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Enhancing Chromatographic Analysis: A Novel Cryo-Enrichment Module for GC Analysis of Terpenes in Cannabis sativa.
Journal of Chromatographic Science 2023 September 24
This study presents the development and validation of a gas chromatography (GC) method using cryo-enrichment for the analysis of α-pinene and β-caryophyllene in Cannabis sativa plant. Cryo-enrichment is a method that involves cooling part of the GC column to improve some aspect of chromatographic analysis. The aim of this study was to improve upon previous GC cryo-enrichment prototype and to create and validate a method for the analysis of these terpene compounds, which have received increasing attention for their medicinal properties. The improved cryo-enrichment device used in this study was built in-house and used a custom-built aluminum GC column holder cooled by a two-stage cryo-cooler. The validated method was found to be precise, accurate and sensitive, with good repeatability.
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