Development of a multi-residue high-throughput UHPLC-MS/MS method for routine monitoring of SARM compounds in equine and bovine blood

Emiliano Ventura, Anna Gadaj, Tom Buckley, Mark H Mooney
Drug Testing and Analysis 2020 June 10
Selective androgen receptor modulators (SARMs) are a group of anabolic enhancer drugs posing threats to the integrity of animal sports and the safety of animal-derived foods. The current research describes for the first time the development of a semi-quantitative assay for SARM family compound monitoring in blood and establishes the relative stability of these analytes under various storage conditions prior to analysis. The presented screening method validation was performed in line with current EU legislation for the inspection of livestock and produce of animal origin, with CCβ values determined at 0.5 ng mL-1 (Ly2452473), 1 ng mL-1 (AC-262536 and PF-06260414), 2 ng mL-1 (bicalutamide, GLPG0492, LGD-2226, ostarine, S-1, S-6, and S-23) and 5 ng mL-1 (andarine, BMS-564929, LGD-4033, RAD140, and S-9), respectively. Applicability of the developed assay was demonstrated through the analysis of blood samples from racehorses and cattle. The developed method presents a high-throughput cost-effective tool for routine screening for a range of SARM compounds in sport and livestock animals.

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