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Journal of Biophotonics

Shu Zhang, Lachlan J Gibson, Alexander B Stilgoe, Timo A Nieminen, Halina Rubinsztein-Dunlop
Exploring the rheological properties of intracellular materials is essential for understanding cellular and subcellular processes. Optical traps have been widely used for physical manipulation of micro- and nano-objects within fluids enabling studies of biological systems. However, experiments remain challenging as it is unclear how the probe particle's mobility is influenced by the nearby membranes and organelles. We use liposomes (unilamellar lipid vesicles) as a simple biomimetic model of living cells, together with a trapped particle rotated by optical tweezers to study mechanical and rheological properties inside a liposome both theoretically and experimentally...
February 18, 2019: Journal of Biophotonics
Tian Yao, Runyu Cao, Wen Xiao, Feng Pan, Xiaoping Li
Platinum chemo-sensitivity detection plays a vital role during endometrial cancer treatment because chemotherapy responses have profound influences on patient's prognosis. Although several methods can be used to detect drug resistance characteristics, studies on detecting drug sensitivity based on dynamic and quantitative phase imaging of cancer cells are rare. In this study, digital holographic microscopy was applied to distinguish drug-resistant and non-drug-resistant endometrial cancer cells. Based on the reconstructed phase images, temporal evolutions of cell height, cell projected area and cell volume were quantitatively measured...
February 15, 2019: Journal of Biophotonics
Ning Ding, Xiaoxiao Liu, Ningbo Chen, Jing Jiang, Huangxuan Zhao, Zhigang Li, Jianhui Zhang, Chengbo Liu
As a stimulating point in acupuncture, acupoint has unique microcirculatory features and its dynamics vary greatly depending on health status. Acupoint sensitization is defined as the transformation of an acupoint from a "silenced status" (healthy) to an "activated status" (disease). Our previous study demonstrated that acupoint sensitization is associated with an increase in the level of local blood perfusion. However, the structural changes in microcirculation during acupoint sensitization have yet to be elucidated since the high-resolution microcirculation imaging of acupoints has been difficult to obtain...
February 10, 2019: Journal of Biophotonics
Qian Zhao, Riqiang Lin, Chengbo Liu, Jingxiu Zhao, Guangtao Si, Liang Song, Jing Meng
Optical-resolution photoacoustic microscopy (OR-PAM) has been shown to be an excellent imaging modality for monitoring and study of tumor microvasculature. However, previous studies focused mainly on the normal tissues and did not quantify the tumor microvasculature. In this study, we present an in vivo OR-PAM imaging of the melanomas and hepatoma implanted in the mouse ear. We quantify the vessel growth by extracting the skeletons of both dense and thin branches of the tumor microvasculature obtained by Hessian matrix enhancement followed by improved two-step multistencils fast marching method...
February 8, 2019: Journal of Biophotonics
Sungbea Ban, Nam Hyun Cho, Eunjung Min, Jung Kweon Bae, Yujin Ahn, Sungwon Shin, Soo-Ah Park, Yoonsung Lee, Woonggyu Jung
For many decades, mammalian embryo has been widely used as a key model to recapitulate human development process and congenital disease due to its biological similarity and accessibility. To understand the development of specific tissues or potentially involved in specific disease symptoms, diverse genetic experimental approaches including knock-out mouse system have been developed. This article is protected by copyright. All rights reserved.
February 7, 2019: Journal of Biophotonics
L N Hu, W S Li, H Y Lin, Y Li, H Zhang, K Svanberg, S Svanberg
An improved method, where conventional otoscope investigation of human suspicious otitis media is combined with diffuse reflectance spectroscopy and gas in scattering media absorption spectroscopy (GASMAS) is being developed. Otitis media is one of the most common infectious diseases in children, whose Eustachian tube connecting the middle ear with the nasal cavity is more horizontal than for adults, which leads to impaired fluid drainage. At present, the use of an otoscope to visually observe possible changes in the tympanic membrane appearance is the main diagnostics method for otitis media...
February 5, 2019: Journal of Biophotonics
Aditi Sahu, Poonam Gera, Akshat Malik, Sudhir Nair, Pankaj Chaturvedi, C Murali Krishna
Oral cancer is associated with high rates of recurrence attributable to field cancerization. Early detection of advanced field changes that can potentially progress to carcinoma can facilitate timely intervention and can lead to improved prognosis. Previous in vivo studies have successfully detected advanced field effects in oral cancers. Raman exfoliative cytology has previously shown to differentiate normal, oral pre-cancer and cancers. The present study explores Raman-exfoliative-cytology-based detection of field effects...
February 5, 2019: Journal of Biophotonics
Kelvin C M Lee, Andy K S Lau, Anson H L Tang, Maolin Wang, Aaron T Y Mok, Bob M F Chung, Wenwei Yan, Ho Cheung Shum, Kathryn S E Cheah, Godfrey C F Chan, Hayden K H So, Kenneth K Y Wong, Kevin K Tsia
A growing body of evidence has substantiated the significance of quantitative phase imaging (QPI) in enabling cost-effective and label-free cellular assays, which provide useful insights into understanding biophysical properties of cells and their roles in cellular functions. However, available QPI modalities are limited by the loss of imaging resolution at high throughput and thus run short of sufficient statistical power at the single cell precision to define cell identities in a large and heterogeneous population of cells - hindering their utility in mainstream biomedicine and biology...
February 4, 2019: Journal of Biophotonics
Kavon Karrobi, Anup Tank, Syeda Tabassum, Vivian Pera, Darren Roblyer
Diffuse Optical Imaging (DOI) techniques provide a wide-field or macro assessment of the functional tumor state, and have shown substantial promise for monitoring treatment efficacy in cancer. Conversely, intravital microscopy provides a high-resolution view of the tumor state, and has played a key role in characterizing treatment response in the preclinical setting. There has been little prior work in investigating how the macro and micro spatial scales can be combined to develop a more comprehensive and translational view of treatment response...
January 31, 2019: Journal of Biophotonics
Yang Zhao, Kengyeh K Chu, Evan T Jelly, Adam Wax
Recent studies have demonstrated that extended imaging depth can be achieved using dual-axis optical coherence tomography (DA-OCT). By illuminating and collecting at an oblique angle, multiply forward scattered photons from large probing depths are preferentially detected. However, the mechanism behind the enhancement of imaging depth needs further illumination. Here, the signal of a DA-OCT system is studied using a Monte Carlo (MC) simulation. We modeled light transport in tissue and recorded the spatial and angular distribution of photons exiting the tissue surface...
January 30, 2019: Journal of Biophotonics
H Serrage, S Joanisse, P R Cooper, W Palin, M Hadis, O Darch, A Philp, M R Milward
OBJECTIVE: Photobiomodulation (PBM) is the application of light to promote tissue healing. Current indications suggest PBM induces its beneficial effects in vivo through upregulation of mitochondrial activity. However, how mitochondrial content influences such PBM responses has yet to be evaluated. Hence, the current study assessed the biological response of cells to PBM with varying mitochondrial contents. METHODS: DNA was isolated from myoblasts and myotubes (differentiated myoblasts) and mitochondrial DNA (mtDNA) was amplified and quantified using a microplate assay...
January 30, 2019: Journal of Biophotonics
Ben Mc Larney, Johannes Rebling, Zhenyue Chen, Xosé Luis Deán-Ben, Sven Gottschalk, Daniel Razansky
Accurate image reconstruction in volumetric optoacoustic tomography implies the efficient generation and collection of ultrasound signals around the imaged object. Non-uniform delivery of the excitation light is a common problem in optoacoustic imaging often leading to a diminished field of view, limited dynamic range and penetration, as well as impaired quantification abilities. Presented here is an optimised illumination concept for volumetric tomography that utilizes additive manufacturing via 3D printing in combination with custom-made optical fiber illumination...
January 30, 2019: Journal of Biophotonics
Jian Ju, Sagar Regmi, Afu Fu, Sierin Lim, Quan Liu
Graphene quantum dots (GQD), the new zero-dimensional carbon nanomaterial, has been demonstrated as a promising material for biomedical applications due to its good biocompatibility and low toxicity. However, the integration of multiple therapeutic approaches into a nano-sized platform based on the GQD has not been explored yet to our best knowledge. In this report, we regulate the generation of reactive oxygen species (ROS) when using the GQD as a photosensitizer by varying the doping amount of nitrogen atoms to achieve efficiency controllable photodynamic therapy (PDT)...
January 30, 2019: Journal of Biophotonics
Ondřej Podrazký, Pavel Peterka, Ivan Kašík, Soňa Vytykáčová, Jana Proboštová, Jan Mrázek, Martin Kuneš, Veronika Závalová, Věra Radochová, Oleksiy Lyutakov, Edoardo Ceci-Ginistrelli, Diego Pugliese, Nadia G Boetti, Davide Janner, Daniel Milanese
Optical fibers have recently attracted a noticeable interest for biomedical applications since they provide a minimally invasive method for in-vivo sensing, imaging techniques, deep-tissue photodynamic therapy or optogenetics. The silica optical fibers are the most commonly used because they offer excellent optical properties and they are readily available at a reasonable price. The fused silica is a biocompatible material, but it is not bioresorbable so it does not decompose in the body and the fibers must be ex-planted after in-vivo use and their fragments can present a considerable risk to the patient when the fiber breaks...
January 29, 2019: Journal of Biophotonics
Laura M G van Huizen, Nikolay V Kuzmin, Ellis Barbé, Susanne van der Velde, Elisabeth A Te Velde, Marie Louise Groot
Real-time assessment of excised tissue may help to improve surgical results in breast tumor surgeries. Here, as a step towards this purpose, the potential of second and third harmonic generation (SHG, THG) microscopy is explored. SHG and THG are nonlinear optical microscopic techniques that do not require labeling of tissue to generate 3D images with intrinsic depth-sectioning at sub-cellular resolution. Until now, this technique had been applied on fixated breast tissue or to visualize the stroma only, whereas most tumors start in the lobules and ducts...
January 26, 2019: Journal of Biophotonics
Fahad Aljekhedab, Wenbin Zhang, Harold K Haugen, Gregory R Wohl, Munir M El-Desouki, Qiyin Fang
Ultrafast lasers are promising tools for surgical applications requiring precise tissue cutting. Shallow ablation depth and slow rate as well as collateral damage are common barriers limiting the use of laser in clinical applications. Localized cooling with water and/or air jet is known to reduce collateral thermal damage. We studied the influence of environmental conditions including air, compressed air flow, still water and water jet on ablation depth, ablation rate, and surface morphology on bovine bone samples with an 800 nm femtosecond laser...
January 24, 2019: Journal of Biophotonics
Weijun Xuan, Liyi Huang, Yuguang Wang, Xiaoqing Hu, Grzegorz Szewczyk, Ying-Ying Huang, Ahmed El-Hussein, Jerry C Bommer, Mark L Nelson, Tadeusz Sarna, Michael R Hamblin
Antimicrobial photodynamic inactivation (aPDI) employs the combination of non-toxic photosensitizing dyes and visible light to kill pathogenic microorganisms regardless of drug-resistance, and can be used to treat localized infections. A meso-substituted tetra-methylpyridinium porphyrin with one methyl group replaced by a C12 alkyl chain (FS111) and its Pd-derivative (FS111-Pd) were synthesized and tested as broad-spectrum antimicrobial photosensitizers when excited by blue light (5 or 10 J/cm2 ). Both compounds showed unprecedented activity, with the superior FS111-Pd giving 3 logs of killing at 1 nM, and eradication at 10 nM for Gram-positive methicillin-resistant Staphylococcus aureus...
January 22, 2019: Journal of Biophotonics
Yashveer Singh, Aniket Chowdhury, Chandrachur Mukherjee, Raktim Dasgupta, Shovan Kumar Majumder
Simultaneous photoreduction and Raman spectroscopy with 532 nm laser has been used to study the effects of organophosphate (chlorpyrifos) exposure on human red blood cells. Since in red blood cells, auto-oxidation causes oxidative stress, which, in turn, is balanced by the cellular detoxicants, any possible negative effect of chlorpyrifos on this balance should results in an increased level of damaged (permanently oxygenated) hemoglobin. Therefore, when 532 nm laser, at a suitable power, was applied to photoreduce the cells, only common oxygenated form of hemoglobin got photoreduced leaving the permanently oxygenated hemoglobin detectable in the Raman spectra simultaneously excited by the same laser...
January 21, 2019: Journal of Biophotonics
Menghan Wang, Nanguang Chen
Fluorescence imaging in the second near-infrared optical window (NIR-II, 900-1700 nm) has become a technique of choice for non-invasive in vivo imaging in recent years. Greater penetration depths with high spatial resolution and low background can be achieved with this NIR-II window, owing to low autofluorescence within this optical range and reduced scattering of long wavelength photons. Here, we present a novel design of confocal laser scanning microscope tailored for imaging in the NIR-II window. We showcase the outstanding penetration depth of our confocal setup with a series of imaging experiments...
January 21, 2019: Journal of Biophotonics
C Ruini, D Hartmann, M Bastian, T Ruzicka, L French, C Berking, T V Braunmühl
Actinic keratosis (AKs) corresponds to the earliest stage of in-situ squamous cell carcinoma and arises on chronically sun-exposed skin. Around the clinically evident AKs, the apparently healthy epidermis may contain different grades of atypia that can be detected by non-invasive imaging techniques such as reflectance confocal microscopy (RCM) and optical coherence tomography (OCT). Subclinical actinic keratosis (sAKs) have captured increasing interest as a potential target of field therapies. The aim of this study was to evaluate in vivo the changes in the field cancerization undergoing treatment with topical ingenolmebutate by combining RCM and OCT...
January 17, 2019: Journal of Biophotonics
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