Fractionation and identification of bioactive compounds from a salt marsh plant Sesuvium potrucastrulam (L.) and its antioxidant activity.

Sesuvium portulacastrum (L.) is a fast-growing herbaceous perennial and halophyte belonging to the family Aizoaceae. Bioactive compound identification from halophytes is more helpful for the drug development process. The present investigation was to fractionate and identify the bioactive compounds of leaf extracts from Sesuvium potrucastrulam and evaluate their antioxidant potential. The Soxhlet extraction method was used in this study, and column chromatography was done for the partial purification. The bactericidal activity of the fraction was determined using the agar-well diffusion technique, and the effective fraction was analysed by GC-MS. A hydrogen peroxide-reducing assay was carried out on the antioxidant activity of the elite fraction. Five active fractions were collected from the crude extract. Fraction (F3) exhibited promising antibacterial and antioxidant activity. GS-MS analysis suggested the active compounds of the elite fraction are n-Hexadecanoic acid (29.70%), oleic acid (8.08%), octadecatrienoic acid (8.01%), 2-methoxy-4-vinylphenol (6.65%), tertracosamethyl-cyclododecasiloxane (6.55%), and lineolic acid (4.68%).