journal
https://read.qxmd.com/read/36951562/a-brief-update-on-mycobacterial-taxonomy-2020-to-2022
#1
REVIEW
Derek T Armstrong, Emma Eisemann, Nicole Parrish
This brief update provides details regarding two newly described species in the genus Mycobacterium that were identified from humans or associated with human disease and have been validly published for the period January 2020 through October 2022.
March 23, 2023: Journal of Clinical Microbiology
https://read.qxmd.com/read/36946754/potential-of-inaccurate-cefiderocol-susceptibility-results-a-clsi-ast-subcommittee-advisory
#2
LETTER
Patricia J Simner, Elizabeth L Palavecino, Michael J Satlin, Amy J Mathers, Melvin P Weinstein, James S Lewis, Romney Humphries
No abstract text is available yet for this article.
March 22, 2023: Journal of Clinical Microbiology
https://read.qxmd.com/read/36946732/evaluation-of-esat6-cfp10-skin-test-for-mycobacterium-tuberculosis-infection-among-persons-living-with-hiv-in-china
#3
JOURNAL ARTICLE
Peng Lu, Kai Wu, Hongxi Zhou, Haibing Yu, Ju Yuan, Lang Dong, Qiao Liu, Xiaoyan Ding, Wei Lu, Haitao Yang, Limei Zhu, Leonardo Martinez
Recent global guidelines recommend Mycobacterium tuberculosis antigen-based skin tests, such as the ESAT6-CFP10 (EC) skin test, as acceptable alternatives to the tuberculin skin test (TST) and the QuantiFERON-TB Gold In-Tube test (QFT). However, the diagnostic value of these tests among persons living with HIV (PLHIV) is unknown. We aimed to assess the diagnostic accuracy of the EC among a cohort of PLHIV in China. We recruited PLHIV in Jiangsu Province, China, to assess sensitivity and specificity of the EC test...
March 22, 2023: Journal of Clinical Microbiology
https://read.qxmd.com/read/36946723/infection-or-contamination-with-rothia-kocuria-arthrobacter-and-pseudoglutamicibacter-a-retrospective-observational-study-of-non-micrococcus-micrococcaceae-in-the-clinic
#4
JOURNAL ARTICLE
Görel Odeberg, Anna Bläckberg, Torgny Sunnerhagen
Rothia, Kocuria, Arthrobacter, and Pseudoglutamicibacter are bacterial species within the family Micrococcaeae . Knowledge of human infections due to these bacteria is limited. This study aimed to examine features of infections caused by non-Micrococcus Micrococcaeae (NMM). Findings of NMM from blood cultures and other sterile cultures from 2012 to 2021 were identified from the records of the Department of Clinical Microbiology in Region Skåne, Lund, Sweden. Medical records were retrospectively reviewed...
March 22, 2023: Journal of Clinical Microbiology
https://read.qxmd.com/read/36946719/mgit-seq-for-the-identification-of-nontuberculous-mycobacteria-and-drug-resistance-a-prospective-study
#5
JOURNAL ARTICLE
Kiyoharu Fukushima, Yuki Matsumoto, Takanori Matsuki, Haruko Saito, Daisuke Motooka, Sho Komukai, Eriko Fukui, June Yamuchi, Tadayoshi Nitta, Takayuki Niitsu, Yuko Abe, Hiroshi Nabeshima, Yasuharu Nagahama, Takuro Nii, Kazuyuki Tsujino, Keisuke Miki, Seigo Kitada, Atsushi Kumanogoh, Shizuo Akira, Shota Nakamura, Hiroshi Kida
Because nontuberculous mycobacterial pulmonary disease is a considerable health burden, a simple and clinically applicable analytical protocol enabling the identification of subspecies and drug-resistant disease is required to determine the treatment strategy. We aimed to develop a simplified workflow consisting only of direct sequencing of mycobacterial growth indicator tube cultures (MGIT-seq). In total, 138 patients were prospectively enrolled between April 2021 and May 2022, and culture-positive MGIT broths were subjected to sequencing using MinION, a portable next-generation sequencer...
March 22, 2023: Journal of Clinical Microbiology
https://read.qxmd.com/read/36920195/strategies-for-updating-piperacillin-tazobactam-breakpoints
#6
JOURNAL ARTICLE
April M Bobenchik
In this issue of the Journal of Clinical Microbiology , C. Manuel, R. Maynard, A. Abbott, K. Adams, et al. (J Clin Microbiol 61:e01617-22, 2023, https://doi.org/10.1128/JCM.01617-22) describe a multisite study evaluation of piperacillin-tazobactam (TZP) MIC testing on three U.S. Food and Drug Administration (FDA)-cleared antimicrobial susceptibility testing (AST) devices compared to the reference broth microdilution method for organisms belonging to Enterobacterales . Although overall performance of each of the three devices was comparable when applying either FDA or Clinical and Laboratory Standards Institute (CLSI) TZP breakpoints, failure to update to the current CLSI breakpoints may result in falsely categorizing as many as 20% of the TZP-resistant isolates as susceptible...
March 15, 2023: Journal of Clinical Microbiology
https://read.qxmd.com/read/36912659/comparative-evaluation-of-current-biochemical-sequencing-and-proteomic-based-identification-methods-for-the-streptococcus-bovis-group
#7
JOURNAL ARTICLE
Nicole E Putnam, Jung-Ho Youn, Meghan A Wallace, Paul M Luethy, Carey-Ann D Burnham, Susan Butler-Wu, John P Dekker, Anna F Lau
The Streptococcus bovis group (previously group D streptococci) consists of seven distinct species and subspecies. Definitive identification within the group is important, as certain organisms have been associated with gastrointestinal carcinoma, bacteremia, infective endocarditis, meningitis, biliary tract disease, and carcinoma, among others. Definitive identification, however, remains elusive due to limitations and inconsistencies across commonly used identification platforms in the United States. Here, we compared the performance of standard biochemical (Trek Gram-positive identification [GPID] plate, Vitek 2 GPID), sequencing (16S rDNA, sodA ) databases (NCBI, RDP, CDC MicrobeNet), and matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) platforms (Vitek MS, Bruker Biotyper MS) using a set of eight type strains representing all seven strains within the S...
March 13, 2023: Journal of Clinical Microbiology
https://read.qxmd.com/read/36912648/evaluation-of-phenotypic-tests-to-detect-extended-spectrum-%C3%AE-lactamase-esbl-producing-klebsiella-oxytoca-complex-strains
#8
JOURNAL ARTICLE
Edgar I Campos-Madueno, Aline I Moser, Peter M Keller, Vincent Perreten, Laurent Poirel, Patrice Nordmann, Andrea Endimiani
Klebsiella oxytoca complex ( Ko C) species may overproduce their chromosomal class A OXY β-lactamases, conferring reduced susceptibility to piperacillin-tazobactam, expanded-spectrum cephalosporins and aztreonam. Moreover, since clavulanate maintains its ability to inhibit these enzymes, the resulting resistance phenotype may falsely resemble the production of acquired extended-spectrum β-lactamases (ESBLs). In this work, a collection of 44 Ko C strains of human and animal origin was characterized with whole-genome sequencing (WGS) and broth microdilution (BMD) susceptibility testing...
March 13, 2023: Journal of Clinical Microbiology
https://read.qxmd.com/read/36883820/review-of-clinical-and-laboratory-diagnostics-for-coccidioidomycosis
#9
REVIEW
Ian H McHardy, Bridget Barker, George R Thompson
Coccidioidomycosis is a fungal disease associated with soil exposure that frequently goes undiagnosed due at least in part to its nonspecific presentation and the lack of clinical suspicion by health care providers. Currently available diagnostics for coccidioidomycosis offer qualitative results that can suffer from low specificity, while semiquantitative assays are labor-intensive and complex and can require multiple days to complete. Furthermore, significant confusion exists regarding the optimal diagnostic algorithms and appropriate usage of available diagnostic tests...
March 8, 2023: Journal of Clinical Microbiology
https://read.qxmd.com/read/36877080/multicenter-clinical-evaluation-of-etest-eravacycline-for-susceptibility-testing-of-enterobacteriaceae-and-enterococci
#10
JOURNAL ARTICLE
Laurine S Blanchard, Thomas P Armstrong, Michael Kresken, Christopher L Emery, Yun X Ying, Véronique Sauvonnet, Gilles Zambardi
Eravacycline (ERV) (brand name Xerava [Tetraphase]) is a new tetracycline-class antibacterial that has been approved by the U.S. Food and Drug Administration (FDA) and the European Medicines Agency (EMA) for treatment of complicated intra-abdominal infections (cIAIs). ETEST is a gradient diffusion method that represents a simple alternative to the broth microdilution (BMD) method for performing antimicrobial susceptibility testing (AST). A multicenter evaluation of the performance of the new ETEST ERV (bioMérieux) in comparison with BMD was conducted following FDA and International Standards Organization (ISO) recommendations, using FDA- and EUCAST-defined breakpoints...
March 6, 2023: Journal of Clinical Microbiology
https://read.qxmd.com/read/36877025/genome-sequences-from-a-reemergence-of-vibrio-cholerae-in-haiti-2022-reveal-relatedness-to-previously-circulating-strains
#11
LETTER
Cynney Walters, Jessica Chen, Steven Stroika, Lee S Katz, Maryann Turnsek, Valusnor Compère, Monica S Im, Suzanna Gomez, Andre McCullough, Clarissa Landaverde, Jordan Putney, Hayat Caidi, Jason Folster, Heather A Carleton, Jacques Boncy, Christine C Lee
No abstract text is available yet for this article.
March 6, 2023: Journal of Clinical Microbiology
https://read.qxmd.com/read/36877019/prospective-analysis-of-antibody-diagnostic-tests-and-tts1-real-time-pcr-for-diagnosis-of-melioidosis-in-areas-where-it-is-endemic
#12
JOURNAL ARTICLE
Chawitar Noparatvarakorn, Sineenart Sengyee, Atchara Yarasai, Rungnapa Phunpang, Adul Dulsuk, Orawan Ottiwet, Rachan Janon, Chumpol Morakot, Mary N Burtnick, Paul J Brett, T Eoin West, Narisara Chantratita
Melioidosis is a tropical infectious disease caused by Burkholderia pseudomallei. Melioidosis is associated with diverse clinical manifestations and high mortality. Early diagnosis is needed for appropriate treatment, but it takes several days to obtain bacterial culture results. We previously developed a rapid immunochromatography test (ICT) based on hemolysin coregulated protein 1 (Hcp1) and two enzyme-linked immunosorbent assays (ELISAs) based on Hcp1 (Hcp1-ELISA) and O-polysaccharide (OPS-ELISA) for serodiagnosis of melioidosis...
March 6, 2023: Journal of Clinical Microbiology
https://read.qxmd.com/read/36856425/development-and-application-of-real-time-pcr-assay-for-detection-of-salmonella-abortusequi
#13
JOURNAL ARTICLE
Jinhui Wang, Kui Guo, Shuaijie Li, Diqiu Liu, Xiaoyu Chu, Yaoxin Wang, Wei Guo, Cheng Du, Xiaojun Wang, Zhe Hu
Salmonella enterica subsp. enterica serovar Abortusequi is a major pathogen in horse and donkey herds, causing abortion in pregnant equids and resulting in enormous economic losses. A rapid and reliable method is urgently needed to detect S . Abortusequi in herds where the disease is suspected. To achieve this goal, a TaqMan-based real-time PCR assay targeting the gene for the flagellin protein phase 2 antigen FljB was developed. This real-time PCR assay had high specificity, sensitivity, and reproducibility...
March 1, 2023: Journal of Clinical Microbiology
https://read.qxmd.com/read/36853062/evaluation-of-inbios-chagas-detect-fast-a-novel-enzyme-linked-immunosorbent-assay-for-the-detection-of-anti-trypanosoma-cruzi-antibodies
#14
LETTER
Matthew S Moser, Charles J Fleischmann, Emily A Kelly, Rebecca L Townsend, Susan L Stramer, Caryn Bern, Jeffrey D Whitman
No abstract text is available yet for this article.
February 28, 2023: Journal of Clinical Microbiology
https://read.qxmd.com/read/36853034/concordance-of-results-by-three-chagas-disease-antibody-assays-in-u-s-clinical-specimens
#15
LETTER
Matthew S Moser, Charles J Fleischmann, Emily A Kelly, Harry E Prince, Caryn Bern, Jeffrey D Whitman
No abstract text is available yet for this article.
February 28, 2023: Journal of Clinical Microbiology
https://read.qxmd.com/read/36853032/impact-of-rapid-molecular-multiplex-gastrointestinal-pathogen-testing-in-management-of-children-during-a-shigella-outbreak
#16
JOURNAL ARTICLE
N Kanwar, J Jackson, T Bardsley, A Pavia, K M Bourzac, K Holmberg, R Selvarangan
Fecal culture for isolation and identification of Shigella may take days. The BioFire FilmArray Gastrointestinal (GI) panel (bioMérieux, France) is a PCR-based assay that detects enteric pathogens including Shigella /enteroinvasive Escherichia coli (EIEC) in about an hour. The aim of this study was to evaluate the impact of GI panel detection of Shigella in a pediatric emergency department (ED) during an outbreak. Stool samples from children with acute gastroenteritis were tested by the GI panel. Test results were either withheld in preintervention (PRE) or reported to clinicians/families in the postintervention (POST) period...
February 28, 2023: Journal of Clinical Microbiology
https://read.qxmd.com/read/36853028/clinical-evaluation-of-a-new-molecular-test-for-the-detection-of-organisms-causing-vaginitis-and-vaginosis
#17
JOURNAL ARTICLE
Rebecca A Lillis, R Lamar Parker, Ronald Ackerman, Jamie Ackerman, Stephen Young, Alice Weissfeld, Ernest Trevino, Irving Nachamkin, LaShonda Crane, Jacqueline Brown, Christina Huang, Xiaohong Liu, Barbara Van Der Pol
In this prospective, observational, method comparison clinical study, the Xpert Xpress MVP test (MVP) was evaluated using both clinician-collected (CVS) and self-collected vaginal swabs (SVS) collected in a clinical setting. The study was conducted at 12 sites, including point-of-care (POC) settings, from geographically diverse locations in the United States. Participants were biologically female patients ≥ 14 years old with signs and/or symptoms of vaginitis/vaginosis. MVP test results for BV were compared to the BD MAX Vaginal Panel (BDVP)...
February 28, 2023: Journal of Clinical Microbiology
https://read.qxmd.com/read/36853008/performance-of-the-msi-2-database-for-fungal-identification-by-matrix-assisted-laser-desorption-ionization-time-of-flight-mass-spectrometry-from-cleanroom-environments
#18
JOURNAL ARTICLE
Gloria E Trivitt, Anna F Lau
Accurate mold identification by matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) is dependent on robust organism representation in available databases. The Mass Spectrometry Identification (MSI) platform has proven successful for mold identification in clinical and veterinary settings but has yet to be studied with a large set of environmental isolates. Here, we performed a retrospective study using spectra collected by the Bruker MALDI Biotyper (MBT) v4.1 microflex LT instrument to evaluate the MSI-2 database alongside the combined use of the Bruker MBT (including the MBT Filamentous Fungi Library) and the National Institutes of Health (NIH) mold database (MBT/NIH databases)...
February 28, 2023: Journal of Clinical Microbiology
https://read.qxmd.com/read/36852983/performance-of-a-system-for-rapid-phenotypic-antimicrobial-susceptibility-testing-of-gram-negative-bacteria-directly-from-positive-blood-culture-bottles
#19
JOURNAL ARTICLE
J Göransson, M Sundqvist, E Ghaderi, J G Lisby, Y Molin, E Eriksson, S Carlsson, A Cederlöf, L Ellis, J Melin
The rapid administration of optimal antimicrobial treatment is paramount for the treatment of bloodstream infections (BSIs), and rapid antimicrobial susceptibility testing (AST) results are essential. Q-linea has developed the ASTar system, a rapid phenotypic AST device. Here, we report the performance of the ASTar BC G- (Gram-negative) kit when assessed according to the ISO 20776-2:2007 standard for performance evaluation of in vitro diagnostic AST devices. The evaluated ASTar BC G- kit uses a broad panel of 23 antimicrobials for the treatment of BSIs caused by Gram-negative fastidious and nonfastidious bacteria across a range of 6 to 14 2-fold dilutions, including cefoxitin as a screening agent for AmpC-producing Enterobacterales ...
February 28, 2023: Journal of Clinical Microbiology
https://read.qxmd.com/read/36847535/sterility-testing-for-hematopoietic-stem-cells
#20
REVIEW
Tony Cundell, J Wade Atkins, Anna F Lau
Over the last two decades, rapid technological advances have led to the wide adoption of cell and gene therapy products for the treatment of a variety of disease states. In this study, we reviewed the literature between 2003 and 2021 to provide a summary of overarching trends associated with microbial contamination in hematopoietic stem cells (HSCs) derived from peripheral blood, bone marrow, and cord blood. We provide a brief background on the regulatory context for human cells, tissues, and cellular and tissue-based products (HCT/Ps) as regulated by the US Food and Drug Administration (FDA), sterility testing expectations for autologous (Section 361) and allogeneic (Section 351) HSC products, and discuss clinical risks associated with the infusion of a contaminated HSC product...
February 27, 2023: Journal of Clinical Microbiology
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