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Connective Tissue Research

Ra Jeong Kim, Young-Sool Hah, Ji-Yong Gwark, Hyung Bin Park
PURPOSE: Neuronal theory regarding rotator cuff degeneration has developed from the findings that glutamate, an amino acid and an excitatory neurotransmitter, is present in increased concentrations in tendon tissues with tendinopathy and that glutamate induces cell death in fibroblasts of origin in rat supraspinatus tendon. The purpose of the current study was to determine whether N-acetylcysteine (NAC) has cytoprotective effects against glutamate-induced fibroblast death. MATERIALS AND METHODS: Primary cultured fibroblasts were obtained from rat supraspinatus tendons...
February 12, 2019: Connective Tissue Research
Han-Wool Choung, Dong-Seol Lee, Yeoung-Hyun Park, Yoon Seon Lee, Shengfeng Bai, Su-Hyang Yoo, Jong-Ho Lee, Hyung-Keun You, Joo-Cheol Park
INTRODUCTION: Preameloblast-conditioned medium (PA-CM), as a mixture of dental epithelium-derived factors has been reported to regenerate dentin and periodontal tissues in vitro and in vivo. Recently, protein copine7 (Cpne7) which was found in PA-CM was suggested to have a beneficial role in periodontal tissue healing. The aim of this study was to investigate the biological effect of Cpne7 on the proliferation, migration, and cementoblast differentiation of periodontal cells in vitro, and on the regeneration of periodontal tissue using periodontal defect model with canine in vivo...
February 8, 2019: Connective Tissue Research
Joanna B Broad, Zhenqiang Wu, Taane G Clark, David Musson, Rebekah Jaung, Bruce Arroll, Ian P Bissett, Martin J Connolly
PURPOSE: An underlying connective tissue disorder (CTD) may predispose to formation of intestinal diverticula. We assess the association of diverticulosis with nine selected CTDs, to inform the pathophysiology of diverticula. METHODS: A population-based period-prevalence study. Individuals (3.5 million New Zealand residents born 1901-1986) with a health system record 1999-2016 were grouped into those with a hospital diagnosis of diverticulosis or diverticulitis (ICD-10-AM K57), and those without...
February 5, 2019: Connective Tissue Research
Marie Semaan, Elie Karam, Cécile Baron, Martine Pithioux
PURPOSE: Non-pathological child cortical bone (NPCCB) studies can provide clinicians with vital information and insights. However, assessing the anisotropic elastic properties of NPCCB remains a challenge for the biomechanical engineering community. For the first time, this paper provides elastic moduli values for NPCCB specimens in two perpendicular directions (longitudinal and transverse) and for two different structural components of bone tissue (osteon and interstitial lamellae). MATERIALS AND METHODS: Microindentation is one of the reference methods used to measure bone stiffness...
January 15, 2019: Connective Tissue Research
Jie Sun, Yan Zhou, Zhaoyang Ye, Wen-Song Tan
BACKGROUND: Mesenchymal stem cells (MSCs) are promising for cell therapy and regenerative medicine. An increased need for expanding of MSCs under serum-free condition to achieve a sufficient quantity for therapeutic applications is inevitable. Transforming growth factor-β1 (TGF-β1) is widely used for expanding clinical-grade MSCs in vitro. This work focuses on the influence of TGF-β1 on proliferation in rat bone-marrow derived MSCs (BMSCs) and the underlying mechanism. MATERIALS AND METHODS: BMSCs were isolated and cultured with or without TGF-β1 in a serum-free medium and Cell Counting Kit-8 assay was used to detect BMSCs proliferation...
January 15, 2019: Connective Tissue Research
Ming-Xi Liu, Qing-Yu Liu, Ye Liu, Zhi-Ming Cheng, Lei Liu, Lei Zhang, Da-Hui Sun
Purpose/Aim of the study: Interleukin (IL)-35 is a newly identified IL-12 cytokine family member and reveals immunosuppressive activity to CD8+ T cells in inflammation, infectious diseases, and cancers. However, little is known regarding IL-35 function in osteosarcoma. Thus, the aim of the current study was to investigate the regulatory function of IL-35 to CD8+ T cells in osteosarcoma. MATERIALS AND METHODS: Thirty-five osteosarcoma patients and 20 healthy individuals were enrolled. Serum CD4+ CD25+ CD127dim/ - regulatory T cells (Tregs) and CD8+ T cells were purified...
January 7, 2019: Connective Tissue Research
Megan K DeBari, Mia N Keyser, Michelle A Bai, Rosalyn D Abbott
Silk is a natural polymer sourced mainly from spiders and silkworms. Due to its biocompatibility, biodegradability, and mechanical properties, it has been heavily investigated for biomedical applications. It can be processed into a number of formats, such as scaffolds, films, and nanoparticles. Common methods of production create constructs with limited complexity. 3D printing allows silk to be printed into more intricate designs, increasing its potential applications. Extrusion and inkjet printing are the primary ways silk has been 3D printed, though other methods are beginning to be investigated...
December 17, 2018: Connective Tissue Research
Vivian H M Mouser, Riccardo Levato, Anneloes Mensinga, Wouter J A Dhert, Debby Gawlitta, Jos Malda
Bioprinting is a promising tool to fabricate organized cartilage. This study aimed to investigate the printability of gelatin-methacryloyl/gellan gum (gelMA/gellan) hydrogels with and without methacrylated hyaluronic acid (HAMA), and to explore (zone-specific) chondrogenesis of chondrocytes, articular cartilage progenitor cells (ACPCs), and multipotent mesenchymal stromal cells (MSCs) embedded in these bio-inks. The incorporating of HAMA in gelMA/gellan bio-ink increased filament stability, as measured using a filament collapse assay, but did not influence (zone-specific) chondrogenesis of any of the cell types...
December 10, 2018: Connective Tissue Research
Le Chang, Xin Feng, Wei Gao
Fibroblast-like synoviocytes (FLSs), with their tumor-like proliferation, play an important role in rheumatoid arthritis (RA), and interleukin-17 (IL-17) participates in RA pathology by affecting FLSs. The aims of this study were to investigate the effects of IL-17 on the proliferation and autophagy of FLSs and the role of signal transducer and activator of transcription-3 (STAT3) in RA. FLSs were treated with IL-17 at different concentrations (0, 1, 10, and 20 ng/mL); then, autophagy was assayed with western blotting, immunofluorescence and transmission electron microscopy...
November 26, 2018: Connective Tissue Research
Andrzej Fertala
No abstract text is available yet for this article.
January 2019: Connective Tissue Research
Coralee E Tye, Joseph R Boyd, Natalie A Page, Michelle M Falcone, Janet L Stein, Gary S Stein, Jane B Lian
Long noncoding RNAs (lncRNAs) have recently emerged as novel regulators of lineage commitment, differentiation, development, viability, and disease progression. Few studies have examined their role in osteogenesis; however, given their critical and wide-ranging roles in other tissues, lncRNAs are most likely vital regulators of osteogenesis. In this study, we extensively characterized lncRNA expression in mesenchymal cells during commitment and differentiation to the osteoblast lineage using a whole transcriptome sequencing approach (RNA-Seq)...
December 2018: Connective Tissue Research
Pascal R Buenzli, Chloé Lerebours, Andreas Roschger, Paul Roschger, Richard Weinkamer
PURPOSE: Experimental measurements of bone mineral density distributions (BMDDs) enable a determination of secondary mineralization kinetics in bone, but the maximum degree of mineralization and how this maximum is approached remain uncertain. We thus test computationally different hypotheses on late stages of bone mineralization by simulating BMDDs in low-turnover conditions. MATERIALS AND METHODS: An established computational model of the BMDD that accounts for mineralization and remodeling processes was extended to limit mineralization to various maximum calcium capacities of bone...
December 2018: Connective Tissue Research
Ceren Tuzmen, Phil G Campbell
AIM OF THE STUDY: The peripheral nervous system is involved in regulation of bone metabolism via sensory and sympathetic innervation. Substance P (SP) and calcitonin gene-related peptide (CGRP) are two sensory neuropeptides that have been associated with regulation of osteogenic differentiation. However, the interaction between SP and CGRP both with each other and the bone morphogenetic protein 2 (BMP2) in regulation of osteogenic differentiation has not been studied. Therefore, the aim of this study was to investigate the interaction between SP and CGRP on BMP2-induced bone differentiation using model progenitor cells...
December 2018: Connective Tissue Research
Scott V Wiener, Ling Chen, Alex R Shimotake, Misun Kang, Marshall L Stoller, Sunita P Ho
Purpose/Aim: The most common kidney stone composed of calcium oxalate forms on interstitial calcium phosphate mineral known as a Randall's plaque (RP). Due to limited information about events leading to the initial deposition of nanometer size interstitial calcium phosphate pre-clusters, there continues to be a debate on the initial site of calcium phosphate deposition and factors leading to stone formation. MATERIALS AND METHODS: High-resolution X-ray micro-computed tomography (CT), and light and electron microscopy techniques were used to characterize human renal pyramids and five representative kidney stones with identifiable stems...
December 2018: Connective Tissue Research
Benjamin R Coyac, Guillaume Falgayrac, Guillaume Penel, Alain Schmitt, Thorsten Schinke, Agnès Linglart, Marc D McKee, Catherine Chaussain, Claire Bardet
X-linked hypophosphatemia (XLH) is a skeletal disorder arising from mutations in the PHEX gene, transmitted in most cases as an X-linked dominant trait. PHEX deficiency leads to renal phosphate wasting and hypophosphatemia, as well as impaired mineralization of bone and dentin, resulting in severe skeletal and dental complications. Dentin mineralization defects appear as characteristic, large interglobular spaces resulting from the lack of fusion of calculospherites in the circumpulpal region during the mineralization process...
December 2018: Connective Tissue Research
Keith Alvares, Caroline J DeHart, Paul M Thomas, Neil L Kelleher, Arthur Veis
BACKGROUND: Matrix-regulated biomineralization involves the specific nucleation and growth of mineral phases within or upon preformed structured organic matrices. We hypothesized that there might be a general mechanism whereby anionic, phosphorylated mineral ion-binding proteins assist in specifically locating the mineral ions with respect to the mineralizing structural organic matrix. Here we extended these studies to invertebrate mineralization in Lytechinus variegatus (Lv) teeth. MATERIALS AND METHODS: The tooth proteins were extracted and the phosphoproteins occluded in the mineral were enriched by passage through a ProQ Diamond phosphoprotein enrichment column, and subjected to MS/MS analysis...
December 2018: Connective Tissue Research
Fleur Berès, Guilhem Lignon, Stéphan Rouzière, Cédric Mauprivez, Stéphane Simon, Ariane Berdal, Arnaud Dessombz
No abstract text is available yet for this article.
December 2018: Connective Tissue Research
Amsaveni Ramachandran, Kun He, Chun-Chieh Huang, Reza Shahbazian-Yassar, Tolou Shokuhfar, Anne George
In search for bone and dentin extracellular matrix (ECM) proteins, transforming growth factor beta receptor II interacting protein 1 (TRIP-1) was identified as a novel protein synthesized by osteoblasts and odontoblasts and exported to the ECM. TRIP-1 is a WD-40 (WD is Tryptophan-Aspartic acid dipeptide) protein that has been well recognized for its physiological role in the endoplasmic reticulum (ER). In the ER, TRIP-1 functions as an essential subunit of eukaryotic elongation initiation factor 3 and is involved in the protein translational machinery...
December 2018: Connective Tissue Research
Olivier Duverger, Maria I Morasso
DLX3 is essential for tooth enamel development and is so far the only transcription factor known to be mutated in a syndromic form of amelogenesis imperfecta. Through conditional deletion of Dlx3 in the dental epithelium in mouse, we have previously established the involvement of DLX3 in enamel pH regulation, as well as in controlling the expression of sets of keratins that contribute to enamel rod sheath formation. Here, we show that the decussation pattern of enamel rods was lost in conditional knockout animals, suggesting that DLX3 controls the coordinated migration of ameloblasts during enamel secretion...
December 2018: Connective Tissue Research
Yannicke Dauphin, Gilles Luquet, Alberto Perez-Huerta, Murielle Salomé
Avian eggshells are composed of several layers made of organic compounds and a mineral phase (calcite), and the general structure is basically the same in all species. A comparison of the structure, crystallography, and chemical composition shows that despite an overall similarity, each species has its own structure, crystallinity, and composition. Eggshells are a perfect example of the crystallographic versus biological concept of the formation and growth mechanisms of calcareous biominerals: the spherulitic-columnar structure is described as "a typical case of competitive crystal growth", but it is also said that the eggshell matrix components regulate eggshell mineralization...
December 2018: Connective Tissue Research
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