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Journal of Applied Microbiology

Adriane Del Fabro Ceccin, Ana Paula Duarte Souza, Gabriel Tardin Hilário, Douglas Matheus Muller, Pedro Roosevelt Torre Romão, Luiz Carlos Rodrigues Junior
AIMS: The objective of this research was to quantify the levels of circulating HspBP1 and anti-HspBP1 IgG in HIV-infected individuals and to correlate them with CD4 T cell counts and viral load, as well as to determine the kinetics of those proteins during acute phase. METHODS AND RESULTS: Sixty serum samples from HIV-positive outpatients, thirty with high viral load and thirty with low viral load were analyzed. The HspBP1and anti-HspBP1 were quantified by ELISA...
February 20, 2019: Journal of Applied Microbiology
Xing Gao, Ke Xu, Guilian Yang, Chunwei Shi, Haibin Huang, Jianzhong Wang, Wentao Yang, Jing Liu, Qiong Liu, Yuanhuan Kang, Yanlong Jiang, Chunfeng Wang
AIMS: Genotype VII Newcastle disease (ND) is one of the most epidemic and serious infectious diseases in the poultry industry. A novel vaccine targeting VII NDV is still in requirement. METHODS AND RESULTS: In this study, we constructed recombinant regulate delayed lysis Salmonella strains expressing either fusion protein (F) alone under eukaryotic CMV promoter or together with chicken IL-18 (chIL-18) as a molecular adjuvant under prokaryotic Ptrc promoter, named pYL1 and pYL23, respectively...
February 20, 2019: Journal of Applied Microbiology
Srikanth Katla, Bappa Karmakar, Subbi Rami Reddy Tadi, Naresh Mohan, B Anand, Uttariya Pal, Senthilkumar Sivaprakasam
AIMS: The present study was aimed at design of experiments (DoE) and artificial intelligence based culture medium optimization for high level extracellular production of a novel recombinant human interferon alpha 2b (huIFNα2b) in glycoengineered Pichia pastoris and its characterization. METHODS AND RESULTS: Artificial neural network- Genetic algorithm (ANN-GA) model exhibited improved huIFNα2b production and better predictability compared to RSM. The optimized medium exhibited 5-fold increase in huIFNα2b titer compared to the complex medium...
February 18, 2019: Journal of Applied Microbiology
C V Robinson, A H Bishop
AIMS: To develop a gel formulation to trigger a visual signal for rapid disclosure of the location and extent of surface contamination with viable Bacillus anthracis spores. METHODS AND RESULTS: Methylumbelliferyl -α-D-glucopyranoside was combined with hyaluronic acid to produce a gel that could be applied to a surface as a coating. It remained hydrated for a sufficient time for α-glucosidase activity present in intact B. anthracis spores to cleave the substrate and release the fluorescent product, methylumbelliferone...
February 18, 2019: Journal of Applied Microbiology
Rosalie A Multari, David A Cremers, Ann Nelson, Zohreh Karimi, Stephen Young, Carolyn Fisher, Robert Duncan
AIMS: To investigate the use of a laser-based method of detection as a potential diagnostic test for the rapid identification of infectious agents in human blood. METHODS AND RESULTS: In this study, the successful differentiation of blood spiked with viruses, bacteria, or protozoan parasites to clinically relevant levels is demonstrated using six blood types (O+, O-, AB+, A+, A-, B+) using blood from different individuals with blood samples prepared in two different laboratories...
February 15, 2019: Journal of Applied Microbiology
Sandra Kolbeck, Jürgen Behr, Rudi F Vogel, Christina Ludwig, Matthias A Ehrmann
AIMS: Coagulase negative Staphylococcus xylosus strains are used as starter organisms for sausage fermentation. As those strains have to cope with low pH-values during fermentation, the aim of this study was to identify the acid adaptation mechanisms of S. xylosus TMW 2·1523 previously isolated from salami. METHODS AND RESULTS: A comparative proteomic study between two different acid tolerant mutants was performed. Therefore, both S. xylosus mutants were grown pH-static under acid stress (pH 5·1) and reference conditions (pH 7·0)...
February 14, 2019: Journal of Applied Microbiology
Wilma Raianny Vieira da Rocha, Tales Alexandre Costa-Silva, Gualberto Segundo Agamez Montalvo, Valker Araújo Feitosa, Suellen Emilliany Feitosa Machado, Gláucia Manoela de Souza Lima, Adalberto Pessoa-Jr, Harley da Silva Alves
AIMS: The aim of this study was to find new eukaryotic sources of the L-asparaginase (L-ASNase), since the prokaryotic sources of the enzyme are well reported as causing allergic hypersensitivity reactions in a significant number of patients. This report describes screening for L-ASNase production by filamentous fungi isolated from the Brazilian Caatinga, and the optimization of fermentation parameters to increase fungal growth and improve yield in the production of L-ASNase. METHODS AND RESULTS: 32 filamentous fungi were investigated in this study...
February 14, 2019: Journal of Applied Microbiology
A Girardeau, C Puentes, S Keravec, P Peteuil, I C Trelea, F Fonseca
AIM: Investigate the effect of a broad spectrum of culture conditions on the acidification activity and viability of Carnobacterium maltaromaticum CNCM I-3298, the main technological properties that determine the shelf-life of biological Time-Temperature Integrator labels. METHODS AND RESULTS: Cells were cultivated at different temperatures (20 to 37 °C) and pH (6 to 9·5) according to a modified central composite design and harvested at increasing times up to 10 h of stationary phase...
February 14, 2019: Journal of Applied Microbiology
Maha Usama Abdelhaseib, Atul K Singh, Arun K Bhunia
AIM: To investigate the use of a light scattering sensor, BARDOT (BActerial Rapid Detection using Optical scattering Technology) coupled with a multi-pathogen selective medium, SEL (Salmonella, Escherichia, and Listeria) for concurrent detection of the three major foodborne pathogens in a single assay. METHODS AND RESULTS: BARDOT was used to detect and distinguish the three major pathogens, Salmonella enterica, Shiga-toxin producing Escherichia coli (STEC) and Listeria monocytogenes from food based on colony scatter signature patterns on SEL agar (SELA)...
February 14, 2019: Journal of Applied Microbiology
Kai Feng, Zigen Cai, Tao Ding, Hai Yan, Xiaolu Liu, Zhongbao Zhang
AIMS: The purpose of this study was to determine the positive effects of potassium-solubilizing bacteria and photosynthetic bacteria on the salt tolerance of maize. METHODS AND RESULTS: We selected the maize inbred lines USTB-265 (salt-sensitive), USTB-109 (moderately salt-tolerant), and USTB-297 (salt-tolerant) to investigate their growth characteristics, enzyme activity, and gene expression in response to inoculation with photosynthetic bacteria and potassium-solubilizing bacteria under salt-stress conditions...
February 13, 2019: Journal of Applied Microbiology
Ilzé Horak, Gerhard Engelbrecht, Peet J Jansen van Rensburg, Sarina Claassens
Root-knot nematodes are destructive phytopathogens that damage agricultural crops globally and there is growing interest in the use of biocontrol based on rhizobacteria such as Bacillus to combat Meloidogyne species. It is hypothesized that nematicidal activity of Bacillus can be attributed to the production of secondary metabolites and hydrolytic enzymes. Yet, few studies have characterized these metabolites and their identities remain unknown. Others are speculative or fail to elaborate on how secondary metabolites were detected or distinguished from primary metabolites...
February 10, 2019: Journal of Applied Microbiology
I A Rosa, P Rodrigues, A E Bianchini, B P Silveira, F T Ferrari, G Bandeira Junior, A P C Vargas, B Baldisserotto, B M Heinzmann
AIMS: This study investigated the in vitro antibacterial activity of Hesperozygis ringens (Benth.) Epling leaf extracts against fish pathogenic bacteria, as well as the in vivo activity of the most active extract in silver catfish (Rhamdia quelen) experimentally infected with Aeromonas hydrophila. Moreover, the chemical composition of the extract used in the survival assay was evaluated. METHODS AND RESULTS: Only hexane extract (HEHR) showed in vitro antibacterial activity (MIC and MBC ranging from 1600 to 3200 μg mL-1 ) against clinical isolates of A...
February 8, 2019: Journal of Applied Microbiology
Carolina Monteiro, Dolores Pinheiro, Marta Maia, Miguel A Faria, Catarina Lameiras, Eugénia Pinto
AIMS: Aspergillus spp. are ubiquitous saprophytic fungi and their conidia easily inhaled. This is particularly important in immunocompromised patients, more susceptible to developing invasive aspergillosis. In addition to A. fumigatus sensu stricto, cryptic species, many resistant to antifungal drugs, have been associated with invasive infections, making important to assess their presence and diversity in different environments. Therefore, the aim of this study was to assess the presence, diversity, and susceptibility to antifungal drugs of airborne fungi...
February 8, 2019: Journal of Applied Microbiology
S M E Bravo, M Morales, S M Del Mónaco, A C Caballero
AIMS: A culture medium based on apple bagasse was designed and tested as a substrate for biomass production of conventional and unconventional native wine yeasts. METHODS AND RESULTS: The physicochemical characterization of the apple bagasse was carried out and its potential utility as a constituent of a complete culture medium for the production of yeast biomass was analyzed using the Experimental Statistical Designs. Growth parameters of conventional and non-conventional Patagonian wine yeasts were analyzed with Placket-Burman designs and Response Surface Methodology (RSM), comparing in each assay the apple bagasse substrate with the commonly used substrate for biomass development, cane molasses...
February 7, 2019: Journal of Applied Microbiology
Meichun Chen, Jieping Wang, Yujing Zhu, Bo Liu, Wenjing Yang, Chuanqing Ruan
AIMS: The aims of this study were to identify the structure of antibacterial lipopeptide produced by Bacillus amyloliquefaciens strain FJAT-2349, to analyze the effects of the culture medium and temperature on lipopeptide production and to assess the biocontrol efficiency of the lipopeptides against tomato bacterial wilt. METHODS AND RESULTS: Lipopeptides were extracted by acid precipitation and resolved in methanol and their structure was identified through LC-QTOF-MS/MS method...
February 1, 2019: Journal of Applied Microbiology
Lei Li, Jinyun Li, Jing Peng, Wenliang Wu, Yanbin Guo
AIMS: Rahnella aquatilis is a gram-negative bacterial species with potential for agricultural and industrial applications, as well as a human pathogen. This study aims to identify an optimal reference gene to explore antibiotic-resistance and stress tolerance in R. aquatilis using reverse-transcription quantitative polymerase chain reaction (RT-qPCR). METHODS AND RESULTS: Expression levels of 14 housekeeping genes in R. aquatilis were estimated by RT-qPCR under six different conditions: exponential phase, stationary phase, acid, salinity, antibiotic, and oxidative stresses...
January 31, 2019: Journal of Applied Microbiology
Lilit Gabrielyan, Lilit Hakobyan, Ashkhen Hovhannisyan, Armen Trchounian
AIMS: Antibiotic resistance of different bacteria requires the development of alternative approaches for overcoming this phenomenon. The antibacterial effects of iron oxide (Fe3 O4 ) nanoparticles (NPs) (from 50 μg mL-1 to 250 μg mL-1 ) on Escherichia coli antibiotic-resistant strains have been aimed. METHODS AND RESULTS: The study was performed with ampicillin-resistant E. coli DH5α-pUC18 and kanamycin-resistant E. coli pARG-25 stains. Specific growth rate of bacteria (μ), lag phase duration and colony forming units (CFU) were determined to evaluate growth properties...
January 31, 2019: Journal of Applied Microbiology
Tolulope O Faniyi, Moyosore J Adegbeye, Mona M Y Elghandour, Alberto B Pilego, Abdelfattah Z M Salem, Taiwo A Olaniyi, Opeyemi Adediran, Michael K Adewumi
Alongside the liver, rumen is one of the most important components of metabolism in ruminants. However, the microbes residing in the rumen are influenced by several complex factors such as diet, which result in fluctuations in the rumen pH. Rumen pH, affects feed intake and feed digestibility, subsequently causing microbial shift in the individual members of microbial community residing in the foregut, hindgut. This in turn, causes increase in lipopolysaccharide concentration, among other factors in the gut fluid and animal blood...
January 29, 2019: Journal of Applied Microbiology
Martina Velasova, Richard P Smith, Fabrizio Lemma, Robert A Horton, Nick Duggett, Judith Evans, Sue C Tongue, Muna F Anjum, Luke Randall
AIMS: This study investigated the occurrence and genetic diversity of Enterobacteriaceae with Extended-Spectrum Beta-Lactamase (ESBL), AmpC and carbapenemase mediated resistance in British beef cattle, and related risk factors. METHODS AND RESULTS: Faecal samples (n=776) were obtained from farms in England & Wales (n=20) and Scotland (n=20) in 2015. Isolates from selective agars were identified by MALDI ToF mass spectrometry. Selected isolates were characterised by multiplex PCR (blaCTX -M, blaOXA , blaSHV and blaTEM genes), whole genome sequencing (WGS), MICs and PFGE...
January 28, 2019: Journal of Applied Microbiology
Imen Smida, Carola Pentelescu, Ovidiu Pentelescu, Alaa Sweidan, Nolwenn Oliviero, Vincent Meuric, Benedicte Martin, Loredana Colceriu, Martine Bonnaure-Mallet, Zohreh Tamanai-Shacoori
AIM: The purpose of this study was to conduct phytochemical analysis of sea buckthorn pulp oil and to evaluate the antimicrobial, anti-biofilm and antioxidant activities of its mouthwash form. METHODS AND RESULTS: Fatty acid composition of the sea buckthorn pulp oil was determined by GC-MS analysis, which revealed that, mono-unsaturated fatty acid, palmitoleic acid and saturated fatty acid, palmitic acid, were the major constituents. The antimicrobial and the anti-biofilm capacities of sea buckthorn pulp oil mouthwash form were evaluated against Streptococcus gordonii, Porphyromonas gingivalis, Actinomyces viscosus and Candida albicans, according to the European Norms, and the Biofilm Ring Test® , respectively...
January 23, 2019: Journal of Applied Microbiology
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