Draft genome sequence of human-associated streptogramin resistant Staphylococcus aureus.

OBJECTIVES: Staphylococcus aureus is one of the leading causes of nosocomial and community-acquired infections. Treatment of those infections with macrolide-lincosamide-streptogramin (MLS) antibiotics has led to resistance to those antibiotics through various mechanisms. S. aureus strain CIP108540, isolated from a human in France, has been previously shown to exhibit resistance to the streptogramins, Quinupristin and Dalfopristin; the presence of streptogramin resistance genes was verified by PCR. However, the extent of MLS resistance genes in this strain is unknown. This study analyzed the genome sequence of S. aureus CIP108540 to assess genes associated with resistance, including streptogramins.

METHODS: Genomic DNA of S. aureus CIP108540 was sequenced using Illumina MiSeq. The generated sequencing reads were de novo assembled using A5 miseq.

RESULTS: The draft genome size was 3, 014, 273bp with 32.72% GC content. The predicted coding sequences were 3,063 with 59 tRNA. Several antibiotic resistance genes were identified conferring resistance to various antibiotics.

CONCLUSION: The draft genome sequence of S. aureus CIP 108540 released here will provide valuable information for a better understanding of its genetic makeup and resistome.