Assessment of polycyclic aromatic hydrocarbon biodegradation potential in mangrove sediment from Don Hoi Lot, Samut Songkram Province, Thailand

C Muangchinda, R Pansri, W Wongwongsee, O Pinyakong
Journal of Applied Microbiology 2013, 114 (5): 1311-24

AIMS: To assess the biodegradation potential of mixed polycyclic aromatic hydrocarbons (PAHs) in mangrove sediments.

METHODS AND RESULTS: Sediment microcosms were constructed with sediment collected from Don Hoi Lot, Samut Songkram Province, Thailand, by supplementation with a mixture of acenaphthene, phenanthrene and pyrene. At the end of 8 weeks, low molecular weight PAHs, acenaphthene and phenanthrene were completely degraded. PCR-denaturing gradient gel electrophoresis profile suggests that Marinobacter, Enterobacter and Dethiosulfatibacter play important roles in PAH degradation in mangrove sediment. Furthermore, six PAH-degrading bacteria were isolated consisting of novel phenanthrene-degrading Dyella sp. and Luteibacter sp., phenanthrene-degrading Burkholderia sp., acenaphthene-degrading Alcaligenes sp. and pyrene-degrading Ochrobactrum sp. Moreover, dioxygenase genes could be detected both in sediment microcosms as well as in all of the isolated strains.

CONCLUSIONS: These results demonstrated that indigenous bacteria in the mangrove sediment had the ability to degrade phenanthrene in the presence of mixture PAHs with high efficacy.

SIGNIFICANCE AND IMPACT OF THE STUDY: Culture and nonculture methods were combined to assess PAH biodegradation in mangrove sediment. Two novel phenanthrene-degrading bacteria were isolated. Three genera of bacteria that play important roles in PAH degradation were indicated by nonculture approach. Moreover, dioxygenase genes could be detected. This information is useful for further bioremediation of PAH-contaminated mangrove sediments.

Full Text Links

Find Full Text Links for this Article


You are not logged in. Sign Up or Log In to join the discussion.

Related Papers

Remove bar
Read by QxMD icon Read

Save your favorite articles in one place with a free QxMD account.


Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"