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Regenerative Medicine

Akira Akabayashi
No abstract text is available yet for this article.
August 23, 2019: Regenerative Medicine
Dusko Ilic, Mirjana Liovic, Laila Noli
The latest developments in the field of stem cell research and regenerative medicine compiled from publicly available information and press releases from nonacademic institutions in June 2019.
August 23, 2019: Regenerative Medicine
Kayo Takashima, Yusuke Inoue, Shimon Tashiro, Kaori Muto
No abstract text is available yet for this article.
August 22, 2019: Regenerative Medicine
Zhanjun Ma, Yubao Lu, Yang Yang, Jing Wang, Xuewen Kang
Spinal cord injury (SCI) is one of the leading causes of global disability. However, there are currently no effective clinical treatments for SCI. Repair of SCI is essential but poses great challenges. As a comprehensive treatment program combining biological scaffolds, seed cells and drugs or biological factors, tissue engineering has gradually replaced the single transplantation approach to become a focus of research that brings new opportunities for the clinical treatment of SCI.
August 22, 2019: Regenerative Medicine
Nicholas R Beatty, Cole Lutz, Kwadwo Boachie-Adjei, Teresita A Leynes, Christopher Lutz, Gregory Lutz
A 40-year-old woman with a history of chronic low back pain underwent a fluoroscopically guided intradiscal platelet-rich plasma injection (PRP) at the L5-S1 level. She subsequently developed progressive low back pain, night sweats and decreased ability to ambulate. Laboratory work-up revealed elevated acute phase reactants and imaging revealed L5-S1 intervertebral disc and vertebral end-plate abnormalities highly suggestive of spondylodiscitis. Computed tomography-guided aspiration and biopsy cultures grew Cutibacterium acnes and the patient was subsequently treated with intravenous antibiotics without surgical management...
August 19, 2019: Regenerative Medicine
Aimaiti Yasen, Tuerhongjiang Tuxun, Shadike Apaer, Wending Li, Yusufukadier Maimaitinijiati, Hui Wang, Meiheriayi Aisan, Tuerganaili Aji, Yingmei Shao, Wen Hao
Stem cell transplantation exhibited a promising lifesaving therapy for various end-stage liver diseases and could serve as a salvaging bridge until curative methods can be performed. In past decades, mature hepatocytes, liver progenitor cells, mesenchymal stem cells and induced pluripotent stem cells have been practiced in above settings. However, long-term survival rates and continuous proliferation ability of these cells in vivo are unsatisfactory, whereas, fetal liver stem cells (FLSCs), given their unique superiority, may be the best candidate for stem cell transplantation technique...
August 8, 2019: Regenerative Medicine
Charles C Lee, Naoki Hirasawa, Katrina G Garcia, Dinesh Ramanathan, Kee D Kim
Stem cells reside in their native microenvironment, which provides dynamic physical and chemical cues essential to their survival, proliferation and function. A typical cell-based therapeutic approach requires the mesenchymal stem cells (MSC) to depart their native microenvironment, transplant to in-vivo environment, differentiate toward multiple lineages and participate in bone formation. The long-term survival, function and fate of MSC are dependent on the microenvironment in which they are transplanted. Transplantation of morselized autologous bone, which contains both stem cells and their native microenvironment, results in a good clinical outcome...
August 8, 2019: Regenerative Medicine
Xinyu Zhang, Shune Xiao, Bingcheng Liu, Yong Miao, Zhiqi Hu
Aim: To explore the feasibility of human placenta extracellular matrix (HPECM) hydrogel in restoring the hair-inductive capacity of high-passaged (P8) dermal papilla cells (DPCs) for hair follicle regeneration. Materials & methods: HPECM hydrogel was prepared following decellularization and enzymatic solubilization treatment. DPCs isolated from human scalp were cultured in 2D and 3D environments. The hair-inductive ability of DPCs was assessed by quantitative RT-PCR, immunofluorescence staining, immunoblotting and patch assay...
August 1, 2019: Regenerative Medicine
Sindhuja D Eswaramoorthy, Nandini Dhiman, Gayathri Korra, Carlo M Oranges, Dirk J Schaefer, Subha N Rath, Srinivas Madduri
Aim: We investigated the role of induced endothelial cells (iECs) in mesenchymal stem cells (MSCs)/iECs co-culture and assessed their osteogenic ability on silk fibroin nanofiber scaffolds. Methods: The osteogenic differentiation was assessed by the ALP assay, calcium assay and gene expression studies. Results: The osteogenic differentiation of the iECs co-cultures was found to be higher than the MSCs group and proximal to endothelial cells (ECs) co-cultures. Furthermore, the usage of isogenic iECs for co-culture increased the osteogenic and endothelial gene expression...
July 26, 2019: Regenerative Medicine
Riddhima Agarwal, Kevin M Blum, Andrew Musgrave, Ekene A Onwuka, Tai Yi, James W Reinhardt, Cameron A Best, Christopher K Breuer
Aim: This study evaluates scaffold degradation and neotissue formation as a function of sealant polymer composition in tissue-engineered vascular grafts (TEVGs). Materials & methods: Scaffolds fabricated from polyglycolic acid core and sealant composed of polycaprolactone (PCL), poly-L-lactic-acid (PLLA) or 50:50 copolymer poly(ε-caprolactone-co-L-lactide) (PCLA) were analyzed in vitro using accelerated degradation and scanning electron microscopy, and in vivo following implantation in a murine inferior vena cava interposition model...
July 25, 2019: Regenerative Medicine
Saranya P Wyles, Andre Terzic
No abstract text is available yet for this article.
July 25, 2019: Regenerative Medicine
Venkata P Mantripragada, Nicolas S Piuzzi, Jaiben George, Wesley Bova, Mitchell Ng, Cynthia Boehm, George F Muschler
Aim: A limiting factor in advancement of bone marrow based cell therapies is the lack of characterization of cell products delivered to patients. Methods: Using an automated hematology analyzer that can be implemented in clinical setting, the composition of bone marrow aspirates (n = 17 patients) and bone marrow concentrates (n = 12 patients) were assessed. ICC estimates were calculated for measuring reliability. Results: Bone marrow aspirates assessment resulted in excellent reliability for determining white blood cells (ICC - 0...
July 19, 2019: Regenerative Medicine
Mukta S Sane, Huiyuan Tang, Neha Misra, Xinzhu Pu, Sara Malara, Christopher D Jones, Soumyajit Banerjee Mustafi
Aim: Umbilical cord blood (UCB) sourced allografts are promising interventions for tissue regeneration. As applications of these allografts and regulations governing them continue to evolve, we were prompted to identify parameters determining their quality, safety and regenerative potential. Materials & methods: Flow-cytometry, mass-spectrometry, protein multiplexing, nanoparticle tracking analysis and standard biological techniques were employed. Results: Quality attributes of a uniquely processed UCB-allograft (UCBr) were enumerated based on identity (cell viability, immunophenotyping, proteomic profiling, and quantification of relevant cytokines); safety (bioburden and microbiological screening), purity (endotoxin levels) and potency (effect of UCBr on chondrocytes and mesenchymal stem cells derived exosomes)...
July 17, 2019: Regenerative Medicine
Chenjun Zhai, Xiao Zhang, Jun Chen, Jian He, Hao Fei, Yang Liu, Chunyang Luo, Weimin Fan
Aim: To investigate the effect of cartilage extracellular matrix (ECM) particle size on the chondrogenic differentiation of bone marrow-derived mesenchymal stem cells (BMSCs). Materials & methods: BMSCs were seeded into the scaffolds fabricated by small particle ECM materials and large particle ECM materials. For the positive control, chondrogenically induced BMSCs were seeded into commercial poly-lactic-glycolic acid scaffolds. Macroscopic observation, histological and immunohistochemical staining, mechanical testing and biochemical analysis were performed to the cell-scaffold constructs...
July 17, 2019: Regenerative Medicine
Robert A Kloner, Sharon L Hale, Wangde Dai
No abstract text is available yet for this article.
July 17, 2019: Regenerative Medicine
Matthias W Laschke, Alexander Heß, Claudia Scheuer, Philipp Karschnia, Elena Kontaxi, Michael D Menger
Aim: Adipose tissue-derived microvascular fragments (ad-MVF) are vascularization units for regenerative medicine. We investigated whether University of Wisconsin (UW) solution is suitable for their xeno-free storage. Materials & methods: Murine ad-MVF were cultivated for 24 h in 4°C or 20°C UW solution and 20°C endothelial cell growth medium (control). The ad-MVF were seeded onto collagen-glycosaminoglycan scaffolds, which were analyzed in dorsal skinfold chambers by intravital fluorescence microscopy and histology...
July 17, 2019: Regenerative Medicine
Dusko Ilic, Mirjana Liovic
Latest developments in the field of stem cell research and regenerative medicine compiled from publicly available information and press releases from nonacademic institutions in May 2019.
July 4, 2019: Regenerative Medicine
Sujith Sebastian, Paul Hourd, Amit Chandra, David J Williams, Nicholas Medcalf
Cell-based therapies must achieve clinical efficacy and safety with reproducible and cost-effective manufacturing. This study addresses process development issues using the exemplar of a human pluripotent stem cell-based dopaminergic neuron cell therapy product. Early identification and correction of risks to product safety and the manufacturing process reduces the expensive and time-consuming bridging studies later in development. A New Product Introduction map was used to determine the developmental requirements specific to the product...
June 18, 2019: Regenerative Medicine
Shang-Wen Wong, Shan-Ju Yeh, Cheng-Wei Li, Lily Hui-Ching Wang, Bor-Sen Chen
Aim: A systematic multimolecule drug design procedure is proposed for promoting hepatogenesis and liver regeneration. Materials & methods: Genome-wide microarray data including three hepatic conditions are obtained from the GEO database (GSE15238). System modeling and big data mining methods are used to construct real genome-wide genetic-and-epigenetic networks (GWGENs). Then, we extracted the core GWGENs by applying principal network projection on real GWGENs of normal, developing and regenerating livers, respectively...
June 17, 2019: Regenerative Medicine
Elham Shojafar, Malek Soleimani Mehranjani, Seyed Ma Shariatzadeh
Aim: The effect of platelet-rich fibrin (PRF) bioscaffold on the structure and function of mice-autotransplanted ovaries was investigated. Materials & methods: Mice were divided into three groups: control, autografted and autografted + PRF bioscaffold. Angiogenesis, ovary histology and serum biochemical factors were assessed. Results: The total volume of the ovary, the number of follicles and the level of superoxide dismutase activity, total antioxidant capacity, IL-10, progesterone and estradiol were significantly higher in the autografted + PRF bioscaffold group compared with the autografted group...
June 12, 2019: Regenerative Medicine
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