Toni Mingers, Stefan Barthels, Violetta Mass, José Manuel Borrero-de Acuña, Rebekka Biedendieck, Ana Cooke, Tamara A Dailey, Svetlana Gerdes, Wulf Blankenfeldt, Harry A Dailey, Martin J Warren, Martina Jahn, Dieter Jahn
Nature utilizes three distinct pathways to synthesize the essential enzyme cofactor heme. The coproporphyrin III-dependent pathway, predominantly present in Bacillaceae , employs an oxygen-dependent coproporphyrinogen III oxidase (CgoX) that converts coproporphyrinogen III into coproporphyrin III. In this study, we report the bioinformatic-based identification of a gene called ytpQ , encoding a putative oxygen-independent counterpart, which we propose to term CgoN, from Priestia ( Bacillus ) megaterium . The recombinantly produced, purified, and monomeric YtpQ (CgoN) protein is shown to catalyze the oxygen-independent conversion of coproporphyrinogen III into coproporphyrin III...
2024: Frontiers in Microbiology