Downregulation of lncRNAs Gomafu, NONMMUT033604.2, and NONMMUT064397.2 in the hippocampus of mice with model of post-traumatic stress disorder.

Objectives. Molecular mechanisms of post-traumatic stress disorder (PTSD) development have been analyzed by evaluati-ng changes in the expression level of long non-coding RNA (lncRNA) as a potential biomarker of the disease and as one of the molecular aspects associated with the disease development. Methods. In our study, we used quantitative polymerase chain reaction (qPCR) to evaluate changes in the expression level of long non-coding RNA - Gomafu, NONMMUT033604.2, and NONMMUT064397.2 - in the hippocampus of mice that were subjected to an artificially induced middle single prolonged stress (mSPS) model of post-traumatic stress disorder. Results. We found a significant reduction in the expression levels of each of the three lncRNAs tested: Gomafu in 45,4 times, NONMMUT033604.2 in 53,4 times, and NONMMUT064397.2 in 5,2 times. The results of the present study provide evidence that mSPS model effectively induces PTSD-like behavior in mice leading to a significant decrease in the expression level of Gomafu, NONMMUT033604.2 and NONMMUT064397.2 lncRNA in mice hippocampus. Conclusions. This data provides evidence that the three studied lncRNAs could be potential biomarkers of PTSD development.