Anti-Inflammatory Effect of Dental Pulpa Mesenchymal Stem Cell Exosomes Loaded Mucoadhesive Hydrogel on Mice with Dental Nickel Hypersensitivity.

In this study, the anti-inflammatory effect of dental pulp MSC exosomes loaded to mucoadhesive hydrogel was investigated in a dental nickel hypersensitivity murine model. After culture of dental pulp MSCs in the third passage MSCs were loaded to a mucoadhesive hydrogel based on chitosan, cross-linked with genipin and modified with catechol groups. A dental nickel hypersensitivity model was created by administering NiCl2 and 10 μg/mL LPS to 4-6-week-old Balb-c mice by intradermal injection. In mice treated with dental pulp MSC exosomes and exosomes in hydrogel, IFN-γ secreting CD4+T lymphocyte ratios significantly increased compared to the untreated group (p<0.05). IFN-γ and IL-10 expression in buccal mucosa tissue sections and IFN-γ secreting CD4+T lymphocyte ratios were found to be significantly higher in mice treated with DPMKH exosomes and DPMKH exosomes in hydrogel compared to the untreated group (p<0.05). According to flow cytometry results, IL-4 secreting CD4+T lymphocytes were found to be significantly decreased in DPMKH exosomes group compared to dental nickel hypersensitivity group (p<0.05). Administration of DPMKH exosomes with mucoadhesive hydrogel may be an alternative to current medication in the treatment of dental nickel hypersensitivity. This article is protected by copyright. All rights reserved.