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Monitoring of Fusarium trichothecenes in Canadian cereal grain shipments from 2010 to 2012.
Journal of Agricultural and Food Chemistry 2013 July 32
A method involving dry grinding, rotary sample dividing, and gas chromatography-mass spectrometry was evaluated for the analysis of eight Fusarium trichothecenes in cereal grains. Processing of whole cereal grains by the method produced representative test portions for the analysis of deoxynivalenol (DON). Method validation data, as well as the successful participation in various international proficiency tests, demonstrated the analytical method produced accurate and precise results. The evaluated method was used to monitor DON, 3- and 15-acetyldeoxynivalenol, nivalenol (NIV), T-2 toxin, HT-2 toxin, diacetoxyscirpenol, and fusarenon-X in shipments of Canadian wheat, durum, barley, corn, rye, and oats transported between August 1, 2010, and July 31, 2012. DON was the most frequently measured trichothecene, found in 231 of the 303 samples at concentrations up to 2.34 mg/kg; NIV was the next most frequently observed trichothecene, but its occurrence was limited to barley. Concentrations of DON were significantly associated with wheat class and grade. The median DON concentration in durum (0.09 mg/kg) was lower than that for hard red spring (0.21 mg/kg). Lower grades of wheat also contained higher median concentrations of DON than higher grades, supporting the current use of Fusarium damaged kernels as a grading factor to manage DON.
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