journal
https://read.qxmd.com/read/38014985/the-clinical-epidemiology-management-and-outcomes-of-patients-diagnosed-with-encephalitis-in-north-carolina-2015-2020
#1
JOURNAL ARTICLE
Anuva Fellner, Samuel White, Emmanuel Rockwell, Dana Giandomenico, Monica M Diaz, David J Weber, Melissa B Miller, Ross M Boyce
Despite the relatively high mortality and the difficulty in diagnosis, nearly one-third of patients hospitalized with a documented diagnosis of encephalitis did not undergo a lumbar puncture (LP). When an LP was performed, pathogen-specific testing was greatly underutilized. Infectious etiologies were most common, but over 40% of cases were idiopathic at discharge. These findings suggest that there is a substantial opportunity to improve the quality of care through more accurate and timely diagnosis.
November 28, 2023: Journal of Clinical Microbiology
https://read.qxmd.com/read/38014979/-trichophyton-indotineae-and-other-terbinafine-resistant-dermatophytes-in-north-america
#2
JOURNAL ARTICLE
Shawn R Lockhart, Dallas J Smith, Jeremy A W Gold
Dermatophyte infections (a.k.a. ringworm, tinea) affect an estimated 20%-25% of the world's population. In North America, most dermatophytoses are caused by Trichophyton rubrum or Trichophyton mentagrophytes species complexes. Severe and antifungal-resistant dermatophytoses are a growing global public health problem. A new species of the T. mentagrophytes species complex, Trichophyton indotineae , has recently emerged and is notable for the severe infections it causes, its propensity for antifungal resistance, and its global spread...
November 28, 2023: Journal of Clinical Microbiology
https://read.qxmd.com/read/38014970/universal-pcr-for-bacteria-mycobacteria-and-fungi-a-10-year-retrospective-review-of-clinical-indications-and-patient-outcomes
#3
JOURNAL ARTICLE
Jeffrey Kubiak, Alexandra Morgan, Andrea Kirmaier, Ramy Arnaout, Stefan Riedel
Our work provides a retrospective analysis of universal PCR orders for bacteria, mycobacteria, and fungi across our institution across a 10-year period. We assessed the positivity rates for this diagnostic tool by test type and specimen type and, critically, studied whether and how the results influenced the outcomes from treatment change, to readmission, to death.
November 28, 2023: Journal of Clinical Microbiology
https://read.qxmd.com/read/37991352/evaluation-of-lateral-flow-devices-for-postmortem-rabies-diagnosis-in-animals-in-the-philippines-a-multicenter-study
#4
JOURNAL ARTICLE
Jeffrey L Cruz, Alyssa M Garcia, Nobuo Saito, Maria Glofezita O Lagayan, Rainelda C Dela Peña, Michael S Usana, Steciousneil P Agustin, Judith Z Tattao, Christine V Mamauag, Ofelia P Ducayag, Heather Laxe F Nabus, Princess Diana D L Flores, Ralph Joselle A Fabon, Rogelio P Peñaflor, Dave Christopher G Viñas, Carla A Limsan, Rona P Bernales, Maria Erlinda T Llames, Lerisa E Balopeños, Ramir G Morales, Ana Marie Cristina V Migriño, Orfel June S Calunsag, Josephine J Datoy, Ken Y Palma, Maria Corazon B Sepulveda, Ma Noreen J Eng, Jobienaur S Moscoso, Sheena Mae F Julabar, Jaira D Mauhay, Mark Joseph M Espino, Christine Joyce M Javier, Kazunori Kimitsuki, Akira Nishizono
Expansion of the use of lateral flow devices (LFD) for animal rabies diagnosis can help mitigate the widespread underreporting of rabies. However, this has been hindered by the limited number and small sample size of previous studies. To overcome this limitation, we conducted a multicenter study with a larger sample size to assess the diagnostic accuracy of the ADTEC LFD for postmortem rabies diagnosis in animals. Thirteen governmental animal diagnostic laboratories in the Philippines were involved in this study, and 791 animals suspected of having rabies were tested using both the direct fluorescence antibody test (DFAT) and ADTEC LFD between August 2021 and October 2022...
November 22, 2023: Journal of Clinical Microbiology
https://read.qxmd.com/read/37991351/next-generation-sequencing-for-cytomegalovirus-genotypic-antiviral-resistance-testing
#5
JOURNAL ARTICLE
Heba H Mostafa
Sanger-based sequencing has long served as the gold standard for detecting cytomegalovirus (CMV) resistance mutations. However, next-generation sequencing (NGS) offers a highly multiplexed and sensitive approach. Clinical implementation of NGS-antiviral resistance testing requires thorough validation. In this issue of the Journal of Clinical Microbiology, M. A. Mallory, W. C. Hymas, K. E. Simmon, M. T. Pyne, et al. (J Clin Microbiol 61:e00829-23, 2023, https://doi.org/10.1128/jcm.00829-23) detail a validation of a targeted NGS-based assay for multiple genomic regions that confer resistance to CMV antivirals and share their bioinformatics analysis and reporting pipeline...
November 22, 2023: Journal of Clinical Microbiology
https://read.qxmd.com/read/37982611/real-time-rt-pcr-for-venezuelan-equine-encephalitis-complex-madariaga-and-eastern-equine-encephalitis-viruses-application-in-human-and-mosquito-public-health-surveillance-in-panama
#6
JOURNAL ARTICLE
Jean-Paul Carrera, Dimelza Araúz, Alejandra Rojas, Fátima Cardozo, Victoria Stittleburg, Ingra Morales Claro, Josefrancisco Galue, Carlos Lezcano-Coba, Filipe Romero Rebello Moreira, Luis Felipe -Rivera, Maria Chen-Germán, Brechla Moreno, Zeuz Capitan-Barrios, Sandra López-Vergès, Juan Miguel Pascale, Ester C Sabino, Anayansi Valderrama, Kathryn A Hanley, Christl A Donnelly, Nikos Vasilakis, Nuno R Faria, Jesse J Waggoner
Eastern equine encephalitis virus (EEEV), Madariaga virus (MADV), and Venezuelan equine encephalitis virus complex (VEEV) are New World alphaviruses transmitted by mosquitoes. They cause febrile and sometimes severe neurological diseases in human and equine hosts. Detecting them during the acute phase is hindered by non-specific symptoms and limited diagnostic tools. We designed and clinically assessed real-time reverse transcription polymerase chain reaction assays (rRT-PCRs) for VEEV complex, MADV, and EEEV using whole-genome sequences...
November 20, 2023: Journal of Clinical Microbiology
https://read.qxmd.com/read/37982610/biographical-feature-janet-hindler-mcls-mt-ascp-f-aam
#7
JOURNAL ARTICLE
Romney Humphries
No abstract text is available yet for this article.
November 20, 2023: Journal of Clinical Microbiology
https://read.qxmd.com/read/37971271/antimicrobial-resistance-laboratory-network-s-multisite-evaluation-of-the-thermofisher-sensititre-gn7f-broth-microdilution-panel-for-antimicrobial-susceptibility-testing
#8
JOURNAL ARTICLE
Amelia S Bhatnagar, María-José Machado, Logan Patterson, Karen Anderson, Rebecca L Abelman, Allen Bateman, Angela Biggs, Porscha Bumpus-White, Bradley Craft, Mondraya Howard, Stephen P LaVoie, David Lonsway, Sarah Sabour, Annah Schneider, Paula Snippes-Vagnone, Michael Tran, David Torpey, Ann Valley, Christopher A Elkins, Maria Karlsson, Allison C Brown
In 2017, the Centers for Disease Control and Prevention (CDC) established the Antimicrobial Resistance Laboratory Network to improve domestic detection of multidrug-resistant organisms. CDC and four laboratories evaluated a commercial broth microdilution panel. Antimicrobial susceptibility testing using the Sensititre GN7F (ThermoFisher Scientific, Lenexa, KS) was evaluated by testing 100 CDC and Food and Drug Administration AR Isolate Bank isolates [40 Enterobacterales (ENT), 30 Pseudomonas aeruginosa (PSA), and 30 Acinetobacter baumannii (ACB)]...
November 16, 2023: Journal of Clinical Microbiology
https://read.qxmd.com/read/37966225/multiantigen-print-immunoassay-mapia-for-the-diagnosis-of-neurocysticercosis-a-single-center-diagnostic-optimization-and-accuracy-study-in-lima-peru
#9
JOURNAL ARTICLE
L Toribio, C Guzman, S Noazin, A Zimic-Sheen, M Zimic, I Gonzales, H Saavedra, E J Pretell, J A Bustos, S Handali, H H García
Neurocysticercosis (NCC) is the most common helminthic infection of the human central nervous system. The antibody detection assay of choice is the enzyme-linked immunoelectrotransfer blot assay using lentil-lectin purified parasite antigens (LLGP-EITB, Western blot), an immunoassay with exceptional performance in clinical samples. However, its use is mainly restricted to a few research laboratories because the assay is labor-intensive and requires sophisticated equipment, expertise, and large amounts of parasite material for preparation of reagents...
November 15, 2023: Journal of Clinical Microbiology
https://read.qxmd.com/read/37966224/measuring-beta-lactam-minimum-inhibitory-concentrations-in-staphylococcus-aureus-in-the-clinical-microbiology-laboratory-pinning-the-tail-on-the-donkey
#10
REVIEW
George Sakoulas, Victor Nizet
Significant shortcomings have been identified in standard methods of susceptibility testing in bacteriological media, not only because the media fails to recapitulate the in vivo environment, but susceptibility testing itself fails to capture sub-MIC effects that significantly attenuate bacterial virulence properties. Until susceptibility testing conditions better recapitulate the in vivo environment, attempts to establish the quantitative relevance of beta-lactam MIC using current clinical microbiology standards in Staphylococcus aureus infections will likely prove unsuccessful...
November 15, 2023: Journal of Clinical Microbiology
https://read.qxmd.com/read/37962552/interlaboratory-comparison-of-pseudomonas-aeruginosa-phage-susceptibility-testing
#11
JOURNAL ARTICLE
Krupa Parmar, Lauren Komarow, Damon W Ellison, Andrey A Filippov, Mikeljon P Nikolich, Joseph R Fackler, Martin Lee, Anjna Nair, Priyesh Agrawal, Pranita D Tamma, Maria Souli, Scott R Evans, Kerryl E Greenwood-Quaintance, Scott A Cunningham, Robin Patel
Standardized approaches to phage susceptibility testing (PST) are essential to inform selection of phages for study in patients with bacterial infections. There is no reference standard for assessing bacterial susceptibility to phage. We compared agreement between PST performed at three centers: two centers using a liquid assay standardized between the sites with the third, a plaque assay. Four Pseudomonas aeruginosa phages: PaWRA01ø11 (EPa11), PaWRA01ø39 (EPa39), PaWRA02ø83 (EPa83), PaWRA02ø87 (EPa87), and a cocktail of all four phages were tested against 145 P ...
November 14, 2023: Journal of Clinical Microbiology
https://read.qxmd.com/read/37947408/isothermal-microcalorimetry-improves-accuracy-and-time-to-bacterial-detection-of-periprosthetic-joint-infection-after-total-joint-arthroplasty
#12
JOURNAL ARTICLE
Kyle H Cichos, Randall J Ruark, Elie S Ghanem
Isothermal microcalorimetry (IMC) was evaluated compared to conventional cultures to determine the clinical performance for diagnosing periprosthetic joint infection (PJI) of hip/knee replacements. We prospectively collected three to five deep tissue samples per patient from 152 patients undergoing conversion or revision hip/knee arthroplasty from July 2020 to November 2022. Cultures and IMC for each sample were compared for concordance, median time to detection (TTD), and diagnostic performance based on 2013 Musculoskeletal Infection Society criteria...
November 10, 2023: Journal of Clinical Microbiology
https://read.qxmd.com/read/37933990/development-of-simple-rapid-and-sensitive-methods-for-detection-of-hepatitis-c-virus-rna-from-whole-blood-using-reverse-transcription-loop-mediated-isothermal-amplification
#13
JOURNAL ARTICLE
Matthew D Pauly, Sabrina Weis-Torres, Tonya M Hayden, Lilia M Ganova-Raeva, Saleem Kamili
Hepatitis C virus (HCV) infection is an underdiagnosed global health problem. Diagnosis of current HCV infections typically requires testing for HCV RNA using high-complexity laboratory tests. Methods for the detection of HCV RNA that are simple, inexpensive, rapid, and compatible with use outside of a laboratory setting are very important in order to improve access to hepatitis C diagnostic testing and facilitate accelerated linkage to care. We developed and evaluated three simple workflows for extracting HCV RNA from small volumes of whole blood for use in a sensitive, pan-genotypic RT-LAMP assay...
November 7, 2023: Journal of Clinical Microbiology
https://read.qxmd.com/read/37921475/qiastat-dx-gastrointestinal-panel-and-luminex-xtag-gastrointestinal-pathogen-panel-comparative-evaluation
#14
JOURNAL ARTICLE
Samantha R Giffen, Jacob M Sadler, Melissa B Miller
The diagnosis of acute gastroenteritis is an ongoing clinical challenge in terms of identification of the etiologic agent, time to results, and appropriate treatment. Rapid detection of gastrointestinal pathogens is needed to improve patient care. This study evaluates the performance of the QIAstat-Dx gastrointestinal panel (Q-GP; Investigational Use Only) compared to the Luminex xTAG gastrointestinal pathogen panel (L-GPP; US-IVD). Using 245 stool specimens, we evaluated 10 different targets including rotavirus, norovirus, Salmonella , Shigella , Campylobacter , Giardia , Cryptosporidium , Escherichia coli O157, enterotoxigenic E...
November 3, 2023: Journal of Clinical Microbiology
https://read.qxmd.com/read/37905827/expression-of-concern-for-renesto-et-al-use-of-rpob-gene-analysis-for-detection-and-identification-of-bartonella-species
#15
JOURNAL ARTICLE
(no author information available yet)
No abstract text is available yet for this article.
October 31, 2023: Journal of Clinical Microbiology
https://read.qxmd.com/read/37905814/expression-of-concern-for-yu-et-al-production-of-monoclonal-antibodies-to-tropheryma-whipplei-and-identification-of-recognized-epitopes-by-two-dimensional-electrophoresis-and-mass-spectrometry
#16
JOURNAL ARTICLE
(no author information available yet)
No abstract text is available yet for this article.
October 31, 2023: Journal of Clinical Microbiology
https://read.qxmd.com/read/37905799/expression-of-concern-for-xu-and-raoult-production-of-monoclonal-antibodies-against-rickettsia-massiliae-and-their-use-in-antigenic-and-epidemiological-studies
#17
JOURNAL ARTICLE
(no author information available yet)
No abstract text is available yet for this article.
October 31, 2023: Journal of Clinical Microbiology
https://read.qxmd.com/read/37905795/expression-of-concern-for-fenollar-et-al-bartonella-vinsonii-subsp-arupensis-as-an-agent-of-blood-culture-negative-endocarditis-in-a-human
#18
JOURNAL ARTICLE
(no author information available yet)
No abstract text is available yet for this article.
October 31, 2023: Journal of Clinical Microbiology
https://read.qxmd.com/read/37902331/multi-center-clinical-evaluation-of-the-panther-fusion-sars-cov-2-flu-a-b-rsv-assay-in-nasopharyngeal-swab-specimens-from-symptomatic-individuals
#19
JOURNAL ARTICLE
Anjana Sasidharan, Rangaraj Selvarangan, Kennah Konrad, Matthew L Faron, Salika M Shakir, David Hillyard, Robert K McCall, Ian H McHardy, David C Goldberg, James J Dunn, Alexander L Greninger, Christine Lansang, Rachel Bogh, Carmelle V Remillard
The symptomology is overlapping for respiratory infections due to severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2), influenza A/B viruses, and respiratory syncytial virus (RSV). Accurate detection is essential for proper medical management decisions. This study evaluated the clinical performance of the Panther Fusion SARS-CoV-2/Flu A/B/RSV assay in nasopharyngeal swab (NPS) specimens from individuals of all ages with signs and symptoms of respiratory infection consistent with COVID-19, influenza, or RSV...
October 30, 2023: Journal of Clinical Microbiology
https://read.qxmd.com/read/37902329/-lophomonas-as-a-respiratory-pathogen-jumping-the-gun
#20
REVIEW
Abhishek Mewara, Gillian H Gile, Blaine Mathison, Huan Zhao, Bobbi Pritt, Richard S Bradbury
Human infections with the protozoan Lophomonas have been increasingly reported in the medical literature over the past three decades. Initial reports were based on microscopic identification of the purported pathogen in respiratory specimens. Later, a polymerase chain reaction (PCR) was developed to detect Lophomonas blattarum , following which there has been a significant increase in reports. In this minireview, we thoroughly examine the published reports of Lophomonas infection to evaluate its potential role as a human pathogen...
October 30, 2023: Journal of Clinical Microbiology
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