Determination of imperatorin in rat plasma by reversed-phase high-performance liquid chromatography after oral administration of Radix Angelicae dahuricae extract

Yubo Li
Journal of Pharmaceutical and Biomedical Analysis 2006 March 18, 40 (5): 1253-6
A simple high-performance liquid chromatographic (HPLC) method has been developed for the determination of imperatorin in rat plasma and applied to a pharmacokinetic study in rats after administration of Radix Angelicae dahuricae extract. After addition of fluocinonide as an internal standard (IS), plasma samples were extracted with diethyl ether. HPLC analysis of the extracts was performed on a Diamonsil C18 analytical column using methanol-water (70:30, v/v) as the mobile phase. The UV detector was set at 254 nm. The standard curve was linear over the range 0.04-4.0 microg/mL. The lower limit of quantification was 0.04 microg/mL. The HPLC method developed could be easily applied to the determination and pharmacokinetic study of imperatorin in rat plasma after giving the animals Radix Angelicae dahuricae extract.

Full Text Links

Find Full Text Links for this Article


You are not logged in. Sign Up or Log In to join the discussion.

Related Papers

Remove bar
Read by QxMD icon Read

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"