Transforming Growth Factor-β1 Stimulates Mesenchymal Stem Cells Proliferation via Altering Cell Cycle through FAK-Akt-mTOR Pathway.

BACKGROUND: Mesenchymal stem cells (MSCs) are promising for cell therapy and regenerative medicine. An increased need for expanding of MSCs under serum-free condition to achieve a sufficient quantity for therapeutic applications is inevitable. Transforming growth factor-β1 (TGF-β1) is widely used for expanding clinical-grade MSCs in vitro. This work focuses on the influence of TGF-β1 on proliferation in rat bone-marrow derived MSCs (BMSCs) and the underlying mechanism.

MATERIALS AND METHODS: BMSCs were isolated and cultured with or without TGF-β1 in a serum-free medium and Cell Counting Kit-8 assay was used to detect BMSCs proliferation. Cell cycle transition was also analyzed. Further, the expression levels of cyclin D1, phosphorylated focal adhesion kinase and downstream effectors in Akt-mTOR-S6K1 signaling pathway were examined by western blotting.

RESULTS AND CONCLUSION: TGF-β1 triggered proliferation via accelerating G1/S cell cycle transition in BMSCs. The addition of TGF-β1 can activate Akt-mTOR-S6K1 pathway. Additionally, FAK was found to be involved in the process. Upon adding the FAK inhibitor, the activation of Akt-mTOR-S6K1 and TGF-β1-induced cell proliferation were both abrogated. Together, an insight understanding of how TGF-β1 influences BMSCs proliferation is achieved. This study provides a possible strategy of supplementing TGF-β1 in serum-free medium for in vitro expansion, which eventually would advance the production of clinical-grade MSCs for regenerative medicine.