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An AIE-based fluorescent probe to detect peroxynitrite levels in human serum and its cellular imaging.
Chemical Communications : Chem Comm 2024 April 31
An AIE-based fluorescent probe was designed to evaluate peroxynitrite levels in complex biological samples. The newly synthesized hydrazone-conjugated probe fluoresces strongly in the presence of peroxynitrite. Clinically, the peroxynitrite levels can be measured in human serum and cellular mitochondria with an LOD of 6.5 nM by fluorescence imaging in vitro .
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