We have located links that may give you full text access.
Cloning and analysis of mold-specific genes in the dimorphic fungus Histoplasma capsulatum.
Gene 2001 September 6
A critical feature in the pathogenesis of the respiratory pathogen Histoplasma capsulatum is the conversion from the mold form (found in soil) to the yeast form in the lungs of the host. Little is known about the molecular biology of Histoplasma dimorphism. In particular, the possible roles of genes which are transcriptionally silent in yeast (i.e. mold-specific) have not been studied. We have produced a cDNA library highly enriched for mold-upregulated clones by fragmenting cDNA and removing yeast-specific and common sequences with a highly efficient enzyme degrading subtraction method. Screening of randomly selected clones identified cDNA fragments representing 16 different mold-upregulated genes. Because multiple cDNA fragments can be treated as alleles in a genetic screen, we were able to apply probability analysis to estimate the total number of mold-upregulated genes. We estimate that there are 27 upregulated genes; cDNA fragments of 16 have been isolated. Here we report the first isolation and analysis of cDNA from two mold-specific genes, MS8 (GenBank AF292398) and MS88 (GenBank AF357882). The MS8 transcript was very strongly expressed in mold but not detected on Northern blots with yeast RNA. The putative MS8 protein was predicted to be 21.3 kDa (203 aa), very rich in glutamine and glycine and had a calculated pI of 6.76. The MS88 transcript was weakly expressed in mold and not detected in yeast. The putative MS88 protein was predicted to be 22.5 kDa (219 aa) with a pI of 4.46. GenBank similarity searches revealed that the putative MS8 protein was similar to a glutamine-rich protein, of unknown function, from the fungus Colletotrichum gloeosporioides (GenBank U94186). No significant matches were found for the putative MS88 protein.
Full text links
Related Resources
Trending Papers
Interstitial Lung Disease: A Review.JAMA 2024 April 23
Review article: Recent advances in ascites and acute kidney injury management in cirrhosis.Alimentary Pharmacology & Therapeutics 2024 March 26
Executive Summary: State-of-the-Art Review: Unintended Consequences: Risk of Opportunistic Infections Associated with Long-term Glucocorticoid Therapies in Adults.Clinical Infectious Diseases 2024 April 11
Clinical practice guidelines on the management of status epilepticus in adults: A systematic review.Epilepsia 2024 April 13
Get seemless 1-tap access through your institution/university
For the best experience, use the Read mobile app
All material on this website is protected by copyright, Copyright © 1994-2024 by WebMD LLC.
This website also contains material copyrighted by 3rd parties.
By using this service, you agree to our terms of use and privacy policy.
Your Privacy Choices
You can now claim free CME credits for this literature searchClaim now
Get seemless 1-tap access through your institution/university
For the best experience, use the Read mobile app