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Indian Journal of Clinical Biochemistry: IJCB

Deepak Kumar, Vandana Singh, Sakshi Behal, Kunal Maniar, Sheemona Chawdhary, Rajasri Bhattacharyya, Dibyajyoti Banerjee
No abstract text is available yet for this article.
January 2019: Indian Journal of Clinical Biochemistry: IJCB
Elham Ghadami, Ahmad Tamaddoni, Sadegh Sedaghat, Reza Tabaripour, Hadis Pourreza Baboli, Haleh Akhavan-Niaki
α-Thalassemia is one of the most common monogenic diseases worldwide. The combination of alpha-chain variants with thalassemia mutations may lead to clinical and hematological characteristics, which is of importance for genetic counseling. The present study describes for the first time a rare α1-globin nonsense mutation, codon 99 ( HBA1 : c.298A>T) associated with a α2 -chain variant Hb Fontainebleau ( HBA2 : c.64G>C) in a family from northern Iran. The case is a 23-year-old man with hypochromic microcytic anemia that requested for prenatal diagnosis...
January 2019: Indian Journal of Clinical Biochemistry: IJCB
Anbalagan Anithasri, Palghat Harihara Ananthanarayanan, P Veena
Omentin-1 and prostate specific antigen (PSA) are known to be markers of insulin resistance and hyperandrogenism respectively in polycystic ovary syndrome (PCOS). This study aimed to assess the changes in serum omentin-1 and PSA levels in PCOS patients while on treatment. Serum omentin-1, PSA, BMI and Ferriman gallwey score (FG score) were measured in 80 women with PCOS. The subjects were treated depending on their presenting complaints. The parameters were analysed at baseline and after 3 months of treatment viz...
January 2019: Indian Journal of Clinical Biochemistry: IJCB
Noorhan K Shafeeq
In the study, it is aimed to research the likely role of visfatin in hyperthyroid and subclinical hyperthyroid status related to dislipidemia, after determining the visfatin and PON-1 levels thirty healthy individuals were enrolled in this study as control group and thirty hyperthyroidism patient's with dyslipidemia they were treated with. The subjects aged ranged (30-50) years and with BMI (25-29) kg/m2 . Serum was used in determination of lipid profile total cholesterol (TC), triglyceride (TG), high density lipoprotein (HDL), low density lipoprotein (LDL), very low density lipoprotein (VLDL), thyroid threonine (T3) and thyroxin (T4), thyroid stimulating hormone (TSH), visfatin and paraoxonase-1 (PON-1)...
January 2019: Indian Journal of Clinical Biochemistry: IJCB
Elif Azarsiz, Gamze Eman, Sanem Eren Akarcan, Ezgi Ulusoy Severcan, Neslihan Karaca, Guzide Aksu, Necil Kutukculer
Anti-beta-2-glycoprotein I antibodies (anti-β2GPI) which are the main antiphospholipid antibodies that characterize the autoimmune "antiphospholipid syndrome" are pathogenic and are contributing to thrombosis. We aimed to evaluate the presence and the diagnostic importance of these antibodies in children with different rheumatologic diseases with or without thrombosis risk. A total of 100 children with different rheumatologic diseases evaluated retrospectively. The mean anti-β2GPI IgG ( p  = 0...
January 2019: Indian Journal of Clinical Biochemistry: IJCB
Srinivas Narasimhachar Chakravarthy, Satish Ramanathan, Smitha S, Thirumalai Nallathambi, Micheal S
Verification of analytical performance of measurands becomes an essential requirement for the laboratories before proceeding to patients' samples testing. In our study we have verified the performance of HbA1C Immunoturbidimetric assay (VITROS 5600) against manufacturers' claims using CLSI EP15A3 Guidelines. We performed our study using two concentrations of Quality Control from Bio-Rad (Level 1 and Level 2). A precision verification study was carried out using five replicates of QC per day for five days following which imprecision estimates in form of Within Run (Repeatability) %CV and Within Lab %CV were calculated and compared against manufacturer's claims...
January 2019: Indian Journal of Clinical Biochemistry: IJCB
Ozlem Dogan, Muhittin A Serdar, Koza Murat, Cigdem Sonmez, Emre İspir, Mustafa Serteser, Ibrahim Unsal
Analysis of porphyrins and 5-aminolevulinic acid (ALA), porphobilinogen (PBG) in physiological liquids is required for diagnosis and follow-up of porphyrias. High performance liquid chromatography (HPLC) and liquid chromatography tandem mass spectrometry (LC-MS) methods with higher specificity and sensitivity have been developed. The major disadvantage of those methods is that they require longer extraction times due to their matrix effects. The present study suggests a simple, fast, sensitive, and specific assay for determination of Coproporphyrin, 5-carboxylporphyrin, 6-carboxylporphyrin, 7-carboxylporphyrin, Uroporphyrin I and ALA, PBG in urine sample by direct injection without sample pre-treatment using LC-MS...
January 2019: Indian Journal of Clinical Biochemistry: IJCB
Eldho Kuriakose, Sumithra Narayanan Unni Cheppayil, Subhakumari Kuzhikandathil Narayanan, Anu Vasudevan
Demonstration of monoclonal immunoglobulin molecule in serum forms the mainstay in the diagnosis of monoclonal gammopathies. The major tests that help in this regard are serum protein electrophoresis (SPEP), serum immunofixation electrophoresis (sIFE) and serum free light chain assay (sFLC). Our objectives were to study the accuracy of sFLC and sIFE in the diagnosis of monoclonal gammopathies and also to study the role of combination of SPEP + sIFE + sFLC in the diagnosis of the same. 46 patients who attended the hemato-oncology clinic with signs and symptoms suggestive of monoclonal gammopathy were enrolled in this study...
January 2019: Indian Journal of Clinical Biochemistry: IJCB
Young-Ok Son, Jung-Min Hwang, Ki-Choon Choi, Jeong-Chae Lee
The aims of this study were to explore whether a phenolic acid and flavonoid fraction (named PAFF) isolated from Lolium multiflorum Lam. protects against d-galactosamine (GalN)-induced liver damages in mice and to investigate the associated mechanisms. ICR mice received oral administration with various concentrations (50, 100, and 200 mg/kg body weight) of PAFF once per 2 days for seven times before intraperitoneal injection with 800 mg/kg GalN. After a day of GalN challenge, blood and tissue samples were analyzed by biochemical, histopathological, real time RT-PCR, and Western blot methods...
January 2019: Indian Journal of Clinical Biochemistry: IJCB
Joseph Dian Bondu, M S Seshadri, R Selvakumar, Jude Joseph Fleming
We investigated the combined effect of fluoride exposure and Vitamin D deficiency in causing bone damage as a precursor to development of Fluorotoxic Metabolic Bone Disease. Thirty-six male Sprague-Dawley rats were divided into 6 groups of six; 3 groups received a Vitamin D deficient diet whereas the other 3 received a Vitamin D adequate diet. Serum total 25-hydroxyvitamin D (25OHD), calcium, phosphorus, creatinine, Alkaline phosphatase (ALP), albumin, Parathyroid hormone (PTH), Osteocalcin and C terminal telopeptide (CTx) were measured following exposure to varying levels of fluoride in drinking water (< 1...
January 2019: Indian Journal of Clinical Biochemistry: IJCB
S Chandrashekara, Sachin Vithalrao Dhote, K R Anupama
Metabolic parameters like uric acid, lipids and homocysteine are influenced by immunopathological mechanisms underlying the autoimmune disease processes. The current study examined the differences in these parameters and the correlation between inflammatory and metabolic variables in rheumatoid arthritis (RA) and systemic lupus erythematosus (SLE) patients. The cross-sectional prospective study included 24 treatment-naïve patients with moderate to severe diseases-15 subjects had RA and 9 had SLE. Atherogenic index of plasma (AIP) was used to assess the cardiovascular risk of the patients...
January 2019: Indian Journal of Clinical Biochemistry: IJCB
Archana Verma, Vibha Singh, Praveen Kumar Jaiswal, Rama D Mittal
GEMIN4 is a member of the GEMIN gene family which is involved in multiple pathologies including cancer. It is located on Chr17p13.3, the most notorious chromosome and a hotspot for various carcinomas. We therefore intend to find genetic variants of GEMIN4 gene associated with renal cell carcinoma risk (RCC). This study comprised 100 patients and 225 controls. Genotyping of GEMIN4 gene variants was done using Taqman® assay. The association of GEMIN4 variants and risk prediction of RCC was done by statistical analysis...
January 2019: Indian Journal of Clinical Biochemistry: IJCB
Shailendra Dwivedi, Purvi Purohit, Radhieka Misra, Puneet Pareek, Jeewan Ram Vishnoi, Sanjeev Misra, Praveen Sharma
Inadequate methods may cause substantial loss not only in the quantity but also in quality of the product. This study aimed to determine the best method for making single cell suspension for isolation of RNA and flow cytometer analysis from cancer tissue. We compared two methods of tissue disruption used during RNA isolation and flow cytometer analysis. Mechanical tissue disruption method and enzymatic tissue digestion method are commonly used for making single cell suspension before RNA isolation and flow cytometer analysis...
January 2019: Indian Journal of Clinical Biochemistry: IJCB
Meena M Abdulhussain, Najat A Hasan, Alaa G Hussain
The changes in the translational repression and variation in mRNA degradation induced by micro RNA are important aspects of tumorigenesis. The association of microRNA-21 with clinicopathologic features and expression of programed cell death 4 (PDCD4) in Iraqi female's with breast tumors has not been studied. MicroRNAs were extracted from a set of 60 breast tumor tissues and blood samples of females with breast cancer and benign breast lesions obtained after breast-reductive surgery, and only blood samples from 30 normal volunteers...
January 2019: Indian Journal of Clinical Biochemistry: IJCB
Rama Devi Mittal
Multitude of gene-altering capabilities in combination with ease of design and low cost have all led to the adoption of the sophisticated and yet simple gene editing system that are clustered regularly interspaced short palindromic repeats (CRISPR)-associated protein 9 (CRISPR). The CRISPR/Cas9 system holds promise for the correction of deleterious mutations by taking advantage of the homology directed repair pathway and by supplying a correction template to the affected patient's cells. CRISPR is a tool that allows researchers to edit genes very precisely, easily and quickly...
January 2019: Indian Journal of Clinical Biochemistry: IJCB
Shailendra Dwivedi, Purvi Purohit, Radhieka Misra, Malavika Lingeswaran, Jeewan Ram Vishnoi, Puneet Pareek, Sanjeev Misra, Praveen Sharma
Breast cancer is recognized for its different clinical behaviors and patient outcomes, regardless of common histopathological features at diagnosis. The heterogeneity and dynamics of breast cancer undergoing clonal evolution produces cells with distinct degrees of drug resistance and metastatic potential. Presently, single cell analysis have made outstanding advancements, overshadowing the hurdles of heterogeneity linked with vast populations. The speedy progression in sequencing analysis now allow unbiased, high-output and high-resolution elucidation of the heterogeneity from individual cell within a population...
January 2019: Indian Journal of Clinical Biochemistry: IJCB
Shailendra Dwivedi, Purvi Purohit, Praveen Sharma
No abstract text is available yet for this article.
January 2019: Indian Journal of Clinical Biochemistry: IJCB
P Purohit, D Khera, K Singh
Hypocalcemia is a laboratory and clinical abnormality that is observed especially in neonates and paediatric patients. Laboratory hypocalcaemia is often asymptomatic but it can manifest as central nervous system irritability, paraesthesia, tetany (i.e. contraction of hands, arms, feet, larynx, bronchioles), seizures, and even psychiatric changes in children. Cardiac function may also be impaired because of poor muscle contractility. We report a unique case of an eleven year old male child who presented with chronic kidney disease associated with severe hypocalcemia, tonic-clonic seizures, hypovitaminosis D but normal electroencephalogram and electrocardiography...
October 2018: Indian Journal of Clinical Biochemistry: IJCB
Sheemona Chowdhary, Dibyajyoti Banerjee
No abstract text is available yet for this article.
October 2018: Indian Journal of Clinical Biochemistry: IJCB
Bineeta Kashyap, Nisha Goyal, G K Das, N P Singh, I R Kaur
TB as the cause of uveitis varies from 0.5 to 10.5%; low sensitivity of confirmatory laboratory investigations and inconsistency of diagnostic criteria leads to paucity of data. Diagnosis requires a high level of suspicion and is often presumptive based on indirect evidences. Interferon gamma, Interleukin-2 and Neopterin are key biomarkers in immuno-regulation of Mycobacterium tuberculosis infection. The relative shift from Interleukin-2 towards Interferon gamma (Interferon gamma/Interleukin-2) is more discriminatory for active tuberculosis...
October 2018: Indian Journal of Clinical Biochemistry: IJCB
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