Evaluation of Matrix-assisted laser desorption/ionization Time-of flight Mass spectrometry (MALDI TOF MS) and VITEK 2 in routine microbial identification.

Anitha Madhavan, Arun Sachu, Nandini Sethuraman, Anil Kumar, Jayalakshmi Vasudevapanicker

Ghana Medical Journal 2021 December

Bacground: Microbial Identification was done by phenotypic methods. VITEK-2 and Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) are now being increasingly used in laboratories.

Objectives: To compare and evaluate the usefulness of MALDI-TOF MS and VITEK-2 in routine microbial identification.

Methods: The performances of MALDI-TOF MS and VITEK 2 were compared for identifying microorganisms.

Results: MALDI- TOF MS and VITEK-2 correctly identified 96 % (96/100) and 97% (97/100) of the isolates upto the genus level.

Conclusion: MALDI TOF MS and VITEK -2 gave comparable identification and error rates. The rapid reduction in turnaround time with MALDI TOF is a significant game-changer in the field of clinical microbiology.

Funding: State Board of Medical Research (SBMR).

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