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Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology

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https://read.qxmd.com/read/30771597/the-detection-of-bkpyv-genotypes-ii-and-iv-after-renal-transplantation-as-a-simple-tool-for-risk-assessment-for-pyvan-and-transplant-outcome-already-at-early-stages-of-bkpyv-reactivation
#1
Johannes Korth, Olympia Evdoxia Anastasiou, Jan Hinrich Bräsen, Alexandra Brinkhoff, Ulrich Lehmann, Andreas Kribben, Ulf Dittmer, Jens Verheyen, Benjamin Wilde, Sandra Ciesek, Oliver Witzke, Marek Widera
BACKGROUND: After reactivation the BK-polyomavirus (BKPyV) associated nephropathy (PyVAN) is observed in 1-10% of renal transplant recipients, of which up to 80% undergo graft failure. BKPyV reactivation after renal transplantation was associated with donor-derived serotypes against which the recipient has no immunological protection. However, PyVAN risk assessment seroactivity testing is a time-consuming and cost intensive process. OBJECTIVES: Since BKPyV serotypes can be attributed to distinct genotypes I to IV, in the present study we retrospectively analyzed whether a simple PCR-based BKPyV genotyping assay might be a fast and inexpensive method to assess the risk for PyVAN and transplant outcome already at early stages of BKPyV reactivation...
February 10, 2019: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://read.qxmd.com/read/30763816/intrahost-viral-evolution-during-chronic-sapovirus-infections
#2
Corinna Pietsch, Uwe G Liebert
BACKGROUND: Sapovirus is a common cause of self-limiting diarrhea. In immunocompromised individuals chronic infections occur, but are incompletely investigated. OBJECTIVES: To investigate viral evolution in immunocompromised hosts during chronic sapovirus infection. STUDY DESIGN: From January 2010 to September 2018 stool samples of 5333 in-patients were analyzed for the presence of sapovirus RNA by real-time RT-PCR. In follow-up samples of chronic diarrhea cases nucleic acid sequencing of sapovirus genomes was performed...
February 7, 2019: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://read.qxmd.com/read/30771598/a-pan-genotypic-hepatitis-c-virus-ns5a-amplification-method-for-reliable-genotyping-and-resistance-testing
#3
Andreas Walker, Kim Sophie Ennker, Rolf Kaiser, Nadine Lübke, Jörg Timm
BACKGROUND: Chronic infection with the Hepatitis C Virus (HCV) is associated with the risk of progressive liver disease. Although, HCV treatment options and viral cure rates have tremendously increased over the last decade, all currently licensed combination therapies contain inhibitors of the replication complex NS5A. Resistance-associated substitutions (RAS) in NS5A can limit the efficacy of therapy; however, resistance testing is routinely not recommended for all patients. Notably, pan-genotypic combinations have been approved, however the correct identification of the HCV genotype is still required for treatment decisions and is a good predictor for treatment success...
January 30, 2019: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://read.qxmd.com/read/30738366/evaluation-of-a-new-rapid-fluorescence-immunoassay-for-the-diagnosis-of-dengue-and-zika-virus-infection
#4
Lorenzo Zammarchi, Maria Grazia Colao, Antonia Mantella, Teresa Capobianco, Gianna Mazzarelli, Nunziata Ciccone, Seble Tekle Kiros, Elisabetta Mantengoli, Gian Maria Rossolini, Alessandro Bartoloni
BACKGROUND: Dengue (DENV) and Zika virus (ZIKV) are important mosquito-transmitted viruses. OBJECTIVES: To investigate the performance of Standard F, Fluorescence Immunoassay (FIA, SD Biosensor Inc., Suwon, South Korea) providing results in 15 min to detect DENV IgG, IgM and NS1Ag, and ZIKV IgG, IgM, and Ag. STUDY DESIGN: A well-characterized panel of patient samples (11 acute DENV, 11 acute ZIKV, 10 past DENV, 10 past ZIKV infection, 36 with other conditions) were tested with the FIA test...
January 29, 2019: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://read.qxmd.com/read/30776575/performance-evaluation-of-the-hologic-aptima-hcv-quant-dx-assay-for-detection-of-hcv-rna-from-dried-blood-spots
#5
Beth Catlett, Alex Carrera, Mitchell Starr, Tanya L Applegate, Peter Lowe, H Philip Cunningham, Jason Grebely
BACKGROUND: The availability of effective direct-acting antiviral therapy for hepatitis C virus (HCV) has led to a need for simplified diagnostic pathways. Barriers to treatment uptake, specifically in people who inject drugs and in remote and resource limited settings, may be overcome by utilizing novel collection methods, such as dried blood spots (DBS). However, there are currently no registered assays for HCV RNA testing from DBS samples. OBJECTIVES: To evaluate the sensitivity and specificity of the Aptima HCV Dx Quant assay for HCV RNA detection in DBS samples STUDY DESIGN: 107 paired venepuncture and DBS samples from HCV antibody positive individuals were analyzed for HCV RNA on the Aptima HCV Dx Quant and Roche CAP/CTM (gold standard) HCV assays...
January 25, 2019: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://read.qxmd.com/read/30711798/determination-of-rubella-virus-specific-humoral-and-cell-mediated-immunity-in-pregnant-women-with-negative-or-equivocal-rubella-specific-igg-in-routine-screening
#6
O Picone, E Bouthry, Y Bejaoui-Olhmann, A G Cordier, S Nedellec, A Letourneau, M Carbonel, M Brollo, L Grangeot-Keros, J M Ayoubi, A Benachi, E Rouge, C Vauloup-Fellous
BACKGROUND: Immunity to rubella-virus (RV) is commonly determined by measuring specific IgG (RV-IgG). However, RV-IgG results may be different and even discordant, depending on the assay used. Cell-mediated immunity is not routinely investigated for diagnostic purposes. OBJECTIVES: Our aim was to investigate humoral and cellular immunity of women with negative or equivocal RV-IgG before, and after post-partum vaccination. STUDY DESIGN: A total of 186 pregnant women were included in the study...
January 24, 2019: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://read.qxmd.com/read/30708281/high-epidemic-burden-of-rsv-disease-coinciding-with-genetic-alterations-causing-amino-acid-substitutions-in-the-rsv-g-protein-during-the-2016-2017-season-in-the-netherlands
#7
Laura M Vos, Jan Jelrik Oosterheert, Sacha D Kuil, Marco Viveen, Louis J Bont, Andy I M Hoepelman, Frank E J Coenjaerts
BACKGROUND: We found amino acid substitutions in the Gglycoprotein of respiratory syncytial virus (RSV) A during the 2016/2017 epidemic in The Netherlands. OBJECTIVES: We evaluated whether these alterations led to increased RSV incidence and disease burden. STUDY DESIGN: We sequenced the gene encoding the G-protein of prospectively collected clinical specimens from secondary care adult patients testing positive for RSV during the 2016/2017 and 2017/2018 epidemic RSV season...
January 23, 2019: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://read.qxmd.com/read/30669024/human-metapneumovirus-in-paediatric-intensive-care-unit-picu-admissions-in-the-united-kingdom-uk-2006-2014
#8
Rachael Barr, Rachel McGalliard, Simon B Drysdale
BACKGROUND: Human metapneumovirus (HMPV) is a pneumovirus known to cause respiratory disease in children. It was identified as a pathogen in 2001 and its healthcare burden and associated costs are not fully understood. OBJECTIVES: This study aimed to assess the clinical characteristics of children with HMPV infection admitted to paediatric intensive care units (PICUs) across the United Kingdom (UK) over a nine-year period and to estimate the associated costs of care...
January 16, 2019: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://read.qxmd.com/read/30658273/performance-characteristics-of-the-vidas%C3%A2-anti-hev-igm-and-igg-assays
#9
Florence Abravanel, Nadège Goutagny, Raphaelle Joffray, Emile Eichenlaub, Sylviane Baron, Alexandre Aversenq, Sandrine Bourg, Laurence Mercier, Audrey Larue Triolet, Didier Poirault, Marissa Loubet, Soizic Daniel, Francoise Luciani, Catherine Pothion, Carole Tourneur, Jean-Marc Dugua, Sébastien Lhomme, Jacques Izopet
BACKGROUND: Several unautomated anti-HEV diagnostic tests are presently available. OBJECTIVE: We have evaluated the performance of the new automated VIDAS® ANTI-HEV IgM and IgG assays. STUDY DESIGN: We assessed the reproducibility and cross-reactivity of both VIDAS assays and the analytical sensitivity and linearity of the VIDAS IgG assay. We also tested the VIDAS and comparator assays Wantai IgG and IgM on immunocompetent and immunocompromised patients...
January 11, 2019: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://read.qxmd.com/read/30654207/challenges-in-management-of-epidemic-keratoconjunctivitis-with-emerging-recombinant-human-adenoviruses
#10
REVIEW
Gabriel Gonzalez, Nobuyo Yawata, Koki Aoki, Nobuyoshi Kitaichi
Adenoviral epidemic keratoconjunctivitis (EKC) presents as severe conjunctival inflammations involving the cornea that can lead to the development of corneal opacities and blurred vision, which can persist for months. EKC is highly contagious and responsible for outbreaks worldwide, therefore accurate diagnosis and rapid containment are imperative. EKC is caused by a number of types within Human adenovirus species D (HAdV-D): 8, 37 and 64 (formerly known as 19a) and these types were considered the major causes of EKC for over fifty years...
January 11, 2019: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://read.qxmd.com/read/30639845/performance-of-four-diagnostic-assays-for-detecting-herpes-simplex-virus-type-2-antibodies-in-the-middle-east-and-north-africa
#11
Gheyath K Nasrallah, Soha R Dargham, Afifah S Sahara, Malaz S Elsidiq, Laith J Abu-Raddad
BACKGROUND: Assessments of commercial assays in detecting herpes simplex virus type 2 (HSV-2) antibodies have shown variable sensitivity and specificity, and variation in performance by global population. OBJECTIVE: To evaluate performance of four assays in detecting HSV-2 antibodies in a composite Middle Eastern and North African (MENA) population. The assays are two ELISA kits: HerpeSelect® 2 ELISA IgG and Euroimmun Anti-HSV-2 (gG2) ELISA (IgG), and two immunoblot (IB)/Western blot (WB) assays: HerpeSelect® 1 and 2 Immunoblot IgG and Euroimmun Anti-HSV-1/HSV-2 gG2 Euroline-WB (IgG/IgM)...
January 7, 2019: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://read.qxmd.com/read/30639844/prospective-evaluation-of-three-rapid-molecular-tests-for-seasonal-influenza-in-patients-presenting-at-an-emergency-unit
#12
Thomas Valentin, Petra Kieslinger, Evelyn Stelzl, Brigitte I Santner, Andrea Groselj-Strele, Harald H Kessler, Beate Tiran
BACKGROUND: For infection control measures, rapid accurate diagnostics on admission of patients with suspected seasonal influenza is crucial. OBJECTIVE: Prospective comparison of three rapid molecular tests for detection of influenza A/B RNA. STUDY DESIGN: Outpatients presenting at the Medical emergency department of Graz University Hospital with influenza-like illness and a requirement for hospitalization (n = 312) were studied. Nasopharyngeal swabs were collected with the 3 mL-version of the UTM™ Viral Transport Medium (Copan)...
January 7, 2019: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://read.qxmd.com/read/30639846/demonstration-of-the-diagnostic-agreement-of-capillary-and-venous-blood-samples-using-hepatitis-c-virus-sd-bioline-%C3%A2-rapid-test-a-clinic-based-study
#13
Chhorvy Sun, Momoko Iwamoto, Aurelie Calzia, Bun Sreng, Sokchea Yann, Sorphorn Pin, Celine Lastrucci, San Kimchamroeun, Chhit Dimanche, Jean-Philippe Dousset, Mickael Le Paih, Suna Balkan, Tonia Marquardt, Valentina Carnimeo, Pascale Lissouba, David Maman, Anne Loarec
BACKGROUND: Simplifying hepatitis C virus (HCV) screening is a key step in achieving the elimination of HCV as a global public health threat by 2030. OBJECTIVES: The objective of this study was to demonstrate the agreement of capillary blood and venipuncture specimens when using SD Bioline© HCV, a low-cost rapid diagnostic test (RDT), prequalified by WHO in 2016 on venous blood samples. STUDY DESIGN: Recruitment was conducted prospectively among adult patients presenting for HCV testing at the Médecins Sans Frontières (MSF) clinic of Preah Kossamak Hospital (Phnom Penh, Cambodia) between October and November 2017...
December 31, 2018: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://read.qxmd.com/read/30594702/performance-evaluation-of-the-bio-rad-geenius-hiv-1-2-supplemental-assay
#14
Wei Luo, Vickie Sullivan, Tara Smith, Philip J Peters, Cynthia Gay, Emily Westheimer, Stephanie E Cohen, S Michele Owen, Silvina Masciotra
BACKGROUND: In the US, the HIV diagnostic algorithm for laboratory settings recommends the use of an HIV-1/HIV-2 differentiation supplemental assay after an initial reactive antigen/antibody (Ag/Ab) assay result. Since the discontinuation of the Multispot HIV-1/HIV-2 Rapid Test (MS), the Geenius HIV-1/2 Supplemental assay (Geenius) is the only FDA-approved supplemental differentiation test. OBJECTIVE: We compared the performance of Geenius to MS and Western Blot (WB)...
December 19, 2018: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://read.qxmd.com/read/30594700/performance-of-geno2pheno-coreceptor-to-infer-coreceptor-use-in-human-immunodeficiency-virus-type-1-hiv-1-subtype-a
#15
Ilaria Vicenti, Alessia Lai, Alessia Giannini, Adele Boccuto, Filippo Dragoni, Francesco Saladini, Maurizio Zazzi
BACKGROUND: Assessment of human immunodeficiency virus type 1 (HIV-1) coreceptor usage is required prior to treatment with the CCR5 antagonist maraviroc to exclude the presence of CXCR4-using (X4) strains. Genotype-based interpretation systems are mostly designed on subtype B and have been reported to be less accurate for subtype A/CRF02_AG. OBJECTIVES: To evaluate the performance of the widely used Geno2Pheno[coreceptor] (G2P[c]) algorithm for prediction of coreceptor usage with subtype A/CRF02_AG vs...
December 19, 2018: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://read.qxmd.com/read/30594701/human-adenovirus-in-nasopharyngeal-and-blood-samples-from-children-with-and-without-respiratory-tract-infections
#16
Hans-Johnny Schjelderup Nilsen, Svein Arne Nordbø, Sidsel Krokstad, Henrik Døllner, Andreas Christensen
BACKGROUND: Human adenovirus (HAdV) is a double-stranded DNA virus associated with respiratory tract infections (RTI) in children. Using polymerase chain reaction (PCR) tests, HAdV often is detected together with other virus species, even in healthy controls. OBJECTIVES: The aim of this study was to compare molecular detection of HAdV with culture, and to examine the associations of various methods to RTI. STUDY DESIGN: Nasopharyngeal aspirates (NPA) were collected from 4319 children admitted with RTI and from 361 controls...
December 18, 2018: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://read.qxmd.com/read/30580015/viral-gastroenteritis-among-children-of-0-5-years-in-nigeria-characterization-of-the-first-nigerian-aichivirus-recombinant-noroviruses-and-detection-of-a-zoonotic-astrovirus
#17
M O Japhet, O Famurewa, O A Adesina, O O Opaleye, B Wang, M Höhne, C T Bock, Andreas Mas Marques, S Niendorf
BACKGROUND: Viruses are the leading cause of acute gastroenteritis in children worldwide. Understanding of the occurrence and genetic diversity of these viruses can help to prevent infections. OBJECTIVES: The present study describes the presence, genetic diversity and possible recombination of five enteric viruses in children with gastroenteritis in Southwestern Nigeria. STUDY DESIGN: From August 2012 to December 2013, stool samples and sociodemographic data of 103 diarrheic children <5 years were collected to detect and characterize rotavirus A, norovirus, human astrovirus, aichivirus and sapovirus using PCR techniques followed by sequencing and phylogenetic analyses...
December 17, 2018: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://read.qxmd.com/read/30579036/performance-evaluation-of-the-re-standardized-abbott-architect-anti-hbs-assay
#18
Ann H Wong, Yolanda I I Ho, Raymond W M Lai
BACKGROUND: As defined by World Health Organization (WHO), an antibody level of ≥ 10mIU/mL to hepatitis B virus confers protection. With the launching of Abbott anti-HBs assay re-standardized to the 2nd WHO International Reference Preparation, a positive bias in antibody level would be anticipated. Manufacturer provides limited data for samples around the immune cut-off which has potential implication on vaccine guidance. OBJECTIVES: To evaluate the performance of the re-standardized Abbott Architect anti-HBs assay and to determine the impact of the upward shift...
December 15, 2018: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://read.qxmd.com/read/30530285/problematic-molecular-diagnosis-of-hsv-1-infection-due-to-a-single-nucleotide-polymorphism-in-the-us7-gene
#19
Sébastien Vitale, Céline Loubatier, Isabelle Cannavo, Valérie Giordanengo
BACKGROUND: HSV-1 infection is very common worldwide and can be associated with severe medical conditions such as encephalitis and neonatal herpes. Current diagnosis relies heavily on molecular assays targeting the viral genome. One of the pitfalls of these assays is genetic variability, as viral polymorphisms located in the target region can impair the detection of HSV-1. OBJECTIVES: The aim of this study was to determine if genetic diversity of HSV-1 could account for equivocal assay results we obtained during routine diagnosis of HSV-1 in a genital specimen with the HSV-1 HSV-2 VZV R-gene® assay...
December 3, 2018: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://read.qxmd.com/read/30530097/comparative-performance-of-the-biocentric-generic-viral-load-roche-cap-ctm-v1-5-roche-cap-ctm-v2-0-and-m2000-abbott-assays-for-quantifying-hiv-1-b-and-non-b-strains-underestimation-of-some-crf02-strains
#20
V Avettand-Fénoël, A Mélard, M Gueudin, A Maillard, J Dina, M Gousset, M L Chaix, N Lerolle, J P Viard, L Meyer, J C Plantier, C Rouzioux
BACKGROUND: HIV-1 viral load testing is now recommended by the World Health Organization for every patient receiving antiretroviral therapy (ART). OBJECTIVES: The objective of this study is to evaluate the performance of commercial assays for their ability to quantify HIV-1 strains currently circulating in France. STUDY DESIGN: The performances of the Generic HIV-RNA assay from Biocentric were compared to those of the Roche CAP/CTM v1.5, Roche CAP/CTM v2...
December 3, 2018: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
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