Qinggang Hao, Rui Dong, Weiyu Bai, Dong Chang, Xinyi Yao, Yingru Zhang, Huangying Xu, Huiyan Li, Xiang Kui, Feng Wang, Yan Wang, Chengqin Wang, Yujie Lei, Yan Chen, Junling Shen, Lei Sang, Yan Bai, Jianwei Sun
Tumor metastasis, responsible for approximately 90% of cancer-associated mortality, remains poorly understood. Here in this study, we employed a melanoma lung metastasis model to screen for metastasis-related genes. By sequential tail vein injection of mouse melanoma B16F10 cells and the subsequently derived cells from lung metastasis into BALB/c mice, we successfully obtained highly metastatic B16F15 cells after five rounds of in vivo screening. RNA-sequencing analysis of B16F15 and B16F10 cells revealed a number of differentially expressed genes, some of these genes have previously been associated with tumor metastasis while others are novel discoveries...
February 29, 2024: Biophysics Reports