Evan A Clark, Edward Ryan R Talatala, Wenda Ye, Ruth J Davis, Samuel L Collins, Alexander T Hillel, Marisol Ramirez-Solano, Quanhu Sheng, Celestine N Wanjalla, Simon A Mallal, Alexander Gelbard
OBJECTIVES: Recent translational scientific efforts in subglottic stenosis (SGS) support a disease model where epithelial alterations facilitate microbiome displacement, dysregulated immune activation, and localized fibrosis. Given the observed immune cell infiltrate in SGS, we sought to test the hypothesis that SGS cases possessed a low diversity (highly clonal) adaptive immune response when compared with healthy controls. METHODS: Single cell RNA sequencing (scRNA-seq) of subglottic mucosal scar in iSGS (n = 24), iLTS (n = 8), and healthy controls (n = 7) was performed...
October 3, 2023: Laryngoscope