Preparing and evaluating the anti-microbial effect of Allium jesdianum mouthwash on some of the most common oral microorganisms.

BACKGROUND: Due to the increasing resistance of bacteria to antibiotics and anti-bacterial compounds in plants, Allium jesdianum Boiss plant extract can be used in mouthwash compounds with its anti-microbial activity.

METHODS AND MATERIALS: The anti-bacterial and anti-fungal activity of A. jesdianum mouthwash was investigated on Streptococcus mutans , Streptococcus sanguis , S. salivarius and Candida albicans , and Candida tropicalis . To analyse the anti-microbial effect of this mouthwash, minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were determined by the broth microdilution method.

RESULTS: The average MIC and MBC of A. jesdianum mouthwash for S. mutans were 1.56 and 3.12 (mg/ml), respectively, for S. salivarius , 0.25 and 0.65 (mg/ml), and for S. sanguis , respectively, 0.25 and 0.65 (mg/ml). The highest MIC and MBC values were for S. mutans , and the MIC and MBC values were equal for S. sanguis and S. salivarius . Average MIC and MBC were determined as 2.41 and 4.16 (mg/ml) for C. albicans and 2.34 and 5.72 (mg/ml) for C. tropicalis , respectively. MIC values of mouthwash were higher for C. albicans and MBC values for C. tropicalis .

CONCLUSION: Our results showed a promising anti-fungal-anti-bacterial effect of A. jesdianum extract. A. jesdianum extract may be used as an alternative to chemical mouthwashes.