Adeliia Giliazeva, Yaw Akosah, Jonas Noack, Ayslu Mardanova
The intestinal and respiratory tracts of healthy individuals serve as habitats for a diverse array of microorganisms, among which Klebsiella oxytoca holds significance as a causative agent in numerous community- and hospital-acquired infections, often manifesting in polymicrobial contexts. In specific circumstances, K. oxytoca, alongside other constituents of the gut microbiota, undergoes translocation to distinct physiological niches. In these new environments, it engages in close interactions with other microbial community members...
April 8, 2024: Microbial Pathogenesis
Chalbia Mansour, Fadia Ben Taheur, Sihem Safta Skhiri, Mourad Jridi, Dalila Saidane Mosbahi, Nacim Zouari
This study aimed to investigate the impact of incorporating kefir into the diet on biometric parameters, as well as the immune and antioxidant responses of the carpet shell clam (Ruditapes decussatus) after an experimental infection by Vibrio alginolyticus. Clams were divided into a control group and a treated group. The control group was fed on spirulina (Arthrospira platensis) alone. While, the treated group was fed on spirulina supplemented with 10% dried kefir. After 21 days, clams were immersed in a suspension of V...
April 6, 2024: Microbial Pathogenesis
Azhagu Madhavan Sivalingam, Arjun Pandian
The plant Erythrina indica comes under Fabaceae family, mainly used for used in traditional medicine as nervine sedative, antiepileptic, antiasthmatic, collyrium in opthalmia, antiseptic. Current study focused synthesize of silver nanoparticles (AgNPs) by E. indica leaf ethanol extract. The green-synthesized AgNPs underwent characterization using multiple analytical techniques, including UV-visible, FTIR, DLS, SEM, TEM, XRD, and EDX, and estimation of their antioxidant activity and antimicrobial activity. Phytochemical analysis identified alkaloids, tannins, saponins, flavonoids, and phenols as secondary metabolites...
April 3, 2024: Microbial Pathogenesis
Gaojie Song, Cuiling Zhang, Xiaoyang Yu, Jiaqi Li, Qinlei Fan, Fei Liu, Qinghao He, Chao Shang, Xiao Li
Autophagy plays an important role in the lifecycle of viruses. However, there is currently a lack of systematic research on the relationship between Infectious Bronchitis Virus (IBV) and autophagy. This study aims to investigate the impact of IBV on autophagy and the role of autophagy in viral replication. We observed that IBV infection increased the expression of microtubule-associated protein 1 light chain 3, a marker of autophagy, decreased the expression of sequestosome 1, and led to elevated intracellular LC3 puncta levels...
April 2, 2024: Microbial Pathogenesis
Min Xu, Jing Zeng
We seek to investigate the multifaceted factors influencing secondary infections in patients with multidrug-resistant Gram-negative bacteria (MDR-GNB) colonization or infection post-hospitalization. A total of 100 patients with MDR-GNB colonization or infection were retrospectively reviewed, encompassing those admitted to both the general ward and intensive care unit of our hospital from August 2021 to December 2022. Patients were categorized into the control group (non-nosocomial infection, n = 56) and the observation group (nosocomial infection, n = 44) based on the occurrence of nosocomial infection during hospitalization...
April 1, 2024: Microbial Pathogenesis
Nora DeLira-Bustillos, Uriel A Angulo-Zamudio, Nidia Leon-Sicairos, Hector Flores-Villaseñor, Jorge Velazquez-Roman, Gabriela Tapia-Pastrana, Francisco A Martínez-Villa, Rafael Velázquez-Cruz, Jorge Salmerón, Samuel Canizales-Quinteros, Adrian Canizalez-Roman
This study aimed to determine the prevalence of cyclomodulins (cdt, cnf, pks and cif) in Escherichia coli (E. coli) isolated from clinical and environmental samples, the presence of supplementary virulence genes (SVG), antibiotic resistance, and in vitro cytotoxicity. 413 E. coli were isolated from clinical (stool from obese subjects, normal weight subjects, children with diarrhea, and children without diarrhea; and urine from pregnant and non-pregnant women with urinary tract infections) and environmental (water and different foods) samples...
March 29, 2024: Microbial Pathogenesis
Alafate Ayibieke, Takeaki Wajima, Shigeyuki Kano, Nabendu Sekhar Chatterjee, Takashi Hamabata
Enterotoxigenic Escherichia coli (ETEC) is one of the main causes of diarrhea in children and travelers in low-income regions. The virulence of ETEC is attributed to its heat-labile and heat-stable enterotoxins, as well as its colonization factors (CFs). CFs are essential for ETEC adherence to the intestinal epithelium. However, its invasive capability remains unelucidated. In this study, we demonstrated that the CS6-positive ETEC strain 4266 can invade mammalian epithelial cells. The invasive capability was reduced in the 4266 ΔCS6 mutant but reintroduction of CS6 into this mutant restored the invasiveness...
March 29, 2024: Microbial Pathogenesis
Chengxin Zhang, Fang He, Nianfeng Li, Wei Du, Jianxin Wen, Xiaoyan Wu, Jianli Shi, Chen Li, Chang Liu, Shaojian Xu, Hong Han, Nataliia Hrabchenko, Xianjie Han, Jun Li
Porcine circovirus type 2 (PCV2) is a globally prevalent infectious pathogen affecting swine, with its capsid protein (Cap) being the sole structural protein critical for vaccine development. Prior research has demonstrated that PCV2 Cap proteins produced in Escherichia coli (E. coli) can form virus-like particles (VLPs) in vitro, and nuclear localization signal peptides (NLS) play a pivotal role in stabilizing PCV2 VLPs. Recently, PCV2d has emerged as an important strain within the PCV2 epidemic. In this study, we systematically optimized the PCV2d Cap protein and successfully produced intact PCV2d VLPs containing NLS using E...
March 29, 2024: Microbial Pathogenesis
Cuiyu Ba, Chaonan Tian, Xueying Bo, Chang Xu, Yi Zhang, Xiangyu Sun, Yuchen Nan, Chunyan Wu
Interferon-stimulated gene product 15 (ISG15) can be conjugated to substrates through ISGylation. Currently, the E3 ligase for porcine ISGylation remains unclear. Here, we identified porcine HERC5 and HERC6 (pHERC5/6) as ISGylation E3 ligase whereas pHERC6 acts as major E3 ligase by reconstitution of porcine ISGylation system in HEK-293T cell via transfecting E1, E2, E3 and porcine ISG15(pISG15) genes. Meanwhile, our data demonstrated that co-transfection of pISG15 and pHERC5/6 was sufficient to confer ISGylation, suggesting E1 and E2 of ISGylation are interchangeable between human and porcine...
March 28, 2024: Microbial Pathogenesis
Jiying Xiao, Lin Su, Xiya Chen, Shumin Huang, Mingming Zhou, Zhimin Chen
With the widespread introduction of the Hib conjugate vaccine, Nontypeable Haemophilus influenzae (NTHi) has emerged as the predominant strain globally. NTHi presents a significant challenge as a causative agent of chronic clinical infections due to its high rates of drug resistance and biofilm formation. While current research on NTHi biofilms in children has primarily focused on upper respiratory diseases, investigations into lower respiratory sources remain limited. In this study, we collected 54 clinical strains of lower respiratory tract origin from children...
March 26, 2024: Microbial Pathogenesis
Hongxia Niu, Chunxiang Bai, Bingdong Zhu, Ying Zhang
The formation of long-lived T-cell memory is a critical goal of vaccines against intracellular pathogens like Mycobacterium tuberculosis (M. tuberculosis). In this study, to access the adjuvant effect of rapamycin on tuberculosis subunit vaccine, we treated mice with rapamycin during the course of vaccination and then monitored the vaccine-specific long-term memory T cell recall responses and protective ability against mycobacterial organisms. Compared with the mice that received vaccine alone, rapamycin treatment enhanced the vaccine induced long-term IFN-γ and IL-2 recall responses, promoted the development of TCM (central memory) like cells and improved the long-term proliferative ability of lymphocytes...
March 26, 2024: Microbial Pathogenesis
Yuxin Sun, Hui-Zhu Nan, Chao Zhang, Xin Yang, Yan Zhao, Gang Feng, Lei Ma
Blastocystis sp. is a protozoan parasite that infects the intestines of humans and animals, causing chronic diseases such as skin rashes, abdominal pain, and irritable bowel syndrome. A survey was conducted to determine the prevalence and genetic diversity of Blastocystis sp. infection in cattle, in Hebei Province, China. 2746 cattle fecal samples were collected from 11 cities in Hebei Province and analyzed using polymerase chain reaction targeting the Blastocystis sp. barcoding gene. MEGA, PhyloSuite, and PopART were used to analyze the subtype, sequence signature, pairwise genetic distance, and genetic diversity indices...
March 22, 2024: Microbial Pathogenesis
Asif A Malik, Nisar A Dangroo, Parminder Kaur, Shobit Attery, Manzoor A Rather, Abrar Khan, Tabassum Ara, Hemraj Nandanwar
Overexpression of the efflux pump is a predominant mechanism by which bacteria show antimicrobial resistance (AMR) and leads to the global emergence of multidrug resistance (MDR). In this work, the inhibitory potential of library of dihydronapthyl scaffold-based imidazole derivatives having structural resemblances with some known efflux pump inhibitors (EPI) were designed, synthesized and evaluated against efflux pump inhibitor against overexpressing bacterial strains to study the synergistic effect of compounds and antibiotics...
March 21, 2024: Microbial Pathogenesis
Xin Liu, Changjiang Wang, Yu Bai, Weichen Wang, Yuchen Han, Shu Cai, Jiajia An, Guanggang Qu
In this study, we successfully developed a nanobody-based double antibody sandwich ELISA kit for the detection of clinical serum C-reactive protein (CRP) by using two novel CRP specific nanobodies. The developed method exhibited a linear detection range of approximately 6-200 ng/mL, with a detection limit of 1 ng/mL. Furthermore, the method demonstrated excellent specificity, as there was no cross-reactivity with interfering substances such as total bilirubin and hemoglobin and so on. To assess reproducibility, independent measurements of the samples were conducted under experimental conditions, resulting in intra- and inter-batch coefficients of variation below 10% and a recovery rate of 93%-102%...
March 21, 2024: Microbial Pathogenesis
Ana Luíza A R Martin, Raimundo Luiz Silva Pereira, Janaína Esmeraldo Rocha, Pablo A M Farias, Thiago S Freitas, Francisco Rodrigo de Lemos Caldas, Fernando G Figueredo, Nadghia Figueiredo Leite Sampaio, Cícera Datiane de Morais Oliveira-Tintino, Saulo Relison Tintino, Gabriel Costa A da Hora, Maria Carolina Pacheco Lima, Irwin Rose A de Menezes, Diogo T Carvalho, Henrique D M Coutinho, Marta M F Fonteles
The occurrence of bacterial resistance has been increasing, compromising the treatment of various infections. The high virulence of Staphylococcus aureus allows for the maintenance of the infectious process, causing many deaths and hospitalizations. The MepA and NorA efflux pumps are transporter proteins responsible for expelling antimicrobial agents such as fluoroquinolones from the bacterial cell. Coumarins are phenolic compounds that have been studied for their diverse biological actions, including against bacteria...
March 20, 2024: Microbial Pathogenesis
Yucheng Huang, Qian Zhu, Yongqiang Wang, Kui Zhu
Rotavirus infections in suckling and weaning piglets cause severe dehydration and death, resulting in significant economic losses in the pig breeding industry. With the continuous emergence of porcine rotavirus (PoRV) variants and poor vaccine cross-protection among various genotypes, there is an urgent need to develop alternative strategies such as seeking effective antiviral products from nature, microbial metabolites and virus-host protein interaction. Sialidases play a crucial role in various physiopathological processes and offer a promising target for developing antivirus drugs...
March 18, 2024: Microbial Pathogenesis
Moumita Malik, Sharmistha Das, Poulomi Chakraborty, Payel Paul, Ritwik Roy, Anirban Das Gupta, Sarita Sarkar, Sudipta Chatterjee, Alakesh Maity, Monikankana Dasgupta, Ranojit Kumar Sarker, Prosun Tribedi
Pseudomonas aeruginosa is widely associated with biofilm-mediated antibiotic resistant chronic and acute infections which constitute a persistent healthcare challenges. Addressing this threat requires exploration of novel therapeutic strategies involving the combination of natural compounds and conventional antibiotics. Hence, our study has focused on two compounds; cuminaldehyde and ciprofloxacin, which were strategically combined to target the biofilm challenge of P. aeruginosa. The minimum inhibitory concentration (MIC) of cuminaldehyde and ciprofloxacin was found to be 400 μg/mL and 0...
March 14, 2024: Microbial Pathogenesis
Priyankar Dey
This review examines the complex connection between commensal microbiota and the development of opportunistic infections. Several underlying conditions, such as metabolic diseases, malignancies, and weakened immune systems, increase the vulnerability of patients to opportunistic infections. The increasing antibiotic resistance adds significant complexity to the management of infectious diseases. Although commensals have long been considered beneficial, recent research contradicts this notion by uncovering chronic illnesses linked to atypical pathogens or commensal bacteria...
March 14, 2024: Microbial Pathogenesis
Jiaxin Chen, Run Cai, Lu Tang, Dan Wang, Ruiwei Lv, Changhong Guo
Root rot is a common disease, that severely affects the yield and quality of alfalfa. Biocontrol is widely used to control plant diseases caused by pathogenic fungi, however, biocontrol strains for alfalfa root rot are very limited. In this study, a Bacillus subtilis CG-6 strain with a significant biocontrol effect on alfalfa root rot was isolated. CG-6 secretes antibacterial enzymes and siderophore, phosphate solubilization and indoleacetic acid (IAA). The inhibition rate of strain CG-6 against Fusarium oxysporum was 87...
March 14, 2024: Microbial Pathogenesis
Yi-Han Ma, Yu-Di Sheng, Di Zhang, Jun-Tong Liu, Ye Tian, Hui Li, Xiao-Fei Li, Na Li, Peng Sun, Shahrood Ahmed Siddiqui, Wu-Wen Sun, Lei Zhang, Xiao-Feng Shan, Chun-Feng Wang, Ai-Dong Qian, Dong-Xing Zhang
Lactic acid bacteria (LAB) have been recognized as safe microorganism that improve micro-flora disturbances and enhance immune response. A well-know traditional herbal medicine, Acanthopanax senticosus (As) was extensively utilized in aquaculture to improve growth performance and disease resistance. Particularly, the septicemia, skin wound and gastroenteritis caused by Aeromonas hydrophila threaten the health of aquatic animals and human. However, the effects of probiotic fermented with A. senticosus product on the immune regulation and pathogen prevention in fish remain unclear...
March 14, 2024: Microbial Pathogenesis
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