Yuan Wang, Xiaoyu Zhou, Zihan Yang, Tao Xu, Huayang Fu, Chi-Chun Fong, Jiayu Sun, Y Rebecca Chin, Liang Zhang, Xinyuan Guan, Mengsu Yang
Digital DNA amplification is a powerful method for detecting and quantifying rare nucleic acids. In this study, we developed a multi-functional droplet-based platform that integrates the traditional digital DNA amplification workflow into a one-step device. This platform enables efficient droplet generation, transition, and signal detection within a 5-min timeframe, distributing the sample into a uniform array of 4 × 104 droplets (variation <2%) within a chamber. Subsequent in-situ DNA amplification, fluorescence detection, and signal analysis were carried out...
November 14, 2023: Biosensors & Bioelectronics