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Regenerative Medicine

Amit K Srivastava, Karthik S Prabhakara, Daniel J Kota, Supinder S Bedi, Fabio Triolo, Katherine S Brown, Matthew L Skiles, Heather L Brown, Charles S Cox, Scott D Olson
Aim: Traumatic brain injury is a complex condition consisting of a mechanical injury with neurovascular disruption and inflammation with limited clinical interventions available. A growing number of studies report systemic delivery of human umbilical cord blood (HUCB) as a therapy for neural injuries. Materials & methods: HUCB cells from five donors were tested to improve blood-brain barrier integrity in a traumatic brain injury rat model at a dose of 2.5 × 107 cells/kg at 24 or 72 h postinjury and for immunomodulatory activity in vitro ...
May 10, 2019: Regenerative Medicine
Ben Antebi, Kerfoot P Walker, Arezoo Mohammadipoor, Luis A Rodriguez, Robbie K Moore, Leopoldo C Cancio, Andriy I Batchinsky
Aim: In this study, we aimed at identifying the optimal conditions for isolation, processing and expansion of mesenchymal stem cells (MSCs). Methods: Porcine bone marrow was obtained from either small- or large-volume bone marrow aspirate (BMA). Next, three BMA processing methods were compared. Finally, the best condition was selected from various culture parameters, including basal media, supplementation and seeding density. Results: Our results demonstrate that a small-volume BMA and direct plating yields significantly higher concentration of MSCs...
May 9, 2019: Regenerative Medicine
Cleo D Stafford, Ricardo E Colberg, Alexander L Nourse
We present a case of an 18-year-old with a 2-year history of buttock pain who failed extensive treatment for a perceived hamstring strain. Upon evaluation, he was diagnosed with an ischial tuberosity nonunion avulsion fracture. The patient underwent bone grafting via an ultrasound guided leukocyte rich platelet-rich plasma injection followed by the use of a bone stimulator to enhance the bone healing. At 3 months, he was asymptomatic and had radiographic evidence of excellent bone healing. He remained asymptomatic at 1 year and had resumed full activities...
May 9, 2019: Regenerative Medicine
Ian A White, Alfonso L Sabater
No abstract text is available yet for this article.
May 9, 2019: Regenerative Medicine
Cailing Tong, Junjie Xia, Baiyi Xie, Minghui Li, Feifei Du, Cheng Li, Yaguang Li, Zhonggui Shan, Zhongquan Qi
Aim: Cardiac extracellular matrix (cECM) scaffolds are promising biomaterials for clinical applications. Our aim is to determine the immunogenicity of decellularized scaffolds from different sources for use as artificial organs during organ transplantation. Materials & methods: We transplanted Lewis rats with syngeneic (Lewis rat cECM), allogeneic (BN rat cECM) or xenogeneic (hamster cECM) decellularized cardiac scaffolds. Acute vascular and cellular rejection was quantified by immunohistochemistry and immune cell infiltration...
May 9, 2019: Regenerative Medicine
Pedro S Couto, Gocha Shatirishvili, Alexey Bersenev, Frances Verter
Aim: This is the first analysis of both clinical trials and published studies that employ umbilical cord mesenchymal stromal cells, for the decade 2007-2017. Materials & methods: Searching international databases, we found 178 registered trials and 98 publications. Results: Among the registered clinical trials, 20% have resulted in publications so far. Among the publications, 18% report safety and 74% report some form of improvement. Between 36 and 45% of the publications do not report aspects of the cell manufacturing, including isolation method, culture medium or number of culture passages...
May 9, 2019: Regenerative Medicine
Dusko Ilic
Latest developments in the field of stem cell research and regenerative medicine compiled from publicly available information and press releases from nonacademic institutions in March 2019.
May 3, 2019: Regenerative Medicine
Jonathan Stem, John C Flickinger, Dante Merlino, Ellen M Caparosa, Adam E Snook, Scott A Waldman
Gastrointestinal cancers remain a tremendous burden on society. Despite advances in therapy options, including chemotherapy and radiation, cancer mortality from recurrences and metastases occur frequently. Cancer stem cells (CSCs) drive disease recurrence and metastasis, as these cells are uniquely equipped to self-renew and evade therapy. Therefore, cancer eradication requires treatment strategies that target CSCs in addition to differentiated cancer cells. This review highlights current literature on therapies targeting CSCs in gastrointestinal cancer...
April 26, 2019: Regenerative Medicine
John T Paige, Michael Kremer, Jace Landry, Samuel A Hatfield, Donald Wathieu, Aaron Brug, Daniel J Lightell, Kara L Spiller, T Cooper Woods
Aim: To determine if porcine urinary bladder matrix (UBM) treatment is associated with modulation of wound inflammation in diabetic patients. Patients & methods: mRNA associated with M1 and M2 macrophages were measured in wounds of diabetic and nondiabetic patients pre- and post-treatment with UBM and an M1:M2 score was calculated. Results: Wound tissue from diabetic subjects exhibited elevated M1:M2 scores compared with nondiabetic patients, suggesting a greater pro-inflammatory state prior to treatment...
April 25, 2019: Regenerative Medicine
Dusko Ilic
Latest developments in the field of stem cell research and regenerative medicine compiled from publicly available information and press releases from nonacademic institutions in February 2019.
April 16, 2019: Regenerative Medicine
Agnieszka Borkowska-Kuczkowska, Dominika Sługocka, Beata Świątkowska-Flis, Dariusz Boruczkowski
Some ocular diseases, such as dystrophies, retinal and macular degeneration, optic nerve atrophy, and Stargardt disease, are progressive and irreversible. In this review, we focus on the use of mesenchymal stem cells (MSCs) in the treatment of these diseases. In animal studies, MSC transplantation significantly delayed retinal degeneration, led to the regeneration of cone cells, and supported the survival of retinal ganglion cells and axon regeneration. In clinical practice, patients with Behcet's disease with retinal vasculitis who received MSC injections experienced a decrease in retinal vasculitis but no improvement in vision acuity...
April 12, 2019: Regenerative Medicine
Dusko Ilic
Latest developments in the field of stem cell research and regenerative medicine compiled from publicly available information and press releases from nonacademic institutions in January 2019.
April 2, 2019: Regenerative Medicine
Oren Ben-Yosef, Jorge S Burns, Justin A Colacino, Daniel J Kota, Bruno Péault
No abstract text is available yet for this article.
March 19, 2019: Regenerative Medicine
Joan Oliva, Arjie Florentino, Fawzia Bardag-Gorce, Yutaka Niihara
AIM: The study goals were to engineer and harvest scaffold-free undifferentiated/differentiated multilayer human adipose-derived stem cell (hADSC) cell sheets, in absence of treatment. MATERIALS & METHODS:  The hADSC are seeded in 35 mm culture dishes. At confluence or when multilayer cell sheets are formed, hADSC are treated with predefined differentiation culture media (adipocyte, chondrocyte and osteoblast). RESULTS: Undifferentiated hADSC and differentiated adipocyte, osteoblast and chondrocyte hADSC multilayer cell sheets (hADSCmCS) have been harvested...
March 4, 2019: Regenerative Medicine
Li Chen, Zheng-Cai Wang, Jing-Jing Ma, Wen-Jia Sun, Shao-Wen Wang, Zi-Chun Gu, Xuan Yang
AIM: This study explored the effects of local transplantation of autologous nanofat in the treatment of rats with diabetic foot wounds. MATERIALS & METHODS: Nanofat was transplanted into the left foot wound of diabetic rats. Phosphate-buffered saline injection in the right served as control. We measured wound size, the extent of epithelization, microvessel density and the expression levels of cytokines at six different time-points postoperation. RESULTS: Compared with the control feet, nanofat-treated feet had significantly smaller wound areas at 7 and 9 days after grafting and showed better re-epithelialization, a greater number of microvessels and higher levels of angiogenic factor expression...
February 27, 2019: Regenerative Medicine
Serkan Dikici, Naşide Mangır, Frederik Claeyssens, Muhammad Yar, Sheila MacNeil
AIM: In this study, we explored the angiogenic potential and proangiogenic concentration ranges of 2-deoxy-D-ribose (2dDR) and 17β-Estradiol (E2) in comparison with VEGF. The 2dDR and E2 were then loaded into tissue engineering (TE) scaffolds to investigate their proangiogenic potential when released from fibers. MATERIALS & METHODS: Ex ovo chick chorioallantoic membrane (CAM) assay was used to evaluate angiogenic activity of 2dDR and E2. Both factors were then introduced into scaffolds via electrospinning to assess their angiogenic potential when released from fibers...
February 22, 2019: Regenerative Medicine
Shimeng Ji, Chaomin Wu, Lin Tong, Linlin Wang, Jian Zhou, Cuicui Chen, Yuanlin Song
Aim: To determine the efficiency of mesenchymal stem cells (MSCs) of different sources on airway epithelial cells regeneration and track where and to what extent transplanted MSCs home to injured tissues. Materials & methods: We performed DiO-labeled human bone marrow-derived MSCs (hBMSCs) or human chorionic villi-derived MSCs transplantation studies using naphthalene-induced airway injury animal models. Results: Compared with human chorionic villi-derived MSCs, hBMSCs facilitated airway epithelium regeneration faster and better from day 5 after transplantation; moreover, more transplanted hBMSCs distributed in injured lung tissues at the early stage of postinjury, which was mediated by C-X-C motif chemokine ligand 12...
March 2019: Regenerative Medicine
Glyn N Stacey, Peter W Andrews, Ivana Barbaric, Charlotta Boiers, Amit Chandra, Giulio Cossu, Lynn Csontos, Thomas Jr Frith, Jason A Halliwell, Zoe Hewitt, Mark McCall, Harry D Moore, Malin Parmar, M Beatrice Panico, Venkat Pisupati, Valentin P Shichkin, Alison R Stacey, Francesco S Tedesco, Oliver Thompson, Ravenska Wagey
Human stem cells have the potential to transform medicine. However, hurdles remain to ensure that manufacturing processes produce safe and effective products. A thorough understanding of the biological processes occurring during manufacture is fundamental to assuring these qualities and thus, their acceptability to regulators and clinicians. Leaders in both human pluripotent and somatic stem cells, were brought together with experts in clinical translation, biomanufacturing and regulation, to discuss key issues in assuring appropriate manufacturing conditions for delivery of effective and safe products from these cell types...
March 2019: Regenerative Medicine
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