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Regenerative Medicine

Elham Shojafar, Malek Soleimani Mehranjani, Seyed Ma Shariatzadeh
Aim: The effect of platelet-rich fibrin (PRF) bioscaffold on the structure and function of mice-autotransplanted ovaries was investigated. Materials & methods: Mice were divided into three groups: control, autografted and autografted + PRF bioscaffold. Angiogenesis, ovary histology and serum biochemical factors were assessed. Results: The total volume of the ovary, the number of follicles and the level of superoxide dismutase activity, total antioxidant capacity, IL-10, progesterone and estradiol were significantly higher in the autografted + PRF bioscaffold group compared with the autografted group...
June 12, 2019: Regenerative Medicine
Matej Buzgo, Martin Plencner, Michala Rampichova, Andrej Litvinec, Eva Prosecka, Andrea Staffa, Martin Kralovic, Eva Filova, Miroslav Doupnik, Vera Lukasova, Karolina Vocetkova, Jana Anderova, Tereza Kubikova, Robert Zajicek, Frantisek Lopot, Karel Jelen, Zdenek Tonar, Evzen Amler, Radek Divin, Fabrizio Fiori
Aim: This study evaluates the effect of electrospun dressings in critical sized full-thickness skin defects in rabbits. Materials & methods: Electrospun poly-ε-caprolactone (PCL) and polyvinyl alcohol (PVA) nanofibers were tested in vitro and in vivo . Results: The PCL scaffold supported the proliferation of mesenchymal stem cells, fibroblasts and keratinocytes. The PVA scaffold showed significant swelling, high elongation capacity, limited protein adsorption and stimulation of cells. Nanofibrous dressings improved wound healing compared with the control group in vivo ...
June 10, 2019: Regenerative Medicine
James W Reinhardt, Juan de Dios Ruiz Rosado, Jenny C Barker, Yong-Ung Lee, Cameron A Best, Tai Yi, Qiang Zeng, Santiago Partida-Sanchez, Toshiharu Shinoka, Christopher K Breuer
Aim: To characterize early events in neotissue formation during the first 2 weeks after vascular scaffold implantation. Materials & methods: Biodegradable polymeric scaffolds were implanted as abdominal inferior vena cava interposition grafts in wild-type mice. Results: All scaffolds explanted at day 1 contained a platelet-rich mural thrombus. Within the first few days, the majority of cell infiltration appeared to be from myeloid cells at the peritoneal surface with modest infiltration along the lumen...
June 10, 2019: Regenerative Medicine
Dusko Ilic
Latest developments in the field of stem cell research and regenerative medicine compiled from publicly available information and press releases from nonacademic institutions in April 2019.
June 7, 2019: Regenerative Medicine
Wenxiang Chu, Xin Wang, Yaokai Gan, Yifu Zhuang, Dingwei Shi, Fengxiang Liu, Yuehua Sun, Jie Zhao, Tingting Tang, Kerong Dai
Aim: To evaluate the clinical efficacy of mesenchymal stem cell/β-tricalcium phosphate composites (MSC/β-TCP) prepared with a screen-enrich-combine circulating system (SECCS) in patients with depressed tibial plateau fractures. Materials & methods: Bone defects in depressed tibial plateaus were filled with MSC/β-TCP (n = 16) or with β-TCP only (n = 23). Enrichment efficiency and effect of enrichment on cell viability were evaluated. Clinical results were assessed by imaging examination and Lysholm score...
May 22, 2019: Regenerative Medicine
Alison Wilson, Andrew Webster, Paul Genever
Mesenchymal stem cells (MSCs) are in development for many clinical indications, based both on 'stem' properties (tissue repair or regeneration) and on signaling repertoire (immunomodulatory and anti-inflammatory effects). Potential conflation of MSC properties with those of tissue-derived stromal cells presents difficulties in comparing study outcomes and represents a source of confusion in cell therapy development. Cultured MSCs demonstrate significant heterogeneity in clonogenicity and multi-lineage differentiation potential...
May 22, 2019: Regenerative Medicine
Anuradha Vaidya, Shweta Singh, Lalita Limaye, Vaijayanti Kale
Aim: To examine whether AKT-modified stromal cells expand human CD34+ hematopoietic stem cells (HSCs). Methods: Coculture, in vitro functional assays, immuno-fluorescence microscopy, flow cytometry. Results: M2-10B4 stromal cells (M2) modified with AKT1 (M2-AKT) expanded primitive CD34+ 38- HSCs, but affected their functionality. A chimeric feeder layer comprising naive human bone marrow-derived mesenchymal stromal cells and M2-AKT not only overcame the negative effects of M2-AKT, but, unexpectedly, also gave a synergistic effect on the growth and functionality of the HSCs...
May 22, 2019: Regenerative Medicine
Chad C MacArthur, Suman Pradhan, Nichole Wetton, Aryan Zarrabi, Carl Dargitz, Mahalakshmi Sridharan, Stephen Jackson, Loni Pickle, Uma Lakshmipathy
Induced pluripotent stem cells (iPSCs) hold immense potential in disease modeling, drug discovery and regenerative medicine. Despite advances in reprogramming methods, generation of clinical-grade iPSCs remains a challenge. Reported here is the first off-the-shelf reprogramming kit, CTS CytoTune-iPS 2.1, specifically designed for clinical and translational research. Workflow gaps were identified, and methods developed were used to consistently generate iPSC from multiple cell types. Resulting clones were subjected to characterization that included confirmation of pluripotency, preservation of genomic integrity and authentication of cell banks via an array of molecular methods including high resolution microarray and next-generation sequencing...
May 22, 2019: Regenerative Medicine
Stephany C Huber, José Luiz R C Junior, Letícia Q Silva, Silmara A L Montalvão, Joyce M Annichino-Bizzacchi
Aim: To compare freeze-dried and fresh platelet-rich plasma (PRP) preparations, in a pre-clinical study. Materials & methods:  30 Wistar male rats were used to compare and characterize human PRP which was applied at the perilesional area in an acute wound model, evaluated by macroscopical and histological analysis. Results: Despite the increased growth factor concentration after the freeze-drying process, no change in the healing kinetics was observed in vivo . Nevertheless, a significant increased number of myofibroblasts was demonstrated in comparison with the fresh PRP group...
May 22, 2019: Regenerative Medicine
Mark A Sussman
No abstract text is available yet for this article.
May 22, 2019: Regenerative Medicine
Cheng-Yoong Pang, Kuo-Liang Yang, Chin-Hua Fu, Li-Yi Sun, Shin-Yuan Chen, Chia-Hsin Liao
Aim: The therapeutic effects of human wisdom teeth-derived neuronal stem cell (tNSC) cotreatment with granulocyte-colony-stimulating factor (G-CSF) were evaluated for contusion-induced spinal cord injury in rats. Materials & methods:  7 days after contusion, tNSCs were transplanted to the injury site and followed by G-CSF cotreatment for 5 days. Behavioral deficits were evaluated by the Basso, Beattie and Bresnahan test. The injury site was collected for immunohistochemistry analysis. Results: The Basso, Beattie and Bresnahan test significantly improved in the cotreated group compared with the tNSCs or G-CSF single treatment groups...
May 22, 2019: Regenerative Medicine
Mohsin A Hussain, Martina Colicchia, Jessry Veerapen, Deshan Weeraman, Mihai-Nicolae Podaru, Daniel Jones, Ken Suzuki, Anthony Mathur
Stem cell therapy utilizing bone marrow mononuclear cells (BMC's) is a potential strategy to treat heart failure patients with improvement in symptom profile and cardiac function. We describe a rationale for concurrent BMC and left ventricular assist device therapy in selected heart failure patients. This combination therapy has demonstrated improved myocardial perfusion and cardiac function in patients with advanced ischemic cardiomyopathy. Moreover, preclinical data support improved cell retention with left ventricular unloading...
May 22, 2019: Regenerative Medicine
Amit K Srivastava, Karthik S Prabhakara, Daniel J Kota, Supinder S Bedi, Fabio Triolo, Katherine S Brown, Matthew L Skiles, Heather L Brown, Charles S Cox, Scott D Olson
Aim: Traumatic brain injury is a complex condition consisting of a mechanical injury with neurovascular disruption and inflammation with limited clinical interventions available. A growing number of studies report systemic delivery of human umbilical cord blood (HUCB) as a therapy for neural injuries. Materials & methods: HUCB cells from five donors were tested to improve blood-brain barrier integrity in a traumatic brain injury rat model at a dose of 2.5 × 107 cells/kg at 24 or 72 h postinjury and for immunomodulatory activity in vitro ...
May 10, 2019: Regenerative Medicine
Ben Antebi, Kerfoot P Walker, Arezoo Mohammadipoor, Luis A Rodriguez, Robbie K Moore, Leopoldo C Cancio, Andriy I Batchinsky
Aim: In this study, we aimed at identifying the optimal conditions for isolation, processing and expansion of mesenchymal stem cells (MSCs). Methods: Porcine bone marrow was obtained from either small- or large-volume bone marrow aspirate (BMA). Next, three BMA processing methods were compared. Finally, the best condition was selected from various culture parameters, including basal media, supplementation and seeding density. Results: Our results demonstrate that a small-volume BMA and direct plating yields significantly higher concentration of MSCs...
May 9, 2019: Regenerative Medicine
Cleo D Stafford, Ricardo E Colberg, Alexander L Nourse
We present a case of an 18-year-old with a 2-year history of buttock pain who failed extensive treatment for a perceived hamstring strain. Upon evaluation, he was diagnosed with an ischial tuberosity nonunion avulsion fracture. The patient underwent bone grafting via an ultrasound guided leukocyte rich platelet-rich plasma injection followed by the use of a bone stimulator to enhance the bone healing. At 3 months, he was asymptomatic and had radiographic evidence of excellent bone healing. He remained asymptomatic at 1 year and had resumed full activities...
May 9, 2019: Regenerative Medicine
Ian A White, Alfonso L Sabater
No abstract text is available yet for this article.
May 9, 2019: Regenerative Medicine
Cailing Tong, Junjie Xia, Baiyi Xie, Minghui Li, Feifei Du, Cheng Li, Yaguang Li, Zhonggui Shan, Zhongquan Qi
Aim: Cardiac extracellular matrix (cECM) scaffolds are promising biomaterials for clinical applications. Our aim is to determine the immunogenicity of decellularized scaffolds from different sources for use as artificial organs during organ transplantation. Materials & methods: We transplanted Lewis rats with syngeneic (Lewis rat cECM), allogeneic (BN rat cECM) or xenogeneic (hamster cECM) decellularized cardiac scaffolds. Acute vascular and cellular rejection was quantified by immunohistochemistry and immune cell infiltration...
May 9, 2019: Regenerative Medicine
Pedro S Couto, Gocha Shatirishvili, Alexey Bersenev, Frances Verter
Aim: This is the first analysis of both clinical trials and published studies that employ umbilical cord mesenchymal stromal cells, for the decade 2007-2017. Materials & methods: Searching international databases, we found 178 registered trials and 98 publications. Results: Among the registered clinical trials, 20% have resulted in publications so far. Among the publications, 18% report safety and 74% report some form of improvement. Between 36 and 45% of the publications do not report aspects of the cell manufacturing, including isolation method, culture medium or number of culture passages...
May 9, 2019: Regenerative Medicine
Dusko Ilic
Latest developments in the field of stem cell research and regenerative medicine compiled from publicly available information and press releases from nonacademic institutions in March 2019.
May 3, 2019: Regenerative Medicine
Jonathan Stem, John C Flickinger, Dante Merlino, Ellen M Caparosa, Adam E Snook, Scott A Waldman
Gastrointestinal cancers remain a tremendous burden on society. Despite advances in therapy options, including chemotherapy and radiation, cancer mortality from recurrences and metastases occur frequently. Cancer stem cells (CSCs) drive disease recurrence and metastasis, as these cells are uniquely equipped to self-renew and evade therapy. Therefore, cancer eradication requires treatment strategies that target CSCs in addition to differentiated cancer cells. This review highlights current literature on therapies targeting CSCs in gastrointestinal cancer...
April 26, 2019: Regenerative Medicine
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