Ultrasensitive biosensing of thiram based on detection of the DNA damage induced by thiram: Application to investigation of protective effects of extra virgin olive oil against DNA damage.

In this work, a novel electrochemical biosensor was fabricated based on modification of a glassy carbon electrode (GCE) with nafion-DNA/gold nanoparticles/poly-ethylenedioxy pyrrole/multi-walled carbon nanotubes-ionic liquid (NF-DNA/Au NPs/PEDOP/MWCNTs-IL/GCE) with the aim of amperometric detection of the DNA damage induced by thiram (TH). By incubation of the biosensor with the TH, the TH was intercalated within DNA, and the exposed DNA released negative charges at the surface of the biosensor which repelled the probe molecules and caused the amperometric response of the biosensor to be decreased. Protective effects of extra virgin olive oil (EVOO) on the DNA damage induced by the TH were investigated by recording amperometric responses of the biosensor in the presence of EVOO, and the results confirmed that the response of the biosensor didn't change to confirm the protective effects of the EVOO on preventing the DNA damage induced by the TH. A novel and sensitive electroanalytical method was developed for determination of the TH in two linear ranges including 1-6 pM and 7-10 pM based on amperometric detection of the DNA damage induced by the TH which gave a LOD of 0.31 pM. The developed methodology in this work was successful in detection of the DNA damage induced by TH, detection of protective effects of EVOO on preventing DNA damage and determination of the TH in real matrices.