journal
https://read.qxmd.com/read/38428469/dual-rpa-assay-for-rapid-detection-and-differentiation-of-e-granulosus-and-e-multilocularis
#21
JOURNAL ARTICLE
Shu Cai, Changjiang Wang, Fengrong Tian, Mengxiao Liu, Hongbin Yan, Zaiyan Xu, Guanggang Qu
Echinococcus granulosus (Eg) and Echinococcus multilocularis (Em) are the two most widely prevalent types of echinococcosis. Several diagnostic methods have been developed for detecting Eg and Em. However, some limitations, such as being time-consuming, needing expensive instruments, or exhibiting low sensitivity, make these methods unsuitable for on-site detection. In this study, a dual-RPA assay was established to detect and differentiate Eg and Em. The primer concentration ratio, reaction time, and reaction temperature of the dual-RPA were optimized...
February 28, 2024: Microbial Pathogenesis
https://read.qxmd.com/read/38423406/platycodon-grandiflorus-polysaccharide-inhibits-the-inflammatory-response-of-3d4-21%C3%A2-cells-infected-with-pcv2
#22
JOURNAL ARTICLE
Xiaocheng Guo, Ximan Zhao, Linjue Li, Menglin Jiang, Aiqin Zhou, Yifan Gao, Pimiao Zheng, Jianzhu Liu, Xiaona Zhao
Porcine circovirus type 2 (PCV2) infection cause multi-systemic inflammation in pigs. Platycodon grandiflorus polysaccharide (PGPSt ) has been reported to have the effects of immune regulation and disease resistance. Nevertheless, the role and mechanism of PGPSt in the inflammatory response of 3D4/21 cells induced by PCV2 infection remain unclear. The present study aims to investigate effects of PGPSt on inflammatory response and its possible underlying mechanisms in vitro models. Cells were treated with PCV2 for 36 h to construct a cell inflammation model...
February 27, 2024: Microbial Pathogenesis
https://read.qxmd.com/read/38423405/br-nanoconjugate-enhances-the-antibacterial-efficacy-of-nimboloide-against-flavobacterium-columnare-infection-in-labeo-rohita-a-nanoinformatics-approach
#23
JOURNAL ARTICLE
Sanjana Mishra, Prekshi Garg, Shilpi Srivastava, Prachi Srivastava
BACKGROUND: The bacterial pathogen, Flavobacterium columnare causes columnaris disease in Labeo rohita globally. Major effects of this bacterial infection include skin rashes and gill necrosis. Nimbolide, the key ingredient of the leaf extract of Azadirachta indica possesses anti-bacterial properties effective against many microorganisms. Nano-informatics plays a promising role in drug development and its delivery against infections caused by multi-drug-resistant bacteria. Currently, studies in the disciplines of dentistry, food safety, bacteriology, mycology, virology, and parasitology are being conducted to learn more about the wide anti-virulence activity of nimbolide...
February 27, 2024: Microbial Pathogenesis
https://read.qxmd.com/read/38423404/piperine-a-phytochemical-prevents-the-biofilm-city-of-methicillin-resistant-staphylococcus-aureus-a-biochemical-approach-to-understand-the-underlying-mechanism
#24
JOURNAL ARTICLE
Sharmistha Das, Moumita Malik, Debabrata Ghosh Dastidar, Ritwik Roy, Payel Paul, Sarita Sarkar, Poulomi Chakraborty, Alakesh Maity, Monikankana Dasgupta, Anirban Das Gupta, Sudipta Chatterjee, Ranojit Kumar Sarker, Debasish Maiti, Prosun Tribedi
Methicillin-resistant Staphylococcus aureus (MRSA), a drug-resistant human pathogen causes several nosocomial as well as community-acquired infections involving biofilm machinery. Hence, it has gained a wide interest within the scientific community to impede biofilm-induced MRSA-associated health complications. The current study focuses on the utilization of a natural bioactive compound called piperine to control the biofilm development of MRSA. Quantitative assessments like crystal violet, total protein recovery, and fluorescein-di-acetate (FDA) hydrolysis assays, demonstrated that piperine (8 and 16 μg/mL) could effectively compromise the biofilm formation of MRSA...
February 27, 2024: Microbial Pathogenesis
https://read.qxmd.com/read/38423403/antibacterial-activity-and-mechanism-of-cell-free-supernatants-of-lacticaseibacillus-paracasei-against-propionibacterium-acnes
#25
JOURNAL ARTICLE
Jiahui Xu, Xiaoyong Chen, Jiajia Song, Chen Wang, Weiping Xu, Han Tan, Huayi Suo
Propionibacterium acnes (P. acnes) is an anaerobic and gram-positive bacterium involved in the pathogenesis and inflammation of acne vulgaris. This study particularly focuses on the antimicrobial effect of Lacticaseibacillus paracasei LPH01 against P. acnes, a bacterium that causes acne vulgaris. Fifty-seven Lactobacillus strains were tested for their ability to inhibit P. acnes growth employing the Oxford Cup and double dilution methods. The cell-free supernatant (CFS) of L. paracasei LPH01 demonstrated a strong inhibitory effect, with an inhibition zone diameter of 24...
February 27, 2024: Microbial Pathogenesis
https://read.qxmd.com/read/38417686/enhancement-of-streptomyces-thinghirensis-wae1-for-production-of-bioactive-metabolites-under-different-optimization-strategies
#26
JOURNAL ARTICLE
Mohamed E Osman, Amany A Abo Elnasr, Eslam T Mohamed, Ahmed H I Faraag
Isolation of novel bioactive metabolites from Streptomyces strains is a promising source for drug discovery. However, conventional screening approaches have limitations in identifying new leads due to redundant discoveries. Optimization of culture conditions is important but traditionally optimized one factor at a time, failing to consider interactions. This study addressed these gaps by enhancing metabolite production from Streptomyces thinghirensis WAE1 through statistical optimization. Various chemical and physical factors impacting metabolite production were identified...
February 26, 2024: Microbial Pathogenesis
https://read.qxmd.com/read/38408546/antimicrobial-activity-of-probiotic-bacteria-mediated-cadmium-oxide-nanoparticles-against-fish-pathogens
#27
JOURNAL ARTICLE
Shanmugam Rajeshkumar, Santhoshkumar Jayakodi, M Tharani, Naiyf S Alharbi, Muthu Thiruvengadam
The current research was designed to investigate the antibacterial activity of probiotic bacteria mediated cadmium oxide nanoparticles (CdO NPs) on common fish pathogenic bacteria like Serratia marcescens, Aeromonas hydrophila, Vibrio harveyi, and V. parahaemolyticus. CdO NPs were synthesized using probiotic bacteria as follows: Lactobacillus species with different precursor of cadmium sulfate concentrations (5, 10, and 20 mM). The average crystalline sizes of the CdO NPs were determined based on the XRD patterns using the Debye-Scherrer equation for different precursor concentrations...
February 24, 2024: Microbial Pathogenesis
https://read.qxmd.com/read/38401591/%C3%AE-fur-mutant-as-a-potential-live-attenuated-vaccine-lav-candidate-protects-american-eels-anguilla-rostrata-from-vibrio-harveyi-infection
#28
JOURNAL ARTICLE
Qijuan Wan, Shaowei Zhai, Minxia Chen, Ming Xu, Songlin Guo
The eel farming industry is highly susceptible to Vibriosis. Although various types of vaccines against Vibriosis have been investigated, there is limited research on decreasing the virulence of Vibrions through gene knockout and utilizing it as live attenuated vaccines (LAV). In this study, we aim to develop a LAV candidate against Vibrio harveyi infection in American eels (Anguilla rostrata) using a ferric uptake regulator (fur) gene mutant strain of V. harveyi (Δfur mutant). After the eels were administrated with the Δfur mutant at the dose of 4 × 102  cfu/g body weight, the phagocytic activity of the leucocytes, plasma IgM antibody titers, activity of lysozyme and Superoxide Dismutase (SOD) enzyme, and gene expression levels of 18 immune related proteins were detected to evaluate the protection effect of the LAV...
February 22, 2024: Microbial Pathogenesis
https://read.qxmd.com/read/38395317/evaluation-of-long-term-immune-response-in-cattle-to-botulism-using-a-recombinant-e-coli-bacterin-formulated-with-montanide%C3%A2-isa-50-and-aluminum-hydroxide-adjuvants
#29
JOURNAL ARTICLE
Clovis Moreira, Rafael R Rodrigues, Carlos E P da Cunha, Rafael A Donassolo, Marcos R A Ferreira, Paula F Finger, Hanna G S Oliveira, Karoline P da Cruz, Ângela N Moreira, Felipe M Salvarani, Fabricio R Conceição
Botulism is a severe disease caused by potent botulinum neurotoxins (BoNTs) produced by Clostridium botulinum. This disease is associated with high-lethality outbreaks in cattle, which have been linked to the ingestion of preformed BoNT serotypes C and D, emphasizing the need for effective vaccines. The potency of current commercial toxoids (formaldehyde-inactivated BoNTs) is assured through tests in guinea pigs according to government regulatory guidelines, but their short-term immunity raises concerns. Recombinant vaccines containing the receptor-binding domain have demonstrated potential for eliciting robust protective immunity...
February 21, 2024: Microbial Pathogenesis
https://read.qxmd.com/read/38395316/deletion-of-spea-and-aroc-genes-impacts-the-pathogenicity-of-vibrio-anguillarum-in-spotted-sea-bass
#30
JOURNAL ARTICLE
Haisheng Xie, Yajuan Huang, Shengming Wang, Jingyuan Che, Tuyan Luo, Lekang Li, Baolong Bao
Vibrio anguillarum is one of the major pathogens responsible for bacterial infections in marine environments, causing significant impacts on the aquaculture industry. The misuse of antibiotics leads to bacteria developing multiple drug resistances, which is detrimental to the development of the fisheries industry. In contrast, live attenuated vaccines are gradually gaining acceptance and widespread recognition. In this study, we constructed a double-knockout attenuated strain, V. anguillarum ΔspeA-aroC, to assess its potential for preparing a live attenuated vaccine...
February 21, 2024: Microbial Pathogenesis
https://read.qxmd.com/read/38387848/synthesis-and-characterization-of-marine-seagrass-cymodocea-serrulata-mediated-titanium-dioxide-nanoparticles-for-antibacterial-antibiofilm-and-antioxidant-properties
#31
JOURNAL ARTICLE
Mohankumar Narayanan, Suganthi Srinivasan, Chackaravarthi Gnanasekaran, Govindan Ramachandran, Chenthis Kanisha Chelliah, Rajivgandhi Govindhan, Muthuchamy Maruthupandy, Franck Quero, Wen-Jun Li, Gasim Hayder, Jamal M Khaled, Arulraj Arunachalam, Natesan Manoharan
Cymodocea serrulata mediated titanium dioxide nanoparticles (TiO2 NPs) were successfully synthesized. The XRD pattern and FTIR spectra demonstrated the crystalline structure of TiO2 NPs and the presence of phenols, flavonoids and alkaloids in the extract. Further SEM revealed that TiO2 NPs has uniform structure and spherical in shape with their size ranged from 58 to 117 nm. Antibacterial activity of TiO2 NPs against methicillin-resistant Staphylococcus aureus (MRSA) and Vibrio cholerae (V. cholerae), provided the zone of inhibition of 33...
February 20, 2024: Microbial Pathogenesis
https://read.qxmd.com/read/38387847/comparative-transcriptomics-reveals-common-and-strain-specific-responses-of-human-macrophages-to-infection-with-mycobacterium-tuberculosis-and-mycobacterium-bovis-bcg
#32
JOURNAL ARTICLE
Pei Li, Yang Li, Cun Chuan Wang, Li Gang Xia
Mycobacterium tuberculosis (MTB) and Mycobacterium bovis (M. bovis) are closely related pathogenic mycobacteria known to cause chronic pulmonary infections in both humans and animals. Despite sharing nearly identical genomes and virulence factors, these two bacteria display variations in host tropism, epidemiology, and clinical presentations. M. bovis Bacillus Calmette-Guérin (BCG) is an attenuated strain of M. bovis commonly utilized as a vaccine for tuberculosis (TB). Nevertheless, the molecular underpinnings of these distinctions and the intricacies of host-pathogen interactions remain areas of ongoing research...
February 20, 2024: Microbial Pathogenesis
https://read.qxmd.com/read/38383206/corrigendum-to-kosakonia-cowanii-a-new-bacterial-pathogen-affecting-foxtail-millet-setaria-italica-l-p-beauv-in-china-microb-pathog-181-2023-106201
#33
Yanqing Han, Xi Gao, Guoli Huang, Yindong Chang, Haili Han, Jiao Zhu, Baojun Zhang
No abstract text is available yet for this article.
February 20, 2024: Microbial Pathogenesis
https://read.qxmd.com/read/38382628/combined-analysis-of-the-microbiome-metabolome-and-transcriptome-of-silkie-chickens-in-response-to-avian-pathogenic-e-coli-apec
#34
JOURNAL ARTICLE
Wenqing Li, Wanli Li, Qinghan Zhao, Pinhui Wu, Xinmeng Huang, Wei Jin, Bingxun Wang, Shengli Li, Wei Liu, Guozhi Zhang, Xiangtao Kang
Avian colibacillosis is a bacterial disease caused by avian pathogenic Escherichia coli (APEC) that results in great losses in the poultry industry every year. Individual Silkie chickens of the same breed that are given the same feed in the same feeding conditions have different levels of resistance or susceptibility to APEC. Differences in gut microbes, gut metabolites, and gene expression in the spleen of APEC-resistant and APEC-susceptible chickens were compared, and multiple omics associations were analyzed to explore the mechanism of resistance to APEC in Silkie chickens...
February 19, 2024: Microbial Pathogenesis
https://read.qxmd.com/read/38382627/pre-clinical-safety-and-toxicity-assessment-of-limosilactobacillus-fermentum-ncdc-400-in-murine-model
#35
JOURNAL ARTICLE
Basavaprabhu Haranahalli Nataraj, K Jeevan, Ajay Kumar Dang, Ravinder Nagpal, Syed Azmal Ali, Pradip V Behare
Comprehensive safety assessment of potential probiotic strains is crucial in the selection of risk-free strains for clinical translation. This study aimed to evaluate the biosafety of Limosilactobacillus fermentum NCDC 400, a potential probiotic strain, using oral toxicity tests in a Swiss albino mouse model. Mice were orally gavaged with low (108 CFU/mouse/day) and high (1010 CFU/mouse/day) doses of NCDC 400 for 14 (acute), 28 (subacute), and 90 (subchronic) days to assess behavioral, hematological, biochemical, immunological, and histological effects...
February 19, 2024: Microbial Pathogenesis
https://read.qxmd.com/read/38382626/a-novel-perspective-on-eugenol-as-a-natural-anti-quorum-sensing-molecule-against-serratia-sp
#36
JOURNAL ARTICLE
Fakher Frikha, Marwa Jardak, Sami Aifa, Sami Mnif
Serratia marcescens is commonly noted to be an opportunistic pathogen and is often associated with nosocomial infections. In addition to its high antibiotic resistance, it exhibits a wide range of virulence factors that confer pathogenicity. Targeting quorum sensing (QS) presents a potential therapeutic strategy for treating bacterial infections caused by S. marcescens, as it regulates the expression of various virulence factors. Inhibiting QS can effectively neutralize S. marcescens' bacterial virulence without exerting stress on bacterial growth, facilitating bacterial eradication by the immune system...
February 19, 2024: Microbial Pathogenesis
https://read.qxmd.com/read/38373644/blood-transcriptome-analysis-uncovered-covid-19-myocarditis-crosstalk
#37
JOURNAL ARTICLE
Shuang Liang, Ying-Ying Zheng, Ying Pan
BACKGROUND: The condition of COVID-19-related myocarditis has emerged as a prominent contributor to COVID-19 mortality. As the epidemic persists, its incidence continues to rise. Despite ongoing efforts, the elucidation of COVID-19-related myocarditis underlying molecular mechanisms still requires further investigation. METHODS: Hub genes for COVID-19-related myocarditis were screened by integrating gene expression profile analysis via differential expression in COVID-19 (GSE196822) and myocarditis (GSE148153 and GSE147517)...
February 17, 2024: Microbial Pathogenesis
https://read.qxmd.com/read/38369169/screening-for-immune-biomarkers-associated-with-infection-or-protection-against-ehrlichia-ruminantium-by-rna-sequencing-analysis
#38
JOURNAL ARTICLE
A Pretorius, T Nefefe, N Thema, J Liebenberg, H Steyn, M van Kleef
Heartwater is one of the most economically important tick-borne fatal diseases of livestock. The disease is caused by the bacteria Ehrlichia ruminantium transmitted by Amblyomma ticks. Although there is evidence that interferon-gamma controls E. ruminantium growth and that cellular immune responses are protective, an effective recombinant vaccine for this disease is lacking. Analyses of markers associated with infection as well as protection will lead to a better understanding of the E. ruminantium immune response and corresponding pathways induced in sheep peripheral blood mononuclear cells (PBMC) will assist in development of such a vaccine...
February 16, 2024: Microbial Pathogenesis
https://read.qxmd.com/read/38367848/macrophage-migration-inhibitory-factor-favors-neospora-caninum-infection-in-mice
#39
JOURNAL ARTICLE
Vanessa Resende Souza Silva, Caroline Martins Mota, Lydiane Parreira Maia, Flávia Batista Ferreira, Vanessa Dos Santos Miranda, Neide Maria Silva, Eloísa A Vieira Ferro, José Roberto Mineo, Tiago Wilson Patriarca Mineo
Neospora caninum is a protozoan parasite with worldwide incidence, acting as a major cause of reproductive failures in ruminants and neuromuscular symptoms in dogs. Macrophage Migration Inhibitory Factor (MIF) is produced by several cell types and exhibits a central role in immune responses against intracellular pathogens. The present study aimed to comprehend the role of MIF in the relationship between N. caninum and its host. We used in vivo, in vitro and ex vivo experiments in a model of infection based on genetically deficient mice to analyze the infection kinetics and inflammatory markers...
February 15, 2024: Microbial Pathogenesis
https://read.qxmd.com/read/38364877/evaluation-of-reference-genes-for-gene-expression-analysis-by-real-time-quantitative-pcr-qpcr-in-different-tissues-from-mice-infected-by-ascaris-suum
#40
JOURNAL ARTICLE
Jorge Lucas Nascimento Souza, Camila de Almeida Lopes, Thais Leal-Silva, Flaviane Vieira-Santos, Chiara Cássia Oliveira Amorim, Luiza de Lima Silva Padrão, Ana Rafaela Antunes Porto, Ricardo Toshio Fujiwara, Remo Castro Russo, Lilian Lacerda Bueno
Human ascariasis is the most prevalent helminth infection, affecting 445 million people worldwide. To better understand the impact of the immune system on the pathophysiology of individuals infected with Ascaris suum, mice have been used as experimental models. The RT-qPCR technique is a critical auxiliary tool of investigation used to quantify mRNA levels. However, proper normalization using reference genes is essential to ensure reliable outcomes to avoid analytical errors and false results. Despite the importance of reference genes for experimental A...
February 14, 2024: Microbial Pathogenesis
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