Journals Journal of Pharmaceutical and ...

Journal of Pharmaceutical and Biomedical Analysis
Miao Hou, Haitao Tian, Jun Wu, Zhipeng Deng
Pinocembrin-7-O-β-D-glucoside (PCBG) isolated from Penthorum chinense Pursh was proven to display a wide range of pharmacological effects including hepatoprotection, anti-hepatoma and antifungal activities, etc. The research aims to qualitatively analyze the metabolites of PCBG in rat plasma, urine, bile and feces, and further perform the excretion study of PCBG and its major metabolite pinocembrin (PCB). Fifteen rats were divided into three groups (n=5 for each group) for blood, bile, urine and feces collection, respectively...
June 6, 2024: Journal of Pharmaceutical and Biomedical Analysis
Xinyu Zhao, Caixia Li, Hongxia Zeng, Xiao Gu, Jinqi Zheng
There is an increasing scientific interest in the detection of genotoxic impurities (GTIs), with nitrobenzene compounds being considered potential genotoxic impurities due to their structural alerts, which demonstrates a threat to drug safety for patient. While current reports on the detection of nifedipine impurity primarily focus on general impurities in nifedipine. In this study, an effective and simple gas chromatography-mass spectrometry (GC-MS) method was established and verified for the separation and quantification of 2-nitrotoluene, 2-nitrobenzyl alcohol, 2-nitrobenzaldehyde, 3-nitrobenzaldehyde, 4-nitrobenzaldehyde, and 2-nitrobenzyl bromide in nifedipine, which have not been previously reported...
June 4, 2024: Journal of Pharmaceutical and Biomedical Analysis
Ensar Piskin, Ahmet Cetinkaya, Mehmet A Unal, Erdogan Özgür, Esen Bellur Atici, Lokman Uzun, Sibel A Ozkan
This study presented a new method to design a MIP-based electrochemical sensor that could improve the selective and sensitive detection of ipratropium bromide (IPR). The polymeric film was designed using 2-hydroxyethyl methacrylate (HEMA) as the basic monomer, 2-hydroxy-2-methylpropiophenone as the initiator, ethylene glycol dimethacrylate (EGDMA) as the crosslinking agent, and N-methacryloyl-L-aspartic acid (MAAsp) as the functional monomer. The presence of MAAsp results in the functional groups in imprinting binding sites, while the presence of poly(vinyl alcohol) (PVA) allows the generation of porous materials not only for sensitive sensing but also for avoiding electron transport limitations...
June 4, 2024: Journal of Pharmaceutical and Biomedical Analysis
Rongqi Lin, Yipeng Cai, Yingbin Huang, Xueyong Li, Yiying Chen, Bo Chen, Kaixian Lai, Junnan Wu, Yu Cheng, Maobai Liu, Yisheng Chen, Hongqiang Qiu
Sirolimus (SRL) is commonly used in transplant patients to prevent organ transplant rejection. The current guidelines recommend to perform SRL therapeutic drug monitoring regularly to improve treatment outcomes and avoid adverse effects. Consequently, a precise and accurate method for determining SRL is crucial in clinical practice. Currently, liquid chromatography-tandem mass spectrometry (LC-MS/MS) and immunoassays have been widely adopted for determining SRL concentrations. However, previous studies have shown that immunoassays exhibit a positive bias compared to LC-MS/MS...
June 3, 2024: Journal of Pharmaceutical and Biomedical Analysis
Ziyu Li, Min Zhao, Zheyong Wang, Lizhou Ma, Xuan Pan, Tong Jin, Zixuan Fu, Bo Yuan, Chunjie Zhao, Yumeng Zhang
Dingchuan Decoction (DCD) is a traditional Chinese medicine prescription commonly used in the treatment of asthma, but the mechanism of DCD in treating asthma has not yet been determined. In this study, we employed a combination of metabolomics and network pharmacology to investigate the mechanism of DCD in treating asthma. An allergic asthma rat model was induced by ovalbumin (OVA). Metabolomics based on 1 H NMR and UHPLC-MS was used to identify differential metabolites and obtain the major metabolic pathways and potential targets...
June 1, 2024: Journal of Pharmaceutical and Biomedical Analysis
Mengru Bai, Qian Shen, Yong Wu, Zhiyuan Ma, Yuqing Wang, Mingyang Chen, Dan Liu, Lin Zhou
Methotrexate (MTX) is commonly prescribed as the initial treatment for gestational trophoblastic neoplasia (GTN), but MTX monotherapy may not be effective for high-risk GTN and choriocarcinoma. The cellular uptake of MTX is essential for its pharmacological activity. Thus, our study aimed to investigate the cellular pharmacokinetics and transport mechanisms of MTX in choriocarcinoma cells. For the quantification of MTX concentrations in cellular matrix, a liquid chromatography-tandem mass spectrometry method was created and confirmed initially...
May 29, 2024: Journal of Pharmaceutical and Biomedical Analysis
Xue Cheng, Lu Cao, Xiongjie Sun, Shuhan Zhou, Tianxiang Zhu, Junping Zheng, Songlin Liu, Hongtao Liu
Poria cocos (Schw.) Wolf (PCW) are the dried sclerotia of Poaceae fungus Poria cocos that contain many biological activity ingredients such as polysaccharides and triterpenoids. The carbohydrates from Poria cocos have been proven to possess anti-inflammatory and antioxidant effects. This study aimed to investigate the impact and mechanism of Poria cocos oligosaccharides (PCO) protecting mice against acute lung injury (ALI). We examined the histopathological analysis of lung injury, inflammatory, and edema levels to evaluate the benefits of PCO during ALI...
May 29, 2024: Journal of Pharmaceutical and Biomedical Analysis
Adéla Böhmová, Miloš Mikoška, Kamila Syslová, Dominika Šindelářová, Petr Hříbek, Petr Urbánek, Vladimír Setnička
Hepatocellular carcinoma (HCC) is one of the leading causes of cancer-related deaths in the world. HCC is often diagnosed late because patients with early-stage cancer have no apparent symptoms. Therefore, it is desirable to find a reliable method for an early diagnosis based on the detection of metabolites - biomarkers, that can be detected in the early stages of the disease. Untargeted metabolomics is often used as a tool to find a suitable biomarker for several diseases. In this work, untargeted metabolomics was performed on blood plasma samples of HCC patients and compared with healthy individuals and patients with liver cirrhosis...
May 28, 2024: Journal of Pharmaceutical and Biomedical Analysis
Hani Alam, Gozde Ozesme Taylan, Cem Yamali, Mecit H Oztop
Diabetes patients often rely on tailored insulin therapies, necessitating precise blends of various insulin types to achieve optimal pharmacokinetic profiles, including the quantity and action duration of insulin absorption into the bloodstream. This study aimed to develop an accurate quantification method for mixed insulin preparations, consisting of Insulin-NPH and Insulin Regular in ratios varying between 0:100-100:0. Time Domain NMR (TD-NMR) techniques, T2 relaxation times, and T1 T2 maps were used to analyze the mixtures...
May 28, 2024: Journal of Pharmaceutical and Biomedical Analysis
Shangbin Li, Xiong Gao, Yiwei Han, Yankun Song, Wenhui Wu, Yuqing Fan, Changjun Ren, Ling Hao
Hyperbilirubinemia in newborns may progress to acute bilirubin encephalopathy (ABE), posing short- and long-term health risks. Despite extensive research identifying numerous mRNAs, lncRNAs, circRNAs, and miRNAs associated with brain injury, their roles in neonatal bilirubin-induced brain injury remain elusive. This study employed whole-transcriptome sequencing to ascertain the differentially expressed (DE) RNA profiles in a newborn ABE rat model, followed by bioinformatic analysis. A time-series competing endogenous RNA (ceRNA) regulatory network was established, and the expression trends of 9 arbitrarily chosen RNAs were verified through quantitative real-time polymerase chain reaction(qRT-PCR)...
May 27, 2024: Journal of Pharmaceutical and Biomedical Analysis
Yan Qi Tan, C-Khai Loh, Shamin Mohd Saffian, Suzana Makpol
Therapeutic drug monitoring of pegylated L-asparaginase (ASNase) ensures the drug effectiveness in childhood acute lymphoblastic leukaemia (ALL) patients. The biological drug property with variable immunogenic host clearance, and the prescription of its generic formulation urge the need for a reliable assay to ensure an optimal treatment and improve outcome. This study aimed to optimise an existing isocratic reversed-phase high performance liquid chromatography (RP-HPLC) method with an automated pre-column sample derivatisation and injection program, and a computational algorithm for measuring serum pegylated ASNase activity in children with ALL...
May 27, 2024: Journal of Pharmaceutical and Biomedical Analysis
Pelin Şenel, Abdullah Al Faysal, Taner Erdoğan, Mustafa Doğan, Ayşegül Gölcü
Pregabalin (PGB) is a γ-aminobutyric acid (GABA) alkylated analog prescribed to treat neuropathic pain, fibromyalgia, and postherpetic neuralgia. Using analytical, spectroscopic methods and molecular docking and molecular dynamics (MD) simulations, a detailed experimental and theoretical investigation was conducted into the binding process and interactions between PGB and double-stranded fish sperm deoxyribonucleic acid (dsDNA). It was evident from the collected experimental results that PGB binds with ds-DNA...
May 27, 2024: Journal of Pharmaceutical and Biomedical Analysis
J Ricardo Lucio-Gutiérrez, Paula Cordero-Pérez, José Luis Ávila-Velázquez, Liliana Torres-González, Iris C Farías-Navarro, Gustavo Govea-Torres, Concepción Sánchez-Martínez, Pedro A García-Hernández, Jordi Coello-Bonilla, Míriam Pérez-Trujillo, Teodor Parella, Noemí H Waksman-Minsky, Alma L Saucedo
Serum 1 H NMR metabolomics has been used as a diagnostic tool for screening type 2 diabetes (T2D) with chronic kidney disease (CKD) as comorbidity. This work aimed to evaluate 1 H NMR data to detect the initial kidney damage and CKD in T2D subjects, through multivariate statistical analysis. Clinical data and biochemical parameters were obtained for classifying five experimental groups using KDIGO guidelines: Control (healthy subjects), T2D, T2D-CKD-mild, T2D-CKD-moderate, and T2D-CKD-severe. Serum 1 H NMR spectra were recorded to follow two strategies: one based on metabolite-to-creatinine (Met/Cr) ratios as targeted metabolomics, and the second one based on untargeted metabolomics from the 1 H NMR profile...
May 26, 2024: Journal of Pharmaceutical and Biomedical Analysis
Audrey Chen, Jocelyn Chen, William E Acree
A polemic is given regarding several of the calculated curve-fit parameters that Zhou and coworkers reported in their published paper. The calculated curve-fit parameters for the Combined Nearly Ideal Binary Solvent/Redlich-Kister, Jouyban-Acree-van't Hoff, Sun and modified Apelblat models were found to give mole fraction solubilities that exceeded unity. Our analysis also found that the mean relative absolute percent deviations provided by the authors were significantly underestimated.
May 25, 2024: Journal of Pharmaceutical and Biomedical Analysis
Min Zhou, Wei Wu, Hong Pu, Feng Chen, Chaofan Yi, Yuande Long
A long-term stability study using high performance liquid chromatography (HPLC) revealed an unidentified impurity in the bromhexine hydrochloride injection, which was employed as a mucolytic agent. Investigations into stress degradation and elemental impurities revealed one of the elemental impurities Fe3+ in this injection as the primary generator of these impurities. This impurity, named N-carboxymethyl bromhexine, was a product formed during drug-excipient interaction between bromhexine and tartaric acid with Fe3+ ...
May 24, 2024: Journal of Pharmaceutical and Biomedical Analysis
Ram S Kaulgud, Nidish H B, Mahantesh M Kurjogi, Veeresh S, Gulamnabi L Vanti
Organophosphorus (OP) compounds are the most extensively used pesticides' class worldwide; cause most self‑poisoning deaths especially in India. Thus, it is utmost important for early identification and aggressive management of OP poisoning from the clinical perspective to prevent serious complications by using sophisticated LC-MS/MS approach. This was a prospective study involving 103 patients of OP cases admitted to Karnataka Institute of Medical Sciences from June 2022 to May 2023, based on the inclusion and exclusion criteria patients were subjected to study...
May 24, 2024: Journal of Pharmaceutical and Biomedical Analysis
Matthias Wolfgang, Tihana Baniček, Amrit Paudel, Heidrun Gruber-Woelfler, Martin Spoerk, Varun Kushwah, Johannes G Khinast
Film-coated modified-release tablets are an important dosage form amenable to targeted, controlled, or delayed drug release in the specific region of the gastrointestinal (GI) tract. Depending on the film composition and interaction with the GI fluid, such coated products can modulate the local bioavailability, systemic absorption, protection as an enteric barrier, etc. Although the interaction of a dosage form with the surrounding dissolution medium is vital for the resulting release behavior, the underlying physicochemical phenomena at the film and core levels occurring during the drug release process have not yet been well described...
May 24, 2024: Journal of Pharmaceutical and Biomedical Analysis
De-Jian Chen, Cheng-Jun Liu, Zi-Hao Chen, Jin-Jin Li, Wei Shi, Qing-Min Zhang, Xia Yang, Jian-Xin Chen, Feng-Xiang Zhang
Zhi-Ke-Bao pills (ZKB), a traditional Chinese medicine preparation composed of 13 herbs, is generally used to treat cough caused by external wind cold, phlegm, etc in clinical applications, and it plays a core role in relieving cough caused by COVID-19 and influenza in China. Till now, the understanding of its chemical constituents was dramatically limited due to its chemical complexity, restricting its clinical application or development. In this work, a developed ultra-high performance liquid chromatography coupled to quadrupole time-of-flight mass spectrometry (UPLC-Q/TOF MS) method, a targeted and non-targeted strategy and network pharmacology were used to comprehensively characterize the chemical compositions in ZKB and predict its mechanism against cough...
May 24, 2024: Journal of Pharmaceutical and Biomedical Analysis
Marta Leite, Andreia Freitas, Todd Mitchell, Jorge Barbosa, Fernando Ramos
Consumption of misidentified foraged mushrooms containing bicyclic amanitin octapeptides is a worldwide public health and veterinary problem, being considered one of the deadliest accidental human and canine food ingestion due to acute liver failure (ALF). Reversal of advanced ALF and complete clinical recovery can be achieved following definitive removal of accumulated amatoxin laden bile from the gallbladder. An accurate means of quantifying amanitin content in aspirated bile is, therefore, urgently needed...
May 24, 2024: Journal of Pharmaceutical and Biomedical Analysis
Eduardo G de Campos, David G Farrar, Alex J Krotulski
Synthetic cannabinoids are a class of novel psychoactive substances that emerged in the drug market in the early 2010s. Since then, a wide range of different synthetic cannabinoids has been detected in drug materials and in biological specimens collected from intoxication cases. In general, synthetic cannabinoids are reported first in seized materials. In this study, the identification of the novel synthetic cannabinoid, ADB-5'Br-BINACA is reported. A plant material suspected to contain a synthetic cannabinoid was extracted and analyzed...
May 23, 2024: Journal of Pharmaceutical and Biomedical Analysis
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