Metabolomics Analysis Reveals the Effect of Two Alpine Foliar Diseases on the Non-Volatile and Volatile Metabolites of Tea.

Blister blight and small leaf spots are important alpine diseases that mainly attack tender tea leaves, affecting tea quality. However, there is limited information on the effect of these diseases on tea's non-volatile and volatile metabolites. Metabolomic analysis based on UHPLC-Q-TOF/MS, HPLC and GC/MS was used to reveal the characteristic chemical profiles of tea leaves infected with blister blight (BB) and small leaf spots (SS). Flavonoids and monolignols were non-volatile metabolites that were enriched and significantly changed. Six main monolignols involved in phenylpropanoid biosynthesis were significantly induced in infected tea leaves. The accumulation of catechins, (-)-epigallocatechin gallate, (-)-epicatechin gallate, caffeine, amino acids and theanine were significantly decreased in both diseased tea leaves, while soluble sugar, (-)-epigallocatechin and phenol-ammonia were obviously increased. Among them, the amounts of sweet and umami-related soluble sugar, sucrose, amino acids and theanine were much higher in BB, while bitter and astringent taste-related catechins and derivatives were much higher in SS. Volatiles analysis showed that volatiles content in SS and BB was significantly decreased, and styrene was significantly induced in blister blight-infected tea leaves. The results indicate that the type and amount of volatiles were highly and differentially influenced by infection with the two alpine diseases.