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Journal of Chromatographic Science

Zeynab Tavakoli, Majid Soleimani, Mir Mohammad Alavi Nikje
Industrial polyurethane rigid foam (PUF) was selected as a substrate for selective solid phase extraction of Alprazolam. Effective parameters for raising selectivity of the PUF were evaluated. Synthetic molecularly imprinted polyurethane foam (MIPUF) was tracked as selective adsorbent and its characteristic was pondered by analytical methods. Optimization was done by central composite design (CCD) to have high efficiency of the polymer adsorption. Two different extraction methods were compared in the selective adsorption processes using MIPUF and NIPUF, batch system and continuous (cartridge) system...
April 20, 2019: Journal of Chromatographic Science
Eman Saad Elzanfaly, Enas Abd Elhakim Amer, Sara Abd Elbasset Galal, Hala Elsayed Zaazaa
Eptifibatide (EPT) is a cyclic heptapeptide derived from a protein found in the venom of the south-eastern pygmy rattle snake used as an antiplatelet drug. In this study, a newly developed HPLC method demonstrating no interference from the different degradation products of EPT has been optimized and validated. The method was based on HPLC separation of eptifibatide from its degradation products using reversed phase C18 column at ambient temperature with mobile phase consisting of acetonitrile: 50 mM sodium dihydrogen orthophosphate dihydrate, pH was adjusted to 2...
April 20, 2019: Journal of Chromatographic Science
Sara da Silva Anacleto, Hanna Leijoto de Oliveira, Anny Talita Maria da Silva, Tienne Aparecida do Nascimento, Marcella Matos Cordeiro Borges, Ricky Cássio Dos Santos Silva, Arnaldo César Pereira, Keyller Bastos Borges
Herein, an organic-inorganic hybrid molecularly imprinted polymer (MIP) was successfully synthesized with albendazole sulfoxide (ABZSO) as a template and 3-(trimethoxysilyl)propyl methacrylate, a bifunctional group compound, as a single cross-linking agent. In this study, a simple method using HPLC-DAD was developed for the determination of ABZSO enantiomers in human urine using pipette tip-based molecularly imprinted polymer solid phase extraction (PT-MIP-SPE). Enantioseparation with satisfactory retention times (5...
April 20, 2019: Journal of Chromatographic Science
Yi Tao, Xiaoping Zhou, Weidong Li, Baochang Cai
Fallopia multiflora is used for treatment of premature graying hair and blood deficiency. In this study, a quantitative method was developed for determination of five bioactive components (emodin, 2,3,5,4'-tetrahydroxy-stilbene- 2-Ο-β-d-glucoside, emodin-8-O-β-d-glucopyranoside, ω-hydroxyemodin and kaempferol) in raw and processed F. multiflora by using ultra-high performance liquid chromatography (UHPLC)-quadrupole time-of-flight mass spectrometry-based method. The sample handling procedure was optimized...
April 17, 2019: Journal of Chromatographic Science
Marwa A A Ragab, Mohamed H Abdel-Hay, Hytham M Ahmed, Sara M Mohyeldin
Two chromatographic methods (high performance thin layer chromatography (HPTLC) and high performance liquid chromatography-diode array detector (HPLC-DAD)), were addressed for the analysis of a mixture consisted of phenylephrine hydrochloride and ibuprofen in two forms bulk and their combined dosage form. This binary mixture is considered to be a challenging one as the two drugs differ greatly in their chemical and physical properties. Not only this affects their simultaneous analysis, but also hinders their simultaneous extraction from biological fluids as plasma...
April 17, 2019: Journal of Chromatographic Science
Shereen A Boltia, Aya T Soudi, Eman S Elzanfaly, Hala E Zaazaa
Two sensitive chromatographic methods have been developed, and validated for chlorpheniramine maleate (CM), phenylephrine (PE) and guaifenesin (GF) determination in their mixture and in presence of GF related substance guaiacol (GL) and preservative namely; sodium benzoate (NaB). The first method was based on thin layer chromatographic separation (TLC) followed by densitometric determination of the separated spots. The separation was achieved using silica gel 60 F254 TLC plates and ethyl acetate: methanol: toluene: ammonia (7:1...
April 17, 2019: Journal of Chromatographic Science
Clayton B'Hymer
The analysis of degradation products from the classic chemical warfare nerve agents by high-performance liquid chromatography-mass spectrometry has been of much interest in recent years owing to the possible use as a terrorist weapon, and the incidents of chemical weapon usage in recent years in war torn countries. The alkyl methylphosphonic acid degradation products are of a particular interest, and they represent a specific chromatographic technical challenge for use in typical separation systems. Various published methods are summarized in this review and some of the problems associated with the analysis of these compounds are discussed...
April 17, 2019: Journal of Chromatographic Science
Fawzia Ibrahim, Mohie K Sharaf El-Din, Asmaa Kamal El-Deen, Kuniyoshi Shimizu
A new simple and robust HPLC-DAD method was developed for the concurrent analysis of hydroquinone (HQ), hydrocortisone acetate (HCA) and tretinoin (TRN) triple combination for the first time using an Inertsil ODS 3-C18 column (150 mm × 4.6 mm, 5 μm particle size) column with 0.05 M phosphate buffer (pH 5.0) and acetonitrile at a ratio of (10:90, v/v) as a mobile phase, eluted by an isocratic elution mode at a flow rate of 1.0 mL/min and detected at 265 nm. Mefenamic acid was used as an internal standard (I...
April 3, 2019: Journal of Chromatographic Science
Poonam Malik, Ravi Bhushan
Direct resolution of (RS)-ketorolac and (RS)-etodolac has been achieved by ligand exchange thin layer chromatography. Cu(II) has been used as a complexing ion with three enantiomerically pure amino acids (namely, l-tryptophan, l-histidine and l-phenylalanine) as chiral dopants. Chromatograms were developed using different combinations of solvent systems in different ratio having no chiral additive. Iodine vapors were used for location of spots. Different experimental factors, i.e., effect of temperature, mole ratio of Cu(II) to l-amino acids and solvent ratio were optimized in order to improve the separation efficiency...
April 3, 2019: Journal of Chromatographic Science
Shereen Mostafa Tawakkol, Yasmin Mohammed Fayez, Nesma Mahmoud Fahmy, Hayam Mahmoud Lotfy, Mostafa Abdel-Atty Shehata
Two stability-indicating chromatographic methods for the determination of clotrimazole and its two acid induced degradation products, with dexamethasone acetate without prior separation. First method depends on RP-HPLC utilizing ODS-3 Inertsil C18 column. Mobile phase consists of acetonitrile:phosphate buffer (pH 6.0) in ratio (65:35, v/v) with flow rate 1.5 mL/min and UV-detection at 220 nm. Linearity range 1.0-75.0 μg/mL for clotrimazole and 2.0-75.0 μg/mL for dexamethasone with mean percentage recovery of 99...
March 31, 2019: Journal of Chromatographic Science
Yubo Wang, Qianqian Xu, Zhiqiang Shen, Yanping Wang, Lin Dong, Shijun Fu, Limei Yang, Mimi Qin, Ying Zhang, Shijin Guo
Baitouweng Decotion (BD) is a famous traditional Chinese medicinal prescription, which is composed of Pulsatilla chinensis (bunge) regel, Coptis chinensis franch., Phellodendron chinense and Cortex Fraxini. In this study, a simple and sensitive high performance liquid chromatography coupled with ultraviolet detection method was established for the simultaneous determination of eight marker compounds including Esculin, Fraxin, Esculetin, Fraxetin, Columbamine, Coptisine, Palmatine Chloride and Berberine hydrochloride in BD, the single herbs and their negative controls...
March 31, 2019: Journal of Chromatographic Science
Mwila Mulubwa, Pierre Mugabo
A simple and sensitive ultra-performance liquid chromatography tandem mass spectrometry method has been developed and validated for the analysis of cycloserine in patients' plasma. Using methanol, cyloserine and propranolol (internal standard (IS)) was extracted from plasma by protein precipitation procedure. The chromatographic separation was successfully achieved on Phenomenex KinetexTM PFP C18 (2.1 mm × 100 mm, 2.6 μm) reversed-phase column. Acidified with 0.1% formic acid, water and acetonitrile were used as mobile phases for gradient elution...
March 30, 2019: Journal of Chromatographic Science
Dongmei Chen, Mengru Wu, Shuyu Xie, Xueqin Li, Yanfei Tao, Xu Wang, Lingli Huang, Yuanhu Pan, Dapeng Peng, Zonghui Yuan
Pigments are still widely used in food and feed industry and their resides in food might be harmful to human health due to their side effects. A high performance liquid chromatography (HPLC) method for simultaneous determination of pigments including tartrazine, lutein, capsanthin, canthaxanthin and β-carotene in animal-derived foods (including the muscle and liver of swine, the muscle, liver and skin of chicken and duck, and the muscle of fish) and feeds (swine, chicken and duck) was developed. Lutein, capsanthin, canthaxanthin and β-carotene were extracted with acetonitrile-ethyl acetate by ultrasonication, and tartrazine was extracted with water, followed by defatting with n-hexane and clean-up by solid phase extraction on weak anion exchange cartridges...
March 30, 2019: Journal of Chromatographic Science
Ana Carolina Kogawa, Ann Van Schepdael, Hérida Regina Nunes Salgado
Rifaximin, an oral antimicrobial, has no standardized analytical methods in many official compendia. In this context, the aim was to develop and validate an analytical method by capillary electrophoresis for quantification of rifaximin in tablets. The choice of capillary electrophoresis was based on the concepts of green analytical chemistry, taking into account the health of the operator and environment, analytical conscience, as well as the parameters of linearity, selectivity, precision, accuracy and robustness...
March 30, 2019: Journal of Chromatographic Science
Abolghasem Beheshti, Solmaz Ghaffari, Hadi Farahani
Nanoliposomes are extensively used as ideal vehicles in drug delivery systems due to their unique biocompatibility and biodegradability properties. They can be used as sustained release and target selective conveyances to deliver the encapsulated drugs at specific cells or tissues by improving their efficacy along with reducing the side effects. As an analytical perspective, the determination of various lipid components in the final formulation is one of the practical issues while the agents are applied in an industrial-scale...
March 30, 2019: Journal of Chromatographic Science
Nadine George, Magy Herz, Hassan Y Aboul-Enein, Lamia Shihata, Rasha Hanafi
A quality-by-design approach was adopted for enantioseparation of atenolol on Vancomycin and Teicoplanin-based chiral stationary phases using reversed phase (RP) mode and polar ionic mode (PIM), respectively to account for major forces involved in enantiorecognition of β-blockers on macrocyclics. A fractional factorial screening design for the two modes; followed by a central composite optimization design and regression analysis were able to point out critical factors and chromatographic responses and robust surface of the design...
March 20, 2019: Journal of Chromatographic Science
Rusen Koray Eyison, Sermet Sezigen, Mesut Ortatatli, Levent Kenar
Sulfur mustard (SM) which is a bifunctional alkylating vesicant is one of the mostly used chemical warfare agent in First World War and the Iran-Iraq War. β-Lyase metabolites of SM especially 1,1'-sulfonylbis[2-(methylthio)ethane] (SBMTE) is an unequivocal biomarker of the exposure. An optimized gas chromatography-tandem mass spectrometry method was developed and validated for the retrospective detection of SBMTE in human urine. Urine samples were treated with acidic titanium trichloride to reduce β-lyase metabolites to the single analyte SBMTE...
March 18, 2019: Journal of Chromatographic Science
Lianlian Zhu, Xiaoning Luan, Deqiang Dou, Luqi Huang
Ginsenosides and oligosaccharides were comparatively analyzed in WG, RG and BG. The HPLC fingerprints of ginsenosides and oligosaccharides were established to unravel the characteristics of ginsenosides and oligosaccharides. The primary ginsenosides (Rg1, Rb1, Re, Rc, Rb2 and Rd, etc.) in WG were higher than those in RG and BG, but the variety of ginsenosides in RG is highest and the non-polar rare saponins are higher than RG. Five kinds of saccharides encompassed fructose, glucose, sucrose, maltose and nystose, respectively, were determined in WG, RG and BG, disclosing that, the sucrose in WG is 1...
March 6, 2019: Journal of Chromatographic Science
Pallab Mandal, Shubhasis Dan, Soumya Chakraborty, Balaram Ghosh, Chiranjit Saha, Jasmina Khanam, Tapan Kumar Pal
Among the secondary metabolites which are widely distributed in plants and foods in plant origin flavonoids is important one. Flavonoids have antioxidant activities as free radical scavenging action. They also have anti-inflammatory, antiulcer and anti-carcinogenic activities. Diosmin and hesperidin, the metabolites of which are diosmetin and hesperitin respectively are considered in the present study. Diosmetin has anticancer, antioxidant and blood lipid lowering activities. It also enhances venous tone and microcirculation and by reducing systemic oxidative stress it protects capillaries...
February 27, 2019: Journal of Chromatographic Science
Priscila Aburachid Cardoso, Diego Beltrão Pereira, Mohammed Farrag El-Behairy, Ashraf Ghanem, Gerson Antônio Pianetti, Isabela Costa César
Chloroquine is a chiral antimalarial drug and demonstrates enantioselective pharmacodynamic and pharmacokinetic properties. However, this drug is administered as racemate. The knowledge of stereoselective aspects of these agents may be useful to better understand their mechanisms of action and to optimize their safety and/or clinical efficacy. In this study, an enantioselective analytical method for the quantification of chloroquine enantiomers was developed using HPLC-UV. The chromatographic conditions were: Chirobiotic V column (100 × 2...
February 23, 2019: Journal of Chromatographic Science
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