journal
https://read.qxmd.com/read/38483169/diagnostic-accuracy-of-tongue-swab-testing-on-two-automated-tuberculosis-diagnostic-platforms-cepheid-xpert-mtb-rif-ultra-and-molbio-truenat-mtb-ultima
#1
JOURNAL ARTICLE
Rachel C Wood, Angelique K Luabeya, Rane B Dragovich, Alaina M Olson, Katherine A Lochner, Kris M Weigel, Renée Codsi, Humphrey Mulenga, Margaretha de Vos, Mikashmi Kohli, Adam Penn-Nicholson, Mark Hatherill, Gerard A Cangelosi
UNLABELLED: Tongue dorsum swabbing is a potential alternative to sputum collection for tuberculosis (TB) testing. Previous studies showed that Cepheid Xpert MTB/RIF Ultra (Xpert Ultra) can detect Mycobacterium tuberculosis DNA on tongue swabs stored in buffer, with 72% sensitivity and 100% specificity relative to a sputum microbiological reference standard (sputum MRS). The present study evaluated a more convenient sample collection protocol (dry swab storage), combined with streamlined sample processing protocols, for evaluating two commercial TB diagnostic tests: Xpert Ultra and Molbio Truenat MTB Ultima (MTB Ultima)...
March 14, 2024: Journal of Clinical Microbiology
https://read.qxmd.com/read/38477535/evaluation-of-analyte-specific-reagents-for-the-direct-detection-of-pneumocystis-jirovecii
#2
JOURNAL ARTICLE
Samantha R Giffen, Elizabeth Stoeppler, Avian Elliott, Melissa B Miller
UNLABELLED: Pneumocystis jirovecii pneumonia (PJP) is a serious and sometimes fatal infection occurring in immunocompromised individuals. High-risk patients include those with low CD4 counts due to human immunodeficiency virus infection and transplant recipients. The incidence of PJP is increasing, and rapid detection of PJP is needed to effectively target treatment and improve patient outcomes. A common method used is an immunofluorescent assay (IFA), which has limitations, including labor costs, low sensitivity, and requirement for expert interpretation...
March 13, 2024: Journal of Clinical Microbiology
https://read.qxmd.com/read/38470149/clinical-utility-of-multiplex-pcr-in-the-detection-of-pathogens-from-sterile-body-fluids
#3
JOURNAL ARTICLE
Christina R Cox, Kristin N Weghorn, Katie Ruger, Margaret V Powers-Fletcher, Eleanor A Powell, Joel E Mortensen
UNLABELLED: Rapid identification of pathogens in normally sterile body fluid (NSBF) is essential for appropriate patient management, specifically antimicrobial therapy. Limited sensitivity and increased time to detection of traditional culture prompted us to evaluate additional testing to contribute to the diagnosis of infection. The purpose of this study was to evaluate the GenMark Dx ePlex Blood Culture Identification (BCID) Panels on positive body fluids inoculated into blood culture bottles for the detection of microorganisms...
March 12, 2024: Journal of Clinical Microbiology
https://read.qxmd.com/read/38470024/assessment-of-the-performance-of-the-plasma-separation-card-for-hiv-1-viral-load-monitoring-in-south-africa
#4
JOURNAL ARTICLE
Lucy Chimoyi, Lucia Hans, Matthew Oladimeji, Gurpreet Kindra, Karidia Diallo, Kassahun Ayalew, Geoffrey K Setswe, Sergio Carmona
UNLABELLED: Scaling up of newer innovations that address the limitations of the dried blood spot and the logistics of plasma monitoring is needed. We employed a multi-site, cross-sectional assessment of the plasma separation card (PSC) on blood specimens collected from all consenting adults, assenting young and pediatric patients living with HIV from 10 primary healthcare clinics in South Africa. Venous blood for EDTA-plasma samples was collected and analyzed according to the standard of care assay, while collected capillary blood for the PSC samples was analyzed using the Roche COBAS AmpliPrep/Cobas TaqMan (CAP/CTM) HIV-1 Test at the National Reference laboratories...
March 12, 2024: Journal of Clinical Microbiology
https://read.qxmd.com/read/38470023/multicenter-evaluation-of-the-toxoplasma-gondii-real-tm-sacace-kit-performance-for-the-molecular-diagnosis-of-toxoplasmosis
#5
JOURNAL ARTICLE
Juliette Guitard, Marie-Pierre Brenier-Pinchart, Emmanuelle Varlet-Marie, Frédéric Dalle, Celia Rouges, Nicolas Argy, Julie Bonhomme, Agathe Capitaine, Hélène Guégan, Rose-Anne Lavergne, Marie-Laure Dardé, Hervé Pelloux, Florence Robert-Gangneux, Hélène Yera, Yvon Sterkers
The molecular detection of Toxoplasma gondii DNA is a key tool for the diagnosis of disseminated and congenital toxoplasmosis. This multicentric study from the Molecular Biology Pole of the French National Reference Center for toxoplasmosis aimed to evaluate Toxoplasma gondii Real-TM PCR kit (Sacace). The study compared the analytical and clinical performances of this PCR assay with the reference PCRs used in proficient laboratories. PCR efficiencies varied from 90% to 112%; linearity zone extended over four log units (R2 > 0...
March 12, 2024: Journal of Clinical Microbiology
https://read.qxmd.com/read/38470022/the-integration-of-coxiella-burnetii-pcr-testing-in-serum-into-the-diagnostic-algorithm-of-suspected-acute-q-fever-in-an-endemic-setting
#6
JOURNAL ARTICLE
Nesrin Ghanem-Zoubi, Mona Mustafa-Hellou, Maram Zahran, Liat Gazit, Raya Shalaginov, Halima Dabaja-Younis, Moran Szwarcwort
UNLABELLED: Serum polymerase chain reaction (PCR) for the detection of Coxiella burnetii DNA has been suggested for rapid Q fever diagnosis. We evaluated the role of PCR testing in serum in the diagnosis of acute Q fever in an endemic setting. We examined patients suspected of acute Q fever tested for C. burnetii -specific serum real-time PCR in a tertiary hospital between January 2019 toand December 2022. In the first half, PCR orders were consultation-based by infectious diseases specialists, while in the second half, they were guided by serology, positive IgM2, and negative IgG1 and IgG2, indicating early acute infection...
March 12, 2024: Journal of Clinical Microbiology
https://read.qxmd.com/read/38466092/targeted-sequencing-from-cerebrospinal-fluid-for-rapid-identification-of-drug-resistant-tuberculous-meningitis
#7
JOURNAL ARTICLE
Trinh Thi Bich Tram, Le Pham Tien Trieu, Le Thanh Hoang Nhat, Do Dang Anh Thu, Nguyen Le Quang, Nguyen Duc Bang, Tran Thi Hong Chau, Guy E Thwaites, Timothy M Walker, Vu Thi Ngoc Ha, Nguyen Thuy Thuong Thuong
Mortality from tuberculous meningitis (TBM) remains around 30%, with most deaths occurring within 2 months of starting treatment. Mortality from drug-resistant strains is higher still, making early detection of drug resistance (DR) essential. Targeted next-generation sequencing (tNGS) produces high read depths, allowing the detection of DR-associated alleles with low frequencies. We applied Deeplex Myc-TB-a tNGS assay-to cerebrospinal fluid (CSF) samples from 72 adults with microbiologically confirmed TBM and compared its genomic drug susceptibility predictions to a composite reference standard of phenotypic susceptibility testing (pDST) and whole genome sequencing, as well as to clinical outcomes...
March 11, 2024: Journal of Clinical Microbiology
https://read.qxmd.com/read/38457194/cefazolin-as-a-predictor-of-urinary-cephalosporin-activity-in-indicated-enterobacterales
#8
REVIEW
Alexandra L Bryson, Amira Bhalodi, Rachael M Liesman, Amy J Mathers
Traditionally, cephalothin susceptibility results were used to predict the susceptibility of additional cephalosporins; however, in 2013-2014, the Clinical and Laboratory Standards Institute (CLSI) revisited this practice and determined that cefazolin is a more accurate proxy than cephalothin for uncomplicated urinary tract infections (uUTIs). Therefore, a cefazolin surrogacy breakpoint was established to predict the susceptibility of seven oral cephalosporins for Escherichia coli , Klebsiella pneumoniae , and Proteus mirabilis in the context of uUTIs...
March 8, 2024: Journal of Clinical Microbiology
https://read.qxmd.com/read/38456690/prospective-observational-pilot-study-of-the-t2resistance-panel-in-the-t2dx-system-for-detection-of-resistance-genes-in-bacterial-bloodstream-infections
#9
JOURNAL ARTICLE
Thomas J Walsh, Antonella Mencacci, Riccardo Paggi, Evangelia Douka, Charikleia Vrettou, Roger Smith, Oscar Guzman
UNLABELLED: Early initiation of antimicrobial therapy targeting resistant bacterial pathogens causing sepsis and bloodstream infections (BSIs) is critical for a successful outcome. The T2Resistance Panel (T2R) detects the following resistance genes within organisms that commonly cause BSIs directly from patient blood samples: blaKPC , blaCTXM-14/15 , blaNDM /bla/IMP /blaVIM , blaAmpC , blaOXA , vanA , vanB , and mec A/ mec C. We conducted a prospective study in two major medical centers for the detection of circulating resistance genes by T2R in patients with BSIs...
March 8, 2024: Journal of Clinical Microbiology
https://read.qxmd.com/read/38445858/strengthening-bordetella-pertussis-genomic-surveillance-by-direct-sequencing-of-residual-positive-specimens
#10
JOURNAL ARTICLE
Yanhui Peng, Margaret M Williams, Lingzi Xiaoli, Ashley Simon, Heather Fueston, Maria L Tondella, Michael R Weigand
Whole-genome sequencing (WGS) of microbial pathogens recovered from patients with infectious disease facilitates high-resolution strain characterization and molecular epidemiology. However, increasing reliance on culture-independent methods to diagnose infectious diseases has resulted in few isolates available for WGS. Here, we report a novel culture-independent approach to genome characterization of Bordetella pertussis , the causative agent of pertussis and a paradigm for insufficient genomic surveillance due to limited culture of clinical isolates...
March 6, 2024: Journal of Clinical Microbiology
https://read.qxmd.com/read/38441926/closing-the-gap-oxford-nanopore-technologies-r10-sequencing-allows-comparable-results-to-illumina-sequencing-for-snp-based-outbreak-investigation-of-bacterial-pathogens
#11
JOURNAL ARTICLE
Bert Bogaerts, An Van den Bossche, Bavo Verhaegen, Laurence Delbrassinne, Wesley Mattheus, Stéphanie Nouws, Maxime Godfroid, Stefan Hoffman, Nancy H C Roosens, Sigrid C J De Keersmaecker, Kevin Vanneste
Whole-genome sequencing has become the method of choice for bacterial outbreak investigation, with most clinical and public health laboratories currently routinely using short-read Illumina sequencing. Recently, long-read Oxford Nanopore Technologies (ONT) sequencing has gained prominence and may offer advantages over short-read sequencing, particularly with the recent introduction of the R10 chemistry, which promises much lower error rates than the R9 chemistry. However, limited information is available on its performance for bacterial single-nucleotide polymorphism (SNP)-based outbreak investigation...
March 5, 2024: Journal of Clinical Microbiology
https://read.qxmd.com/read/38441056/reply-to-kidd-et-al-inconsistencies-within-the-proposed-framework-for-stabilizing-fungal-nomenclature-risk-further-confusion
#12
JOURNAL ARTICLE
Sybren de Hoog, Thomas J Walsh, Sarah A Ahmed, Ana Alastruey-Izquierdo, Barbara D Alexander, Maiken Cavling Arendrup, Esther Babady, Feng-Yan Bai, Joan-Miquel Balada-Llasat, Andrew Borman, Anuradha Chowdhary, Andrew Clark, Robert C Colgrove, Oliver A Cornely, Tanis C Dingle, Philippe J Dufresne, Jeff Fuller, Jean-Pierre Gangneux, Connie Gibas, Heather Glasgow, Yvonne Graser, Jacques Guillot, Andreas H Groll, Gerhard Haase, Kimberly Hanson, Amanda Harrington, David L Hawksworth, Randall T Hayden, Martin Hoenigl, Vit Hubka, Kristie Johnson, Julianne V Kus, Ruoyu Li, Jacques F Meis, Michaela Lackner, Fanny Lanternier, Sixto M Leal, Francesca Lee, Shawn R Lockhart, Paul Luethy, Isabella Martin, Kyung J Kwon-Chung, Wieland Meyer, M Hong Nguyen, Luis Ostrosky-Zeichner, Elizabeth Palavecino, Preeti Pancholi, Peter G Pappas, Gary W Procop, Scott A Redhead, Daniel D Rhoads, Stefan Riedel, Bryan Stevens, Kaede Ota Sullivan, Paschalis Vergidis, Emmanuel Roilides, Amir Seyedmousavi, Lili Tao, Vania A Vicente, Roxana G Vitale, Qi-Ming Wang, Nancy L Wengenack, Lars Westblade, Nathan Wiederhold, Lewis White, Christina M Wojewoda, Sean X Zhang
No abstract text is available yet for this article.
March 5, 2024: Journal of Clinical Microbiology
https://read.qxmd.com/read/38441055/inconsistencies-within-the-proposed-framework-for-stabilizing-fungal-nomenclature-risk-further-confusion
#13
LETTER
Sarah E Kidd, Ferry Hagen, Catriona L Halliday, Alireza Abdolrasouli, Teun Boekhout, Pedro W Crous, David H Ellis, Juliet Elvy, Graeme N Forrest, Marizeth Groenewald, Rosane C Hahn, Jos Houbraken, Anderson M Rodrigues, James Scott, Tania C Sorrell, Richard C Summerbell, Clement K M Tsui, Andrey Yurkov, Sharon C-A Chen
No abstract text is available yet for this article.
March 5, 2024: Journal of Clinical Microbiology
https://read.qxmd.com/read/38436246/at-home-testing-for-respiratory-viruses-a-minireview-of-the-current-landscape
#14
REVIEW
Laura Smy, Nathan A Ledeboer, Macy G Wood
The landscape of at-home testing using over-the-counter (OTC) tests has been evolving over the last decade. The United States Food and Drug Administration Emergency Use Authorization rule has been in effect since the early 2000s, and it was widely employed during the severe acute respiratory syndrome coronavirus 2 pandemic to authorize antigen and nucleic acid detection tests for use in central laboratories as well as OTC. During the pandemic, the first at-home tests for respiratory viruses became available for consumer use, which opened the door for additional respiratory virus OTC tests...
March 4, 2024: Journal of Clinical Microbiology
https://read.qxmd.com/read/38426762/performance-of-eight-commercial-immunoassays-for-the-detection-of-cytomegalovirus-specific-igm-antibodies-in-pregnancy-no-test-fits-all-needs
#15
JOURNAL ARTICLE
Anja Daiminger, Robert Beck, Simone Exler, Uwe Bartelt, Martin Enders
Detection of cytomegalovirus (CMV)-specific immunoglobulin M (IgM) antibodies as first-line serologic diagnosis plays an important role in identifying CMV primary infection during pregnancy. The performance characteristics of eight commercially available CMV IgM assays were compared. Sensitivity and IgM antibody kinetics were assessed using 100 acute phase and follow-up sera from 39 pregnant women with a well-defined onset of CMV primary infection. Specificity was analyzed using 50 well-characterized serum samples from pregnant women not infected or latently infected with CMV and from patients with other acute infections...
March 1, 2024: Journal of Clinical Microbiology
https://read.qxmd.com/read/38421164/antimicrobial-susceptibilities-of-campylobacter-fetus-report-from-a-reference-laboratory
#16
JOURNAL ARTICLE
Ming May Zhang, Tanner A Melton, Haris Akhtar, Aditya Shah, Audrey N Schuetz, Josh Clement, Ryan W Stevens
UNLABELLED: Campylobacter fetus is known to cause human disease, particularly in elderly and immunocompromised hosts. There are limited published data for antimicrobial susceptibility patterns with this organism, and no interpretive criteria are available. We reviewed antimicrobial susceptibilities of C. fetus isolates tested at a tertiary care center and reference laboratory over an 11-year period. C. fetus isolates from patients treated at Mayo Clinic and those sent as referrals for identification and susceptibility were included...
February 29, 2024: Journal of Clinical Microbiology
https://read.qxmd.com/read/38415638/utilizing-cost-effective-portable-equipment-to-enhance-covid-19-variant-tracking-both-on-site-and-at-a-large-scale
#17
JOURNAL ARTICLE
Sung Yong Park, Gina Faraci, Pamela Ward, Ha Youn Lee
Despite optimistic predictions on the eventual end of COVID-19 (Coronavirus Disease 2019), caution is necessary regarding the emergence of new variants to sustain a positive outlook and effectively address any potential future outbreaks. However, ongoing efforts to track COVID-19 variants are concentrated in developed countries and unique social practices and remote habitats of indigenous peoples present additional challenges. By combining small-sized equipment that is easily accessible and inexpensive, we performed SARS-CoV-2 (Severe Acute Respiratory Syndrome Coronavirus 2) whole genome sequencing and measured the sample-to-answer time and accuracy of this portable variant tracking tool...
February 28, 2024: Journal of Clinical Microbiology
https://read.qxmd.com/read/38415637/rapid-high-throughput-processing-of-tissue-samples-for-microbiological-diagnosis-of-periprosthetic-joint-infections-using-bead-beating-homogenization
#18
JOURNAL ARTICLE
Nathalie Jazmati, Carolin Liebold, Christoph Offerhaus, Annika Volkenand, Stefan Grote, Juliane Pöpsel, Barbara Körber-Irrgang, Thomas Hoppe, Hilmar Wisplinghoff
Enrichment of periprosthetic tissue samples in blood culture bottles (BCBs) for microbiological diagnosis of periprosthetic joint infections (PJI) is more reliable than the use of an enrichment broth. Nevertheless, the extremely time-consuming homogenization of the samples for BCB processing has so far limited its use, especially in high-throughput settings. We aimed to establish a highly scalable homogenization process of tissue samples for long-term incubation in BCBs. A protocol for homogenization of tissue samples using bead beating was established and validated...
February 28, 2024: Journal of Clinical Microbiology
https://read.qxmd.com/read/38407068/a-robust-scalable-and-cost-efficient-approach-to-whole-genome-sequencing-of-rsv-directly-from-clinical-samples
#19
JOURNAL ARTICLE
Sophie Köndgen, Djin-Ye Oh, Andrea Thürmer, Somayyeh Sedaghatjoo, Livia V Patrono, Sébastien Calvignac-Spencer, Barbara Biere, Thorsten Wolff, Ralf Dürrwald, Stephan Fuchs, Janine Reiche
Respiratory syncytial virus (RSV) is a leading cause of acute lower respiratory tract infections causing significant morbidity and mortality among children and the elderly; two RSV vaccines and a monoclonal antibody have recently been approved. Thus, there is an increasing need for a detailed and continuous genomic surveillance of RSV circulating in resource-rich and resource-limited settings worldwide. However, robust, cost-effective methods for whole genome sequencing of RSV from clinical samples that are amenable to high-throughput are still scarce...
February 26, 2024: Journal of Clinical Microbiology
https://read.qxmd.com/read/38393324/this-is-giving-me-a-complex-a-practical-attempt-to-streamline-nontuberculous-mycobacteria-nomenclature-for-clinical-purposes
#20
LETTER
Nancy L Wengenack, Barbara A Brown-Elliott, Nicole M Parrish, Max Salfinger, Christine Y Turenne, Richard J Wallace, Adrian M Zelazny
No abstract text is available yet for this article.
February 23, 2024: Journal of Clinical Microbiology
journal
journal
26018
1
2
Fetch more papers »
Fetching more papers... Fetching...
Remove bar
Read by QxMD icon Read
×

Save your favorite articles in one place with a free QxMD account.

×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"

We want to hear from doctors like you!

Take a second to answer a survey question.