Amaral, Luísa Diogo, Sara Ferreira, Constança Santos, Esmeralda Martins, Maria João Prata, Luís Pereira de Almeida, Sandra Alves, Maria Francisca Coutinho
Among the many lysosomal storage disorders (LSDs) that would benefit from the establishment of novel cell models, either patient-derived or genetically engineered, is mucopolysaccharidosis type II (MPS II). Here, we present our results on the establishment and characterization of two MPS II patient-derived stem cell line(s) from deciduous baby teeth. To the best of our knowledge, this is the first time a stem cell population has been isolated from LSD patient samples obtained from the dental pulp. Taking into account our results on the molecular and biochemical characterization of those cells and the fact that they exhibit visible and measurable disease phenotypes, we consider these cells may qualify as a valuable disease model, which may be useful for both pathophysiological assessments and in vitro screenings...
March 21, 2024: International Journal of Molecular Sciences
https://read.qxmd.com/read/38542524/development-and-evaluation-of-an-in-house-real-time-rt-pcr-targeting-nsp10-gene-for-sars-cov-2-detection
#13
JOURNAL ARTICLE
Cyril Chik-Yan Yip, Jane Hau-Ching Poon, Kit-Hang Leung, Wan-Mui Chan, Jonathan Daniel Ip, Allen Wing-Ho Chu, Vincent Chi-Chung Cheng, Kwok-Yung Yuen, Kelvin Kai-Wang To
The emergence of SARS-CoV-2 mutations poses significant challenges to diagnostic tests, as these mutations can reduce the sensitivity of commonly used RT-PCR assays. Therefore, there is a need to design diagnostic assays with multiple targets to enhance sensitivity. In this study, we identified a novel diagnostic target, the nsp10 gene, using nanopore sequencing. Firstly, we determined the analytical sensitivity and specificity of our COVID-19-nsp10 assay. The COVID-19-nsp10 assay had a limit of detection of 74 copies/mL (95% confidence interval: 48-299 copies/mL) and did not show cross-reactivity with other respiratory viruses...
March 21, 2024: International Journal of Molecular Sciences
https://read.qxmd.com/read/38542523/evolution-of-the-wrky-family-in-angiosperms-and-functional-diversity-under-environmental-stress
#14
JOURNAL ARTICLE
Weihuang Wu, Jinchang Yang, Niu Yu, Rongsheng Li, Zaixiang Yuan, Jisen Shi, Jinhui Chen
The transcription factor is an essential factor for regulating the responses of plants to external stimuli. The WRKY protein is a superfamily of plant transcription factors involved in response to various stresses (e.g., cold, heat, salt, drought, ions, pathogens, and insects). During angiosperm evolution, the number and function of WRKY transcription factors constantly change. After suffering from long-term environmental battering, plants of different evolutionary statuses ultimately retained different numbers of WRKY family members...
March 21, 2024: International Journal of Molecular Sciences
https://read.qxmd.com/read/38542522/have-all-of-the-phytohormonal-properties-of-melatonin-been-verified
#15
REVIEW
Woong June Park
Melatonin is a ubiquitous regulator in plants and performs a variety of physiological roles, including resistance to abiotic stress, regulation of growth and development, and enhancement of plant immunity. Melatonin exhibits the characteristics of a phytohormone with its pleiotropic effects, biosynthesis, conjugation, catabolism, effective concentration, and the shape and location of its dose-response curves. In addition, CAND2/PMTR1, a phytomelatonin receptor candidate belonging to the G protein-coupled receptors (GPCRs), supports the concept of melatonin as a phytohormone...
March 21, 2024: International Journal of Molecular Sciences
https://read.qxmd.com/read/38542521/expression-of-e-cadherin-and-n-cadherin-in-the-endocervix-as-a-predictive-factor-in-patients-with-endometrial-cancer
#16
JOURNAL ARTICLE
Karolina Frąszczak, Bartłomiej Barczyński, Bożydar Tylus, Wiesława Bednarek
Endometrial cancer (EC) is the most common gynecological malignancy. This study aimed to evaluate the expression of E-cadherin and N-cadherin in primary endometrial lesions and the endocervix in patients with EC to identify noninvasive predictive factors. In this single-center retrospective study, data on 101 patients who underwent surgery for EC were collected. The immunohistochemical expression of E-cadherin and N-cadherin was assessed depending on the tumor grade, location, and cell differentiation. Correlations between E-cadherin and N-cadherin levels in the endocervix and the primary tumor were determined...
March 21, 2024: International Journal of Molecular Sciences
https://read.qxmd.com/read/38542520/association-of-blast-exposure-in-military-breaching-with-intestinal-permeability-blood-biomarkers-associated-with-leaky-gut
#17
JOURNAL ARTICLE
Qingkun Liu, Zhaoyu Wang, Shengnan Sun, Jeffrey Nemes, Lisa A Brenner, Andrew Hoisington, Maciej Skotak, Christina R LaValle, Yongchao Ge, Walter Carr, Fatemeh Haghighi
Injuries and subclinical effects from exposure to blasts are of significant concern in military operational settings, including tactical training, and are associated with self-reported concussion-like symptomology and physiological changes such as increased intestinal permeability (IP), which was investigated in this study. Time-series gene expression and IP biomarker data were generated from "breachers" exposed to controlled, low-level explosive blast during training. Samples from 30 male participants at pre-, post-, and follow-up blast exposure the next day were assayed via RNA-seq and ELISA...
March 21, 2024: International Journal of Molecular Sciences
https://read.qxmd.com/read/38542519/special-issue-covid-19-coagulopathy-advances-on-pathophysiology-and-therapies
#18
EDITORIAL
Eliza Russu, Emil-Marian Arbănaşi, Alexandru Șchiopu
The Special Issue on COVID-19 coagulopathy initiated one year ago aimed to shed light on the mechanisms underlying the changes in the coagulation status making SARS-CoV-2 infection such a tough adversary for every one of the medical specialties encountering it, along with overseeing the therapeutic applications derived from the current understanding of these mechanisms [...].
March 21, 2024: International Journal of Molecular Sciences
https://read.qxmd.com/read/38542518/ppr596-is-required-for-nad2-intron-splicing-and-complex-i-biogenesis-in-arabidopsis
#19
JOURNAL ARTICLE
Aqib Sayyed, Baoyin Chen, Yong Wang, Shi-Kai Cao, Bao-Cai Tan
Mitochondria are essential organelles that generate energy via oxidative phosphorylation. Plant mitochondrial genome encodes some of the respiratory complex subunits, and these transcripts require accurate processing, including C-to-U RNA editing and intron splicing. Pentatricopeptide repeats (PPR) proteins are involved in various organellar RNA processing events. PPR596, a P-type PPR protein, was previously identified to function in the C-to-U editing of mitochondrial rps3 transcripts in Arabidopsis. Here, we demonstrate that PPR596 functions in the cis-splicing of nad2 intron 3 in mitochondria...
March 21, 2024: International Journal of Molecular Sciences
https://read.qxmd.com/read/38542517/metabolomics-of-plasma-in-xlh-patients-with-arterial-hypertension-new-insights-into-the-underlying-mechanisms
#20
JOURNAL ARTICLE
Luis Carlos López-Romero, José Jesús Broseta, Marta Roca-Marugán, Juan R Muñoz-Castañeda, Agustín Lahoz, Julio Hernández-Jaras
X-linked hypophosphatemia (XLH) is a rare genetic disorder that increases fibroblast growth factor 23 (FGF23). XLH patients have an elevated risk of early-onset hypertension. The precise factors contributing to hypertension in XLH patients have yet to be identified. A multicenter cross-sectional study of adult patients diagnosed with XLH. Metabolomic analysis was performed using ultra-performance liquid chromatography (UPLC) coupled to a high-resolution mass spectrometer. Twenty subjects were included, of which nine (45%) had hypertension...
March 21, 2024: International Journal of Molecular Sciences
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