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VPg-based bidirectional synthetic mRNA circuits enable orthogonal protein regulation for high-resolution cell separation.
Chemical Communications : Chem Comm 2024 April 31
Synthetic mRNA circuits commonly sense input to produce binary output signals for cell separation. Based on virus-origin cap-independent translation initiation machinery and RBP-aptamer interaction, we designed smart synthetic mRNA-based circuits that sense single input molecules to bidirectionally tune output signals in an orthogonal manner, enabling high-resolution separation of cell populations.
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