Distribution of Virulence Genes among Enterococcus species Isolated from Patients of Urinary Tract Infection Attended in a Tertiary Care Hospital of Bangladesh.

Enterococcus species was frequently considered to be commensal organisms but last few decades it has emerged as an important cause of health care associated infections. The presence of virulent genes is one of a key factor for which Enterococcus spp. is gaining attention. In this study, we aim to determine the frequency of virulence genes in uropathogenic Enterococcus species. A total of 46 Enterococcus strains isolated from January 2017 to December 2017. Urine samples were collected from adult clinically suspected urinary tract infected patients from the inpatient and outpatient department of Dhaka Medical College Hospital, Bangladesh irrespective of sex and antibiotic intake. Potential virulence genes such as asa, esp, ace, ebp, cyl, gelE, pilA, pilB, sprE, scm, fms8, ecbA and hyl were detected by PCR using specific primers. Among 46 culture positive Enterococcus, 33(71.74%) were E. faecalis, 11(23.91%) were E. faecium, 2(4.35%) were unidentified. Of the 44 identified Enterococci (33 E. faecalis and 11 E. faecium), 43(97.73%) were positive for pilB, 41(93.18%) for both scm and fms8, 39(88.64%) were positive for ebp, 34(77.27%) for gelE, 32(72.78%) for esp, 31(70.45%) for ecbA, 30(68.18%) for sprE, 28(63.67%) for pilA, 25(56.82%) for ace, 21(47.73%) for cyl, 20(45.45%) for asa and 3(6.82%) for hyl gene. Different virulence factors could be associated with the pathogenicity of E. faecalis and E. faecium and these genes are extensively available among the Enterococcus species.