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Ultrasonic Bath

Atefeh Pesaraklou, Nasser Mahdavi-Shahri, Halimeh Hassanzadeh, Masoud Ghasemi, Mahboubeh Kazemi, Nasser Sanjar Mousavi, Maryam M Matin
Today advancements in nanotechnology have made extensive progress in tissue engineering. Application of cerium oxide nanoparticles (CeO2) has improved regenerative medicine due to their antioxidant properties. In this study, nanoparticles were used to increase the efficacy of skin substitutes. Human skin samples were decellularized using four methods and studied via histological stainings and DNA content analyses. Then CeO2 dispersing and its stability were investigated. The prepared acellular dermal matrices (ADMs) were immersed in CeO2 suspension and their effects were evaluated on growth of cultured human adipose derived-mesenchymal stem cells (hAd-MSCs) using MTT assay and histological methods...
February 12, 2019: Biomedical Materials
Pey-Shiuan Wu, Yu-Syuan Li, Yi-Ching Kuo, Suh-Jen Jane Tsai, Chih-Chien Lin
Resveratrol ( tran -3,5,4'-trihydroxystibene, RSV) is a kind of polyphenol which has anti-inflammatory, antioxidant, anti-allergy, and anti-cancer properties, as well as being a scavenger of free radicals and preventing cardiovascular diseases. However, it is quite unstable in light, heat, and other conditions, and decays easily due to environmental factors. For these reasons, this study used a new type of carrier, transfersome, to encapsulate RSV. Transfersome consists of phosphatidyl choline (PC) from a liposomal system and non-ionic edge activators (EA)...
February 8, 2019: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
Abdellatif Moujahid, John J Bang, Fei Yan
Herein, we report the comparison of two different mixing methods for reductive dechlorination of gamma-hexachlorocyclohexane (γ-HCH), aldrin, and p, p'-dichlorodiphenyl-trichloroethane (p, p'-DDT), using iron/palladium (Fe/Pd) bimetallic nanoparticles. A noticeable enhancement of the reaction rate was found when the reductive dechlorination reaction was carried out in an ultrasound bath as compared with a platform shaker. These enhancements could be attributed to (a) the continuous cleaning and chemical activation of the surfaces of nanoscale Fe/Pd bimetallic nanoparticles by the combined chemical and physical effects of acoustic cavitation; and (b) the accelerated mass transport rates of target POPs to the surfaces of the Fe/Pd nanoparticles...
October 26, 2018: Water Environment Research: a Research Publication of the Water Environment Federation
Summi Dutta, Manish Kumar, Kunal Mukhopadhyay
Obtaining high quality RNA in good quantities is often a requirement for plant-pathogen interaction studies, so it becomes very essential that a highly efficient method should be deployed to isolate RNA from minute quantities of fungal spores. The methods available to date, either require a high quantity of spores or the use of expensive chemicals. The protocol discussed here for RNA isolation from Puccinia triticina pathotype 77-5 urediniospores utilizes TRI Reagent as extraction buffer that is widely used for RNA isolation from plant tissues...
January 21, 2019: Journal of Microbiological Methods
Fahad Alkhudhairy, Mustafa Naseem, Zeeshan H Ahmad, Abrar N Alnooh, Fahim Vohra
AIM: The aim of the present study was to evaluate shear bond strength (SBS) of LDC and resin composite in combination with phototherapy and different ceramic surface treatments. MATERIALS AND METHOD: Forty Lithium Disilicate Glass Ceramic (LDC) disks measuring (4 × 4 x 9 mm) were fabricated. The specimens were randomly divided into four groups (n = 10 each) according to the surface treatment. Group 1 H F + Silane (Control); Group 2 HF + Ultrasonic bath + Silane; Group 3 SECP (Self etch ceramic primer) and Group 4 phototherapy (Er, Cr: YSGG) + Silane...
January 12, 2019: Photodiagnosis and Photodynamic Therapy
Yingyi Zhang, Liang Li, Xuqin Xing, Zhengzheng Zhou, Ande Ma
A chiral separation method was developed for the analysis of methamphetamine (METH) and amphetamine (AM) in hair by high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS). The samples were extracted using methanol by ultrasonication in a high-temperature water bath. The chromatographic separation was performed on a SUPELCO Astec CHIROIOTIC® V2 column with the mobile phase of methanol-20 mmol/L ammonium acetate aqueous solution containing 0.1% (v/v) formic acid (99:1, v/v). All the compounds showed linear relationship in the range of 15-300 ng/mg ( r >0...
December 8, 2018: Se Pu, Chinese Journal of Chromatography
Tatsuya Yamashita, Keita Ando
A number of acoustic and fluid-dynamic phenomena appear in ultrasonic cleaning baths and contribute to physical cleaning of immersed surfaces. Propagation and repeated reflection of ultrasound within cleaning baths build standing-wave-like acoustic fields; when an ultrasound intensity gradient appears in the acoustic fields, it can in principle induce steady streaming flow. When the ultrasound intensity is sufficiently large, cavitation occurs and oscillating cavitation bubbles are either trapped in the acoustic fields or advected in the flow...
December 5, 2018: Ultrasonics Sonochemistry
Merve Çakırbay Tanış, Canan Akay, Murat Şen
OBJECTIVE: To evaluate the shear bond strength of resin cements to zirconia treated with the selective infiltration etching technique (SIE). MATERIALS AND METHODS: Forty-seven zirconia specimens were prepared with a sequence of silicon carbide abrasives, cleaned in an ultrasonic bath. Three samples were separated for atomic force microscope (AFM) analyses (one nontreated for control, one sandblasted, and one selective infiltration etched) and the remaining were randomly distributed in four groups, according to the combination of surface treatment and resin cement: group 1a (sandblasting + Variolink II), group 1b (sandblasting + Panavia SA Plus), group 2a (SIE + Variolink II), group 2b (SIE + Panavia SA Plus)...
November 22, 2018: Journal of Esthetic and Restorative Dentistry
Hyun Soo Kim, Seung Ho Lee, Xun Sun, Joon Yong Yoon
In the present work, the performance of a waste-activated sludge (WAS) treatment using a novel rotational hydrodynamic cavitation generator (RHCG) was investigated. To verify the performance, a comparison with an ultrasonic device was conducted in four experimental cases at the same specific energy input using three assessment factors. The RHCG consisted of a rotor and three covers with inserted dimples. Ultrasonication was performed using a general ultrasonic bath. The experimental parameters were established to calculate the specific energy input of the sludge passing through the RHCG for a total of 5, 10, 15, and 20 times...
December 4, 2018: Ultrasonics Sonochemistry
Alberto Claudio Miano, Meliza Lindsay Rojas, Pedro Esteves Duarte Augusto
This work aimed to demonstrate the internal and external structural changes in potato cylinders caused by different times of ultrasound pretreatment. In addition, the structural changes were associated with the viscoelasticity and mass transfer. For which, potato cylinders were immersed in distilled water and pretreated with and without ultrasound (ultrasonic bath of 91 W/L and 25 kHz) up to 120 min. Then, the microstructure was evaluated by stereoscopic observation as a direct method, and by viscoelasticity and mass transfer evaluation (pigment diffusion and drying) as indirect methods...
November 15, 2018: Ultrasonics Sonochemistry
Manz Andrea Stefanie, Attin Thomas, Sener Beatrice, Sahrmann Philipp
BACKGROUND: Dentine hypersensitivity is especially frequent in patients with pronounced periodontal attachment loss. Aim of the treatment is an obstruction of the dentine tubules in order to inhibit liquid or osmotic motion, which is considered as trigger for pain sensations. Novel approaches aim for obstruction by calcium phosphate compounds in order to rely on biocompatible compounds. It was the aim of the study to optically investigate the morphology and to assess the fluid permeability of treated dentine surfaces...
November 21, 2018: BMC Oral Health
Marina Creydt, Maike Arndt, Daria Hudzik, Markus Fischer
The extraction of metabolites turns out to be one of the most important key factors for nontargeted metabolomics approaches as this step can significantly affect the informative value of the successive measurements. Compared to metabolomics experiments of various matrices of bacterial or mammalian origins, there are only few studies, which focus on different extraction methods for plant metabolomics analyses. In this study, various solvent extraction compositions were compared and assessed by using an UPLC-ESI-QTOF-MS strategy...
November 9, 2018: Journal of Agricultural and Food Chemistry
Karolina M Wójciak, Dariusz M Stasiak, Karolina Ferysiuk, Elżbieta Solska
Organic beef was dry fermented in sea salt (C) or acid whey (W). In order to test the effect of ultrasound, the W sample was subjected to sonication (U10) in an ultrasonic bath (10 min, 40 kHz, 480 W). The effect of sonication on fatty acid (FA) composition (%) and atherogenicity, thrombogenicity, hypocholesterolemic/hypercholesterolemic, peroxidistability indexes (AI, TI, h/H, and PI, respectively), thiobarbituric acid reactive substances (TBARS) value, and color parameters were investigated during ripening period (31, 62, and 93 days)...
February 2019: Meat Science
Amirtaher Mirmortazavi, Hamidreza Rajati Haghi, Abdolmajid Fata, Hossein Zarrinfar, Hossein Bagheri, Amirhossein Mehranfard
Background and Purpose: Candida -associated denture stomatitis is one of the most common forms of oral candidiasis among denture wearers. Regarding this, the aim of the present study was to evaluate the antifungal effects of home-generated ozonated water on the adhesion of the C.  albicans attached to the surface of the denture base acrylic resins. Materials and Methods: For the purpose of the study, different concentrations of C. albicans were added to the tubes containing acrylic resin blocks, and then incubated for 2 h at 35°C...
June 2018: Advances in Medical Mycology (Iran)
Marta Gładysz, Małgorzata Król, Patryk Własiuk, Michał Piwowar, Grzegorz Zadora, Paweł Kościelniak
In this study, the effective and robust semi-destructive ultrasound assisted extraction (UAE) method followed by gas chromatography coupled to mass spectrometry (GC-MS) method used for analysis of red lipsticks samples was developed, optimized and evaluated. 43 red lipsticks of a very similar hue representing of 21 different manufacturers were investigated. The red lipsticks (approximately 0.05 mg) were applied on a disc (of ø 2 mm) placed on specially designed stand printed with a 3D printer. In order to optimize the main factors affecting extraction process, Doehlert experimental design with response surface methodology was applied...
November 23, 2018: Journal of Chromatography. A
Maria V Chandra-Hioe, Jenny Elvira, Jayashree Arcot
Lutein is a xanthophyll, a bioactive phytochemical that presents itself as colourful pigments in plants. Australian sweet lupin flour has been incorporated as a food ingredient in wheat bread and pasta to improve their sensory property and nutritional quality. However, the amount of lutein in lupin flour has not yet been determined. This is the first study to quantify naturally occurring lutein in Australian sweet lupin flour after the extraction efficiency was optimised. Several organic solvents (acetone, isopropyl alcohol, ethyl acetate and hexane), the use of an ultrasonic bath or a probe, the need for saponification and addition of ascorbic acid (served as antioxidant) were tested to compare the extraction yield...
October 10, 2018: Plant Foods for Human Nutrition
Adriana Fernández Campos, Ricardo J Cassella
The present work reports the development of ion chromatography conductivity based detection analytical method for the determination of some inorganic anions (chloride, sulfate and phosphate) in vegetable oils. The analytes were extracted from samples prior to injection into the chromatographic system, employing a simple two-step procedure. In the first step, 4.5 g of the sample was vigorously mixed with 15 mL of deionized water and then mixed for 15 min on a horizontal roller. Afterwards, the mixture was sonicated for 15 min in an ultrasonic bath...
October 2018: Journal of Food Science and Technology
Nicolas Van Elsué, Cleo L Crunelle, Cor A Verbrugge, Kim van Baarle, Anaïs Rodrigues, Hugo Neels, Michel Yegles
Gamma-hydroxybutyric acid (GHB) is a short-chain fatty acid used recreationally as a drug of abuse due its strong suppressive effect on the central nervous system. The detection window of GHB in blood and urine is very narrow (t1/2=30min) but can be substantially prolonged using alternative matrices such as hair. We here present a newly developed and limited validated method with a solid phase extraction (SPE) using GC-MS/MS to determine concentrations of GHB in hair samples. The soft extraction technique (water and 90min ultrasonic bath) preserves GHB with a high yield and clean extracts...
October 2018: Forensic Science International
Karolina Celejewska, Monika Mieszczakowska-Frąc, Dorota Konopacka, Tomasz Krupa
The aim of the study was to characterize physical and chemical composition of four highbush blueberry cultivars ("Bluecrop," "Late Blue," "Chandler," and "Elliot"). The assessment of ultrasound influence at the osmotic dehydration step and during hybrid drying on mass transfer and the nutritional quality of all cultivars was also done. The experiment consisted of two stages: (1) initial: optimization of osmotic dehydration procedure, (2) the main experiment including all stages of dried blueberry production (pretreatment, osmotic dehydration chosen in the initial stage, and drying)...
September 2018: Journal of Food Science
A I Argüelles-Pesqueira, N M Diéguez-Armenta, A K Bobadilla-Valencia, S K Nataraj, A Rosas-Durazo, R Esquivel, M E Alvarez-Ramos, Roberto Escudero, P Guerrero-German, J A Lucero-Acuña, P Zavala-Rivera
Here we demonstrate a simple method for the organic sonosynthesis of stable Iron Carbide@Iron Oxide core-shell nanoparticles (ICIONPs) stabilized by oleic acid surface modification. This robust synthesis route is based on the sonochemistry reaction of organometallic precursor like Fe(CO)5 in octanol using low intensity ultrasonic bath. As obtained, nanoparticles diameter sizes were measured around 6.38 nm ± 1.34 with a hydrodynamic diameter around 25 nm and an estimated polydispersity of 0.27. Core-Shell structure of nanoparticles was confirmed using HR-TEM and XPS characterization tools in which a core made up of iron carbide (Fe3 C) and a shell of magnetite (γ-Fe2 O3 ) was found...
December 2018: Ultrasonics Sonochemistry
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