Julia Melchert, Helena Radbruch, Leif G Hanitsch, Sally A Baylis, Jörn Beheim-Schwarzbach, Tobias Bleicker, Jörg Hofmann, Terry C Jones, Christian Drosten, Victor M Corman
BACKGROUND: Hepatitis E virus (HEV) is a leading cause of acute hepatitis and can cause chronic infections in immunocompromised patients. Although HEV infections can be treated with ribavirin, antiviral efficacy is hampered by resistance mutations, normally detected by virus sequencing. OBJECTIVES: High-throughput sequencing (HTS) allows for cost-effective complete viral genome sequencing. This enables the discovery and delineation of new subtypes, and revised the recognition of quasispecies and putative resistance mutations...
September 12, 2023: Journal of Clinical Virology
Paul Kinsella, Michael Moso, Genevieve Martin, Theo Karapangiotidis, Di Karamalakis, Suellen Nicholson, Mitch Batty, Kathy Jackson, Madeleine Marsland, Tilda Thompson, Lakshmi Manoharan, Helen O'brien, Deborah Friedman, Katherine Bond, Deborah A Williamson, Chuan Kok Lim
The unexpected recent emergence of Japanese encephalitis virus (JEV) genotype IV in multiple southern states of Australia necessitated an evaluation of JEV serological tests suitable for diagnosing acute infection and for seroprevalence studies. This study examined the analytical and clinical performance of two high-throughput JEV assays, Euroimmun immunofluorescence assay (IFA) and Euroimmun enzyme-linked immunosorbent assay (ELISA), across four cohorts; (1) surveillance of piggery workers in outbreak areas, (2) surveillance of residents in outbreak areas, (3) acute JEV infection and (4) post-JEV vaccination...
September 9, 2023: Journal of Clinical Virology
Emel Yilmaz, Carina Eklund, Camilla Lagheden, Karin Dahlin Robertsson, Marina Lilja, Miriam Elfström, Laila Sara Arroyo Mühr, Joakim Dillner
BACKGROUND: Although cervical screening using Human Papillomavirus (HPV) testing is globally recommended public health policy, there has been no international proficiency studies specifically targeting HPV testing for cervical screening. OBJECTIVE: To obtain the first global overview of the current proficiency of HPV testing services for cervical cancer screening. STUDY DESIGN: A coded proficiency panel of 12 samples containing HPV types 16, 18, 31, 33, 45, 52, 58 or 35/39/51/56/59/68 in human DNA in varying amounts as well as control...
September 4, 2023: Journal of Clinical Virology
Lene Ugilt Pagter Ludvigsen, Annemette Sloth Andersen, Stephen Hamilton-Dutoit, Søren Jensen-Fangel, Pernille Bøttger, Kurt Jensen Handberg, Per Ivarsen, Francesco d'Amore, Bo Martin Bibby, Birgitte Klug Albertsen, Bente Jespersen, Marianne Kragh Thomsen
BACKGROUND: Quantitative polymerase chain reaction (qPCR) for Epstein-Barr virus (EBV)-DNA is an important diagnostic tool for EBV-associated disease, but interpretation of its clinical significance is challenging. OBJECTIVES: We assessed the diagnostic and clinical performance of WHO-standardised qPCR for EBV-DNA (WHO EBV-qPCR) in plasma and whole blood (WB) for proven EBV disease in a prospectively accrued patient cohort. STUDY DESIGN: Central Denmark Region patients, tested with WHO EBV-qPCR from November 2017 to March 2019, were screened for EBV disease...
August 30, 2023: Journal of Clinical Virology
Carmen de Mendoza, Ariadna Rando, Elisenda Miró, María José Pena, Iciar Rodríguez-Avial, Diego Ortega, Alejandro González-Praetorius, Gabriel Reina, Ilduara Pintos, María José Pozuelo, Vicente Soriano
BACKGROUND: HTLV-1 infection is a neglected disease, despite producing neurological and lymphoproliferative severe illnesses and affect over 10 million people worldwide. Roughly 5% of HTLV-1 carriers develop Adult T-cell leukemia/lymphoma (ATLL), one of the most aggressive hematological malignancies. METHODS: A national HTLV-1 register exists since 1989 in Spain, a non-endemic country with a large migrant flow from Latin America and Equatorial Africa, where HTLV-1 is endemic...
August 28, 2023: Journal of Clinical Virology
Dana Danino, Guy Hazan, Rofaida Mahajna, Firas Khalde, Lama Farraj, Yonat Shemer Avni, David Greenberg, Eli Hershkovitz, Yaniv Faingelernt, Noga Givon-Lavi
BACKGROUND: Multiplex-PCR is a valuable tool for diagnosing viral acute gastroenteritis (AGE), enabling the detection of multiple pathogens. However, distinguishing between active disease and shedding poses challenges. This study aimed to evaluate viral AGE epidemiology and compare clinical characteristics among the five most common viruses. METHODS: Rotavirus vaccine was introduced in 2010, with 70% coverage achieved in southern Israel in two years. All rectal swabs for multiplex-PCR targeting rotavirus, norovirus, adenovirus, astrovirus and sapovirus from hospitalized diarrheic children <5 years were included, from December 2017 through March 2022...
August 25, 2023: Journal of Clinical Virology
Jacques Fourgeaud, Slimane Allali, Julie Toubiana, Yael Pinhas, Pierre Frange, Marianne Leruez-Ville, Jérémie F Cohen
No abstract text is available yet for this article.
August 22, 2023: Journal of Clinical Virology
Jamie Fagg, Rupert Beale, Matthias E Futschik, Elena Turek, David Chapman, Susan Halstead, Marc Jones, Joanna Cole-Hamilton, Rory Gunson, Malur Sudhanva, Paul E Klapper, Harper Vansteenhouse, Sarah Tunkel, Anna Dominiczak, Timothy Ea Peto, Tom Fowler
BACKGROUND: The challenges of rapid upscaling of testing capacity were a major lesson from the COVID-19 pandemic response. The need for process adjustments in high-throughput testing laboratories made sample pooling a challenging option to implement. OBJECTIVE: This study aimed to evaluate whether pooling samples at source (swab pooling) was as effective as qRT-PCR testing of individuals in identifying cases of SARS-CoV-2 in real-world community testing conditions using the same high-throughput pipeline...
August 19, 2023: Journal of Clinical Virology
Giuseppe Sberna, Roberta Nardacci, Giulia Berno, Gabriella Rozera, Emanuela Giombini, Lavinia Fabeni, Eliana Specchiarello, Fabrizio Maggi, Alessandra Amendola
BACKGROUND: In a restricted subset of HIV patients with suppressed viral load (i.e., pol-undetected HIV-RNA), the Aptima HIV-1 Quant Dx Assay (Aptima), a dual-target (pol and LTR) and dual-probe test for viral load (VL) monitoring, can detect HIV-RNA exclusively through amplification of the LTR region. OBJECTIVES: To analyze the virological characteristics of the HIV-RNA elements detected only through LTR amplification (LTR-e). STUDY DESIGN: LTR-e isolated from plasma and peripheral blood mononuclear cells (PBMC) were evaluated for their ability to trigger productive infections...
August 15, 2023: Journal of Clinical Virology
Jocelyn Hume, Kym Lowry, David M Whiley, Adam D Irwin, Cheryl Bletchly, Emma L Sweeney
BACKGROUND: Cytomegalovirus (CMV) is a viral infection which establishes lifelong latency, often reactivating and causing disease in immunosuppressed individuals, including haematopoietic stem cell transplant (HSCT) recipients. Treatment can be problematic due to antiviral resistance which substantially increases the risk of patient mortality. Diagnostic testing capabilities for CMV antiviral resistance in Australia and elsewhere have traditionally relied on gene-specific Sanger sequencing approaches, however, are now being superseded by next generation sequencing protocols...
August 4, 2023: Journal of Clinical Virology
Eliza Gil, Sunando Roy, Tim Best, James Hatcher, Judith Breuer
BACKGROUND: Rhinovirus (HRV) is a significant seasonal pathogen in children. The emergence of SARS-CoV2, and the social restrictions introduced in, disrupted viral epidemiology. Here we describe the experience of Great Ormond Street Hospital (GOSH), where HRV almost entirely disappeared from the paediatric intensive care units (PICU) during the first national lockdown and then rapidly re-emerged with a fast-increasing incidence, leading to concerns about possible nosocomial transmission in a vulnerable population...
July 30, 2023: Journal of Clinical Virology
Heather L Glasgow, Haiying Zhu, Hong Xie, Elizabeth J Kenkel, Carrie Lee, Meei-Li Huang, Alexander L Greninger
BACKGROUND: Antiviral resistance in human herpes simplex viruses (HSV) remains a significant clinical challenge in immunocompromised populations. Although molecular tests have largely replaced viral culture for HSV diagnosis and molecular antiviral resistance testing is available for many viruses, HSV resistance testing continues to rely on phenotypic, viral culture-based methods, requiring weeks for results. Consequently, treatment of suspected HSV resistance remains largely empiric...
July 29, 2023: Journal of Clinical Virology
José Manuel Ramos-Rincon, Héctor Pinargote-Celorio, Carmen de Mendoza, Clara Ramos-Belinchón, Víctor Moreno-Torres, Ana Treviño, Pablo Barreiro, Octavio Corral, Vicente Soriano
BACKGROUND: Before the advent of COVID-19 vaccines, hospitalizations due to SARS-CoV-2 infection during 2020 collapsed most medical centers worldwide. Disruptions in health care for clinical conditions other than COVID-19 were not uniform. Herein, we report the impact of COVID-19 on hospitalizations due to viral hepatitis in Spain. METHODS: Retrospective study of all hospitalizations in Spain during 10 months before (pre-pandemic period) and after (pandemic period) March 1st 2020...
July 27, 2023: Journal of Clinical Virology
D Armenia, R Gagliardini, C Alteri, V Svicher, V Cento, V Borghi, A Vergori, S Cicalini, F Forbici, L Fabeni, A Bertoli, M Brugneti, W Gennari, V Malagnino, M Andreoni, C Mussini, A Antinori, C F Perno, M M Santoro, F Ceccherini-Silberstein
BACKGROUND: We aimed at evaluating the temporal trend of drug-resistance and APOBEC editing from HIV-DNA genotypic resistance tests (GRT) in virologically suppressed individuals. MATERIAL AND METHODS: Major resistance mutations (MRM), genotypic susceptibility score (GSS) for the current regimen and APOBEC-related mutations (APO-M) were evaluated. Potential changes in trends of MRM and APO-M over-time were assessed and predictors of MRM detection or sub-optimal GSS (GSS<2) at HIV-DNA-GRT were estimated through logistic regression analyses...
July 23, 2023: Journal of Clinical Virology
Yi Sun, Jian Cai, Haiyan Mao, Liming Gong, Yin Chen, Hao Yan, Wen Shi, Xiuyu Lou, Lingxuan Su, Xingxing Wang, Biaofeng Zhou, Zhichao Pei, Yanli Cao, Qiong Ge, Yanjun Zhang
BACKGROUND: Coxsackievirus A10 (CA10) is one of the etiological agents associated with hand, foot and mouth disease (HFMD). OBJECTIVES: We aimed to perform a retrospective analysis of the molecular epidemiological characteristics and genetic features of HFMD associated with CA10 infections in Zhejiang Province from 2017 to 2022. STUDY DESIGN: Epidemiologic features were summarized. Throat swab specimens were collected and tested. The VP1 regions were sequenced for genotyping...
July 23, 2023: Journal of Clinical Virology
Sarah Mafi, Sophie Alain, Sébastien Hantz
BACKGROUND: Monitoring CMV-specific cell-mediated immunity by the QuantiFERON®-CMV (QF-CMV) may be useful in predicting the risk of CMV infection in transplant recipients (TR). OBJECTIVES: As the QuantiFERON-Tuberculosis (QFT®-Plus) became available on the fully automated LIAISON®XL chemiluminescence (CLIA) analyzer, we evaluated the performance of the QF-CMV on the LIAISON®XL analyzer using the QuantiFERON®-TB Gold Plus reagent. STUDY DESIGN: Between 2018 and 2022, 81 samples from TR were collected at the Department of Virology of Limoges Hospital, France...
July 20, 2023: Journal of Clinical Virology
Lynne Li, Christopher F Lowe, Elizabeth McLachlan, Marc G Romney, Alissa Wright, Nancy Matic
BACKGROUND: CMV reactivation post-transplantation is common, with need for prompt identification of patients most at-risk for CMV antiviral drug resistance (AVDR). OBJECTIVES: This study describes CMV AVDR frequencies, antiviral prescribing practices, and AVDR risk factors in patients from 2011 to 2019 in British Columbia, Canada. STUDY DESIGN: Retrospective review of demographics, transplant type, viral loads, antiviral exposure duration, and 12-month mortality was conducted for all patients with samples submitted for CMV AVDR testing from 2011 to 2019...
July 18, 2023: Journal of Clinical Virology
Hem R Thapa, Elizabeth R Unger, Troy D Querec
Well characterized reference reagents are useful for assay validation, proficiency/competency assessment, daily run controls, and to improve inter-laboratory comparisons. Synthetic human papillomavirus (HPV) DNA fragments and plasmid clones are available, but synthetic fragments include limited segments of the HPV genome and many HPV plasmids have interrupted coding regions or contain partial genomes. As a result, they are not compatible with all HPV DNA detection and typing assays. To address this need, we are establishing an HPV plasmid repository of HPV clones containing the whole genome of each type with no interruptions in coding regions...
July 17, 2023: Journal of Clinical Virology
Shi Zhao, Zihao Guo, Shengzhi Sun, Chi Tim Hung, Eman Yee Man Leung, Yuchen Wei, Huwen Wang, Kehang Li, Carrie Ho Kwan Yam, Tsz Yu Chow, Jian Gao, Katherine Min Jia, Ka Chun Chong, Eng-Kiong Yeoh
BACKGROUND: In 2022, SARS-CoV-2 Omicron variants circulated globally, generating concerns about increased transmissibility and immune escape. Hong Kong, having an infection-naive population with a moderate 2-dose vaccine coverage (63% by the end of 2021), experienced a COVID-19 epidemic largely seeded by Omicron BA.2 variants that led to the greatest outbreak in the region to date. Little remains known about the protection of commonly-administered vaccines against transmission of Omicron BA...
July 10, 2023: Journal of Clinical Virology
Alexander Kiefer, Sylvia Pemmerl, Michael Kabesch, Andreas Ambrosch
BACKGROUND: RSV is an important cause for respiratory illness in children and the elderly. We analysed RSV seasons since 2016 in both age groups for differences, similarities and timely associations before, during and after the COVID-19 pandemic. METHODS: We studied epidemiological and clinical features of seven consecutive RSV seasons since 2016 retrospectively in children and adults who were systematically monitored for RSV infections by PCR when hospitalized in Regensburg, Germany...
July 6, 2023: Journal of Clinical Virology
Fetch more papers »
Fetching more papers... Fetching...
Remove bar
Read by QxMD icon Read

Save your favorite articles in one place with a free QxMD account.


Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"

We want to hear from doctors like you!

Take a second to answer a survey question.