The H 2 O 2 Self-sufficient 3D Printed β-TCP Scaffolds with Synergistic Anti-tumor Effect and Reinforced Osseointegration.

Tumor recurrence and massive bone defects are two critical challenges for postoperative treatment of oral and maxillofacial tumor, posing serious threats to the health of patients. Herein, in order to eliminate residual tumor cells and promote osteogenesis simultaneously, the hydrogen peroxide (H2 O2 ) self-sufficient TCP-PDA-CaO2 -CeO2 (TPCC) scaffolds are designed by preparing CaO2 or/and CeO2 nanoparticles (NPs)/chitosan solution and modifying the NPs into polydopamine (PDA)-modified 3D printed TCP scaffolds by rotary coating method. CaO2 NPs loaded on the scaffolds could release Ca2+ and sufficient H2 O2 in the acidic tumor microenvironment. The generated H2 O2 could further produce hydroxyl radicals (·OH) under catalysis effect by peroxidase (POD) of CeO2 NPs, in which the photothermal effect of the PDA coating enhances its POD catalytic effect. Overall, NPs loaded on the scaffold chemically achieve a cascade reaction of H2 O2 self-sufficiency and ·OH production, while functionally achieving synergistic effects on anti-tumor and bone promotion. In vitro and in vivo studies showed that the scaffolds exhibited effective osteo-inductivity, induced osteoblast differentiation and promoted osseointegration. Therefore, the multifunctional composite scaffolds not only validate the concept of chemo-dynamic therapy (CDT) cascade therapy, but also provide a promising clinical strategy for postoperative treatment of oral and maxillofacial tumor. This article is protected by copyright. All rights reserved.