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Single-color multiplexing by the integration of high-resolution melting pattern recognition with loop-mediated isothermal amplification.
Chemical Communications : Chem Comm 2019 Februrary 9
We develop a single-color multiplexing strategy by the integration of high-resolution melting pattern recognition with loop-mediated isothermal amplification (LAMP). This strategy can identify multiple amplicons with a small DNA melting temperature (Tm) difference (∼0.2 °C) without the involvement of either multicolor labels or parallelized multiplexing, and it can sensitively detect LAMP amplicons with the initial DNA concentrations ranging from 10 to 108 copies.
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