Rosa Montero-Macías, David Veyer, Thomas Bruneau, Nicolas Robillard, Marie-Aude Le Frère-Belda, Pascal Rigolet, Mélanie Boulhic, Ivana Stankovic, Laurent Bélec, Martina Aida Angeles, Eliane Mery, Cécile Badoual, Pluvio J Coronado, Anne-Sophie Bats, Patrice Mathevet, Valérie Taly, Fabrice Lécuru, Hélène Péré
INTRODUCTION: In early cervical cancer (EEC), 10 to 15% of patients without nodal metastasis (N-) will suffer from recurrences with further similar survival as N+ patients. However, no clinical, imaging or pathological risk-factor is today available to identify them. In the present study, we hypothesized that the N- histologically characterized patients who present a poor prognosis could be patients for whom metastasis are missed by classical procedure. Therefore, we propose to research HPV tumoral DNA (HPVtDNA) in pelvic Sentinel Lymph Nodes (SLN) biopsy using ultrasensitive droplet-based digital PCR (ddPCR) to detect eventual occult metastasis...
March 8, 2023: Journal of Clinical Virology
Paul K Drain, Ronit R Dalmat, Linhui Hao, Meagan J Bemer, Elvira Budiawan, Jennifer F Morton, Renee C Ireton, Tien-Ying Hsiang, Zarna Marfatia, Roshni Prabhu, Claire Woosley, Adanech Gichamo, Elena Rechkina, Daphne Hamilton, Michalina Montaño, Jason L Cantera, Alexey S Ball, Inah Golez, Elise Smith, Alexander L Greninger, M Juliana McElrath, Matthew Thompson, Benjamin D Grant, Allison Meisner, Geoffrey S Gottlieb, Michael J Gale
BACKGROUND: Guidelines for SARS-CoV-2 have relied on limited data on duration of viral infectiousness and correlation with COVID-19 symptoms and diagnostic testing. METHODS: We enrolled ambulatory adults with acute SARS-CoV-2 infection and performed serial measurements of COVID-19 symptoms, nasal swab viral RNA, nucleocapsid (N) and spike (S) antigens, and replication-competent SARS-CoV-2 by viral growth in culture. We determined average time from symptom onset to a first negative test result and estimated risk of infectiousness, as defined by positive viral growth in culture...
March 3, 2023: Journal of Clinical Virology
Chuan Kok Lim, Charlene McKenzie, Joshua Deerain, Eric P F Chow, Janet Towns, Marcus Y Chen, Christopher K Fairley, Thomas Tran, Deborah A Williamson
BACKGROUND: In the 2022 mpox outbreak, several studies have explored longitudinal DNA shedding of mpox virus (MPXV) using PCR. However, there are fewer studies assessing infectivity in cell culture, and, by inference, MPXV transmissibility. Such information could help inform infection control and public health guidelines. AIMS AND METHODS: The aim of this study was to correlate cell culture infectivity of clinical samples with viral loads in clinical samples. Between May to October 2022, clinical samples from different body sites sent to the Victorian Infectious Diseases Reference Laboratory in Melbourne, Australia for MPXV PCR detection were cultured in Vero cells as a surrogate for infectivity...
March 3, 2023: Journal of Clinical Virology
Jean-Louis Bayart, Constant Gillot, Jean-Michel Dogné, Gatien Roussel, Valérie Verbelen, Julien Favresse, Jonathan Douxfils
BACKGROUND: A SARS-CoV-2+Flu A/B+RSV Combo Rapid test may be more relevant than Rapid Antigen Diagnostic (RAD) tests targeting only SARS-CoV-2 since we are facing a concurrent circulation of these viruses during the winter season. OBJECTIVES: To assess the clinical performance of a SARS-CoV-2+Flu A/B+RSV Combo test in comparison to a multiplex RT-qPCR. STUDY DESIGN: Residual nasopharyngeal swabs issued from 178 patients were included. All patients, adults and children, were symptomatic and presented at the emergency department with flu-like symptoms...
February 28, 2023: Journal of Clinical Virology
Ambrosch Andreas, Luber Doris, Klawonn Frank, Kabesch Michael
BACKGROUND: The role and impact of RSV in the adult population is not well understood and comparative data of RSV infection, influenza A/B and SARS-CoV-2 in the elderly hospitalized for respiratory infections is limited. METHODS: In a retrospective, monocentric study we analyzed data of adult patients with respiratory infections tested positive by PCR for RSV, Influenza A/B and SARS-CoV-2 over a four-year period from 2017 to 2020. Symptoms on admission, laboratory results, and risk factors were assessed, and the clinical course and outcomes were studied...
February 14, 2023: Journal of Clinical Virology
Alexander Domnich, Bianca Bruzzone, Carlo-Simone Trombetta, Vanessa De Pace, Valentina Ricucci, Serena Varesano, Giada Garzillo, Matilde Ogliastro, Andrea Orsi, Giancarlo Icardi
BACKGROUND: Influenza and respiratory syncytial (RSV) viruses are expected to co-circulate with SARS-CoV-2 in the upcoming seasons and clinical differential diagnosis between them is difficult. Laboratory-based RT-PCR is a gold standard diagnostic method for influenza, RSV and SARS-CoV-2. The objective of this study was to estimate the diagnostic performance of a novel point-of-care RT-PCR assay STANDARD M10 Flu/RSV/SARS-CoV-2 (SD Biosensor) in a large number of clinical specimens with diversified (co)-infection patterns and viral loads...
February 14, 2023: Journal of Clinical Virology
Amary Fall, Michael Forman, C Paul Morris, Victoria Gniazdowski, Chun Huai Luo, Ann Hanlon, Heather Miller, Yehudit Bergman, Heba H Mostafa
BACKGROUND: Enteroviruses (EVs) are predominant causes of a spectrum of neurological diseases. To better understand the origins of the outbreaks of disease associated with EV, it is essential to develop an efficient surveillance system that identifies the circulating EVs and correlate their genomic evolution with the disease presentations. METHODS: The clinical presentations of patients with positive EV from cerebrospinal fluid (CSF) between 2014 and 2022, diagnosed at the Johns Hopkins Medical Microbiology Laboratory, were compared from year to year...
February 13, 2023: Journal of Clinical Virology
Evelyn Stelzl, Harald H Kessler, Amit D Parulekar, Carolin Bier, Dominik Nörz, Tanja Schneider, Suchitra Kumar, Christian O Simon, Marc Lütgehetmann
BACKGROUND: Regular screening for Epstein-Barr virus (EBV) DNA using quantitative RT-PCR is recommended for early intervention in at-risk patients. Harmonization of quantitative RT-PCR assays is critical to avoid misinterpretation of results. Here, we compare quantitative results of the cobas® EBV assay to four commercial RT-qPCR assays. METHODS: The cobas EBV, EBV R-Gene, artus EBV RG PCR, RealStar EBV PCR kit 2.0 and Abbott EBV RealTime assays were compared for analytic performance using a 10-fold dilution series of EBV reference material, normalized to the WHO standard...
February 11, 2023: Journal of Clinical Virology
Eloy E Ordaya, Paschalis Vergidis, Raymund R Razonable, Joseph D Yao, Elena Beam
BACKGROUND: Tixagevimab-cilgavimab is used for pre-exposure prophylaxis of COVID-19 in immunocompromised patients, though in vitro data has shown reduced neutralizing activity against SARS-CoV-2 Omicron subvariants. METHODS: We performed genomic sequencing of SARS-CoV-2 isolated from patients diagnosed with COVID-19 following tixagevimab-cilgavimab. Resistance-associated substitutions were used to generate a predicted phenotypic susceptibility analysis to tixagevimab-cilgavimab and bebtelovimab...
January 26, 2023: Journal of Clinical Virology
Runal John Steve, Arul Prakash, Suresh Ponnuvel, Calvin John Dickson, Karthick Nandan, Bakthalal Singh, Gift Ajay Sam, Ashish Goel, Uday George Zachariah, Chundamannil Eapen Eapen, Rajesh Kannangai, Priya Abraham, Gnanadurai John Fletcher
BACKGROUND: HBsAg Next assay (HBsAgNx) claims improved detection of HBsAg. The aim was to investigate its performance in ascertaining HBsAg loss, ability to detect HBsAg in various phases of HBV infection, specificity and its amenability to in-house neutralization. METHODS: Analytical sensitivity was investigated using NIBSC standard (3rd WHO-IS). For clinical performance, out of 91,962 samples tested for HBsAg (Qual-II), 512 samples consisting of 170 cases with evidence of HBsAg loss during treatment (n = 116) and without treatment (n = 54), acute-hepatitis B (n = 90) and acute exacerbation of chronic-hepatitis B (n = 41), acute-hepatitis A (n = 24) and acute-hepatitis E (n = 9) positive, HIV-1 positive (n = 20), non-HBV, HAV and HEV related acute-hepatitis (n = 81) and HBsAg prozone (n = 14) as well as in-house neutralization (n = 63) were included...
January 11, 2023: Journal of Clinical Virology
Amary Fall, Lijie Han, Omar Abdullah, Julie M Norton, Raghda E Eldesouki, Michael Forman, C Paul Morris, Eili Klein, Heba H Mostafa
BACKGROUND: An increase in influenza like illness in children and adolescents at the Johns Hopkins Health system during summer 2022 was associated with increased positivity for enterovirus/ rhinovirus. We sought to characterize the epidemiology and viral evolution of enterovirus D68 (EV-D68). METHODS: A cohort of remnant respiratory samples tested at the Johns Hopkins Microbiology Laboratory was screened for EV-D68. EV-D68 positives were characterized by whole genome sequencing and viral loads were assessed by droplet digital PCR (ddPCR)...
January 10, 2023: Journal of Clinical Virology
Marie-Laure Chaix, Nicolas Leturque, Audrey Gabassi, Isabelle Charreau, Marine Minier, Gilles Pialoux, Éric Cua, Christian Chidiac, François Raffi, Cécile Tremblay, Laurence Meyer, Jean-Michel Molina, Constance Delaugerre
BACKGROUND: Men who have sex with men (MSM) have an increased risk of infection by pathogens transmitted by the oro-fecal route. Here, we investigated the seroprevalence and incidence of hepatitis E virus (HEV) infection in 416 MSM included in the ANRS IPERGAY PrEP trial. RESULTS: Among the 62 (14.9% (95% CI: [11.6%-18.7%]) seropositive for HEV at inclusion, the only factor associated with testing seropositive for HEV was older age. Geographical origin, use of recreational drugs, number of sexual partners, status for HAV and bacterial sexually transmitted infection (STI) at inclusion were not associated...
January 9, 2023: Journal of Clinical Virology
Daiki Kanbayashi, Takako Kurata, Atsushi Kaida, Hideyuki Kubo, Seiji P Yamamoto, Kazutaka Egawa, Yuki Hirai, Kazuma Okada, Yuko Kaida, Ryo Ikemori, Takahiro Yumisashi, Ayami Ito, Takeshi Saito, Yoshihiko Yamaji, Yuka Nishino, Ryosuke Omori, Haruyo Mori, Kazushi Motomura, Kazuyoshi Ikuta
BACKGROUND: Since the first isolation of rubella virus (RuV) in 1962, comprehensive data regarding the quantitative evaluation of RuV shedding remain unavailable. In this study, we evaluated the shedding of viral RNA and infectious virus in patients with acute RuV infection. STUDY DESIGN: We analyzed 767 specimens, including serum/plasma, peripheral blood mononuclear cells (PBMCs), throat swabs, and urine, obtained from 251 patients with rubella. The viral RNA load and presence of infectious RuV were determined using reverse transcription-quantitative polymerase chain reaction (RT-qPCR) and virus isolation...
January 2, 2023: Journal of Clinical Virology
K Mourik, S A Boers, A L van Rijn, J C P Thijssen, E Wessels, E C J Claas
BACKGROUND: Viral load (VL) determination in patients with human immunodeficiency virus type 1 (HIV-1), hepatitis B virus (HBV) and hepatitis C virus (HCV) is essential for proper patient management and follow-up. New molecular platforms have been developed to fully automate these diagnostic assays. OBJECTIVE: Evaluation of the clinical performance of HIV-1, HBV and HCV VL assays on the Alinity m (Abbott) and NeuMoDx (Qiagen) molecular platforms. METHOD: Test panels of the three viruses have been compiled of 100 plasma and/or serum samples per target containing non-detectable, non-quantifiable and quantifiable VLs...
January 2, 2023: Journal of Clinical Virology
Ji-Man Kang, Juhan Lee, Kyu Ha Huh, Dong Jin Joo, Jae Geun Lee, Ha Yan Kim, Myeongjee Lee, Inkyung Jung, Min Young Kim, Sinyoung Kim, Younhee Park, Myoung Soo Kim
BACKGROUND: Solid organ transplant recipients (SOTRs) are susceptible to severe coronavirus disease 2019 (COVID-19); however, immunogenicity studies of the Omicron variants per vaccination schedules are still lacking. We examined humoral immunogenicity following third-dose mRNA vaccine administration in Korean SOTRs who received primary COVID-19 vaccine series on homologous or heterologous schedules. METHODS: We recruited SOTRs at Severance Hospital from October 27, 2021, to March 31, 2022...
December 30, 2022: Journal of Clinical Virology
Bryn K Joy, Alexis L Donovan, Gregory R McCracken, Janice Pettipas, Elsie Grudeski, Anna Majer, Russell Mandes, Tim F Booth, Todd F Hatchette, Glenn Patriquin, Jason J LeBlanc
BACKGROUND: Mpox (formerly monkeypox) is an emerging zoonotic disease of public health concern that presents as a rash mimicking other common viral exanthems. Unlike traditional testing algorithms relying on several assays, the BioFire FilmArray meningitis/encephalitis (ME) panel simultaneously detects common viruses causing rashes; however, Biofire ME is only licensed for testing on cerebral spinal fluid. OBJECTIVES: This study evaluated use of the Biofire ME panel for detection and discrimination of herpes simplex virus types 1 and 2 (HSV-1 and HSV-2), varicella zoster virus (VZV), human herpesviruses type 6 (HHV-6), enteroviruses (EVs), and human paraechoviruses (HPeVs) from a dermal or mucocutaneous swabs collected in universal transport media (UTM)...
December 25, 2022: Journal of Clinical Virology
Margaret G Mills, Kate B Juergens, Jolene P Gov, Carter J McCormick, Reigran Sampoleo, Alisa Kachikis, John K Amory, Ferric C Fang, Ailyn C Pérez-Osorio, Nicole A P Lieberman, Alexander L Greninger
BACKGROUND: In spring of 2022, an outbreak of monkeypox (mpox) spread worldwide. Here, we describe performance characteristics of monkeypox virus (MPXV)-specific and pan-orthopoxvirus qPCR assays for clinical use. METHODS: We validated probe-based qPCR assays targeting MPXV-specific loci F3L and G2R (genes MPXVgp052/OPG065 and MPXVgp002 and gp190/OPG002, respectively) and a pan-orthopoxvirus assay targeting the E9L locus (MPXVgp057/OPG071). Clinical samples and synthetic controls were extracted using the Roche MP96 or Promega Maxwell 48 instrument...
December 23, 2022: Journal of Clinical Virology
Brian R Lee, Anjana Sasidharan, Christopher J Harrison, Rangaraj Selvarangan
BACKGROUND: Enteroviruses (EV) and parechovirus (PeV) are a common cause of CNS infection in children. Both viruses demonstrate consistent seasonal patterns, with detections mainly in the summer-fall months. While research has shown COVID-19 pandemic-related disruption of traditional seasonality of respiratory pathogens, the pandemic's impact on non-respiratory pathogens is less understood. The aim of this study was to quantify the EV/PeV seasonal variations during pre-COVID years compared to variations observed during the COVID pandemic...
March 2023: Journal of Clinical Virology
Danny Ka-Ho Wong, Takako Inoue, Lung-Yi Mak, Rex Wan-Hin Hui, James Fung, Ka-Shing Cheung, Wai-Kay Seto, Yasuhito Tanaka, Man-Fung Yuen
BACKGROUND: This study aimed to evaluate the usefulness of two novel assays, namely the iTACT-hepatitis B surface antigen (iTACT-HBsAg) and iTACT-hepatitis B core-related antigen (iTACT-HBcrAg) assays, in chronic hepatitis B (CHB) patients with HBsAg seroclearance (SC) documented by standard assays. METHODS: HBsAg and HBcrAg were measured by the two iTACT-assays in 556 serial sera collected from 96 CHB patients at 7 different time points spanning from 5 years before to 10 years after SC and 120 HBsAg-negative, anti-HBc-positive individuals...
March 2023: Journal of Clinical Virology
Jasmine Coppens, Fien Vanroye, Isabel Brosius, Laurens Liesenborghs, Saskia van Henten, Thibaut Vanbaelen, Stefanie Bracke, Nicole Berens-Riha, Irith De Baetselier, Chris Kenyon, Patrick Soentjens, Eric Florence, Johan Van Griensven, Kevin K Ariën, Bart K M Jacobs, Dorien Van den Bossche, Marjan Van Esbroeck, Koen Vercauteren
BACKGROUND: Mpox (formerly monkeypox) is a viral disease caused by the mpox virus (MPXV), endemic in Central and West Africa and currently causing a global outbreak of international concern. Much remains unknown about sample types most suited for mpox laboratory diagnosis. While it is established that high viral loads can be found in active skin lesions (currently the recommended mpox laboratory confirmation specimen type), WHO mpox testing guidelines encourage the use of oropharyngeal swabs as an additional sample type for mpox diagnosis and suggest investigating the value of other specimens like blood samples...
February 2023: Journal of Clinical Virology
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