Ye Yang, Anne P Beigneux, Wenxin Song, Le Phuong Nguyen, Hyesoo Jung, Yiping Tu, Thomas A Weston, Caitlyn M Tran, Katherine Xie, Rachel G Yu, Anh P Tran, Kazuya Miyashita, Katsuyuki Nakajima, Masami Murakami, Yan Q Chen, Eugene Y Zhen, Joonyoung R Kim, Paul H Kim, Gabriel Birrane, Peter Tontonoz, Michael Ploug, Robert J Konrad, Loren G Fong, Stephen G Young
Why apolipoprotein AV (APOA5) deficiency causes hypertriglyceridemia has remained unclear, but we have suspected that the underlying cause is reduced amounts of lipoprotein lipase (LPL) in capillaries. By routine immunohistochemistry, we observed reduced LPL staining of heart and brown adipose tissue (BAT) capillaries in Apoa5-/- mice. Also, after an intravenous injection of LPL-, CD31-, and GPIHBP1-specific mAbs, the binding of LPL Abs to heart and BAT capillaries (relative to CD31 or GPIHBP1 Abs) was reduced in Apoa5-/- mice...
December 1, 2023: Journal of Clinical Investigation