Guided filter based image enhancement for focal error compensation in low cost automated histopathology microscopic system.

Low-cost automated histopathology microscopy systems usually suffer from optical imperfections, producing images that are slightly Out of Focus (OoF). In this work, a guided filter (GF) based image preprocessing is proposed for compensating focal errors and its efficacy is demonstrated on images of healthy and malaria infected red blood cells (h-RBCs and i-RBCs), and PAP smears. Since contrast enhancement has been widely used as an image preprocessing step for the analysis of histopathology images, a systematic comparison is made with six such prominently used methods, namely Contrast Limited Adaptive Histogram Equalization (CLAHE), RIQMC-based optimal histogram matching (ROHIM), modified L0 , Morphological Varying(MV)-Bitonic filter, unsharp mask filter and joint bilateral filter. The images enhanced using GF approach lead to better segmentation accuracy (upto 50% improvement over native images) and visual quality compared to other approaches, without any change in the color tones. Thus, the proposed GF approach is a viable solution for rectifying the OoF microscopy images without the loss of the valuable diagnostic information presented by the color tone.