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Journals International Journal of Molec...

International Journal of Molecular Sciences

https://read.qxmd.com/read/38612943/boosting-clear-cell-renal-carcinoma-specific-drug-discovery-using-a-deep-learning-algorithm-and-single-cell-analysis
#1
JOURNAL ARTICLE
Yishu Wang, Xiaomin Chen, Ningjun Tang, Mengyao Guo, Dongmei Ai
Clear cell renal carcinoma (ccRCC), the most common subtype of renal cell carcinoma, has the high heterogeneity of a highly complex tumor microenvironment. Existing clinical intervention strategies, such as target therapy and immunotherapy, have failed to achieve good therapeutic effects. In this article, single-cell transcriptome sequencing (scRNA-seq) data from six patients downloaded from the GEO database were adopted to describe the tumor microenvironment (TME) of ccRCC, including its T cells, tumor-associated macrophages (TAMs), endothelial cells (ECs), and cancer-associated fibroblasts (CAFs)...
April 8, 2024: International Journal of Molecular Sciences
https://read.qxmd.com/read/38612942/inhibition-of-the-sodium-calcium-exchanger-reverse-mode-activity-reduces-alcohol-consumption-in-rats
#2
JOURNAL ARTICLE
Gleice Kelli Silva-Cardoso, Prosper N'Gouemo
Excessive and uncontrolled consumption of alcohol can cause alcohol use disorder (AUD), but its pharmacological mechanisms are not fully understood. Inhibiting the reverse mode activity of the sodium-calcium exchanger (NCX) can reduce the risk of alcohol withdrawal seizures, suggesting that NCX could play a role in controlling alcohol consumption. Here, we investigated how two potent inhibitors of NCX reverse mode activity, SN-6 (NCX1) and KB-R7943 (NCX3), affect voluntary alcohol consumption in adult male and female rats using the intermittent alcohol access two-bottle choice paradigm...
April 8, 2024: International Journal of Molecular Sciences
https://read.qxmd.com/read/38612941/molecular-research-on-plasmodium-infection-and-immunity
#3
EDITORIAL
Jean-Paul Coutelier, Sylviane Pied
The WHO's global strategy for malaria targets a reduction of at least 90% of both incidence and mortality rates for 2030 [...].
April 8, 2024: International Journal of Molecular Sciences
https://read.qxmd.com/read/38612940/biomolecular-condensates-decipher-molecular-codes-of-cell-fate-from-biophysical-fundamentals-to-therapeutic-practices
#4
REVIEW
Xing Sun, Yangyang Zhou, Zhiyan Wang, Menglan Peng, Xianhua Wei, Yifang Xie, Chengcai Wen, Jing Liu, Mao Ye
Cell fate is precisely modulated by complex but well-tuned molecular signaling networks, whose spatial and temporal dysregulation commonly leads to hazardous diseases. Biomolecular condensates (BCs), as a newly emerging type of biophysical assemblies, decipher the molecular codes bridging molecular behaviors, signaling axes, and clinical prognosis. Particularly, physical traits of BCs play an important role; however, a panoramic view from this perspective toward clinical practices remains lacking. In this review, we describe the most typical five physical traits of BCs, and comprehensively summarize their roles in molecular signaling axes and corresponding major determinants...
April 8, 2024: International Journal of Molecular Sciences
https://read.qxmd.com/read/38612939/a-pilot-analysis-of-whole-transcriptome-of-human-cryopreserved-sperm
#5
JOURNAL ARTICLE
Sara Stigliani, Adriana Amaro, Francesco Reggiani, Elena Maccarini, Claudia Massarotti, Matteo Lambertini, Paola Anserini, Paola Scaruffi
Sperm cryopreservation is a procedure widely used to store gametes for later use, to preserve fertility in patients prior to gonadotoxic treatments or surgery, and for sperm donation programs. The purpose of the study was to assess the impact of cryopreservation on human sperm transcriptome. Semen samples were collected from 13 normospermic men. Each sample was divided into two aliquots. The total RNA was immediately extracted from one aliquot. The second aliquot was frozen and total RNA was extracted after a week of storage in liquid nitrogen...
April 8, 2024: International Journal of Molecular Sciences
https://read.qxmd.com/read/38612938/estrogen-%C3%AE-and-%C3%AE-receptor-expression-in-the-various-regions-of-resected-glioblastoma-multiforme-tumors-and-in-an-in-vitro-model
#6
JOURNAL ARTICLE
Donata Simińska, Klaudyna Kojder, Dariusz Jeżewski, Maciej Tarnowski, Patrycja Tomasiak, Katarzyna Piotrowska, Agnieszka Kolasa, Kapczuk Patrycja, Dariusz Chlubek, Irena Baranowska-Bosiacka
Glioblastoma multiforme (GBM) is a malignant tumor with a higher prevalence in men and a higher survival rate in transmenopausal women. It exhibits distinct areas influenced by changing environmental conditions. This study examines how these areas differ in the levels of estrogen receptors (ERs) which play an important role in the development and progression of many cancers, and whose expression levels are often correlated with patient survival. This study utilized two research models: an in vitro model employing the U87 cell line and a second model involving tumors resected from patients (including tumor core, enhancing tumor region, and peritumoral area)...
April 8, 2024: International Journal of Molecular Sciences
https://read.qxmd.com/read/38612937/kaempferol-as-an-alternative-cryosupplement-for-bovine-spermatozoa-cytoprotective-and-membrane-stabilizing-effects
#7
JOURNAL ARTICLE
Štefan Baňas, Eva Tvrdá, Filip Benko, Michal Ďuračka, Natália Čmiková, Norbert Lukáč, Miroslava Kačániová
Kaempferol (KAE) is a natural flavonoid with powerful reactive oxygen species (ROS) scavenging properties and beneficial effects on ex vivo sperm functionality. In this paper, we studied the ability of KAE to prevent or ameliorate structural, functional or oxidative damage to frozen-thawed bovine spermatozoa. The analysis focused on conventional sperm quality characteristics prior to or following thermoresistance tests, namely the oxidative profile of semen alongside sperm capacitation patterns, and the levels of key proteins involved in capacitation signaling...
April 8, 2024: International Journal of Molecular Sciences
https://read.qxmd.com/read/38612936/insights-on-platelet-derived-growth-factor-receptor-%C3%AE-positive-interstitial-cells-in-the-male-reproductive-tract
#8
REVIEW
Tasuku Hiroshige, Kei-Ichiro Uemura, Kei-Ichiro Nakamura, Tsukasa Igawa
Male infertility is a significant factor in approximately half of all infertility cases and is marked by a decreased sperm count and motility. A decreased sperm count is caused by not only a decreased production of sperm but also decreased numbers successfully passing through the male reproductive tract. Smooth muscle movement may play an important role in sperm transport in the male reproductive tract; thus, understanding the mechanism of this movement is necessary to elucidate the cause of sperm transport disorder...
April 8, 2024: International Journal of Molecular Sciences
https://read.qxmd.com/read/38612935/soluble-nkg2dls-are-elevated-in-breast-cancer-patients-and-associate-with-disease-outcome
#9
JOURNAL ARTICLE
Anna Seller, Christian M Tegeler, Jonas Mauermann, Tatjana Schreiber, Ilona Hagelstein, Kai Liebel, André Koch, Jonas S Heitmann, Sarah M Greiner, Clara Hayn, Dominik Dannehl, Tobias Engler, Andreas D Hartkopf, Markus Hahn, Sara Y Brucker, Helmut R Salih, Melanie Märklin
Ligands of the natural killer group 2D (NKG2DL) family are expressed on malignant cells and are usually absent from healthy tissues. Recognition of NKG2DLs such as MICA/B and ULBP1-3 by the activating immunoreceptor NKG2D, expressed by NK and cytotoxic T cells, stimulates anti-tumor immunity in breast cancer. Upregulation of membrane-bound NKG2DLs in breast cancer has been demonstrated by immunohistochemistry. Tumor cells release NKG2DLs via proteolytic cleavage as soluble (s)NKG2DLs, which allows for effective immune escape and is associated with poor prognosis...
April 8, 2024: International Journal of Molecular Sciences
https://read.qxmd.com/read/38612934/energy-transfer-and-radical-pair-dynamics-in-photosystem-i-with-different-red-chlorophyll-a-pigments
#10
JOURNAL ARTICLE
Ivo H M van Stokkum, Marc G Müller, Alfred R Holzwarth
We establish a general kinetic scheme for the energy transfer and radical-pair dynamics in photosystem I (PSI) of Chlamydomonas reinhardtii , Synechocystis PCC6803, Thermosynechococcus elongatus and Spirulina platensis grown under white-light conditions. With the help of simultaneous target analysis of transient-absorption data sets measured with two selective excitations, we resolved the spectral and kinetic properties of the different species present in PSI. WL-PSI can be described as a Bulk Chl a in equilibrium with a higher-energy Chl a, one or two Red Chl a and a reaction-center compartment (WL-RC)...
April 8, 2024: International Journal of Molecular Sciences
https://read.qxmd.com/read/38612933/effect-of-addition-of-tannin-extract-from-underutilized-resources-on-allergenic-proteins-color-and-textural-properties-of-egg-white-gels
#11
JOURNAL ARTICLE
Yoko Tsurunaga, Mika Ishigaki, Tetsuya Takahashi, Shiori Arima, Sae Kumagai, Yoshimasa Tsujii, Shota Koyama
Tannins, present in numerous plants, exhibit a binding affinity for proteins. In this study, we aimed to exploit this property to reduce the concentration of allergenic egg white proteins. Tannins were extracted, using hot water, from the lyophilized powder of underutilized resources, such as chestnut inner skin (CIS), young persimmon fruit (YPF), and bayberry leaves (BBLs). These extracts were then incorporated into an egg white solution (EWS) to generate an egg white gel (EWG). Allergen reduction efficacy was assessed using electrophoresis and ELISA...
April 8, 2024: International Journal of Molecular Sciences
https://read.qxmd.com/read/38612932/usefulness-and-limitations-of-pfge-diagnosis-and-nucleotide-sequencing-method-in-the-analysis-of-food-poisoning-pathogens-found-in-cooking-employees
#12
JOURNAL ARTICLE
Mi-Na Park, Sang-Gu Yeo, Junhyuk Park, Yoomi Jung, Se-Min Hwang
In the case of a food poisoning outbreak, it is essential to understand the relationship between cooking workers and food poisoning. Many biological diagnostic methods have recently been developed to detect food poisoning pathogens. Among these diagnostic tools, this study presents PCR-based pulsed-field gel electrophoresis and nucleotide sequencing diagnostic analysis results for diagnosing food poisoning outbreaks associated with cooking employees in Chungcheongnam-do, Republic of Korea. Pulsed-field gel electrophoresis was useful in identifying the food poisoning outbreaks caused by Staphylococcus aureus and Enteropathogenic Escherichia coli...
April 8, 2024: International Journal of Molecular Sciences
https://read.qxmd.com/read/38612931/the-anti-microbial-peptide-citrocin-controls-pseudomonas-aeruginosa-biofilms-by-breaking-down-extracellular-polysaccharide
#13
JOURNAL ARTICLE
Liyao Wang, Jiaqi Zheng, Wenchao Hou, Chaowen Zhang, Jie Zhang, Xuanbo Fan, Hongliang Zhang, Yuzhu Han
Citrocin is an anti-microbial peptide that holds great potential in animal feed. This study evaluates the anti-microbial and anti-biofilm properties of Citrocin and explores the mechanism of action of Citrocin on the biofilm of P. aeruginosa . The results showed that Citrocin had a significant inhibitory effect on the growth of P. aeruginosa with a minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of 0.3 mg/mL. All five concentrations (1/4MIC, 1/2MIC, MIC, 2MIC, and 4MIC) of Citrocin inhibited P...
April 8, 2024: International Journal of Molecular Sciences
https://read.qxmd.com/read/38612930/elucidating-the-role-of-oxphos-variants-in-asthenozoospermia-insights-from-whole-genome-sequencing-and-an-in-silico-analysis
#14
JOURNAL ARTICLE
Maria-Anna Kyrgiafini, Themistoklis Giannoulis, Alexia Chatziparasidou, Zissis Mamuris
Infertility is a global health challenge that affects an estimated 72.4 million people worldwide. Between 30 and 50% of these cases involve male factors, showcasing the complex nature of male infertility, which can be attributed to both environmental and genetic determinants. Asthenozoospermia, a condition characterized by reduced sperm motility, stands out as a significant contributor to male infertility. This study explores the involvement of the mitochondrial oxidative phosphorylation (OXPHOS) system, crucial for ATP production and sperm motility, in asthenozoospermia...
April 8, 2024: International Journal of Molecular Sciences
https://read.qxmd.com/read/38612929/the-golgi-apparatus-a-key-player-in-innate-immunity
#15
REVIEW
Ion Mărunţelu, Alexandra-Elena Constantinescu, Razvan-Adrian Covache-Busuioc, Ileana Constantinescu
The Golgi apparatus, long recognized for its roles in protein processing and vesicular trafficking, has recently been identified as a crucial contributor to innate immune signaling pathways. This review discusses our expanding understanding of the Golgi apparatus's involvement in initiating and activating these pathways. It highlights the significance of membrane connections between the Golgi and other organelles, such as the endoplasmic reticulum, mitochondria, endosomes, and autophagosomes. These connections are vital for the efficient transmission of innate immune signals and the activation of effector responses...
April 8, 2024: International Journal of Molecular Sciences
https://read.qxmd.com/read/38612928/the-effect-of-composition-on-the-properties-and-application-of-cuo-nio-nanocomposites-synthesized-using-a-saponin-green-microwave-assisted-hydrothermal-method
#16
JOURNAL ARTICLE
Amnah Al-Yunus, Wafa Al-Arjan, Hassan Traboulsi, Manal Hessien
In this study, we explored the formation of CuO nanoparticles, NiO nanoflakes, and CuO-NiO nanocomposites using saponin extract and a microwave-assisted hydrothermal method. Five green synthetic samples were prepared using aqueous saponin extract and a microwave-assisted hydrothermal procedure at 200 °C for 30 min. The samples were pristine copper oxide (100C), 75% copper oxide-25% nickel oxide (75C25N), 50% copper oxide-50% nickel oxide (50C50N), 25% copper oxide-75% nickel oxide (25C75N), and pristine nickel oxide (100N)...
April 8, 2024: International Journal of Molecular Sciences
https://read.qxmd.com/read/38612927/extracellular-vesicles-as-surrogates-for-the-regulation-of-the-drug-transporters-abcc2-mrp2-and-abcg2-bcrp
#17
JOURNAL ARTICLE
Juan Pablo Rigalli, Anna Gagliardi, Klara Diester, Gzona Bajraktari-Sylejmani, Antje Blank, Jürgen Burhenne, Alexander Lenard, Lars Werntz, Andrea Huppertz, Lena Münch, Janica Margrit Wendt, Max Sauter, Walter Emil Haefeli, Johanna Weiss
Drug efflux transporters of the ATP-binding-cassette superfamily play a major role in the availability and concentration of drugs at their site of action. ABCC2 (MRP2) and ABCG2 (BCRP) are among the most important drug transporters that determine the pharmacokinetics of many drugs and whose overexpression is associated with cancer chemoresistance. ABCC2 and ABCG2 expression is frequently altered during treatment, thus influencing efficacy and toxicity. Currently, there are no routine approaches available to closely monitor transporter expression...
April 8, 2024: International Journal of Molecular Sciences
https://read.qxmd.com/read/38612926/tumor-and-peritoneum-associated-macrophage-gene-signature-as-a-novel-molecular-biomarker-in-gastric-cancer
#18
JOURNAL ARTICLE
Kevin M Sullivan, Haiqing Li, Annie Yang, Zhifang Zhang, Ruben R Munoz, Kelly M Mahuron, Yate-Ching Yuan, Isaac Benjamin Paz, Daniel Von Hoff, Haiyong Han, Yuman Fong, Yanghee Woo
A spectrum of immune states resulting from tumor resident macrophages and T-lymphocytes in the solid tumor microenvironment correlates with patient outcomes. We hypothesized that in gastric cancer (GC), macrophages in a polarized immunosuppressive transcriptional state would be prognostic of poor survival. We derived transcriptomic signatures for M2 (M2TS , MRC1 ; MS4A4A ; CD36 ; CCL13 ; CCL18 ; CCL23 ; SLC38A6 ; FGL2 ; FN1 ; MAF ) and M1 (M1TS , CCR7 ; IL2RA ; CXCL11 ; CCL19 ; CXCL10 ; PLA1A ; PTX3 ) macrophages, and cytolytic T-lymphocytes (CTLTS , GZMA ; GZMB ; GZMH ; GZMM ; PRF1 )...
April 8, 2024: International Journal of Molecular Sciences
https://read.qxmd.com/read/38612925/genome-wide-identification-and-analysis-of-the-ein3-eil-transcription-factor-gene-family-in-doubled-haploid-dh-poplar
#19
JOURNAL ARTICLE
Caixia Liu, Erqin Fan, Yuhang Liu, Meng Wang, Qiuyu Wang, Sui Wang, Su Chen, Chuanping Yang, Xiangling You, Guanzheng Qu
Ethylene (ET) is an important phytohormone that regulates plant growth, development and stress responses. The ethylene-insensitive3/ethylene-insensitive3-like (EIN3/EIL) transcription factor family, as a key regulator of the ET signal transduction pathway, plays an important role in regulating the expression of ET-responsive genes. Although studies of EIN3/EIL family members have been completed in many species, their role in doubled haploid (DH) poplar derived from another culture of diploid Populus simonii × P...
April 8, 2024: International Journal of Molecular Sciences
https://read.qxmd.com/read/38612924/the-oligomeric-state-of-vasorin-in-the-plasma-membrane-measured-non-invasively-by-quantitative-fluorescence-fluctuation-spectroscopy
#20
JOURNAL ARTICLE
Junyi Liang, Adam W Smith
Vasorin (VASN), a transmembrane protein heavily expressed in endothelial cells, has garnered recent interest due to its key role in vascular development and pathology. The oligomeric state of VASN is a crucial piece of knowledge given that receptor clustering is a frequent regulatory mechanism in downstream signaling activation and amplification. However, documentation of VASN oligomerization is currently absent. In this brief report, we describe the measurement of VASN oligomerization in its native membranous environment, leveraging a class of fluorescence fluctuation spectroscopy...
April 8, 2024: International Journal of Molecular Sciences
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