Intravenous administration of multipotent stromal cells and bone allograft modification to enhance allograft healing.

AIM: This study investigated a coordinated strategy of revitalizing bone allograft with circulating multipotent stromal cells (MSCs).

MATERIALS & METHODS: After chemotactic and releasing assessments, stromal cell-derived factor 1 and platelet-derived growth factor BB in copolymers were coated on the bone allograft (AlloS-P ). Allograft coated with copolymers alone (Allo), as controls, or AlloS-P was implanted into the femur of athymic mice, which received intravenous injections of human MSCs or saline at weeks 1, 2 and 3.

RESULTS: At week 8, the total callus volume (both cartilaginous and bony callus) around the allograft was the largest in the AlloS-P  + MSC group (p < 0.05).

CONCLUSION: Coating bone allograft with stromal cell-derived factor 1 and platelet-derived growth factor BB and intravenous injections of MSCs improved allograft incorporation.