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A microscale double labelling of GAG oligosaccharides compatible with enzymatic treatment and mass spectrometry.
Chemical Communications : Chem Comm 2019 April 5
A novel double labelling of glycosaminoglycans (GAG) oligosaccharides by thia-Michael addition and deuterium incorporation at the non-reducing and reducing ends, respectively, was introduced. This was demonstrated to be both compatible with the heparin microgram scale and amenable for mass spectrometry analysis, without impairing enzymatic activities such as heparinase I and sulfatase HSulf-2.
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