journal
https://read.qxmd.com/read/38759739/delving-a-stochastic-seqaijr-covid-19-model-with-hexa-delayed-and-copious-control-strategies
#1
JOURNAL ARTICLE
M Pitchaimani, U Aswini
The actual ideal of the literature is to probe the effect of external undulation on prevalence of COVID-19. Owing to some environmental influences, we enhanced our model to the stochastic perturbations. In this article, we scrutinize a stochastic epidemic COVID-19 model with seven states of epidemiological classification. Incipiently, a sophisticated biological system with the effect of time lags put forward copious strategies. At the outset, aim of this work is to explore the existence and uniqueness of global positive solution...
May 15, 2024: Gene
https://read.qxmd.com/read/38759738/circcul3-drives-malignant-progression-of-cervical-cancer-by-activating-autophagy-through-sponge-mir-223-3p-upregulation-of-atg7
#2
JOURNAL ARTICLE
Jiahui Qin, Yan Chen, Xia Zhao, Jingmin Yu
Circular RNA (circRNA) has emerged as a pivotal regulatory factor in cancer biology, yet its exact role in cervical cancer remains incompletely understood. In this study, we investigated the functional role of circCUL3 in cervical cancer and explored its potential as a therapeutic target. Functional gain and loss experiments were conducted in Hela and Siha cell lines to elucidate the biological functions of circCUL3 in cervical cancer. The results revealed that circCUL3 overexpression significantly enhanced cell viability, migration, and invasion while suppressing apoptosis, while circCUL3 knockout displayed the opposite effects...
May 15, 2024: Gene
https://read.qxmd.com/read/38759737/exploring-the-binding-affinity-and-characteristics-of-dcitobp9-in-citrus-psyllids
#3
JOURNAL ARTICLE
Haoran Pei, Gang Xie, Xiang Yao, Shenghui Wang, Jin Yan, Liangying Dai, Yunsheng Wang
Odorant-binding proteins (OBPs) are crucial in insect olfaction. The most abundant expressed OBP of citrus psyllids, DcitOBP9 encodes 148 amino acids. DcitOBP9 lacks a transmembrane structure and possesses a 17-amino acid signal peptide at the N-terminus. Characterized by the six conserved cysteine sites, DcitOBP9 is classified as the Classical-OBP family. RT-qPCR experiments revealed ubiquitous expression of DcitOBP9 across all developmental stages of the citrus psyllid, with predominant expression in adults antennae...
May 15, 2024: Gene
https://read.qxmd.com/read/38754570/the-deletion-of-asb15b-gene-can-lead-to-a-significant-decrease-in-zebrafish-intermuscular-bone
#4
JOURNAL ARTICLE
Minghui Niu, Huamin Whang, Zhichao Wu, ShouwenJiang, Liangbiao Chen
Intercellular bones, which are present in numerous economically significant fish species, have a negative impact on the development of aquaculture. The Asb15b gene, primarily expressed in skeletal muscle, plays a crucial role in regulating protein turnover and the development of muscle fibers. It stimulates protein synthesis and controls the differentiation of muscle fibers. In this study, we employed CRISPR/Cas9 technology to generate homozygous zebrafish strains with 7 bp and 49 bp deletions in the Asb15b gene...
May 14, 2024: Gene
https://read.qxmd.com/read/38754569/investigation-of-crucial-genes-and-mitochondrial-function-impairment-in-diabetic-cardiomyopathy
#5
JOURNAL ARTICLE
Maierhaba Tuersuntuoheti, Lei Zhou, Juexing Li, Shangneng Yang, Suying Zhou, Hui Gong
BACKGROUND: Diabetic cardiomyopathy (DCM) is a special type of cardiovascular disease, termed as a situation of abnormal myocardial structure and function that occurs in diabetic patients. However, the most fundamental mechanisms of DCM have not been fully explicated, and useful targets for the therapeutic strategies still need to be explored. METHODS: In the present study, we combined bioinformatics analysis and in vitro experiments throughout the process of DCM...
May 14, 2024: Gene
https://read.qxmd.com/read/38754568/an-entropy-based-study-on-the-mutational-landscape-of-sars-cov-2-in-usa-comparing-different-variants-and-revealing-co-mutational-behavior-of-proteins
#6
JOURNAL ARTICLE
Daniele Santoni
COVID-19 emergency has pushed the international scientific community to use every resource to combat the spread of the virus, to understand its biology and predict its possible evolution in terms of new variants. Since the first SARS-CoV-2 virus nucleotide and amino acid sequences were made available, information theory was used to study how viral information content was changing over time and then trace the evolution of its mutational landscape. In this work we analyzed SARS-CoV-2 sequences collected mainly in the USA in a period from March 2020 until December 2022 and computed mutation profiles of viral proteins over time through an entropy-based approach using Shannon Entropy and Hellinger distance...
May 14, 2024: Gene
https://read.qxmd.com/read/38754567/generation-of-a-new-mouse-model-for-usher-syndrome-crossing-kunming-mice-with-cba-j-mice
#7
JOURNAL ARTICLE
Shaoheng Li, Yihong Jiang, Lei Zhang, Weiming Yan, Dongyu Wei, Min Zhang, Bin Zhu, Tao Chen, Xiaocheng Wang, Zuoming Zhang, Yuting Su
BACKGROUND: Previously, we discovered a strain of Kunming mice, referred to as the KMush/ush strain, that exhibited notably abnormal electroretinogram (ERG) readings and elevated thresholds for auditory brainstem responses (ABRs), which resembled the characteristics of Usher Syndrome (USH). We successfully identified the pathogenic genes, Pde6b and Adgrv1, after KMush/ush crossbred with CBA/CaJ mice, referred to as CBA-1ush/ush , CBA-2ush/ush or CBA-2ush/ush . In this investigation, we crossbred KMush/ush and CBA/J mice to establish novel recombinant inbred lines and analysed their phenotypic and genotypic characteristics...
May 14, 2024: Gene
https://read.qxmd.com/read/38750802/long-term-effect-of-sars-cov-2-variant-challenging-issues-and-controlling-strategies
#8
JOURNAL ARTICLE
M Pitchaimani, U Aswini
In this study, a latest version of COVID-19 pandemic is hand overed. A Stochastic post COVID-19 delayed model is developed to explore the spread of COVID-19 as well as omicron variant with the correlation of heart attack. This article provides an eradication of the COVID-19 and omicron variant as well as the population who have heart attack after post COVID-19 of these epidemic diseases. Then the existence and uniqueness of global positive solution are studied. Ensuing, In this article, we classify COVID-19 virus and omicron variant which go to extinction and become persistent in mean...
May 13, 2024: Gene
https://read.qxmd.com/read/38740354/investigation-of-diabetes-related-molecular-changes-in-embryo-endometrium-crosstalk
#9
JOURNAL ARTICLE
Semih Tan, Seçil Tan, Onur Tokgün, Hülya Çetin, Elvan Tokgün, Saim Özdamar
The primary aim of this study was to explore the impact of diabetes on matrix metalloproteases and tissue inhibitors, crucial factors for successful implantation, and to elucidate the molecular mechanisms that undergo changes in the endometrium and the embryo during diabetic pregnancies. In this investigation, we established a streptozotocin-induced diabetic pregnant rat model. Microarray analysis followed by RT-PCR was utilized to identify gene regions exhibiting expression alterations. Subsequently, we assessed the effects of MMPs and tissue inhibitors using ELISA and immunohistochemistry techniques, in addition to analyzing changes at the genetic level...
May 11, 2024: Gene
https://read.qxmd.com/read/38740353/identification-and-fine-mapping-of-brmmd1-gene-controlling-recessive-genic-male-sterility-in-brassica-rapa-l
#10
JOURNAL ARTICLE
Xingpeng Xiong, Xiaoyao Li, Shuting Zhang, Zhen Hu, Tingting Liu, Zhengming Qiu, Jiashu Cao, Li Huang, Chenghuan Yan
Recessive genic male sterility (RGMS) provides an effective approach for the commercial exploitation of heterosis, especially in Brassica crops. Although some artificial RGMS mutants have been reported in B. rapa, no causal genes derived from these natural mutants have been identified so far. In this study, a spontaneous RGMS mutant Bcajh97-01A derived from the 'Aijiaohuang' line traced back to the 1980 s was identified. Genetic analysis revealed that the RGMS trait was controlled by a single locus in the Bcajh97-01A/B system...
May 11, 2024: Gene
https://read.qxmd.com/read/38740352/investigate-the-potential-impact-of-hemagglutinin-from-the-h1n1-strain-on-severe-pneumonia
#11
JOURNAL ARTICLE
Yu-Bi Zheng, Song Lu, Tian-Bao Chu, Gui-Feng Pang, Lin-Ying Yang, Qing Zhang
The most prevalent glycoprotein on the influenza virus envelope is called hemagglutinin (HA), yet little is known about its involvement in the pathophysiology and etiology of severe influenza pneumonia. Here, after stimulating human bronchial epithelial cells (16-HBE) and mice with HA of H1N1 for 12 h, we investigated the proliferation, migration, inflammatory cytokines expression, and apoptosis in 16-HBE and the pathological damage in mouse lung tissue. The expression of inflammatory cytokines plasminogen activator inhibitor 1(PAI-1), urokinase-type (uPA) and tissue-type (tPA) plasminogen activators, and apoptosis were all enhanced by HA, which also prevented the proliferation and migration of bronchial epithelial cells...
May 11, 2024: Gene
https://read.qxmd.com/read/38735598/spirulina-versus-metformin-for-controlling-some-insulin-signaling-pathway-genes-in-induced-polycystic-ovary-syndrome-rat-model
#12
JOURNAL ARTICLE
Asmaa A El Leithy, Mohamed Abozaid, Ahmed A Al-Karmalawy, Rasha Mahmoud Allam, Anas Zakarya Nourelden, Rodina M Amer, Nesreen H Abd El Azeem, Faten Sabra Abo-Zeid
Polycystic ovary syndrome (PCOS) is a prevalent endocrinologic and gynecologic disorder that affects women of reproductive age; besides, insulin resistance (IR) occurs in 50-70 % of PCOS cases. Metformin (Met) is commonly prescribed for IR management; however, it does not affect IR with some gastrointestinal symptoms. Spirulina platensis (SP) is a blue-green alga that may increase insulin sensitivity. Therefore, our study aims to evaluate SP as an alternative treatment to Met for improving glucose homeostasis by assessing the expression of 11 crucial genes involved in the insulin signaling pathway...
May 10, 2024: Gene
https://read.qxmd.com/read/38734190/repurposing-of-antimycobacterium-drugs-for-covid-19-treatment-by-targeting-sars-cov-2-main-protease-an-in-silico-perspective
#13
JOURNAL ARTICLE
Ayon Chakraborty, Rajesh Ghosh, Saswati Soumya Mohapatra, Subhashree Barik, Ashis Biswas, Snehasis Chowdhuri
The global mortality rate has been significantly impacted by the COVID-19 pandemic, caused by the SARS CoV-2 virus. Although the pursuit for a potent antiviral is still in progress, experimental therapies based on repurposing of existing drugs is being attempted. One important therapeutic target for COVID-19 is the main protease (Mpro) that cleaves the viral polyprotein in its replication process. Recently minocycline, an antimycobacterium drug, has been successfully implemented for the treatment of COVID-19 patients...
May 9, 2024: Gene
https://read.qxmd.com/read/38734189/role-of-the-ntrc-family-response-regulator-in-nitrogen-metabolism-of-acinetobacter-baumannii
#14
JOURNAL ARTICLE
Ajit Ramesh Sawant, Sudhakar Pagal, K Prashanth
Acinetobacter baumannii is an important Gram-negative nosocomial pathogen that causes opportunistic infections and employs different mechanisms to survive in the presence of antibiotics in the host. Nutrient limitation is one of the important defense mechanisms of the mammalian immune system to fight against the colonization of pathogens like A. baumannii. The present study describes an NtrC-type Response Regulator (RR) A1S_1978 involved in modulating the metabolism and cell morphology of A. baumannii via a two-component system...
May 9, 2024: Gene
https://read.qxmd.com/read/38734188/linc01605-promotes-malignant-phenotypes-of-cervical-cancer-via-mir-149-3p-wnt7b-axis
#15
JOURNAL ARTICLE
Xiaoyu Kong, Yuanpeng Xiong, Liping Li
BACKGROUND: Long non-coding RNAs (LncRNA) play a pivotal role in the progression of various malignancies. Despite recent identification as an oncogene associated with tumorigenesis. The precise role of LINC01605 in cervical cancer (CC) remains unclear. Therefore, the objective of this study was to investigate the influence of LINC01605 on proliferation and invasion of CC cells, while also exploring its potential underlying mechanisms. METHODS: The expression of LINC01605 in CC cell lines was analyzed using the TCGA database and qRT-PCR...
May 9, 2024: Gene
https://read.qxmd.com/read/38734187/innovative-rpa-pfago-system-for-rapid-mthfr-c677t-polymorphism-identification
#16
JOURNAL ARTICLE
Lianghui Chen, Cheng Wei, Yaqun Liu, Meihua Liao, Jialin Wang, Jiaqi Chen, Peikui Yang, Liejun Li, Chengsong Xie, Min Lin, Zhenxia Zhang, Yuzhong Zheng
This study introduces an efficient RPA-PfAgo detection system for the MTHFR C677T polymorphism, proposing a potential strategy to simplify the genotyping process. By optimizing recombinase polymerase amplification (RPA) with Pyrococcus furiosus Argonaute (PfAgo) nucleases, we achieved DNA amplification at a constant temperature. The assay was fine-tuned through meticulous primer and guide DNA selection, with optimal conditions established at 2.0 µL of MgAc, a reaction temperature of 42 °C, and a 10-minute reaction time for RPA...
May 9, 2024: Gene
https://read.qxmd.com/read/38723785/real-time-pcr-detection-of-pi-s-and-pi-z-alleles-of-serpina1-gene-using-sybr-green
#17
JOURNAL ARTICLE
Ruth Ramos-Díaz, Ainhoa Escuela-Escobar, Ana Díaz-Usera, José María Hernández Pérez, Mario Andrés González-Carracedo, José Antonio Pérez-Pérez
BACKGROUND: Alpha-1 antitrypsin deficiency is an underdiagnosed genetic condition that predisposes to pulmonary complications and is mainly caused by rs28929474 (PI*Z allele) and rs17580 (PI*S allele) mutations in the SERPINA1 gene. OBJECTIVE: Development of a homogeneous genotyping test for detection of PI*S and PI*Z alleles based on the principles of allele-specific PCR and amplicon melting analysis with a fluorescent dye. METHODS: Sixty individuals, which included all possible genotypes that result from combinations of rs28929474 and rs17580 single nucleotide variants, were assayed with tailed allele-specific primers and SYBR Green dye in a real-time PCR machine...
May 7, 2024: Gene
https://read.qxmd.com/read/38723784/development-and-validation-of-the-axiom-marupri-snp-chip-for-genetic-analyses-of-domesticated-old-world-camelids
#18
JOURNAL ARTICLE
Ramesh Kumar Vijh, Upasna Sharma, Reena Arora, Prerna Kapoor, Meenal Raheja, Rekha Sharma, Sonika Ahlawat, Vandana Dureja
Camels play a crucial socio-economic role in sustaining the livelihoods of millions in arid and semi-arid regions. They possess remarkable physiological attributes which enable them to thrive in extreme environments, and provide a source of meat, milk and transportation. With their unique traits, camels embody an irreplaceable source of untapped genomic knowledge. This study introduces Axiom-MaruPri, a medium-density SNP chip meticulously designed and validated for both Camelus bactrianus and Camelus dromedarius...
May 7, 2024: Gene
https://read.qxmd.com/read/38718970/genome-wide-identification-and-functional-analysis-of-the-sicin-gene-family-in-foxtail-millet-setaria-italica-l
#19
JOURNAL ARTICLE
Yongqing Zhao, Tao Wang, Sumei Wan, Yan Tong, Yangyang Wei, Pengtao Li, Nan Hu, Yuling Liu, Hongqi Chen, Xiaoping Pan, Baohong Zhang, Renhai Peng, Shoulin Hu
Cell wall invertase (CIN) is a vital member of plant invertase (INV) and plays a key role in the breakdown of sucrose. This enzyme facilitates the hydrolysis of sucrose into glucose and fructose, which is crucial for various aspects of plant growth and development. However, the function of CIN genes in foxtail millet (Setaria italica) is less studied. In this research, we used the blast-p of NCBI and TBtools for bidirectional comparison, and a total of 13 CIN genes (named SiCINs) were identified from foxtail millet by using Arabidopsis and rice CIN sequences as reference sequences...
May 6, 2024: Gene
https://read.qxmd.com/read/38710294/fengshi-liuhe-decoction-treatment-for-rheumatoid-arthritis-via-the-fzd6-nf-%C3%AE%C2%BAb-signaling-axis
#20
JOURNAL ARTICLE
Fulin Zhou, Peilin Zhou, Xian Jiang, Zhicheng Yang, Weiding Cui, Weixi Liu, Ruiping Liu
To explore whether Fengshi Liuhe Decoction (FLD) alleviates rheumatoid arthritis (RA) via the Fzd6/NF-κB signaling axis. We used real-time quantitative PCR (qPCR) and western blotting (WB) to determine the genes of the frizzled (Fzd) protein 1- Fzd protein 10 that are significantly differentially expressed between normal rat f fibroblast-like synoviocyte (FLS) and collagen II-induced arthritis (CIA) rat FLS. Next, we used enzyme-linked immunosorbent assay (ELISA) to evaluate the levels of inflammatory factors in cell culture supernatant to determine the ability of FLD to ameliorate RA...
May 4, 2024: Gene
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