The Role and Mechanism of miRNA-1224 in the Polygonatum Sibiricum Polysaccharide Regulation of Bone Marrow-Derived Macrophages to Osteoclast Differentiation.

MicroRNAs (miRNAs) is an endogenous non-coding small molecule RNA that regulates cell proliferation, differentiation, fat metabolism and hormone secretion. Studies have shown that miRNAs regulate the processes related to osteoporosis, including the differentiation of osteoblasts, osteoclasts and chondrocytes, and are one of the important regulatory factors of some bone metabolic diseases. In our previous study has revealed that natural compound Polygonatum sibiricum polysaccharide (PSP) can promote osteoblast formation and blocking osteoclastogenesis through Wnt/β-catenin signalling pathway. This study was designed to investigate PSP can inhibits expression of osteoclast-related genes by Hippo signaling pathway, which was prevented by effectively blocking the expression of miR-1224. This study showed that there were 27 differentially expressed microRNAs when Polygonatum sibiricum polysaccharide inhibits osteoclastogenesis, the most notable of which was miR-1224. Furthermore, the study showed that PSP increased the level of Limd1 which was the target gene of miR-1224. In conclusion, these findings demonstrate that PSP suppressed osteoclastogenesis in vitro via Hippo signaling pathway based on miR-1224. This study may aid in the development of a therapeutic approach utilizing PSP for the enhancement of bone health and prevention of osteoporosis.