journal
https://read.qxmd.com/read/38412681/the-value-and-complexity-of-studying-cellular-immunity-against-bk-polyomavirus-in-kidney-transplant-recipients
#21
REVIEW
Aurélien Aubry, Baptiste Demey, Sandrine Castelain, François Helle, Etienne Brochot
BK Polyomavirus is of particular concern for kidney transplant recipients, due to their immunosuppression. This problem is exacerbated by the high effectiveness of antirejection therapies, which also compromise the organism's ability to fight viral infections. The long-term risk is loss of graft function through BKPyV-associated nephropathy (BKPyVAN). The assessment of host immunity and its link to the control of viral infections is a major challenge. In terms of humoral immunity, researchers have highlighted the prognostic value of the pre-transplantation anti-BKPyV immunoglobulin G titer...
April 2024: Journal of Clinical Virology
https://read.qxmd.com/read/38364704/differentiation-of-highly-pathogenic-strains-of-human-jc-polyomavirus-in-neurological-patients-by-next-generation-sequencing
#22
JOURNAL ARTICLE
Eeva Auvinen, Anni Honkimaa, Pia Laine, Sara Passerini, Ugo Moens, Valeria Pietropaolo, Mika Saarela, Leena Maunula, Laura Mannonen, Olli Tynninen, Hannu Haapasalo, Tuomas Rauramaa, Petri Auvinen, Hanna Liimatainen
BACKGROUND: JC polyomavirus (JCPyV) persists asymptomatic in more than half of the human population. Immunocompromising conditions may cause reactivation and acquisition of neurotropic rearrangements in the viral genome, especially in the non-coding control region (NCCR). Such rearranged JCPyV strains are strongly associated with the development of progressive multifocal leukoencephalopathy (PML). METHODS: Using next-generation sequencing (NGS) and bioinformatics tools, the NCCR was characterized in cerebrospinal fluid (CSF; N = 21) and brain tissue (N = 16) samples from PML patients (N = 25), urine specimens from systemic lupus erythematosus patients (N = 2), brain tissue samples from control individuals (N = 2) and waste-water samples (N = 5)...
April 2024: Journal of Clinical Virology
https://read.qxmd.com/read/38237303/enrichment-of-sars-cov-2-sequence-from-nasopharyngeal-swabs-whilst-identifying-the-nasal-microbiome
#23
JOURNAL ARTICLE
Abdulrahman Alrezaihi, Rebekah Penrice-Randal, Xiaofeng Dong, Tessa Prince, Nadine Randle, Malcolm G Semple, Peter J M Openshaw, Tracy MacGill, Todd Myers, Robert Orr, Samo Zakotnik, Alen Suljič, Tatjana Avšič-Županc, Miroslav Petrovec, Miša Korva, Waleed AlJabr, Julian A Hiscox
Simultaneously characterising the genomic information of coronaviruses and the underlying nasal microbiome from a single clinical sample would help characterise infection and disease. Metatranscriptomic approaches can be used to sequence SARS-CoV-2 (and other coronaviruses) and identify mRNAs associated with active transcription in the nasal microbiome. However, given the large sequence background, unenriched metatranscriptomic approaches often do not sequence SARS-CoV-2 to sufficient read and coverage depth to obtain a consensus genome, especially with moderate and low viral loads from clinical samples...
April 2024: Journal of Clinical Virology
https://read.qxmd.com/read/38219682/cytomegalovirus-detected-by-qpcr-in-iris-and-ciliary-body-of-immunocompetent-corneal-donors
#24
JOURNAL ARTICLE
Maxime Rocher, Mathilde Duchesne, Déborah Andouard, Laurence Beral, Marc Labriffe, Delphine Chainier, Mélissa Gomes-Mayeras, Sébastien Hantz, Sophie Alain, Pierre-Yves Robert
BACKGROUND: Cytomegalovirus (CMV) can cause a wide panel of ocular infections. The involvement of CMV as a cause of anterior uveitis in the immunocompetent patient is recent and remains poorly understood. OBJECTIVE: To investigate the presence of CMV in anterior uveal tissues of immunocompetent corneal donors. STUDY DESIGN: We collected aqueous humor, iris, and ciliary body from both eyes of 25 donors died at the Limoges University Hospital between January 2020 and July 2021...
April 2024: Journal of Clinical Virology
https://read.qxmd.com/read/38518504/2023-global-inventory-of-commercial-molecular-tests-for-human-papillomaviruses-hpv
#25
REVIEW
Mario Poljak, Anja Oštrbenk Valenčak, Kate Cuschieri, Klara B Bohinc, Marc Arbyn
To suit the needs of the human papillomaviruses (HPV) community comprehensively, a range of commercial HPV tests with different performance characteristics are required. Four periodic inventories of commercial HPV molecular tests present in the global market were published previously in 2010, 2012, 2015 and 2020. For the fifth inventory, data were retrieved from internal files and a detailed search using the main bibliographic databases as well as general internet search without period or language restrictions was performed in December 2023...
March 14, 2024: Journal of Clinical Virology
https://read.qxmd.com/read/38493709/enterovirus-and-parechovirus-meningoencephalitis-in-infants-a-ten-year-prospective-observational-study-in-a-neonatal-intensive-care-unit
#26
JOURNAL ARTICLE
Carlo Pietrasanta, Andrea Ronchi, Laura Bassi, Agnese De Carli, Luca Caschera, Francesco Maria Lo Russo, Beatrice Letizia Crippa, Silvia Pisoni, Riccardo Crimi, Giacomo Artieri, Laura Pellegrinelli, Robertino Dilena, Giorgio Conte, Fabio Mosca, Monica Fumagalli, Lorenza Pugni
BACKGROUND: Non-polio enteroviruses (EV) and human parechoviruses (HPeV) are known etiological agents of meningoencephalitis in neonates. However, reports of neuroradiological findings and neurodevelopmental outcomes in this population are scarce. OBJECTIVES: to describe clinical characteristics, neuroradiological findings and, in a subset of patients, neurodevelopmental outcomes in a cohort of infants with EV or HPeV meningoencephalitis within 60 days of life. STUDY DESIGN: clinical/laboratory data, neuroradiological findings (cranial ultrasound, cUS, brain magnetic resonance imaging, MRI), and neurodevelopmental outcomes assessed by Ages and Stages Questionnaires - third edition were prospectively collected...
March 5, 2024: Journal of Clinical Virology
https://read.qxmd.com/read/38471301/evaluation-of-the-elitechgroup-solution-for-plasma-hiv-1-rna-quantification
#27
JOURNAL ARTICLE
Adeline Baron, Alice Moisan, Guillemette Unal, Véronique Lemée, Manuel Etienne, Jean-Christophe Plantier
In the last few years, many manufacturers have developed new kits for plasma HIV-1 RNA quantification. Recently, a solution consisting of the ELITe InGenius® instrument and the HIV1 ELITe MGB®kit has been commercialized worldwide. Our aim was to compare its clinical performance with the Aptima® HIV-1 Quant Dx kit by Hologic, on a panel of HIV-1 group M circulating variants, representative of viral load levels found during the pre- and post-treatment follow-up of patients. The linearity was evaluated on the AcroMetrix® HIV-1 Panel...
March 3, 2024: Journal of Clinical Virology
https://read.qxmd.com/read/38503118/immunogenicity-and-safety-of-covid-19-booster-vaccination-a-population-based-clinical-trial-to-identify-the-best-vaccination-strategy
#28
JOURNAL ARTICLE
Daniela Sieghart, Claudia A Hana, Caroline Dürrschmid, Leonhard X Heinz, Helmuth Haslacher, Markus Zlesak, Giulia Piccini, Alessandro Manenti, Emanuele Montomoli, Anselm Jorda, Clemens Fedrizzi, Timothy Hasenoehrl, Andrej Zdravkovic, Karolina Anderle, Ursula Wiedermann, Susanne Drapalik, Helmut Steinbrecher, Felix Bergmann, Christa Firbas, Galateja Jordakieva, Barbara Wagner, Margherita Leonardi, Giulio Pierleoni, Matilde Ballini, Linda Benincasa, Serena Marchi, Claudia Trombetta, Thomas Perkmann, Richard Crevenna, Markus Zeitlinger, Michael Bonelli, Daniel Aletaha, Helga Radner
BACKGROUND: Various SARS-CoV-2 variants of concerns (VOCs) characterized by higher transmissibility and immune evasion have emerged. Despite reduced vaccine efficacy against VOCs, currently available vaccines provide protection. Population-based evidence on the humoral immune response after booster vaccination is crucial to guide future vaccination strategies and in preparation for imminent COVID-19 waves. METHODS: This multicenter, population-based cohort study included 4697 individuals ≥18 years of age who received a booster vaccination...
February 28, 2024: Journal of Clinical Virology
https://read.qxmd.com/read/38432097/human-monkeypox-virus-epidemiologic-review-and-research-progress-in-diagnosis-and-treatment
#29
REVIEW
Yanhong Sun, Wenjian Nie, Dandan Tian, Qing Ye
Monkeypox virus (MPXV) is responsible for causing a zoonotic disease called monkeypox (mpox), which sporadically infects humans in West and Central Africa. It first infected humans in 1970 and, along with the variola virus, belongs to the genus Orthopoxvirus in the poxvirus family. Since the World Health Organization declared the MPXV outbreak a "Public Health Emergency of International Concern" on July 23, 2022, the number of infected patients has increased dramatically. To control this epidemic and address this previously neglected disease, MPXV needs to be better understood and reevaluated...
February 28, 2024: Journal of Clinical Virology
https://read.qxmd.com/read/38412680/corrigendum-to-first-international-proficiency-study-on-human-papillomavirus-testing-in-cervical-cancer-screening-j-clin-virol-2023-oct-167-105581
#30
Emel Yilmaz, Carina Eklund, Camilla Lagheden, Karin Dahlin Robertsson, Marina Lilja, Miriam Elfström, Laila Sara Arroyo Mühr, Joakim Dillner
No abstract text is available yet for this article.
February 26, 2024: Journal of Clinical Virology
https://read.qxmd.com/read/38447459/one-health-in-action-investigation-of-the-first-detected-local-cluster-of-fatal-borna-disease-virus-1-bodv-1-encephalitis-germany-2022
#31
JOURNAL ARTICLE
Merle M Böhmer, Viola C Haring, Barbara Schmidt, Franziska S Saller, Liza Coyer, Lidia Chitimia-Dobler, Gerhard Dobler, Dennis Tappe, Andrea Bonakdar, Arnt Ebinger, Gertrud Knoll, Lisa Eidenschink, Anette Rohrhofer, Hans Helmut Niller, Katharina Katz, Philip Starcky, Martin Beer, Rainer G Ulrich, Dennis Rubbenstroth, Markus Bauswein
BACKGROUND: Zoonotic Borna disease virus 1 (BoDV-1) causes fatal encephalitis in humans and animals. Subsequent to the detection of two paediatric cases in a Bavarian municipality in Germany within three years, we conducted an interdisciplinary One Health investigation. We aimed to explore seroprevalence in a local human population with a risk for BoDV-1 exposure as well as viral presence in environmental samples from local sites and BoDV-1 prevalence within the local small mammal population and its natural reservoir, the bicoloured white-toothed shrew (Crocidura leucodon)...
February 23, 2024: Journal of Clinical Virology
https://read.qxmd.com/read/38430669/performance-of-the-xpert%C3%A2-mpox-pcr-assay-with-oropharyngeal-anorectal-and-cutaneous-lesion-swab-specimens
#32
JOURNAL ARTICLE
Gregory L Damhorst, Kaleb McLendon, Evelyn Morales, Zianya M Solis, Eric Fitts, Heather B Bowers, Courtney Sabino, Julie Sullivan, Morgan Greenleaf, John D Roback, Jonathan A Colasanti, Anandi N Sheth, Boghuma K Titanji, Greg S Martin, Leda Bassit, Wilbur A Lam, Anuradha Rao
Anorectal and oropharyngeal exposures are implicated in sexual transmission of mpox, but authorized assays in the United States are only validated with cutaneous lesion swabs. Diagnostic assays for anorectal and oropharyngeal swabs are needed to address potential future outbreaks. The Cepheid Xpert® Mpox is the first point-of-care assay to receive FDA emergency use authorization in the United States and would be a valuable tool for evaluating these sample types. Our exploratory study demonstrates 100 % positive agreement with our in-house PCR assay for natural positive anorectal and oropharyngeal specimens and 92 % sensitivity with low-positive spiked specimens...
February 23, 2024: Journal of Clinical Virology
https://read.qxmd.com/read/38401369/human-papillomavirus-negative-high-grade-cervical-lesions-and-cancers-suggested-guidance-for-hpv-testing-quality-assurance
#33
JOURNAL ARTICLE
Jean Luc Prétet, Laila Sara Arroyo Mühr, Kate Cuschieri, María Dolores Fellner, Rita Mariel Correa, María Alejandra Picconi, Suzanne M Garland, Gerald L Murray, Monica Molano, Michael Peeters, Steven Van Gucht, Charlotte Lambrecht, Davy Vanden Broeck, Elizaveta Padalko, Marc Arbyn, Quentin Lepiller, Alice Brunier, Steffi Silling, Kristiane Søreng, Irene Kraus Christiansen, Mario Poljak, Camilla Lagheden, Emel Yilmaz, Carina Eklund, Hem R Thapa, Troy D Querec, Elizabeth R Unger, Joakim Dillner
BACKGROUND: Some high-grade cervical lesions and cervical cancers (HSIL+) test negative for human papillomavirus (HPV). The HPV-negative fraction varies between 0.03 % and 15 % between different laboratories. Monitoring and extended re-analysis of HPV-negative HSIL+ could thus be helpful to monitor performance of HPV testing services. We aimed to a) provide a real-life example of a quality assurance (QA) program based on re-analysis of HPV-negative HSIL+ and b) develop international guidance for QA of HPV testing services based on standardized identification of apparently HPV-negative HSIL+ and extended re-analysis, either by the primary laboratory or by a national HPV reference laboratory (NRL)...
February 20, 2024: Journal of Clinical Virology
https://read.qxmd.com/read/38387136/rapid-antigen-testing-for-sars-cov-2-by-lateral-flow-assay-a-field-evaluation-of-self-and-professional-testing-at-uk-community-testing-sites
#34
JOURNAL ARTICLE
Matthias E Futschik, Samuel Johnson, Elena Turek, David Chapman, Simon Carr, Zareen Thorlu-Bangura, Paul E Klapper, Malur Sudhanva, Andrew Dodgson, Joanna R Cole-Hamilton, Nick Germanacos, Raghavendran Kulasegaran-Shylini, Edward Blandford, Sarah Tunkel, Timothy Peto, Susan Hopkins, Tom Fowler
BACKGROUND: The advent of lateral flow devices (LFDs) for SARS-CoV-2 detection enabled widespread use of rapid self-tests during the pandemic. While self-testing using LFDs is now common, whether self-testing provides comparable performance to professional testing was a key question that remained important for pandemic planning. METHODS: Three prospective multi-centre studies were conducted to compare the performance of self- and professional testing using LFDs...
February 15, 2024: Journal of Clinical Virology
https://read.qxmd.com/read/38408420/evaluation-of-genexpert-and-advanced-biological-laboratories-ultragene-hcv-diagnostic-detection-and-performance-against-roche-real-time-pcr-in-myanmar
#35
JOURNAL ARTICLE
Pedro Pisa, Constance Wose Kinge, Charles Chasela, Eula Mothibi, Yin Min Thaung, Hnin T Thwin, Nay M Aung, Kara W Chew, Malini M Gandhi, Cavenaugh Clint, Thomas Minior, Aye A Lwin, Morgan J Freiman, Khin P Kyi, Yi Y Sein, Fadzai Marange, Charles van der Horst, Sofiane Mohamed, Matthieu Barralon, Ian Sanne
BACKGROUND: Developing countries experience limited access to HCV laboratory tests for different reasons. Providing near to real-time HCV testing and results especially to at-risk populations including those in rural settings for timely initiation to treatment is key. Within a rural Myanmar setting, we compared HCV diagnostic detection and quantification of the GeneXpert, and Advanced Biological Laboratories UltraGene-HCV assays against the gold standard and reference method Roche real-time HCV in Myanmar...
February 12, 2024: Journal of Clinical Virology
https://read.qxmd.com/read/38367294/quality-control-data-management-with-unity-real-time-in-molecular-virology
#36
JOURNAL ARTICLE
Mikayla Quinton, Duane W Newton, Becky Neil, Sondra Mitchell, Heba H Mostafa
INTRODUCTION: Quality control (QC) is one component of an overarching quality management system (QMS) that aims at assuring laboratory quality and patient safety. QC data must be acceptable prior to reporting patients' results. Traditionally, QC statistics, records, and corrective actions were tracked at the Johns Hopkins Molecular Virology Laboratory using Microsoft Excel. Unity Real-Time (UnityRT), a QMS software (Bio-Rad Laboratories), which captures and analyzes QC data by instrument and control lot per assay, was implemented and its impact on the workflow was evaluated...
February 12, 2024: Journal of Clinical Virology
https://read.qxmd.com/read/38346360/quantiferon-cmv-assay-by-chemiluminescence-immunoassay-is-it-more-suitable-for-real-live-monitoring-of-transplant-patients
#37
JOURNAL ARTICLE
Raquel Fernández-Moreno, Aurora Páez-Vega, Diego Rodríguez-Cano, Ana Salinas, Fernando Rodríguez-Cantalejo, Aurora Jurado, Julián Torre-Cisneros, Sara Cantisán
BACKGROUND: The QuantiFERONCMV (QF-CMV) assay is an interferon-gamma release assay (IGRA) used to monitor CMV-specific cell-mediated immunity (CMV-CMI) by ELISA in transplant patients. However, a chemiluminescent immunoassay (CLIA) has been developed to quantify IFNG in the QuantiFERON-Tuberculosis (TB) to detect latent TB infection. OBJECTIVES: The aim of this work is to compare the results of QF-CMV by ELISA with those obtained by CLIA in an automated Liaison XL analyzer using the QuantiFERON-TB Gold Plus reagents...
February 8, 2024: Journal of Clinical Virology
https://read.qxmd.com/read/38350177/impact-of-anti-hdv-reflex-testing-at-hbs-antigen-positive-discovery-in-a-single-center-france-support-for-primary-hdv-screening-in-france
#38
JOURNAL ARTICLE
Assilina Parfut, Simona Tripon, Pierre Gantner, Fréderic Chaffraix, Elodie Laugel, Marie-Josée Wendling, Furkan Erol, Carine Wiedemer, Michel Doffoel, Antonio Saviano, Maude Royant, François Habersetzer, Samira Fafi-Kremer, Aurélie Velay
BACKGROUND: Hepatitis Delta virus (HDV) infection is a major cause of liver-related morbidity and mortality in patients infected with HBV, with a global HDV prevalence uncertain. In France, 2 to 5 % of HBs antigen (HBsAg) carriers present anti-HDV antibodies (anti-HDV). The EASL recommends testing for anti-HDV in all HBsAg-positive patients. Since January 2022, we have systematically carried out anti-HDV serology when a positive HBsAg is discovered (new HBsAg carriers). OBJECTIVES: We evaluated the benefit of anti-HDV reflex testing after one year of practice by comparing anti-HDV and HBsAg serology data over the last six years, among the new HBsAg carriers and all the HBsAg carriers...
February 3, 2024: Journal of Clinical Virology
https://read.qxmd.com/read/38335717/validation-of-the-clinical-performance-and-reproducibility-of-the-neumodx-hpv-assay-self-sample-workflow
#39
JOURNAL ARTICLE
D A M Heideman, J Berkhof, L Verhoef, C Ouwerkerk, P W Smit, A Oštrbenk Valenčak, J Mlakar, M Poljak, R D M Steenbergen, M C G Bleeker
BACKGROUND: Human papillomavirus (HPV) testing on self-samples is a valid tool for cervical cancer screening. HPV self-sample workflows need to be clinically validated to ensure safe use in screening. OBJECTIVE: This study evaluated the fully automated NeuMoDx HPV Assay self-sample workflow that is compiled of the NeuMoDx HPV assay and the NeuMoDx 96/288 Molecular Systems, for clinical performance and reproducibility on Evalyn Brush-collected self-samples. METHODS: The clinical performance of the NeuMoDx HPV Assay self-sample workflow for cervical intraepithelial neoplasia grade 2 or worse (CIN2+) and CIN3+ was evaluated on 987 self-samples obtained from women attending national organized HPV-based cervical cancer screening by a noninferiority analysis relative to reference workflows using either HPV-Risk Assay or high-risk HPV GP5+/6+-PCR...
February 1, 2024: Journal of Clinical Virology
https://read.qxmd.com/read/38309173/don-t-rash-it-the-clinical-significance-of-positive-varicella-zoster-virus-pcr-in-cerebrospinal-fluid-of-patients-with-neurological-symptoms
#40
JOURNAL ARTICLE
Or Kriger, Sarah Dovrat, Ilana S Fratty, Eyal Leshem, Michal Tepperberg Oikawa, Danit Sofer, Sharon Amit
BACKGROUND: Varicella zoster virus (VZV) is among the leading pathogens causing meningitis and encephalitis. While VZV-PCR-positive CSF is considered a gold-standard for diagnosis, it is not-uncommon to detect VZV-DNA in CSF of patients with other acute or chronic illness. Our goal was to determine the clinical relevance of VZV-PCR-positive CSF when investigating patients with neurological symptoms. METHODS: In this retrospective cohort from the largest hospital in Israel, we collected demographic, clinical and laboratory data of patients with VZV-PCR-positive CSF, analyzing the significance of various parameters...
January 22, 2024: Journal of Clinical Virology
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