journal
https://read.qxmd.com/read/38419296/endophytic-streptomyces-sp-msare05-isolated-from-roots-of-peanut-plant-produces-a-novel-antimicrobial-compound
#1
JOURNAL ARTICLE
Md Majharul Islam, Shrabani Saha, Prithidipa Sahoo, Sukhendu Mandal
AIM: This study aimed to isolate, endophytic Streptomyces sp. MSARE05 isolated from root of a peanut (Arachis hypogaea) inhibits the growth of other bacteria. The research focused on characterizing the strain and the antimicrobial compound. METHODS AND RESULTS: The surface sterilized peanut roots were used to isolate the endophytic bacterium Streptomyces sp. MSARE05. A small-scale fermentation was done to get the antimicrobial compound SM05 produced in highest amount in ISP-2 medium (pH 7) for 7 days at 30°C in shaking (180 rpm) condition...
February 28, 2024: Journal of Applied Microbiology
https://read.qxmd.com/read/38419289/a-polyphasic-molecular-approach-to-characterize-a-collection-of-grapevine-endophytic-bacteria-with-bioprotective-potential
#2
JOURNAL ARTICLE
Giulia Bertazzoli, Luca Nerva, Walter Chitarra, Fabio Fracchetti, Ilenia Campedelli, Loredana Moffa, Marco Sandrini, Tiziana Nardi
AIMS: The work presented here was conducted to characterize the biodiversity of a collection of bacterial isolates, mainly wood endophytes, as part of a research project focused on exploring their bioprotective potential for postharvest biological control of fruits. METHODS AND RESULTS: This work was the basis for the development of a tailored method combining 16S rDNA sequencing and Rep-PCR to differentiate the isolates and identify them to genus level or below...
February 28, 2024: Journal of Applied Microbiology
https://read.qxmd.com/read/38409849/the-use-of-salmonid-epithelial-cells-to-characterize-the-toxicity-of-tenacibaculum-maritimum-soluble-extracellular-products
#3
JOURNAL ARTICLE
Matthew L Michnik, Shawna L Semple, Reema N Joshi, Patrick Whittaker, Daniel R Barreda
AIMS: This study assessed how the etiological agent of mouth rot in farmed Atlantic salmon, Tenacibaculum maritimum, induces toxicity in host salmonid barrier cells, and determined if environmental changes are relevant for these effects. METHODS AND RESULTS: T. maritimum soluble extracellular products (ECPs) were collected and used to treat Atlantic salmon and rainbow trout intestinal barrier cell lines as a comparative model of bacterial-salmonid cell interactions...
February 26, 2024: Journal of Applied Microbiology
https://read.qxmd.com/read/38389225/lipopeptides-from-bacillus-velezensis-induced-apoptosis-like-cell-death-in-the-pathogenic-fungus-fusarium-concentricum
#4
JOURNAL ARTICLE
Meichun Chen, Yingjie Deng, Meixia Zheng, Rongfeng Xiao, Xun Wang, Bo Liu, Jin He, Jieping Wang
AIMS: Stem rot caused by Fusarium concentricum is a new disease of Paris polyphylla reported by our research group. The present study investigates the growth inhibitory and apoptotic effects of Bacillus velezensis FJAT-54560 lipopeptide against F. concentricum. METHODS AND RESULTS: HPLC preparation and LC-MS analysis results show that the crude lipopeptides secreted by Bacillus velezensis FJAT-54560 isolated from Jasminum sambac consist of C14-17 iturin A, C14 fengycin B, C16 fengycin A/A2, C18 fengycin A, C20 fengycin B2, C21 fengycin A2, C22-23 fengycin A, C12-16 surfactin A, and C15 surfactin A derivatives...
February 22, 2024: Journal of Applied Microbiology
https://read.qxmd.com/read/38383865/rapid-detection-of-viable-microbes-with-ctc-and-cfda-using-a-fibre-fluorescence-spectroscopy-system
#5
JOURNAL ARTICLE
Jessica Chiang, Julia Robertson, Cushla McGoverin, Simon Swift, Frédérique Vanholsbeeck
AIMS: To assess the efficacy of two commercially available viability dyes, 5-cyano-2,3-di-(p-tolyl)tetrazolium chloride (CTC) and 5(6)-carboxyfluorescein diacetate (CFDA), in reporting on viable cell concentration and species using an all-fibre fluorometer. METHODS AND RESULTS: Four bacterial species (two Gram-positive and two Gram-negative) commonly associated with food poisoning or food spoilage (Escherichia coli, Salmonella enterica, Staphylococcus aureus, and Bacillus cereus) were stained with CTC or CFDA and the fibre fluorometer was used to collect full fluorescence emission spectra...
February 21, 2024: Journal of Applied Microbiology
https://read.qxmd.com/read/38383848/a-novel-bioinformatic-method-for-the-identification-of-antimicrobial-peptides-in-metagenomes
#6
JOURNAL ARTICLE
Julianne Megaw, Timofey Skvortsov, Giulia Gori, Aliyu I Dabai, Brendan F Gilmore, Christopher C R Allen
AIMS: This study aimed to develop a new bioinformatic approach for the identification of novel antimicrobial peptides (AMPs), which did not depend on sequence similarity to known AMPs held within databases, but on structural mimicry of another antimicrobial compound, in this case an ultrashort, synthetic, cationic lipopeptide (C12-OOWW-NH2). METHODS AND RESULTS: When applied to a collection of metagenomic datasets, our outlined bioinformatic method successfully identified several short (8-10aa) functional AMPs, the activity of which was verified via disk diffusion and minimum inhibitory concentration (MIC) assays against a panel of 12 bacterial strains...
February 21, 2024: Journal of Applied Microbiology
https://read.qxmd.com/read/38383817/phenotypic-virulence-characterization-of-avian-pathogenic-escherichia-coli-apec-isolates-from-broiler-breeders-with-colibacillosis-in-mississippi
#7
JOURNAL ARTICLE
Jiddu Joseph, Christopher Magee, Linan Jia, Li Zhang, Pratima Adhikari, Reshma Ramachandran
AIMS: In this study, we evaluated the phenotypic virulence characteristics of avian pathogenic Escherichia coli (APEC) isolates from broiler breeders with colibacillosis in Mississippi. Also, the relationship between phenotypic and genotypic virulence patterns was determined. METHODS AND RESULTS: Twenty-eight APEC isolated from lesions of broiler breeders diagnosed with colibacillosis were used for embryo lethality assay and chick challenge study. The percentage of embryo mortality following embryo lethality assay and pathogenicity score following the chick challenge study were used to categorize the isolates based on virulence...
February 21, 2024: Journal of Applied Microbiology
https://read.qxmd.com/read/38383758/the-synergic-and-addictive-activity-of-biogenic-silver-nanoparticle-bio-agnp-associated-with-meropenem-against-carbapenem-resistant-acinetobacter-baumannii
#8
JOURNAL ARTICLE
Suzane Olachea Allend, Marcelle de Oliveira Garcia, Kamila Furtado Cunha, Déborah Trota Faria Albernaz, Luciano Aparecido Panagio, Gerson Nakazaro, Guilherme Fonseca Reis, Thaís Larré Oliveira, Amilton Clair Pinto Seixas Neto, Daiane Drawanz Hartwig
AIMS: Antibiotic management of infections caused by Acinetobacter baumannii often fails due to antibiotic resistance (especially to carbapenems) and biofilm-forming strains. Thus, the objective here was to evaluate in vitro the antibacterial and antibiofilm activity of biogenic silver nanoparticle (Bio-AgNP) combined with meropenem, against multidrug-resistant (MDR) isolates of A. baumannii. METHODS AND RESULTS: In this study, A. baumannii ATCC® 19606™ and four carbapenem-resistant A...
February 21, 2024: Journal of Applied Microbiology
https://read.qxmd.com/read/38373840/enhancement-of-microbicidal-efficacy-of-chemical-disinfectants-when-combined-with-ultrasound-technology
#9
JOURNAL ARTICLE
Antonio Biasi, Mirella Gionta, Federica Pisa, Maura Pizzuti, Andrea Sortino, Alessio Immesi, Jodi R Alt, Imma Zampaglione
AIMS: This study investigated the antimicrobial efficacy of ultrasound technology (US) in combination with two different disinfectants (Disinfectant A and Disinfectant B), containing Peracetic Acid (PAA) and Quaternary Ammonium Compounds (QACs), respectively, against two sporigenic pathogens, Aspergillus brasiliensis and Bacillus subtilis. METHODS AND RESULTS: The microbicidal activity of the coupled treatment was compared with the use of the disinfectants alone, and the efficacy of the disinfection strategies was evaluated by the log reduction of the population of the microorganism inoculated onto stainless-steel surface...
February 19, 2024: Journal of Applied Microbiology
https://read.qxmd.com/read/38373822/could-giant-viruses-be-considered-as-a-biotechnological-tool-for-preventing-and-controlling-acanthamoeba-infections
#10
JOURNAL ARTICLE
Ana Paula Correia Crispim, Mateus Sá Serafim Magalhães, Adriana Oliveira Costa, Jônatas Santos Abrahão
AIM: The aim of the study was to evaluate the efficiency of mimivirus as a potential therapeutic and prophylactic tool against Acanthamoeba castellanii, the etiological agent of Acanthamoeba keratitis, a progressive corneal infection, which is commonly associated with the use of contact lenses and can lead to blindness if not properly treated. METHODS AND RESULTS: mimivirus particles were tested in different multiplicity of infection, along with commercial multipurpose contact lenses' solutions, aiming to assess their ability to prevent encystment and excystment of A...
February 19, 2024: Journal of Applied Microbiology
https://read.qxmd.com/read/38373804/causal-agents-of-stemphylium-induced-foliar-diseases-of-tomatoes-and-other-solanaceae-hosts-in-brazil
#11
JOURNAL ARTICLE
Tiago Bezerra Torres, Leonardo Silva Boiteux, David Nataren Perdomo, Josiene Silva Veloso, Maria Esther de Noronha Fonseca, Mariana Rodrigues Fontenelle, Ailton Reis
AIM: An extensive survey was done to clarify the prevalent Stemphylium species on Solanaceae plants across Brazil, and their host ranges. METHODS AND RESULTS: Eighty nine (89) Stemphylium isolates were obtained from naturally infected tomatoes as well as S. paniculatum, potato, eggplant, scarlet eggplant (S. aethiopicum var. gilo), Physalis angulata, and Capsicum species. Phylogenetic analyzes encompassing the ITS-5.8S rDNA and glyceraldehyde-3-phosphate dehydrogenase genomic regions placed the isolates into two distinct groupings with either S...
February 19, 2024: Journal of Applied Microbiology
https://read.qxmd.com/read/38366933/development-characterisation-and-evaluation-of-a-simple-polymicrobial-colony-biofilm-model-for-testing-of-antimicrobial-wound-dressings
#12
JOURNAL ARTICLE
Shaun N Robertson, Manuel Romero, Samuel Fenn, Petra L Kohler Riedi, Miguel Cámara
AIMS: Simple biofilm models are the first step to testing of any antimicrobial and wound dressing, therefore the aim of this study was to develop and validate a simple polymicrobial colony biofilm wound model comprised of Pseudomonas aeruginosa, Staphylococcus aureus and Candida albicans on RPMI-1640 agar. The model was then used to evaluate the topical disinfectant chlorohexidine and four commercially available wound dressings using the polymicrobial model. The model used was as a starting point to mimic debridement in clinical care of wounds and the effectiveness of wound dressings evaluated afterwards...
February 16, 2024: Journal of Applied Microbiology
https://read.qxmd.com/read/38364306/genome-guided-purification-of-high-amounts-of-the-siderophore-ornibactin-and-detection-of-potentially-novel-burkholdine-derivatives-produced-by-burkholderia-catarinensis-89t
#13
JOURNAL ARTICLE
Evelise Bach, Julia Chen, Célio Fernando Figueiredo Angolini, Judith S Bauer, Harald Gross, Luciane Maria Pereira Passaglia
AIM: The increased availability of genome sequences has enabled the development of valuable tools for the prediction and identification of bacterial natural products. Burkholderia catarinensis 89T produces siderophores and an unknown potent antifungal metabolite. The aim of this work was to identify and purify natural products of B. catarinensis 89T through a genome-guided approach. MATERIALS AND METHODS: The analysis of B. catarinensis 89T genome revealed 16 clusters putatively related to secondary metabolism and antibiotics production...
February 13, 2024: Journal of Applied Microbiology
https://read.qxmd.com/read/38364303/functional-differences-of-cultivable-leaf-associated-microorganisms-in-the-native-andean-tree-gevuina-avellana-mol-proteaceae-exposed-to-atmospheric-contamination
#14
JOURNAL ARTICLE
Alejandra Fuentes-Quiroz, Héctor Herrera, Roxana Alvarado, Claudia Rabert, Cesar Arriagada, Rafael Borges da Silva Valadares
AIMS: This study aimed to evaluate and describe the functional differences of cultivable bacteria and fungi inhabiting the leaves of Gevuina avellana Mol. (Proteaceae) in an urban area with high levels of air pollution and in a native forest in the southern Andes. METHODS AND RESULTS: Phyllosphere microorganisms were isolated from the leaves of G. avellana, their plant growth promoting capabilities were estimated along with their biocontrol potential and tolerance to metal(loid)s...
February 13, 2024: Journal of Applied Microbiology
https://read.qxmd.com/read/38346864/co-occurrence-of-blandm-1-and-blaoxa-23-in-carbapenemase-producing-acinetobacter-baumannii-belonging-to-high-risk-lineages-isolated-from-burn-patients-in-tunisia
#15
JOURNAL ARTICLE
Anis Raddaoui, Aymen Mabrouk, Yosra Chebbi, Siwar Frigui, Abbassi Mohamed Salah, Wafa Achour, Lamia Thabet
AIMS: Carbapenem-resistant Acinetobacter baumannii (CR-Ab) is an important cause of infections in burn patients. This study aimed to characterize the antimicrobial susceptibility pattern of CR-Ab isolated from burns in Burn Intensive Care Unit (BICU) of the Trauma and Burn Centre of Ben Arous, to determine the prevalence of β-lactamase-encoding genes and to search eventual genetic relatedness of CR-Ab strains. METHODS AND RESULTS: From 15th December 2016 to 2nd April 2017, all non-duplicated CR-Ab isolated in burn patients in the BICU were screened by simplex PCR for the class A, B, C and D β-lactamase genes...
February 12, 2024: Journal of Applied Microbiology
https://read.qxmd.com/read/38346851/efficacy-of-ergosterol-peroxide-obtained-from-the-endophytic-fungus-acrophialophora-jodhpurensis-against-rhizoctonia-solani
#16
JOURNAL ARTICLE
Zoha Daroodi, Parissa Taheri, Saeed Tarighi, Mehrdad Iranshahi, Maryam Akaberi
AIM: To investigate antifungal activity of the extract and major metabolite of the endophytic fungus Acrophialophora jodhpurensis (belonging to Chaetomiaceae) against crown and root rot caused by Rhizoctonia solani (teleomorph: Thanatephorus cucumeris), as an important pathogen of tomato. METHODS AND RESULTS: The endophytic fungus A. jodhpurensis, has high inhibitory effect against R. solani AG4-HG II in vitro and in vivo. The media conditions were optimized for production of the endophyte's metabolites...
February 12, 2024: Journal of Applied Microbiology
https://read.qxmd.com/read/38346849/an-integrated-method-for-targeted-oxford-nanopore-sequencing-and-automated-bioinformatics-for-the-simultaneous-detection-of-bacteria-fungi-and-arg
#17
JOURNAL ARTICLE
Sanjana Kuruwa, Amrutraj Zade, Sanchi Shah, Rameez Moidu, Shailesh Lad, Chhaya Chande, Ameeta Joshi, Nilma Hirani, Chaitali Nikam, Sanjay Bhattacharya, Aruna Poojary, Mahua Kapoor, Kiran Kondabagil, Anirvan Chatterjee
BACKGROUND: Metagenomics of clinical samples and microbiomes have enabled a better understanding of the role of pathogens. However, the use of metagenomics in clinical practice has been limited due to non-standardized methods of sample processing and need of expertise for the bioinformatic analysis of next generation sequencing (NGS) data. AIM: Here we describe a workflow to encourage the clinical utility and potential of NGS for the screening of bacteria, fungi and antimicrobial resistance genes (ARGs)...
February 12, 2024: Journal of Applied Microbiology
https://read.qxmd.com/read/38341278/heat-inactivation-of-aqueous-viable-norovirus-and-ms2-bacteriophage
#18
JOURNAL ARTICLE
Marlee Shaffer, Kimberly Huynh, Verónica Costantini, Jan Vinjé, Kyle Bibby
AIMS: This study aimed to compare the heat inactivation kinetics of viable human norovirus with the surrogate, MS2 bacteriophage as well as assess the decay of the RNA signal. METHODS AND RESULTS: Human intestinal enteroids (HIEs) were used to analyze the heat inactivation kinetics of viable human norovirus compared to the surrogate MS2 bacteriophage, which was cultured using a plaque assay. Norovirus decay rates were 0.22 min-1, 0.68 min-1, and 1.11 min-1 for 50°C, 60°C, and 70°C, respectively, and MS2 bacteriophage decay rates were 0...
February 10, 2024: Journal of Applied Microbiology
https://read.qxmd.com/read/38341275/siderophore-of-pgpr-origin-reduces-ros-mediated-injury-in-solanum-spp-caused-by-fungal-pathogens
#19
JOURNAL ARTICLE
Ravinsh Kumar, Ashutosh Singh, Ekta Shukla, Pratika Singh, Azmi Khan, Naveen Kumar Singh, Amrita Srivastava
AIMS: The study aims to explore antifungal properties of bacillibactin siderophore produced by the plant growth promoting rhizobacterium (PGPR) Bacillus subtilis against fungal phytopathogens Alternaria porri and Fusarium equiseti isolated from Solanum lycopersicum and Solanum melongena plants. METHODS AND RESULTS: A. porri and F. equiseti were isolated from infected plants of eggplant and tomato respectively. A plate assay was employed to assess the effect of bacillibactin against the phytopathogens...
February 10, 2024: Journal of Applied Microbiology
https://read.qxmd.com/read/38341274/laboratory-domestication-of-lactiplantibacillus-plantarum-alters-some-phenotypic-traits-but-causes-non-novel-genomic-impact
#20
JOURNAL ARTICLE
Sarvesh V Surve, Dasharath B Shinde, Joyleen M Fernandes, Sharoni Sharma, Monty Vijayvargiya, Komal Kadam, Ram Kulkarni
AIMS: Laboratory domestication has been negligibly examined in lactic acid bacteria (LAB). Lactiplantibacillus plantarum is a highly studied and industrially relevant LAB. Here, we passaged L. plantarum JGR2 in a complex medium to study the effects of domestication on the phenotypic properties and the acquisition of mutations. METHODS AND RESULTS: L. plantarum JGR2 was passaged in mMRS medium (deMan Rogossa Sharpe supplemented with 0.05% w/v L-cysteine) in three parallel populations for 70 days...
February 10, 2024: Journal of Applied Microbiology
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