Aruna Ambagala, Kalhari Goonewardene, Ian El Kanoa, Thi Tam Than, Van Tam Nguyen, Thi Ngoc Ha Lai, Thi Lan Nguyen, Cassidy N G Erdelyan, Erin Robert, Nikesh Tailor, Chukwunonso Onyilagha, Lindsey Lamboo, Katherine Handel, Michelle Nebroski, Oksana Vernygora, Oliver Lung, Van Phan Le
In this paper, we report the characterization of a genetically modified live-attenuated African swine fever virus (ASFV) field strain isolated from Vietnam. The isolate, ASFV-GUS-Vietnam, belongs to p72 genotype II, has six multi-gene family (MGF) genes deleted, and an Escherichia coli GusA gene (GUS) inserted. When six 6-8-week-old pigs were inoculated with ASFV-GUS-Vietnam oro-nasally (2 × 105 TCID50 /pig), they developed viremia, mild fever, lethargy, and inappetence, and shed the virus in their oral and nasal secretions and feces...
April 7, 2024: Viruses